LOCUS       RC230893                8421 bp    DNA     circular  SYN 
DEFINITION  NRCAM (Myc-DDK-tagged)-Human neuronal cell adhesion molecule (NRCAM), transcript variant 6
ACCESSION   NM_001193584
VERSION     NM_001193584.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4578..4669 
                     /label="Myc-DDK"  
     misc_feature    4699..5284 
                     /label="PolyA signal"  
     misc_feature    4756..4775 
                     /label="Restriction Enzyme"  
     misc_feature    5560..6232 
                     /label="ColE1"  
     misc_feature    6662..7456 
                     /label="Kan/Neo"  
     misc_feature    7491..7793 
                     /label="SV40 ori"  
     misc_feature    7879..8356 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4560..4566 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4560 
                     /label="NRCAM"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCAGCTTAAA ATAATGCCGA AAAAGAAGCG CTTATCTGCG GGCAGAGTGC 
     1081 CCCTGATTCT CTTCCTGTGC CAGATGATTA GTGCACTGGA AGTACCTCTT GATCCAAAAC 
     1141 TTCTTGAAGA CTTGGTACAG CCTCCAACCA TCACCCAACA GTCTCCAAAA GATTACATTA 
     1201 TTGACCCTCG GGAGAATATT GTAATCCAGT GTGAAGCCAA AGGGAAACCG CCCCCAAGCT 
     1261 TTTCCTGGAC CCGTAATGGG ACTCATTTTG ACATCGATAA AGACCCTCTG GTCACCATGA 
     1321 AGCCTGGCAC AGGAACGCTC ATAATTAACA TCATGAGCGA AGGGAAAGCT GAGACCTATG 
     1381 AAGGAGTCTA TCAGTGTACA GCAAGGAACG AACGCGGAGC TGCAGTTTCT AATAACATTG 
     1441 TTGTCCGCCC ATCCAGATCA CCATTGTGGA CCAAAGAAAA ACTTGAACCA ATCACACTTC 
     1501 AAAGTGGTCA GTCTTTAGTA CTTCCCTGCA GACCCCCAAT TGGATTACCA CCACCTATAA 
     1561 TATTTTGGAT GGATAATTCC TTTCAAAGAC TTCCACAAAG TGAGAGAGTT TCTCAAGGTT 
     1621 TGAATGGGGA CCTTTATTTT TCCAATGTCC TCCCAGAGGA CACCCGCGAA GACTATATCT 
     1681 GTTATGCTAG ATTTAATCAT ACTCAAACCA TACAGCAGAA GCAACCTATT TCTGTGAAGG 
     1741 TGATTTCAGC TAAATCAAGT AGAGAGAGGC CACCAACATT TTTAACTCCA GAAGGCAATG 
     1801 CAAGTAACAA AGAGGAATTA AGAGGAAATG TGCTTTCACT GGAGTGCATT GCAGAAGGAC 
     1861 TGCCTACCCC AATTATTTAC TGGGCAAAGG AAGATGGAAT GCTACCCAAA AACAGGACAG 
     1921 TTTATAAGAA CTTTGAGAAA ACCTTGCAGA TCATTCATGT TTCAGAAGCA GACTCTGGAA 
     1981 ATTACCAATG TATAGCAAAA AATGCATTAG GAGCCATCCA CCATACCATT TCTGTTAGAG 
     2041 TTAAAGCGGC TCCATACTGG ATCACAGCCC CTCAAAATCT TGTGCTGTCC CCAGGAGAGG 
     2101 ATGGGACCTT GATCTGCAGA GCTAATGGCA ACCCCAAACC CAGAATTAGC TGGTTAACAA 
     2161 ATGGAGTCCC AATAGAAATT GCCCCTGATG ACCCCAGCAG AAAAATAGAT GGCGATACCA 
     2221 TTATTTTTTC AAATGTTCAA GAAAGATCAA GTGCAGTATA TCAGTGCAAT GCCTCTAATG 
     2281 AATATGGATA TTTACTGGCA AACGCATTTG TAAATGTGCT GGCTGAGCCA CCACGAATCC 
