LOCUS       RC234674                6879 bp    DNA     circular  SYN 
DEFINITION  LTF (Myc-DDK tagged) - Homo sapiens lactotransferrin (LTF), transcript variant 2
ACCESSION   NM_001199149
VERSION     NM_001199149.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3036..3127 
                     /label="Myc-DDK"  
     misc_feature    3157..3742 
                     /label="PolyA signal"  
     misc_feature    3214..3233 
                     /label="Restriction Enzyme"  
     misc_feature    4018..4690 
                     /label="ColE1"  
     misc_feature    5120..5914 
                     /label="Kan/Neo"  
     misc_feature    5949..6251 
                     /label="SV40 ori"  
     misc_feature    6337..6814 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3018..3024 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3018 
                     /label="LTF"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAGAAAAGTG CGTGGCCCTC CTGTCAGCTG CATAAAGAGA GACTCCCCCA 
     1081 TCCAGTGTAT CCAGGCCATT GCGGAAAACA GGGCCGATGC TGTGACCCTT GATGGTGGTT 
     1141 TCATATACGA GGCAGGCCTG GCCCCCTACA AACTGCGACC TGTAGCGGCG GAAGTCTACG 
     1201 GGACCGAAAG ACAGCCACGA ACTCACTATT ATGCCGTGGC TGTGGTGAAG AAGGGCGGCA 
     1261 GCTTTCAGCT GAACGAACTG CAAGGTCTGA AGTCCTGCCA CACAGGCCTT CGCAGGACCG 
     1321 CTGGATGGAA TGTCCCTATA GGGACACTTC GTCCATTCTT GAATTGGACG GGTCCACCTG 
     1381 AGCCCATTGA GGCAGCTGTG GCCAGGTTCT TCTCAGCCAG CTGTGTTCCC GGTGCAGATA 
     1441 AAGGACAGTT CCCCAACCTG TGTCGCCTGT GTGCGGGGAC AGGGGAAAAC AAATGTGCCT 
     1501 TCTCCTCCCA GGAACCGTAC TTCAGCTACT CTGGTGCCTT CAAGTGTCTG AGAGACGGGG 
     1561 CTGGAGACGT GGCTTTTATC AGAGAGAGCA CAGTGTTTGA GGACCTGTCA GACGAGGCTG 
     1621 AAAGGGACGA GTATGAGTTA CTCTGCCCAG ACAACACTCG GAAGCCAGTG GACAAGTTCA 
     1681 AAGACTGCCA TCTGGCCCGG GTCCCTTCTC ATGCCGTTGT GGCACGAAGT GTGAATGGCA 
     1741 AGGAGGATGC CATCTGGAAT CTTCTCCGCC AGGCACAGGA AAAGTTTGGA AAGGACAAGT 
     1801 CACCGAAATT CCAGCTCTTT GGCTCCCCTA GTGGGCAGAA AGATCTGCTG TTCAAGGACT 
     1861 CTGCCATTGG GTTTTCGAGG GTGCCCCCGA GGATAGATTC TGGGCTGTAC CTTGGCTCCG 
     1921 GCTACTTCAC TGCCATCCAG AACTTGAGGA AAAGTGAGGA GGAAGTGGCT GCCCGGCGTG 
     1981 CGCGGGTCGT GTGGTGTGCG GTGGGCGAGC AGGAGCTGCG CAAGTGTAAC CAGTGGAGTG 
     2041 GCTTGAGCGA AGGCAGCGTG ACCTGCTCCT CGGCCTCCAC CACAGAGGAC TGCATCGCCC 
     2101 TGGTGCTGAA AGGAGAAGCT GATGCCATGA GTTTGGATGG AGGATATGTG TACACTGCAG 
     2161 GCAAATGTGG TTTGGTGCCT GTCCTGGCAG AGAACTACAA ATCCCAACAA AGCAGTGACC 
     2221 CTGATCCTAA CTGTGTGGAT AGACCTGTGG AAGGATATCT TGCTGTGGCG GTGGTTAGGA 
     2281 GATCAGACAC TAGCCTTACC TGGAACTCTG TGAAAGGCAA GAAGTCCTGC CACACCGCCG 
     2341 TGGACAGGAC TGCAGGCTGG AATATCCCCA TGGGCCTGCT CTTCAACCAG ACGGGCTCCT 
     2401 GCAAATTTGA TGAATATTTC AGTCAAAGCT GTGCCCCTGG GTCTGACCCG AGATCTAATC 
     2461 TCTGTGCTCT GTGTATTGGC GACGAGCAGG GTGAGAATAA GTGCGTGCCC AACAGCAACG 
