LOCUS       RC230700                8862 bp    DNA     circular  SYN 
DEFINITION  IGSF1 (Myc-DDK-tagged)-Human immunoglobulin superfamily, member 1 (IGSF1), transcript variant 4
ACCESSION   NM_001170962
VERSION     NM_001170962.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5019..5110 
                     /label="Myc-DDK"  
     misc_feature    5140..5725 
                     /label="PolyA signal"  
     misc_feature    5197..5216 
                     /label="Restriction Enzyme"  
     misc_feature    6001..6673 
                     /label="ColE1"  
     misc_feature    7103..7897 
                     /label="Kan/Neo"  
     misc_feature    7932..8234 
                     /label="SV40 ori"  
     misc_feature    8320..8797 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5001..5007 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5001 
                     /label="IGSF1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GACCCTGGAC AGACCAGGGG AGGGGGCCAC CATGCTGAAG ACATTCACTG 
     1081 TTTTGCTCTT TTGCATTCTG ATGGACCCTC AACCGGAGTT GTGGATAGAG TCCAACTACC 
     1141 CCCAGGCCCC TTGGGAGAAC ATCACGCTTT GGTGCCGAAG CCCCTCTCGG ATATCAAGCA 
     1201 AGTTCCTGCT GCTGAAGGAT AAGACACAGA TGACCTGGAT CCGCCCTTCC CACAAGACCT 
     1261 TCCAAGTTTC ATTCCTTATA GGTGCCCTTA CTGAGTCCAA TGCAGGTCTT TACCGGTGCT 
     1321 GCTACTGGAA GGAGACAGGC TGGTCAAAGC CCAGTAAAGT TCTAGAGTTG GAGGCACCAG 
     1381 GCCAACTGCC CAAGCCCATC TTCTGGATTC AGGCTGAGAC CCCCGCTCTT CCTGGGTGTA 
     1441 ATGTTAACAT CCTCTGCCAT GGCTGGCTGC AGGATTTGGT ATTCATGCTG TTTAAAGAGG 
     1501 GATATGCAGA GCCTGTGGAT TACCAAGTCC CAACTGGGAC AATGGCCATA TTCTCCATTG 
     1561 ACAACCTGAC ACCTGAGGAT GAAGGGGTTT ACATCTGCCG CACTCATATC CAGATGCTCC 
     1621 CCACCCTGTG GTCAGAGCCC AGCAACCCCC TGAAGCTGGT TGTAGCAGGA CTCTACCCCA 
     1681 AACCAACTTT GACAGCCCAT CCTGGGCCCA TCATGGCACC TGGAGAAAGC CTGAATCTCA 
     1741 GGTGCCAAGG GCCAATCTAT GGAATGACCT TTGCTCTAAT GAGGGTTGAA GACTTGGAGA 
     1801 AGTCCTTTTA CCACAAGAAG ACAATAAAAA ATGAGGCAAA TTTCTTCTTC CAGTCTTTGA 
     1861 AGATCCAAGA TACTGGACAT TACCTCTGTT TTTACTATGA CGCATCATAT AGAGGTTCAC 
     1921 TCCTTAGTGA TGTCCTGAAA ATCTGGGTAA CTGACACTTT CCCCAAGACC TGGCTACTTG 
     1981 CTCGGCCCAG TGCTGTGGTC CAAATGGGTC AGAATGTGAG CCTACGGTGT CGAGGACCAG 
     2041 TGGATGGAGT GGGTCTTGCA CTCTATAAGA AAGGAGAAGA CAAACCACTT CAATTTTTGG 
     2101 ATGCCACCAG CATCGATGAC AACACATCAT TCTTCCTCAA CAATGTAACC TACAGTGATA 
     2161 CTGGCATCTA TAGCTGCCAC TATCTTCTCA CCTGGAAGAC CTCCATTAGG ATGCCATCAC 
     2221 ACAACACTGT GGAGCTTATG GTTGTAGATA AGCCCCCCAA ACCCTCCCTG TCAGCTTGGC 
     2281 CAAGCACTGT GTTCAAGCTA GGAAAGGCCA TCACCCTTCA GTGCCGAGTA TCTCATCCAG 
     2341 TACTGGAATT TTCTCTGGAA TGGGAAGAAA GAGAAACATT CCAAAAATTC TCAGTAAACG 