     2341 TCACACCTGC AAACACACTC TACCAGGTCA TTGCAAACAG GCCTGCTTTA CTAGACTGTG 
     2401 CCTTCTTTGG GTCTCCTCTC CCAACCATCG AGTGGTTTAA AGGAGCTAAA GGAAGTGCTC 
     2461 TTCATGAAGA TATTTATGTT TTACATGAAA ATGGAACTTT GGAAATTCCT GTGGCCCAAA 
     2521 AGGACAGTAC AGGAACTTAT ACGTGTGTTG CAAGGAATAA ATTAGGGATG GCAAAGAATG 
     2581 AAGTTCACTT AGAAATCAAA GATGCTACAT GGATCGTTAA ACAGCCCGAA TATGCAGTTG 
     2641 TGCAAAGAGG GAGCATGGTG TCCTTTGAAT GCAAAGTGAA ACATGATCAC ACCTTATCCC 
     2701 TCACTGTCCT GTGGCTGAAG GACAACAGGG AACTGCCCAG TGATGAAAGG TTCACTGTTG 
     2761 ACAAGGATCA TCTAGTGGTA GCTGATGTCA GTGACGATGA CAGCGGGACC TACACGTGTG 
     2821 TGGCCAACAC CACTCTGGAC AGCGTCTCCG CCAGCGCTGT GCTTAGCGTT GTTGCTCCTA 
     2881 CTCCAACTCC AGCTCCCGTT TACGATGTCC CAAATCCTCC CTTTGACTTA GAACTGACAG 
     2941 ATCAACTTGA CAAAAGTGTT CAGCTGTCAT GGACCCCAGG CGATGACAAC AATAGCCCCA 
     3001 TTACAAAATT CATCATCGAA TATGAAGATG CAATGCACAA GCCAGGGCTG TGGCACCACC 
     3061 AAACTGAAGT TTCTGGAACA CAGACCACAG CCCAGCTGAA GCTGTCTCCT TACGTGAACT 
     3121 ACTCCTTCCG CGTGATGGCA GTGAACAGCA TTGGGAAGAG CTTGCCCAGC GAGGCCTCTG 
     3181 AGCAGTATTT GACGAAAGCC TCAGAACCAG ATAAAAACCC CACAGCTGTG GAAGGACTGG 
     3241 GATCAGAGCC TGATAATTTG GTGATTACGT GGAAGCCCTT GAATGGTTTC GAATCTAATG 
     3301 GGCCAGGCCT TCAGTACAAA GTTAGCTGGC GCCAGAAAGA TGGTGATGAT GAATGGACAT 
     3361 CTGTGGTTGT GGCAAATGTA TCCAAATATA TTGTCTCAGG CACGCCAACC TTTGTTCCAT 
     3421 ACCTGATCAA AGTTCAGGCC CTGAATGACA TGGGGTTTGC CCCCGAGCCA GCTGTAGTCA 
     3481 TGGGACATTC TGGAGAAGAC CTCCCAATGG TGGCTCCTGG GAACGTGCGT GTGAATGTGG 
     3541 TGAACAGTAC CTTAGCCGAG GTGCACTGGG ACCCAGTACC TCTGAAAAGC ATCCGAGGAC 
     3601 ACCTACAAGG CTATCGGATT TACTATTGGA AGACCCAGAG TTCATCTAAA AGAAACAGAC 
     3661 GTCACATTGA GAAAAAGATC CTCACCTTCC AAGGCAGCAA GACTCATGGC ATGTTGCCGG 
     3721 GGCTAGAGCC CTTTAGCCAC TACACACTGA ATGTCCGAGT GGTCAATGGG AAAGGGGAGG 
     3781 GCCCAGCCAG CCCTGACAGA GTCTTTAATA CTCCAGAAGG AGTCCCCAGT GCTCCCTCGT 
     3841 CTTTGAAGAT TGTGAATCCA ACACTGGACT CTCTCACTTT GGAATGGGAT CCACCGAGCC 
     3901 ACCCGAATGG CATTTTGACA GAGTACACCT TAAAGTATCA GCCAATTAAC AGCACACATG 
     3961 AATTAGGCCC TCTGGTAGAT TTGAAAATTC CTGCCAACAA GACACGGTGG ACTTTAAAAA 
     4021 ATTTAAATTT CAGCACTCGA TATAAGTTTT ATTTCTATGC ACAAACATCA GCAGGATCAG 
     4081 GAAGTCAAAT TACAGAGGAA GCAGTAACAA CTGTGGATGA AGCGATGGCA AGCCGGCAGG 
     4141 TGGATATTGC AACTCAGGGC TGGTTCATTG GTCTGATGTG TGCTGTTGCT CTCCTTATCT 
     4201 TAATTTTGCT GATTGTTTGC TTCATCAGAA GAAACAAGGG TGGTAAATAT CCAGTTAAAG 
     4261 AAAAGGAAGA TGCCCATGCT GACCCTGAAA TCCAGCCTAT GAAGGAAGAT GATGGGACAT 
     4321 TTGGAGAATA CAGTGATGCA GAAGACCACA AGCCTTTGAA AAAAGGAAGT CGAACTCCTT 
