LOCUS       RC230717                9231 bp    DNA     circular  SYN 
DEFINITION  NEO1 (Myc-DDK-tagged)-Human neogenin 1 (NEO1), transcript variant 3
ACCESSION   NM_001172624
VERSION     NM_001172624.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5388..5479 
                     /label="Myc-DDK"  
     misc_feature    5509..6094 
                     /label="PolyA signal"  
     misc_feature    5566..5585 
                     /label="Restriction Enzyme"  
     misc_feature    6370..7042 
                     /label="ColE1"  
     misc_feature    7472..8266 
                     /label="Kan/Neo"  
     misc_feature    8301..8603 
                     /label="SV40 ori"  
     misc_feature    8689..9166 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5370..5376 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5370 
                     /label="NEO1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGGCGGAG CGGGGAGCCC GGCGACTCCT CAGCACCCCC TCCTTCTGGC 
     1081 TCTACTGCCT GCTGCTGCTC GGGCGCCGGG CGCCGGGCGC CGCGGCCGCC AGGAGCGGCT 
     1141 CCGCGCCGCA GTCCCCAGGA GCCAGCATTC GAACGTTCAC TCCATTTTAT TTTCTGGTGG 
     1201 AGCCGGTGGA TACACTCTCA GTTAGAGGCT CTTCTGTTAT ATTAAACTGT TCAGCATATT 
     1261 CTGAGCCTTC TCCAAAAATT GAATGGAAAA AAGATGGAAC TTTTTTAAAC TTAGTATCAG 
     1321 ATGATCGACG CCAGCTTCTC CCGGATGGAT CTTTATTTAT CAGCAATGTG GTGCATTCCA 
     1381 AACACAATAA ACCTGATGAA GGTTATTATC AGTGTGTGGC CACTGTTGAG AGTCTTGGAA 
     1441 CTATTATCAG TAGAACAGCG AAGCTCATAG TAGCAGGTCT TCCAAGATTT ACCAGCCAAC 
     1501 CAGAACCTTC CTCAGTTTAT GCTGGGAACA ATGCAATTCT GAATTGTGAA GTTAATGCAG 
     1561 ATTTGGTCCC ATTTGTGAGG TGGGAACAGA ACAGACAACC CCTTCTTCTG GATGATAGAG 
     1621 TTATCAAACT TCCAAGTGGA ATGCTGGTTA TCAGCAATGC AACTGAAGGA GATGGCGGGC 
     1681 TTTATCGCTG CGTAGTGGAA AGTGGTGGGC CACCAAAGTA TAGTGATGAA GTTGAATTGA 
     1741 AGGTTCTTCC AGATCCTGAG GTGATATCAG ACTTGGTATT TTTGAAACAG CCTTCTCCCT 
     1801 TAGTCAGAGT CATTGGTCAG GATGTAGTGT TGCCATGTGT TGCTTCAGGA CTTCCTACTC 
     1861 CAACCATTAA ATGGATGAAA AATGAGGAGG CACTTGACAC AGAAAGCTCT GAAAGATTGG 
     1921 TATTGCTGGC AGGTGGTAGC CTGGAGATCA GTGATGTTAC TGAGGATGAT GCTGGGACTT 
     1981 ATTTTTGTAT AGCTGATAAT GGAAATGAGA CAATTGAAGC TCAAGCAGAG CTTACAGTGC 
     2041 AAGCTCAACC TGAATTCCTG AAGCAGCCTA CTAATATATA TGCTCACGAA TCTATGGATA 
     2101 TTGTATTTGA ATGTGAAGTG ACTGGAAAAC CAACTCCAAC TGTGAAGTGG GTCAAAAATG 
     2161 GGGATATGGT TATCCCAAGT GATTATTTTA AGATTGTAAA GGAACATAAT CTTCAAGTTT 
     2221 TGGGTCTGGT GAAATCAGAT GAAGGGTTCT ATCAGTGCAT TGCTGAAAAT GATGTTGGAA 
     2281 ATGCACAAGC TGGAGCCCAA CTGATAATCC TTGAACATGC ACCAGCCACA ACGGGACCAC 
     2341 TGCCTTCAGC TCCTCGGGAT GTCGTGGCCT CCCTGGTCTC TACCCGCTTC ATCAAATTGA 
     2401 CGTGGCGGAC ACCTGCATCA GATCCTCACG GAGACAACCT TACCTACTCT GTGTTCTACA 
     2461 CCAAGGAAGG GATTGCTAGG GAACGTGTTG AGAATACCAG TCACCCAGGA GAGATGCAAG 
     2521 TAACCATTCA AAACCTAATG CCAGCGACCG TGTACATCTT TAGAGTTATG GCTCAAAATA 