     2521 AGAGATACTA CGGCTACACT GGGGCTTTCC GGTGCCTGGC TGAGAATGCT GGAGACGTTG 
     2581 CATTTGTGAA AGATGTCACT GTCTTGCAGA ACACTGATGG AAATAACAAT GAGGCATGGG 
     2641 CTAAGGATTT GAAGCTGGCA GACTTTGCGC TGCTGTGCCT CGATGGCAAA CGGAAGCCTG 
     2701 TGACTGAGGC TAGAAGCTGC CATCTTGCCA TGGCCCCGAA TCATGCCGTG GTGTCTCGGA 
     2761 TGGATAAGGT GGAACGCCTG AAACAGGTGT TGCTCCACCA ACAGGCTAAA TTTGGGAGAA 
     2821 ATGGATCTGA CTGCCCGGAC AAGTTTTGCT TATTCCAGTC TGAAACCAAA AACCTTCTGT 
     2881 TCAATGACAA CACTGAGTGT CTGGCCAGAC TCCATGGCAA AACAACATAT GAAAAATATT 
     2941 TGGGACCACA GTATGTCGCA GGCATTACTA ATCTGAAAAA GTGCTCAACC TCCCCCCTCC 
     3001 TGGAAGCCTG TGAATTCCTC AGGAAGACGC GTACGCGGCC GCTCGAGCAG AAACTCATCT 
     3061 CAGAAGAGGA TCTGGCAGCA AATGATATCC TGGATTACAA GGATGACGAC GATAAGGTTT 
     3121 AAACGGCCGG CCGCGGTCAT AGCTGTTTCC TGAACAGATC CCGGGTGGCA TCCCTGTGAC 
     3181 CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA GTTGCCACTC CAGTGCCCAC CAGCCTTGTC 
     3241 CTAATAAAAT TAAGTTGCAT CATTTTGTCT GACTAGGTGT CCTTCTATAA TATTATGGGG 
     3301 TGGAGGGGGG TGGTATGGAG CAAGGGGCAA GTTGGGAAGA CAACCTGTAG GGCCTGCGGG 
     3361 GTCTATTGGG AACCAAGCTG GAGTGCAGTG GCACAATCTT GGCTCACTGC AATCTCCGCC 
     3421 TCCTGGGTTC AAGCGATTCT CCTGCCTCAG CCTCCCGAGT TGTTGGGATT CCAGGCATGC 
     3481 ATGACCAGGC TCAGCTAATT TTTGTTTTTT TGGTAGAGAC GGGGTTTCAC CATATTGGCC 
     3541 AGGCTGGTCT CCAACTCCTA ATCTCAGGTG ATCTACCCAC CTTGGCCTCC CAAATTGCTG 
     3601 GGATTACAGG CGTGAACCAC TGCTCCCTTC CCTGTCCTTC TGATTTTAAA ATAACTATAC 
     3661 CAGCAGGAGG ACGTCCAGAC ACAGCATAGG CTACCTGGCC ATGCCCAACC GGTGGGACAT 
     3721 TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA TGCGTTGGGT CCACTCAGTA GATGCCTGTT 
     3781 GAATTGGGTA CGCGGCCAGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT 
     3841 ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC 
     3901 CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA 
     3961 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA 
     4021 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC 
     4081 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG 
     4141 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC 
     4201 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG 
     4261 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT 
     4321 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT 
     4381 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG 
     4441 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC 
     4501 GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA 
     4561 GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC 
     4621 CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAACC 
     4681 TGAGGCTATG GCAGGGCCTG CCGCCCCGAC GTTGGCTGCG AGCCCTGGGC CTTCACCCGA 