     4381 CAGACAGGAC TGTGAAAAAA GAAGATAGTG ACGACAGCCT AGTTGACTAT GGAGAAGGGG 
     4441 TTAATGGCCA GTTCAATGAG GATGGCTCCT TTATTGGACA ATACAGTGGT AAGAAAGAGA 
     4501 AAGAGCCGGC TGAAGGAAAC GAAAGCTCAG AGGCACCTTC TCCTGTCAAC GCCATGAATT 
     4561 CCTTTGTTAC GCGTACGCGG CCGCTCGAGC AGAAACTCAT CTCAGAAGAG GATCTGGCAG 
     4621 CAAATGATAT CCTGGATTAC AAGGATGACG ACGATAAGGT TTAAACGGCC GGCCGCGGTC 
     4681 ATAGCTGTTT CCTGAACAGA TCCCGGGTGG CATCCCTGTG ACCCCTCCCC AGTGCCTCTC 
     4741 CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC ACCAGCCTTG TCCTAATAAA ATTAAGTTGC 
     4801 ATCATTTTGT CTGACTAGGT GTCCTTCTAT AATATTATGG GGTGGAGGGG GGTGGTATGG 
     4861 AGCAAGGGGC AAGTTGGGAA GACAACCTGT AGGGCCTGCG GGGTCTATTG GGAACCAAGC 
     4921 TGGAGTGCAG TGGCACAATC TTGGCTCACT GCAATCTCCG CCTCCTGGGT TCAAGCGATT 
     4981 CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA TTCCAGGCAT GCATGACCAG GCTCAGCTAA 
     5041 TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC ACCATATTGG CCAGGCTGGT CTCCAACTCC 
     5101 TAATCTCAGG TGATCTACCC ACCTTGGCCT CCCAAATTGC TGGGATTACA GGCGTGAACC 
     5161 ACTGCTCCCT TCCCTGTCCT TCTGATTTTA AAATAACTAT ACCAGCAGGA GGACGTCCAG 
     5221 ACACAGCATA GGCTACCTGG CCATGCCCAA CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC 
     5281 ACTGTCCTCT CATGCGTTGG GTCCACTCAG TAGATGCCTG TTGAATTGGG TACGCGGCCA 
     5341 GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA 
     5401 TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC 
     5461 CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG 
     5521 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG 
     5581 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT 
     5641 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT 
     5701 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG 
     5761 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT 
     5821 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT 
     5881 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT 
     5941 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC 
     6001 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC 
     6061 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG 
     6121 TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA 
     6181 AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA CCTGAGGCTA TGGCAGGGCC 
     6241 TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG GCCTTCACCC GAACTTGGGG GGTGGGGTGG 
     6301 GGAAAAGGAA GAAACGCGGG CGTATTGGCC CCAATGGGGT CTCGGTGGGG TATCGACAGA 
     6361 GTGCCAGCCC TGGGACCGAA CCCCGCGTTT ATGAACAAAC GACCCAACAC CGTGCGTTTT 
