LOCUS       RC231464                8097 bp    DNA     circular  SYN 
DEFINITION  TERT (Myc-DDK-tagged)-Human telomerase reverse transcriptase (TERT), transcript variant 2
ACCESSION   NM_001193376
VERSION     NM_001193376.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4252..4343 
                     /label="Myc-DDK"  
     misc_feature    4373..4958 
                     /label="PolyA signal"  
     misc_feature    4430..4449 
                     /label="Restriction Enzyme"  
     misc_feature    5234..5906 
                     /label="ColE1"  
     misc_feature    6336..7130 
                     /label="Kan/Neo"  
     misc_feature    7165..7467 
                     /label="SV40 ori"  
     misc_feature    7553..8030 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4227..4234 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4227 
                     /label="TERT"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCGCGCGCT CCCCGCTGCC GAGCCGTGCG CTCCCTGCTG CGCAGCCACT 
     1081 ACCGCGAGGT GCTGCCGCTG GCCACGTTCG TGCGGCGCCT GGGGCCCCAG GGCTGGCGGC 
     1141 TGGTGCAGCG CGGGGACCCG GCGGCTTTCC GCGCGCTGGT GGCCCAGTGC CTGGTGTGCG 
     1201 TGCCCTGGGA CGCACGGCCG CCCCCCGCCG CCCCCTCCTT CCGCCAGGTG TCCTGCCTGA 
     1261 AGGAGCTGGT GGCCCGAGTG CTGCAGAGGC TGTGCGAGCG CGGCGCGAAG AACGTGCTGG 
     1321 CCTTCGGCTT CGCGCTGCTG GACGGGGCCC GCGGGGGCCC CCCCGAGGCC TTCACCACCA 
     1381 GCGTGCGCAG CTACCTGCCC AACACGGTGA CCGACGCACT GCGGGGGAGC GGGGCGTGGG 
     1441 GGCTGCTGCT GCGCCGCGTG GGCGACGACG TGCTGGTTCA CCTGCTGGCA CGCTGCGCGC 
     1501 TCTTTGTGCT GGTGGCTCCC AGCTGCGCCT ACCAGGTGTG CGGGCCGCCG CTGTACCAGC 
     1561 TCGGCGCTGC CACTCAGGCC CGGCCCCCGC CACACGCTAG TGGACCCCGA AGGCGTCTGG 
     1621 GATGCGAACG GGCCTGGAAC CATAGCGTCA GGGAGGCCGG GGTCCCCCTG GGCCTGCCAG 
     1681 CCCCGGGTGC GAGGAGGCGC GGGGGCAGTG CCAGCCGAAG TCTGCCGTTG CCCAAGAGGC 
     1741 CCAGGCGTGG CGCTGCCCCT GAGCCGGAGC GGACGCCCGT TGGGCAGGGG TCCTGGGCCC 
     1801 ACCCGGGCAG GACGCGTGGA CCGAGTGACC GTGGTTTCTG TGTGGTGTCA CCTGCCAGAC 
     1861 CCGCCGAAGA AGCCACCTCT TTGGAGGGTG CGCTCTCTGG CACGCGCCAC TCCCACCCAT 
     1921 CCGTGGGCCG CCAGCACCAC GCGGGCCCCC CATCCACATC GCGGCCACCA CGTCCCTGGG 
     1981 ACACGCCTTG TCCCCCGGTG TACGCCGAGA CCAAGCACTT CCTCTACTCC TCAGGCGACA 
     2041 AGGAGCAGCT GCGGCCCTCC TTCCTACTCA GCTCTCTGAG GCCCAGCCTG ACTGGCGCTC 
     2101 GGAGGCTCGT GGAGACCATC TTTCTGGGTT CCAGGCCCTG GATGCCAGGG ACTCCCCGCA 
     2161 GGTTGCCCCG CCTGCCCCAG CGCTACTGGC AAATGCGGCC CCTGTTTCTG GAGCTGCTTG 
