LOCUS       RC231422                7368 bp    DNA     circular  SYN 
DEFINITION  PIWIL1 (Myc-DDK-tagged)-Human piwi-like 1 (Drosophila) (PIWIL1), transcript variant 2
ACCESSION   NM_001190971
VERSION     NM_001190971.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3525..3616 
                     /label="Myc-DDK"  
     misc_feature    3646..4231 
                     /label="PolyA signal"  
     misc_feature    3703..3722 
                     /label="Restriction Enzyme"  
     misc_feature    4507..5179 
                     /label="ColE1"  
     misc_feature    5609..6403 
                     /label="Kan/Neo"  
     misc_feature    6438..6740 
                     /label="SV40 ori"  
     misc_feature    6826..7303 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3507..3513 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3507 
                     /label="PIWIL1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GACTGGGAGA GCCCGAGCCA GAGCCAGAGG AAGGGCCCGC GGTCAGGAGA 
     1081 CAGCGCAGCT GGTGGGCTCC ACTGCCAGTC AGCAACCTGG TTATATTCAG CCTAGGCCTC 
     1141 AGCCGCCACC AGCAGAGGGG GAATTATTTG GCCGTGGACG GCAGAGAGGA ACAGCAGGAG 
     1201 GAACAGCCAA GTCACAAGGA CTCCAGATAT CTGCTGGATT TCAGGAGTTA TCGTTAGCAG 
     1261 AGAGAGGAGG TCGTCGTAGA GATTTTCATG ATCTTGGTGT GAATACAAGG CAGAACCTAG 
     1321 ACCATGTTAA AGAATCAAAA ACAGGTTCTT CAGGCATTAT AGTAAGGTTA AGCACTAACC 
     1381 ATTTCCGGCT GACATCCCGT CCCCAGTGGG CCTTATATCA GTATCACATT GACTATAACC 
     1441 CACTGATGGA AGCCAGAAGA CTCCGTTCAG CTCTTCTTTT TCAACACGAA GATCTAATTG 
     1501 GAAAGTGTCA TGCTTTTGAT GGAACGATAT TATTTTTACC TAAAAGACTA CAGCAAAAGG 
     1561 TTACTGAAGT TTTTAGTAAG ACCCGGAATG GAGAGGATGT GAGGATAACG ATCACTTTAA 
     1621 CAAATGAACT TCCACCTACA TCACCAACTT GTTTGCAGTT CTATAATATT ATTTTCAGGA 
     1681 GGCTTTTGAA AATCATGAAT TTGCAACAAA TTGGACGAAA TTATTATAAC CCAAATGACC 
     1741 CAATTGATAT TCCAAGTCAC AGGTTGGTGA TTTGGCCTGG CTTCACTACT TCCATCCTTC 
     1801 AGTATGAAAA CAGCATCATG CTCTGCACTG ACGTTAGCCA TAAAGTCCTT CGAAGTGAGA 
     1861 CTGTTTTGGA TTTCATGTTC AACTTTTATC ATCAGACAGA AGAACATAAA TTTCAAGAAC 
     1921 AAGTTTCCAA AGAACTAATA GGTTTAGTTG TTCTTACCAA GTATAACAAT AAGACATACA 
     1981 GAGTGGATGA TATTGACTGG GACCAGAATC CCAAGAGCAC CTTTAAGAAA GCCGACGGCT 
     2041 CTGAAGTCAG CTTCTTAGAA TACTACAGGA AGCAATACAA CCAAGAGATC ACCGACTTGA 
     2101 AGCAGCCTGT CTTGGTCAGC CAGCCCAAGA GAAGGCGGGG CCCTGGGGGG ACACTGCCAG 
     2161 GGCCTGCCAT GCTCATTCCT GAGCTCTGCT ATCTTACAGG TCTAACTGAT AAAATGCGTA 
     2221 ATGATTTTAA CGTGATGAAA GACTTAGCCG TTCATACAAG ACTAACTCCA GAGCAAAGGC 
     2281 AGCGTGAAGT GGGACGACTC ATTGATTACA TTCATAAAAA CGATAATGTT CAAAGGGAGC 
     2341 TTCGAGACTG GGGTTTGAGC TTTGATTCCA ACTTACTGTC CTTCTCAGGA AGAATTTTGC 
     2401 AAACAGAAAA GATTCACCAA GGTGGAAAAA CATTTGATTA CAATCCACAA TTTGCAGATT 
     2461 GGTCCAAAGA AACAAGAGGT GCACCATTAA TTAGTGTTAA GCCACTAGAT AACTGGCTGT 
     2521 TGATCTATAC GCGAAGAAAT TATGAAGCAG CCAATTCATT GATACAAAAT CTATTTAAAG 
     2581 TTACACCAGC CATGGGCATG CAAATGAGAA AAGCAATAAT GATTGAAGTG GATGACAGAA 