     2581 AGCATGGCTC AGGAGAGAGT TCAGCTCCAC TGCGAGTAGA AACACAACCT GAGGTTCAGC 
     2641 TCCCTGGCCC AGCACCTAAC CTTCGTGCAT ATGCAGCTTC GCCTACCTCC ATCACTGTTA 
     2701 CGTGGGAAAC ACCAGTGTCT GGCAATGGGG AAATTCAGAA TTATAAATTG TACTACATGG 
     2761 AAAAGGGGAC TGATAAAGAA CAGGATGTTG ATGTTTCAAG TCACTCTTAC ACCATTAATG 
     2821 GGTTGAAAAA ATATACAGAG TATAGTTTCC GAGTGGTGGC CTACAATAAA CATGGTCCTG 
     2881 GAGTTTCCAC ACCAGATGTT GCTGTTCGAA CATTGTCAGA TGTTCCCAGT GCTGCTCCTC 
     2941 AGAATCTGTC CTTGGAAGTG AGAAATTCAA AGAGTATTAT GATTCACTGG CAGCCACCTG 
     3001 CTCCAGCCAC ACAAAATGGG CAGATTACTG GCTACAAGAT TCGCTACCGA AAGGCCTCCC 
     3061 GAAAGAGTGA TGTCACTGAG ACCTTGGTAA GCGGGACACA GCTGTCTCAG CTGATTGAAG 
     3121 GTCTTGATCG GGGGACTGAG TATAATTTCC GAGTGGCTGC TCTAACAATC AATGGTACAG 
     3181 GCCCGGCAAC TGACTGGCTG TCTGCTGAAA CTTTTGAAAG TGACCTAGAT GAAACTCGTG 
     3241 TTCCTGAAGT GCCTAGCTCT CTTCACGTAC GCCCGCTCGT TACTAGCATC GTAGTGAGCT 
     3301 GGACTCCTCC AGAGAATCAG AACATTGTGG TCAGAGGTTA CGCCATTGGT TATGGCATTG 
     3361 GCAGCCCTCA TGCCCAGACC ATCAAAGTGG ACTATAAACA GCGCTATTAC ACCATTGAAA 
     3421 ATCTGGATCC CAGCTCTCAC TATGTGATTA CCCTGAAAGC ATTTAATAAC GTGGGTGAAG 
     3481 GCATCCCCCT GTATGAGAGT GCTGTGACCA GGCCTCACAC AGACACTTCT GAAGTTGATT 
     3541 TATTTGTTAT TAATGCTCCA TACACTCCAG TGCCAGATCC CACTCCCATG ATGCCACCAG 
     3601 TGGGAGTTCA GGCTTCCATT CTGAGTCATG ACACCATCAG GATTACGTGG GCAGACAACT 
     3661 CGCTGCCCAA GCACCAGAAG ATTACAGACT CCCGATACTA CACCGTCCGA TGGAAAACCA 
     3721 ACATCCCAGC AAACACCAAG TACAAGAATG CAAATGCAAC CACTTTGAGT TATTTGGTGA 
     3781 CTGGTTTAAA GCCGAATACA CTCTATGAAT TCTCTGTGAT GGTGACCAAA GGTCGAAGAT 
     3841 CAAGTACATG GAGTATGACA GCCCATGGGA CCACCTTTGA ATTAGTTCCG ACTTCTCCAC 
     3901 CCAAGGATGT GACTGTTGTG AGTAAAGAGG GGAAACCTAA GACCATAATT GTGAATTGGC 
     3961 AGCCTCCCTC CGAAGCCAAT GGCAAAATTA CAGGTTACAT CATATATTAC AGTACAGATG 
     4021 TGAATGCAGA GATACATGAC TGGGTTATTG AGCCTGTTGT GGGAAACAGA CTGACTCACC 
     4081 AGATACAAGA GTTAACTCTT GACACACCAT ACTACTTCAA AATCCAGGCA CGGAACTCAA 
     4141 AGGGCATGGG ACCCATGTCT GAAGCTGTCC AATTCAGAAC ACCTAAAGCC TCAGGGTCTG 
     4201 GAGGGAAAGG AAGCCGGCTG CCAGACCTAG GATCCGACTA CAAACCTCCA ATGAGCGGCA 
     4261 GTAACAGCCC TCATGGGAGC CCCACCTCTC CTCTGGACAG TAATATGCTG CTGGTCATAA 
     4321 TTGTTTCTGT TGGCGTCATC ACCATCGTGG TGGTTGTGAT TATCGCTGTC TTTTGTACCC 
     4381 GTCGTACCAC CTCTCACCAG AAAAAGAAAC GAGCTGCCTG CAAATCAGTG AATGGCTCTC 
     4441 ATAAGTACAA AGGGAATTCC AAAGATGTGA AACCTCCAGA TCTCTGGATC CATCATGAGA 
     4501 GACTGGAGCT GAAACCCATT GATAAGTCTC CAGACCCAAA CCCCATCATG ACTGATACTC 
     4561 CAATTCCTCG CAACTCTCAA GATATCACAC CAGTTGACAA CTCCATGGAC AGCAATATCC 
     4621 ATCAAAGGCG AAATTCATAC AGAGGGCATG AGTCAGAGGA CAGCATGTCT ACACTGGCTG 
     4681 GAAGGCGAGG AATGAGACCA AAAATGATGA TGCCCTTTGA CTCCCAGCCA CCCCAGCCTG 
     4741 TGATTAGTGC CCATCCCATC CATTCCCTCG ATAACCCTCA CCATCATTTC CACTCCAGCA 
     4801 GCCTCGCTTC TCCAGCTCGC AGTCATCTCT ACCACCCGGG CAGCCCATGG CCCATTGGCA 