     2221 GGAACCACGC GCAGTGCCCC TACGGGGTGC TCCTCAAGAC GCACTGCCCG CTGCGAGCTG 
     2281 CGGTCACCCC AGCAGCCGGT GTCTGTGCCC GGGAGAAGCC CCAGGGCTCT GTGGCGGCCC 
     2341 CCGAGGAGGA GGACACAGAC CCCCGTCGCC TGGTGCAGCT GCTCCGCCAG CACAGCAGCC 
     2401 CCTGGCAGGT GTACGGCTTC GTGCGGGCCT GCCTGCGCCG GCTGGTGCCC CCAGGCCTCT 
     2461 GGGGCTCCAG GCACAACGAA CGCCGCTTCC TCAGGAACAC CAAGAAGTTC ATCTCCCTGG 
     2521 GGAAGCATGC CAAGCTCTCG CTGCAGGAGC TGACGTGGAA GATGAGCGTG CGGGACTGCG 
     2581 CTTGGCTGCG CAGGAGCCCA GGGGTTGGCT GTGTTCCGGC CGCAGAGCAC CGTCTGCGTG 
     2641 AGGAGATCCT GGCCAAGTTC CTGCACTGGC TGATGAGTGT GTACGTCGTC GAGCTGCTCA 
     2701 GGTCTTTCTT TTATGTCACG GAGACCACGT TTCAAAAGAA CAGGCTCTTT TTCTACCGGA 
     2761 AGAGTGTCTG GAGCAAGTTG CAAAGCATTG GAATCAGACA GCACTTGAAG AGGGTGCAGC 
     2821 TGCGGGAGCT GTCGGAAGCA GAGGTCAGGC AGCATCGGGA AGCCAGGCCC GCCCTGCTGA 
     2881 CGTCCAGACT CCGCTTCATC CCCAAGCCTG ACGGGCTGCG GCCGATTGTG AACATGGACT 
     2941 ACGTCGTGGG AGCCAGAACG TTCCGCAGAG AAAAGAGGGC CGAGCGTCTC ACCTCGAGGG 
     3001 TGAAGGCACT GTTCAGCGTG CTCAACTACG AGCGGGCGCG GCGCCCCGGC CTCCTGGGCG 
     3061 CCTCTGTGCT GGGCCTGGAC GATATCCACA GGGCCTGGCG CACCTTCGTG CTGCGTGTGC 
     3121 GGGCCCAGGA CCCGCCGCCT GAGCTGTACT TTGTCAAGGT GGATGTGACG GGCGCGTACG 
     3181 ACACCATCCC CCAGGACAGG CTCACGGAGG TCATCGCCAG CATCATCAAA CCCCAGAACA 
     3241 CGTACTGCGT GCGTCGGTAT GCCGTGGTCC AGAAGGCCGC CCATGGGCAC GTCCGCAAGG 
     3301 CCTTCAAGAG CCACGTCTCT ACCTTGACAG ACCTCCAGCC GTACATGCGA CAGTTCGTGG 
     3361 CTCACCTGCA GGAGACCAGC CCGCTGAGGG ATGCCGTCGT CATCGAGCAG AGCTCCTCCC 
     3421 TGAATGAGGC CAGCAGTGGC CTCTTCGACG TCTTCCTACG CTTCATGTGC CACCACGCCG 
     3481 TGCGCATCAG GGGCAAGTCC TACGTCCAGT GCCAGGGGAT CCCGCAGGGC TCCATCCTCT 
     3541 CCACGCTGCT CTGCAGCCTG TGCTACGGCG ACATGGAGAA CAAGCTGTTT GCGGGGATTC 
     3601 GGCGGGACGG GCTGCTCCTG CGTTTGGTGG ATGATTTCTT GTTGGTGACA CCTCACCTCA 
     3661 CCCACGCGAA AACCTTCCTC AGCTATGCCC GGACCTCCAT CAGAGCCAGT CTCACCTTCA 
     3721 ACCGCGGCTT CAAGGCTGGG AGGAACATGC GTCGCAAACT CTTTGGGGTC TTGCGGCTGA 
     3781 AGTGTCACAG CCTGTTTCTG GATTTGCAGG TGAACAGCCT CCAGACGGTG TGCACCAACA 
     3841 TCTACAAGAT CCTCCTGCTG CAGGCGTACA GGTTTCACGC ATGTGTGCTG CAGCTCCCAT 
     3901 TTCATCAGCA AGTTTGGAAG AACCCCACAT TTTTCCTGCG CGTCATCTCT GACACGGCCT 
     3961 CCCTCTGCTA CTCCATCCTG AAAGCCAAGA ACGCAGGGAT GTCGCTGGGG GCCAAGGGCG 
     4021 CCGCCGGCCC TCTGCCCTCC GAGGCCGTGC AGTGGCTGTG CCACCAAGCA TTCCTGCTCA 
     4081 AGCTGACTCG ACACCGTGTC ACCTACGTGC CACTCCTGGG GTCACTCAGG ACAGCCCAGA 