     2401 GAGACTTCAT CATCAGTAAT GTTGACGGGA AAGGCACAGG GACCTACAGT TGCAGCTATC 
     2461 GCGTAGAGAC ACATCCTAAC ATCTGGTCAC ATCGCAGTGA GCCCCTGAAG CTGATGGGGC 
     2521 CAGCAGGCTA TCTCACCTGG AATTACGTTC TGAATGAAGC TATCAGGTTG TCTCTAATCA 
     2581 TGCAGCTTGT TGCCTTGCTG TTGGTAGTGC TGTGGATAAG GTGGAAGTGT CGGAGACTCA 
     2641 GAATCAGAGA AGCCTGGTTG CTGGGAACAG CTCAAGGGGT CACCATGCTC TTCATAGTCA 
     2701 CGGCCCTTCT CTGCTGTGGA CTGTGCAATG GGGTATTGAT AGAAGAGACT GAAATAGTCA 
     2761 TGCCAACCCC TAAGCCTGAG CTGTGGGCAG AGACCAACTT TCCTCTGGCC CCGTGGAAGA 
     2821 ACTTAACCCT CTGGTGCAGA AGCCCTTCTG GCTCAACTAA GGAGTTTGTG TTGCTGAAGG 
     2881 ATGGGACCGG GTGGATCGCC ACTCGCCCGG CCTCAGAGCA GGTCCGGGCT GCCTTCCCCC 
     2941 TTGGCGCCCT GACCCAGAGC CACACCGGGA GCTACCACTG CCATTCATGG GAGGAGATGG 
     3001 CTGTATCGGA GCCCAGTGAG GCACTTGAGC TGGTGGGGAC AGACATCCTC CCCAAACCTG 
     3061 TCATTTCTGC TTCCCCCACA ATCCGGGGCC AGGAACTACA ACTCCGGTGC AAAGGATGGC 
     3121 TGGCAGGCAT GGGGTTTGCT CTGTATAAGG AGGGAGAGCA AGAACCTGTC CAGCAACTTG 
     3181 GTGCTGTTGG AAGAGAAGCC TTCTTTACAA TCCAGAGAAT GGAGGATAAA GACGAAGGCA 
     3241 ATTACAGCTG CCGCACTCAC ACTGAAAAAC GCCCCTTCAA GTGGTCTGAG CCCAGTGAGC 
     3301 CGCTGGAGCT TGTCATAAAA GAAATGTACC CTAAGCCCTT CTTCAAGACA TGGGCCAGCC 
     3361 CTGTGGTCAC CCCTGGTGCC CGAGTGACTT TCAATTGCTC CACCCCCCAC CAGCATATGA 
     3421 GCTTTATTCT TTACAAAGAT GGAAGTGAAA TAGCATCCAG TGACAGGTCC TGGGCAAGTC 
     3481 CGGGGGCCAG TGCAGCTCAC TTTCTAATCA TTTCGGTGGG CATTGGTGAT GGAGGGAATT 
     3541 ACAGCTGCCG ATATTACGAC TTTTCTATCT GGTCTGAGCC CAGCGACCCT GTGGAGCTCG 
     3601 TGGTGACAGA ATTCTACCCC AAACCCACTC TCCTGGCACA GCCAGGTCCT GTGGTGTTTC 
     3661 CTGGGAAGAG TGTGATCCTG CGCTGCCAAG GGACTTTCCA GGGCATGAGG TTCGCCCTCT 
     3721 TGCAGGAGGG AGCCCATGTT CCCTTACAGT TTCGGAGTGT CTCAGGGAAC TCAGCTGACT 
     3781 TCCTTCTCCA CACTGTTGGA GCAGAGGACT CTGGGAACTA TAGCTGTATC TACTATGAGA 
     3841 CAACCATGTC AAACAGGGGG TCATATCTCA GTATGCCCCT TATGATCTGG GTGACTGACA 
     3901 CATTCCCTAA GCCATGGTTG TTTGCTGAGC CCAGTTCTGT GGTTCCCATG GGGCAGAATG 
     3961 TTACTCTCTG GTGCCGAGGG CCGGTCCATG GAGTAGGATA CATTCTGCAC AAAGAAGGAG 
     4021 AAGCCACTTC AATGCAGCTC TGGGGATCCA CCAGTAATGA CGGGGCATTC CCCATCACCA 
     4081 ATATATCTGG TACTAGCATG GGGCGTTACA GCTGCTGCTA CCACCCTGAC TGGACCAGTT 
     4141 CTATCAAGAT ACAACCTAGC AACACCCTGG AACTCCTAGT CACAGGCTTA CTCCCCAAAC 
     4201 CCAGCCTATT AGCCCAGCCT GGTCCCATGG TGGCCCCTGG CGAAAATATG ACTCTTCAGT 
     4261 GTCAAGGGGA ACTGCCAGAC TCAACATTTG TCCTGTTGAA GGAGGGGGCT CAGGAGCCTT 
     4321 TAGAGCAACA GAGGCCAAGT GGGTACAGGG CTGACTTCTG GATGCCAGCA GTGAGAGGTG 
     4381 AAGACTCTGG GATCTATAGC TGTGTTTATT ATTTGGACTC TACTCCCTTT GCAGCTTCAA 
     4441 ATCACAGTGA CTCCCTGGAG ATCTGGGTGA CTGATAAGCC CCCTAAACCC TCTCTGTCAG 
     4501 CCTGGCCCAG CACCATGTTC AAGTTAGGGA AGGACATCAC CCTTCAGTGC CGAGGACCCC 