     2641 CTGAAGCCTA CTTAAGAGTC TTACAGCAAA AGGTCACAGC AGACACCCAG ATAGTTGTCT 
     2701 GTCTGTTGTC AAGTAATCGG AAGGACAAAT ACGATGCTAT TAAAAAATAC CTGTGTACAG 
     2761 ATTGCCCTAC CCCAAGTCAG TGTGTGGTGG CCCGAACCTT AGGCAAACAG CAAACTGTCA 
     2821 TGGCCATTGC TACAAAGATT GCCCTACAGA TGAACTGCAA GATGGGAGGA GAGCTCTGGA 
     2881 GGGTGGACAT CCCCCTGAAG CTCGTGATGA TCGTTGGCAT CGATTGTTAC CATGACATGA 
     2941 CAGCTGGGCG GAGGTCAATC GCAGGATTTG TTGCCAGCAT CAATGAAGGG ATGACCCGCT 
     3001 GGTTCTCACG CTGCATATTT CAGGATAGAG GACAGGAGCT GGTAGATGGG CTCAAAGTCT 
     3061 GCCTGCAAGC GGCTCTGAGG GCTTGGAATA GCTGCAATGA GTACATGCCC AGCCGGATCA 
     3121 TCGTGTACCG CGATGGCGTA GGAGACGGCC AGCTGAAAAC ACTGGTGAAC TACGAAGTGC 
     3181 CACAGTTTTT GGATTGTCTA AAATCCATTG GTAGAGGTTA CAACCCTAGA CTAACGGTAA 
     3241 TTGTGGTGAA GAAAAGAGTG AACACCAGAT TTTTTGCTCA GTCTGGAGGA AGACTTCAGA 
     3301 ATCCACTTCC TGGAACAGTT ATTGATGTAG AGGTTACCAG ACCAGAATGG TATGACTTTT 
     3361 TTATCGTGAG CCAGGCTGTG AGAAGTGGTA GTGTTTCTCC CACACATTAC AATGTCATCT 
     3421 ATGACAACAG CGGCCTGAAG CCAGACCACA TACAGCGCTT GACCTACAAG CTGTGCCACA 
     3481 TCTATTACAA CTGGCCAGTA AGTGCTTCTA CTTGTACGCG TACGCGGCCG CTCGAGCAGA 
     3541 AACTCATCTC AGAAGAGGAT CTGGCAGCAA ATGATATCCT GGATTACAAG GATGACGACG 
     3601 ATAAGGTTTA AACGGCCGGC CGCGGTCATA GCTGTTTCCT GAACAGATCC CGGGTGGCAT 
     3661 CCCTGTGACC CCTCCCCAGT GCCTCTCCTG GCCCTGGAAG TTGCCACTCC AGTGCCCACC 
     3721 AGCCTTGTCC TAATAAAATT AAGTTGCATC ATTTTGTCTG ACTAGGTGTC CTTCTATAAT 
     3781 ATTATGGGGT GGAGGGGGGT GGTATGGAGC AAGGGGCAAG TTGGGAAGAC AACCTGTAGG 
     3841 GCCTGCGGGG TCTATTGGGA ACCAAGCTGG AGTGCAGTGG CACAATCTTG GCTCACTGCA 
     3901 ATCTCCGCCT CCTGGGTTCA AGCGATTCTC CTGCCTCAGC CTCCCGAGTT GTTGGGATTC 
     3961 CAGGCATGCA TGACCAGGCT CAGCTAATTT TTGTTTTTTT GGTAGAGACG GGGTTTCACC 
     4021 ATATTGGCCA GGCTGGTCTC CAACTCCTAA TCTCAGGTGA TCTACCCACC TTGGCCTCCC 
     4081 AAATTGCTGG GATTACAGGC GTGAACCACT GCTCCCTTCC CTGTCCTTCT GATTTTAAAA 
     4141 TAACTATACC AGCAGGAGGA CGTCCAGACA CAGCATAGGC TACCTGGCCA TGCCCAACCG 
     4201 GTGGGACATT TGAGTTGCTT GCTTGGCACT GTCCTCTCAT GCGTTGGGTC CACTCAGTAG 
     4261 ATGCCTGTTG AATTGGGTAC GCGGCCAGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT 
     4321 AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA 
     4381 GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC 
     4441 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT 
     4501 ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT 
     4561 GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG 
     4621 CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA 
     4681 CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA 
     4741 CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC 
     4801 GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG 
     4861 AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG 
     4921 TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA 
     4981 GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC 