     4141 CGCAGCTGAG TCGGAAGCTC CCGGGGACGA CGCTGACTGC CCTGGAGGCC GCAGCCAACC 
     4201 CGGCACTGCC CTCAGACTTC AAGACCATCC TGGACAGCGG ACCGACGCGT ACGCGGCCGC 
     4261 TCGAGCAGAA ACTCATCTCA GAAGAGGATC TGGCAGCAAA TGATATCCTG GATTACAAGG 
     4321 ATGACGACGA TAAGGTTTAA ACGGCCGGCC GCGGTCATAG CTGTTTCCTG AACAGATCCC 
     4381 GGGTGGCATC CCTGTGACCC CTCCCCAGTG CCTCTCCTGG CCCTGGAAGT TGCCACTCCA 
     4441 GTGCCCACCA GCCTTGTCCT AATAAAATTA AGTTGCATCA TTTTGTCTGA CTAGGTGTCC 
     4501 TTCTATAATA TTATGGGGTG GAGGGGGGTG GTATGGAGCA AGGGGCAAGT TGGGAAGACA 
     4561 ACCTGTAGGG CCTGCGGGGT CTATTGGGAA CCAAGCTGGA GTGCAGTGGC ACAATCTTGG 
     4621 CTCACTGCAA TCTCCGCCTC CTGGGTTCAA GCGATTCTCC TGCCTCAGCC TCCCGAGTTG 
     4681 TTGGGATTCC AGGCATGCAT GACCAGGCTC AGCTAATTTT TGTTTTTTTG GTAGAGACGG 
     4741 GGTTTCACCA TATTGGCCAG GCTGGTCTCC AACTCCTAAT CTCAGGTGAT CTACCCACCT 
     4801 TGGCCTCCCA AATTGCTGGG ATTACAGGCG TGAACCACTG CTCCCTTCCC TGTCCTTCTG 
     4861 ATTTTAAAAT AACTATACCA GCAGGAGGAC GTCCAGACAC AGCATAGGCT ACCTGGCCAT 
     4921 GCCCAACCGG TGGGACATTT GAGTTGCTTG CTTGGCACTG TCCTCTCATG CGTTGGGTCC 
     4981 ACTCAGTAGA TGCCTGTTGA ATTGGGTACG CGGCCAGCGG CGAGCGGTAT CAGCTCACTC 
     5041 AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC 
     5101 AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG 
     5161 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC 
     5221 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT 
     5281 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT 
     5341 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG 
     5401 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT 
     5461 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT 
     5521 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG 
     5581 CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA 
     5641 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT 
     5701 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC 
     5761 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT 
     5821 ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA 
     5881 AAGTATATAT GAGTAACCTG AGGCTATGGC AGGGCCTGCC GCCCCGACGT TGGCTGCGAG 
     5941 CCCTGGGCCT TCACCCGAAC TTGGGGGGTG GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA 
     6001 TTGGCCCCAA TGGGGTCTCG GTGGGGTATC GACAGAGTGC CAGCCCTGGG ACCGAACCCC 
     6061 GCGTTTATGA ACAAACGACC CAACACCGTG CGTTTTATTC TGTCTTTTTA TTGCCGTCAT 
     6121 AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG 