     4561 TGCCAGGTGT TGAATTTGTC CTAGAACATG ATGGAGAAGA AGCACCTCAG CAGTTTTCAG 
     4621 AGGATGGAGA CTTTGTCATC AACAACGTAG AAGGAAAAGG CATTGGAAAC TACAGCTGCA 
     4681 GCTACCGCCT CCAGGCCTAC CCTGATATCT GGTCAGAGCC TAGTGATCCC CTGGAGCTGG 
     4741 TGGGGGCAGC AGGGCCTGTT GCTCAGGAGT GCACTGTAGG GAACATTGTC CGAAGTAGCC 
     4801 TAATCGTGGT GGTTGTTGTA GCCTTGGGGG TAGTGCTAGC CATAGAGTGG AAGAAGTGGC 
     4861 CTCGACTGCG AACCAGAGGC TCAGAGACAG ACGGAAGAGA CCAGACCATT GCCCTTGAAG 
     4921 AGTGTAACCA AGAAGGAGAA CCAGGCACCC CTGCCAATTC TCCTTCATCA ACCTCTCAGA 
     4981 GAATCTCTGT GGAACTGCCC GTTCCAATAA CGCGTACGCG GCCGCTCGAG CAGAAACTCA 
     5041 TCTCAGAAGA GGATCTGGCA GCAAATGATA TCCTGGATTA CAAGGATGAC GACGATAAGG 
     5101 TTTAAACGGC CGGCCGCGGT CATAGCTGTT TCCTGAACAG ATCCCGGGTG GCATCCCTGT 
     5161 GACCCCTCCC CAGTGCCTCT CCTGGCCCTG GAAGTTGCCA CTCCAGTGCC CACCAGCCTT 
     5221 GTCCTAATAA AATTAAGTTG CATCATTTTG TCTGACTAGG TGTCCTTCTA TAATATTATG 
     5281 GGGTGGAGGG GGGTGGTATG GAGCAAGGGG CAAGTTGGGA AGACAACCTG TAGGGCCTGC 
     5341 GGGGTCTATT GGGAACCAAG CTGGAGTGCA GTGGCACAAT CTTGGCTCAC TGCAATCTCC 
     5401 GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA 
     5461 TGCATGACCA GGCTCAGCTA ATTTTTGTTT TTTTGGTAGA GACGGGGTTT CACCATATTG 
     5521 GCCAGGCTGG TCTCCAACTC CTAATCTCAG GTGATCTACC CACCTTGGCC TCCCAAATTG 
     5581 CTGGGATTAC AGGCGTGAAC CACTGCTCCC TTCCCTGTCC TTCTGATTTT AAAATAACTA 
     5641 TACCAGCAGG AGGACGTCCA GACACAGCAT AGGCTACCTG GCCATGCCCA ACCGGTGGGA 
     5701 CATTTGAGTT GCTTGCTTGG CACTGTCCTC TCATGCGTTG GGTCCACTCA GTAGATGCCT 
     5761 GTTGAATTGG GTACGCGGCC AGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG 
     5821 GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA 
     5881 GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA 
     5941 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG 
     6001 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT 
     6061 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG 
     6121 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC 
     6181 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT 
     6241 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA 
     6301 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC 
     6361 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC 
     6421 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT 
     6481 TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC 
     6541 TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT 
     6601 CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA 
     6661 ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC GACGTTGGCT GCGAGCCCTG GGCCTTCACC 
     6721 CGAACTTGGG GGGTGGGGTG GGGAAAAGGA AGAAACGCGG GCGTATTGGC CCCAATGGGG 