     5041 TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG 
     5101 GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA 
     5161 TGAGTAACCT GAGGCTATGG CAGGGCCTGC CGCCCCGACG TTGGCTGCGA GCCCTGGGCC 
     5221 TTCACCCGAA CTTGGGGGGT GGGGTGGGGA AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA 
     5281 ATGGGGTCTC GGTGGGGTAT CGACAGAGTG CCAGCCCTGG GACCGAACCC CGCGTTTATG 
     5341 AACAAACGAC CCAACACCGT GCGTTTTATT CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT 
     5401 TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA 
     5461 CTCCAGCATG AGATCCCCGC GCTGGAGGAT CATCCAGCCG GCGTCCCGGA AAACGATTCC 
     5521 GAAGCCCAAC CTTTCATAGA AGGCGGCGGT GGAATCGAAA TCTCGTGATG GCAGGTTGGG 
     5581 CGTCGCTTGG TCGGTCATTT CGAACCCCAG AGTCCCGCTC AGAAGAACTC GTCAAGAAGG 
     5641 CGATAGAAGG CGATGCGCTG CGAATCGGGA GCGGCGATAC CGTAAAGCAC GAGGAAGCGG 
     5701 TCAGCCCATT CGCCGCCAAG CTCTTCAGCA ATATCACGGG TAGCCAACGC TATGTCCTGA 
     5761 TAGCGATCCG CCACACCCAG CCGGCCACAG TCGATGAATC CAGAAAAGCG GCCATTTTCC 
     5821 ACCATGATAT TCGGCAAGCA GGCATCGCCA TGGGTCACGA CGAGATCCTC GCCGTCGGGC 
     5881 ATGCTCGCCT TGAGCCTGGC GAACAGTTCG GCTGGCGCGA GCCCCTGATG CTCTTCGTCC 
     5941 AGATCATCCT GATCGACAAG ACCGGCTTCC ATCCGAGTAC GTGCTCGCTC GATGCGATGT 
     6001 TTCGCTTGGT GGTCGAATGG GCAGGTAGCC GGATCAAGCG TATGCAGCCG CCGCATTGCA 
     6061 TCAGCCATGA TGGATACTTT CTCGGCAGGA GCAAGGTGAG ATGACAGGAG ATCCTGCCCC 
     6121 GGCACTTCGC CCAATAGCAG CCAGTCCCTT CCCGCTTCAG TGACAACGTC GAGCACAGCT 
     6181 GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC GATAGCCGCG CTGCCTCGTC TTGCAGTTCA 
     6241 TTCAGGGCAC CGGACAGGTC GGTCTTGACA AAAAGAACCG GGCGCCCCTG CGCTGACAGC 
     6301 CGGAACACGG CGGCATCAGA GCAGCCGATT GTCTGTTGTG CCCAGTCATA GCCGAATAGC 
     6361 CTCTCCACCC AAGCGGCCGG AGAACCTGCG TGCAATCCAT CTTGTTCAAT CATGCGAAAC 
     6421 GATCCTCATC CTGTCTCTTG ATCGATCTTT GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC 
     6481 TCACTACTTC TGGAATAGCT CAGAGGCCGA GGCGGCCTCG GCCTCTGCAT AAATAAAAAA 
     6541 AATTAGTCAG CCATGGGGCG GAGAATGGGC GGAACTGGGC GGAGTTAGGG GCGGGATGGG 
     6601 CGGAGTTAGG GGCGGGACTA TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     6661 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCTGGTTGCT GACTAATTGA GATGCATGCT 
     6721 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCCTAACT GACACACATT 
     6781 CCACAGCTGG TTCTTTCCGC CTCAGGACTC TTCCTTTTTC AATATTATTG AAGCATTTAT 
     6841 CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA 
     6901 GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG CGCCCTGTAG CGGCGCATTA 
     6961 AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG 
     7021 CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA 
     7081 GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC 
     7141 AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT 
     7201 CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA 
     7261 ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC 
     7321 TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTT
//