     6181 GGCGAAGAAC TCCAGCATGA GATCCCCGCG CTGGAGGATC ATCCAGCCGG CGTCCCGGAA 
     6241 AACGATTCCG AAGCCCAACC TTTCATAGAA GGCGGCGGTG GAATCGAAAT CTCGTGATGG 
     6301 CAGGTTGGGC GTCGCTTGGT CGGTCATTTC GAACCCCAGA GTCCCGCTCA GAAGAACTCG 
     6361 TCAAGAAGGC GATAGAAGGC GATGCGCTGC GAATCGGGAG CGGCGATACC GTAAAGCACG 
     6421 AGGAAGCGGT CAGCCCATTC GCCGCCAAGC TCTTCAGCAA TATCACGGGT AGCCAACGCT 
     6481 ATGTCCTGAT AGCGATCCGC CACACCCAGC CGGCCACAGT CGATGAATCC AGAAAAGCGG 
     6541 CCATTTTCCA CCATGATATT CGGCAAGCAG GCATCGCCAT GGGTCACGAC GAGATCCTCG 
     6601 CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG AACAGTTCGG CTGGCGCGAG CCCCTGATGC 
     6661 TCTTCGTCCA GATCATCCTG ATCGACAAGA CCGGCTTCCA TCCGAGTACG TGCTCGCTCG 
     6721 ATGCGATGTT TCGCTTGGTG GTCGAATGGG CAGGTAGCCG GATCAAGCGT ATGCAGCCGC 
     6781 CGCATTGCAT CAGCCATGAT GGATACTTTC TCGGCAGGAG CAAGGTGAGA TGACAGGAGA 
     6841 TCCTGCCCCG GCACTTCGCC CAATAGCAGC CAGTCCCTTC CCGCTTCAGT GACAACGTCG 
     6901 AGCACAGCTG CGCAAGGAAC GCCCGTCGTG GCCAGCCACG ATAGCCGCGC TGCCTCGTCT 
     6961 TGCAGTTCAT TCAGGGCACC GGACAGGTCG GTCTTGACAA AAAGAACCGG GCGCCCCTGC 
     7021 GCTGACAGCC GGAACACGGC GGCATCAGAG CAGCCGATTG TCTGTTGTGC CCAGTCATAG 
     7081 CCGAATAGCC TCTCCACCCA AGCGGCCGGA GAACCTGCGT GCAATCCATC TTGTTCAATC 
     7141 ATGCGAAACG ATCCTCATCC TGTCTCTTGA TCGATCTTTG CAAAAGCCTA GGCCTCCAAA 
     7201 AAAGCCTCCT CACTACTTCT GGAATAGCTC AGAGGCCGAG GCGGCCTCGG CCTCTGCATA 
     7261 AATAAAAAAA ATTAGTCAGC CATGGGGCGG AGAATGGGCG GAACTGGGCG GAGTTAGGGG 
     7321 CGGGATGGGC GGAGTTAGGG GCGGGACTAT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     7381 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CTGGTTGCTG ACTAATTGAG 
     7441 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCCTAACTG 
     7501 ACACACATTC CACAGCTGGT TCTTTCCGCC TCAGGACTCT TCCTTTTTCA ATATTATTGA 
     7561 AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT 
     7621 AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGC GCCCTGTAGC 
     7681 GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC 
     7741 GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT 
     7801 CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC 
     7861 CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG 
     7921 ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA 
     7981 ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG 
     8041 ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTT
//