     6781 TCTCGGTGGG GTATCGACAG AGTGCCAGCC CTGGGACCGA ACCCCGCGTT TATGAACAAA 
     6841 CGACCCAACA CCGTGCGTTT TATTCTGTCT TTTTATTGCC GTCATAGCGC GGGTTCCTTC 
     6901 CGGTATTGTC TCCTTCCGTG TTTCAGTTAG CCTCCCCCTA GGGTGGGCGA AGAACTCCAG 
     6961 CATGAGATCC CCGCGCTGGA GGATCATCCA GCCGGCGTCC CGGAAAACGA TTCCGAAGCC 
     7021 CAACCTTTCA TAGAAGGCGG CGGTGGAATC GAAATCTCGT GATGGCAGGT TGGGCGTCGC 
     7081 TTGGTCGGTC ATTTCGAACC CCAGAGTCCC GCTCAGAAGA ACTCGTCAAG AAGGCGATAG 
     7141 AAGGCGATGC GCTGCGAATC GGGAGCGGCG ATACCGTAAA GCACGAGGAA GCGGTCAGCC 
     7201 CATTCGCCGC CAAGCTCTTC AGCAATATCA CGGGTAGCCA ACGCTATGTC CTGATAGCGA 
     7261 TCCGCCACAC CCAGCCGGCC ACAGTCGATG AATCCAGAAA AGCGGCCATT TTCCACCATG 
     7321 ATATTCGGCA AGCAGGCATC GCCATGGGTC ACGACGAGAT CCTCGCCGTC GGGCATGCTC 
     7381 GCCTTGAGCC TGGCGAACAG TTCGGCTGGC GCGAGCCCCT GATGCTCTTC GTCCAGATCA 
     7441 TCCTGATCGA CAAGACCGGC TTCCATCCGA GTACGTGCTC GCTCGATGCG ATGTTTCGCT 
     7501 TGGTGGTCGA ATGGGCAGGT AGCCGGATCA AGCGTATGCA GCCGCCGCAT TGCATCAGCC 
     7561 ATGATGGATA CTTTCTCGGC AGGAGCAAGG TGAGATGACA GGAGATCCTG CCCCGGCACT 
     7621 TCGCCCAATA GCAGCCAGTC CCTTCCCGCT TCAGTGACAA CGTCGAGCAC AGCTGCGCAA 
     7681 GGAACGCCCG TCGTGGCCAG CCACGATAGC CGCGCTGCCT CGTCTTGCAG TTCATTCAGG 
     7741 GCACCGGACA GGTCGGTCTT GACAAAAAGA ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC 
     7801 ACGGCGGCAT CAGAGCAGCC GATTGTCTGT TGTGCCCAGT CATAGCCGAA TAGCCTCTCC 
     7861 ACCCAAGCGG CCGGAGAACC TGCGTGCAAT CCATCTTGTT CAATCATGCG AAACGATCCT 
     7921 CATCCTGTCT CTTGATCGAT CTTTGCAAAA GCCTAGGCCT CCAAAAAAGC CTCCTCACTA 
     7981 CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC CTCGGCCTCT GCATAAATAA AAAAAATTAG 
     8041 TCAGCCATGG GGCGGAGAAT GGGCGGAACT GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT 
     8101 TAGGGGCGGG ACTATGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     8161 TGGGGAGCCT GGGGACTTTC CACACCTGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     8221 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCCT AACTGACACA CATTCCACAG 
     8281 CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT 
     8341 TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT 
     8401 CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGCGCCCT GTAGCGGCGC ATTAAGCGCG 
     8461 GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT 
     8521 CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA 
     8581 AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA 
     8641 CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT 
     8701 TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC 
     8761 AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG 
     8821 TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TT
//