     4861 CATCCATGTC CCTTTCAGAC AGGGCCAATT CCACAGAATC CGTTCGAAAT ACCCCCAGCA 
     4921 CTGACACCAT GCCAGCCTCT TCGTCTCAAA CATGCTGCAC TGATCACCAG GACCCTGAAG 
     4981 GTGCTACCAG CTCCTCTTAC TTGGCCAGCT CCCAAGAGGA AGATTCAGGC CAGAGTCTTC 
     5041 CCACTGCCCA TGTTCGCCCT TCCCACCCAT TGAAGAGCTT CGCCGTGCCA GCAATCCCGC 
     5101 CTCCAGGACC TCCCACCTAT GATCCTGCAT TGCCAAGCAC ACCATTACTG TCCCAGCAAG 
     5161 CTCTGAACCA TCACATTCAC TCAGTGAAGA CAGCCTCCAT CGGGACTCTA GGAAGGAGCC 
     5221 GGCCTCCTAT GCCAGTGGTT GTTCCCAGTG CCCCTGAAGT GCAGGAGACC ACAAGGATGT 
     5281 TGGAAGACTC CGAGAGTAGC TATGAACCAG ATGAGCTGAC CAAAGAGATG GCCCACCTGG 
     5341 AAGGACTAAT GAAGGACCTA AACGCTATCA CAACAGCAAC GCGTACGCGG CCGCTCGAGC 
     5401 AGAAACTCAT CTCAGAAGAG GATCTGGCAG CAAATGATAT CCTGGATTAC AAGGATGACG 
     5461 ACGATAAGGT TTAAACGGCC GGCCGCGGTC ATAGCTGTTT CCTGAACAGA TCCCGGGTGG 
     5521 CATCCCTGTG ACCCCTCCCC AGTGCCTCTC CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC 
     5581 ACCAGCCTTG TCCTAATAAA ATTAAGTTGC ATCATTTTGT CTGACTAGGT GTCCTTCTAT 
     5641 AATATTATGG GGTGGAGGGG GGTGGTATGG AGCAAGGGGC AAGTTGGGAA GACAACCTGT 
     5701 AGGGCCTGCG GGGTCTATTG GGAACCAAGC TGGAGTGCAG TGGCACAATC TTGGCTCACT 
     5761 GCAATCTCCG CCTCCTGGGT TCAAGCGATT CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA 
     5821 TTCCAGGCAT GCATGACCAG GCTCAGCTAA TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC 
     5881 ACCATATTGG CCAGGCTGGT CTCCAACTCC TAATCTCAGG TGATCTACCC ACCTTGGCCT 
     5941 CCCAAATTGC TGGGATTACA GGCGTGAACC ACTGCTCCCT TCCCTGTCCT TCTGATTTTA 
     6001 AAATAACTAT ACCAGCAGGA GGACGTCCAG ACACAGCATA GGCTACCTGG CCATGCCCAA 
     6061 CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC ACTGTCCTCT CATGCGTTGG GTCCACTCAG 
     6121 TAGATGCCTG TTGAATTGGG TACGCGGCCA GCGGCGAGCG GTATCAGCTC ACTCAAAGGC 
     6181 GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG 
     6241 CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG 
     6301 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG 
     6361 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC 
     6421 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA 
     6481 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT 
     6541 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC 
     6601 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG 
     6661 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC 
     6721 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT 
     6781 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA 
     6841 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG 
     6901 GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA 
     6961 AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT 
     7021 ATATGAGTAA CCTGAGGCTA TGGCAGGGCC TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG 
     7081 GCCTTCACCC GAACTTGGGG GGTGGGGTGG GGAAAAGGAA GAAACGCGGG CGTATTGGCC 