     6421 ATTCTGTCTT TTTATTGCCG TCATAGCGCG GGTTCCTTCC GGTATTGTCT CCTTCCGTGT 
     6481 TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA GAACTCCAGC ATGAGATCCC CGCGCTGGAG 
     6541 GATCATCCAG CCGGCGTCCC GGAAAACGAT TCCGAAGCCC AACCTTTCAT AGAAGGCGGC 
     6601 GGTGGAATCG AAATCTCGTG ATGGCAGGTT GGGCGTCGCT TGGTCGGTCA TTTCGAACCC 
     6661 CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA AGGCGATAGA AGGCGATGCG CTGCGAATCG 
     6721 GGAGCGGCGA TACCGTAAAG CACGAGGAAG CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA 
     6781 GCAATATCAC GGGTAGCCAA CGCTATGTCC TGATAGCGAT CCGCCACACC CAGCCGGCCA 
     6841 CAGTCGATGA ATCCAGAAAA GCGGCCATTT TCCACCATGA TATTCGGCAA GCAGGCATCG 
     6901 CCATGGGTCA CGACGAGATC CTCGCCGTCG GGCATGCTCG CCTTGAGCCT GGCGAACAGT 
     6961 TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG TCCAGATCAT CCTGATCGAC AAGACCGGCT 
     7021 TCCATCCGAG TACGTGCTCG CTCGATGCGA TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA 
     7081 GCCGGATCAA GCGTATGCAG CCGCCGCATT GCATCAGCCA TGATGGATAC TTTCTCGGCA 
     7141 GGAGCAAGGT GAGATGACAG GAGATCCTGC CCCGGCACTT CGCCCAATAG CAGCCAGTCC 
     7201 CTTCCCGCTT CAGTGACAAC GTCGAGCACA GCTGCGCAAG GAACGCCCGT CGTGGCCAGC 
     7261 CACGATAGCC GCGCTGCCTC GTCTTGCAGT TCATTCAGGG CACCGGACAG GTCGGTCTTG 
     7321 ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC AGCCGGAACA CGGCGGCATC AGAGCAGCCG 
     7381 ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT AGCCTCTCCA CCCAAGCGGC CGGAGAACCT 
     7441 GCGTGCAATC CATCTTGTTC AATCATGCGA AACGATCCTC ATCCTGTCTC TTGATCGATC 
     7501 TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC TCCTCACTAC TTCTGGAATA GCTCAGAGGC 
     7561 CGAGGCGGCC TCGGCCTCTG CATAAATAAA AAAAATTAGT CAGCCATGGG GCGGAGAATG 
     7621 GGCGGAACTG GGCGGAGTTA GGGGCGGGAT GGGCGGAGTT AGGGGCGGGA CTATGGTTGC 
     7681 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     7741 ACACCTGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     7801 TGGGGACTTT CCACACCCTA ACTGACACAC ATTCCACAGC TGGTTCTTTC CGCCTCAGGA 
     7861 CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC 
     7921 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA 
     7981 GTGCCACCTG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC 
     8041 AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC 
     8101 TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG 
     8161 TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA 
     8221 CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC 
     8281 TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT 
     8341 TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA 
     8401 CAAAAATTTA ACGCGAATTT T
//