     4741 ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA AACGCGGGCG TATTGGCCCC AATGGGGTCT 
     4801 CGGTGGGGTA TCGACAGAGT GCCAGCCCTG GGACCGAACC CCGCGTTTAT GAACAAACGA 
     4861 CCCAACACCG TGCGTTTTAT TCTGTCTTTT TATTGCCGTC ATAGCGCGGG TTCCTTCCGG 
     4921 TATTGTCTCC TTCCGTGTTT CAGTTAGCCT CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT 
     4981 GAGATCCCCG CGCTGGAGGA TCATCCAGCC GGCGTCCCGG AAAACGATTC CGAAGCCCAA 
     5041 CCTTTCATAG AAGGCGGCGG TGGAATCGAA ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG 
     5101 GTCGGTCATT TCGAACCCCA GAGTCCCGCT CAGAAGAACT CGTCAAGAAG GCGATAGAAG 
     5161 GCGATGCGCT GCGAATCGGG AGCGGCGATA CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT 
     5221 TCGCCGCCAA GCTCTTCAGC AATATCACGG GTAGCCAACG CTATGTCCTG ATAGCGATCC 
     5281 GCCACACCCA GCCGGCCACA GTCGATGAAT CCAGAAAAGC GGCCATTTTC CACCATGATA 
     5341 TTCGGCAAGC AGGCATCGCC ATGGGTCACG ACGAGATCCT CGCCGTCGGG CATGCTCGCC 
     5401 TTGAGCCTGG CGAACAGTTC GGCTGGCGCG AGCCCCTGAT GCTCTTCGTC CAGATCATCC 
     5461 TGATCGACAA GACCGGCTTC CATCCGAGTA CGTGCTCGCT CGATGCGATG TTTCGCTTGG 
     5521 TGGTCGAATG GGCAGGTAGC CGGATCAAGC GTATGCAGCC GCCGCATTGC ATCAGCCATG 
     5581 ATGGATACTT TCTCGGCAGG AGCAAGGTGA GATGACAGGA GATCCTGCCC CGGCACTTCG 
     5641 CCCAATAGCA GCCAGTCCCT TCCCGCTTCA GTGACAACGT CGAGCACAGC TGCGCAAGGA 
     5701 ACGCCCGTCG TGGCCAGCCA CGATAGCCGC GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA 
     5761 CCGGACAGGT CGGTCTTGAC AAAAAGAACC GGGCGCCCCT GCGCTGACAG CCGGAACACG 
     5821 GCGGCATCAG AGCAGCCGAT TGTCTGTTGT GCCCAGTCAT AGCCGAATAG CCTCTCCACC 
     5881 CAAGCGGCCG GAGAACCTGC GTGCAATCCA TCTTGTTCAA TCATGCGAAA CGATCCTCAT 
     5941 CCTGTCTCTT GATCGATCTT TGCAAAAGCC TAGGCCTCCA AAAAAGCCTC CTCACTACTT 
     6001 CTGGAATAGC TCAGAGGCCG AGGCGGCCTC GGCCTCTGCA TAAATAAAAA AAATTAGTCA 
     6061 GCCATGGGGC GGAGAATGGG CGGAACTGGG CGGAGTTAGG GGCGGGATGG GCGGAGTTAG 
     6121 GGGCGGGACT ATGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     6181 GGAGCCTGGG GACTTTCCAC ACCTGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     6241 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCCTAAC TGACACACAT TCCACAGCTG 
     6301 GTTCTTTCCG CCTCAGGACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT 
     6361 TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG 
     6421 CGCACATTTC CCCGAAAAGT GCCACCTGAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG 
     6481 GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT 
     6541 TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT 
     6601 CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT 
     6661 GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG 
     6721 ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC 
     6781 CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA 
     6841 AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTTT
//