     7141 CCAATGGGGT CTCGGTGGGG TATCGACAGA GTGCCAGCCC TGGGACCGAA CCCCGCGTTT 
     7201 ATGAACAAAC GACCCAACAC CGTGCGTTTT ATTCTGTCTT TTTATTGCCG TCATAGCGCG 
     7261 GGTTCCTTCC GGTATTGTCT CCTTCCGTGT TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA 
     7321 GAACTCCAGC ATGAGATCCC CGCGCTGGAG GATCATCCAG CCGGCGTCCC GGAAAACGAT 
     7381 TCCGAAGCCC AACCTTTCAT AGAAGGCGGC GGTGGAATCG AAATCTCGTG ATGGCAGGTT 
     7441 GGGCGTCGCT TGGTCGGTCA TTTCGAACCC CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA 
     7501 AGGCGATAGA AGGCGATGCG CTGCGAATCG GGAGCGGCGA TACCGTAAAG CACGAGGAAG 
     7561 CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA GCAATATCAC GGGTAGCCAA CGCTATGTCC 
     7621 TGATAGCGAT CCGCCACACC CAGCCGGCCA CAGTCGATGA ATCCAGAAAA GCGGCCATTT 
     7681 TCCACCATGA TATTCGGCAA GCAGGCATCG CCATGGGTCA CGACGAGATC CTCGCCGTCG 
     7741 GGCATGCTCG CCTTGAGCCT GGCGAACAGT TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG 
     7801 TCCAGATCAT CCTGATCGAC AAGACCGGCT TCCATCCGAG TACGTGCTCG CTCGATGCGA 
     7861 TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA GCCGGATCAA GCGTATGCAG CCGCCGCATT 
     7921 GCATCAGCCA TGATGGATAC TTTCTCGGCA GGAGCAAGGT GAGATGACAG GAGATCCTGC 
     7981 CCCGGCACTT CGCCCAATAG CAGCCAGTCC CTTCCCGCTT CAGTGACAAC GTCGAGCACA 
     8041 GCTGCGCAAG GAACGCCCGT CGTGGCCAGC CACGATAGCC GCGCTGCCTC GTCTTGCAGT 
     8101 TCATTCAGGG CACCGGACAG GTCGGTCTTG ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC 
     8161 AGCCGGAACA CGGCGGCATC AGAGCAGCCG ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT 
     8221 AGCCTCTCCA CCCAAGCGGC CGGAGAACCT GCGTGCAATC CATCTTGTTC AATCATGCGA 
     8281 AACGATCCTC ATCCTGTCTC TTGATCGATC TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC 
     8341 TCCTCACTAC TTCTGGAATA GCTCAGAGGC CGAGGCGGCC TCGGCCTCTG CATAAATAAA 
     8401 AAAAATTAGT CAGCCATGGG GCGGAGAATG GGCGGAACTG GGCGGAGTTA GGGGCGGGAT 
     8461 GGGCGGAGTT AGGGGCGGGA CTATGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     8521 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCTGGTT GCTGACTAAT TGAGATGCAT 
     8581 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCCTA ACTGACACAC 
     8641 ATTCCACAGC TGGTTCTTTC CGCCTCAGGA CTCTTCCTTT TTCAATATTA TTGAAGCATT 
     8701 TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA 
     8761 ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGCGCCCTG TAGCGGCGCA 
     8821 TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA 
     8881 GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT 
     8941 CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC 
     9001 CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT 
     9061 TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA 
     9121 ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG 
     9181 GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT T
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