LOCUS       RC230684                8658 bp    DNA     circular  SYN 
DEFINITION  SYNJ2 (Myc-DDK-tagged)-Human synaptojanin 2 (SYNJ2), transcript variant 2
ACCESSION   NM_001178088
VERSION     NM_001178088.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4815..4906 
                     /label="Myc-DDK"  
     misc_feature    4936..5521 
                     /label="PolyA signal"  
     misc_feature    4993..5012 
                     /label="Restriction Enzyme"  
     misc_feature    5797..6469 
                     /label="ColE1"  
     misc_feature    6899..7693 
                     /label="Kan/Neo"  
     misc_feature    7728..8030 
                     /label="SV40 ori"  
     misc_feature    8116..8593 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4797..4803 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4797 
                     /label="SYNJ2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GATTTACATG GACGATGGAG TGTCATCTTT TGTCCAGATC AGAGGCTCCG 
     1081 TTCCGCTGTT CTGGGAACAG CCAGGGCTTC AGGTTGGCTC CCATCATCTG AGACTCCACA 
     1141 GAGGCCTGGA AGCCAATGCC CCTGCTTTCG ACAGGCACAT GGTGCTTCTG AAGGAGCAGT 
     1201 ACGGGCAGCA GGTGGTCGTG AACCTTCTGG GAAGCAGAGG CGGAGAGGAG GTGCTCAACA 
     1261 GAGCCTTCAA GAAGCTGCTC TGGGCTTCTT GCCACGCGGG CGACACGCCT ATGATCAATT 
     1321 TTGACTTCCA TCAGTTTGCC AAAGGTGGGA AGCTAGAGAA ATTGGAGACC CTCTTGAGGC 
     1381 CACAGTTAAA GCTGCACTGG GAAGACTTCG ATGTGTTCAC AAAGGGGGAG AACGTCAGTC 
     1441 CACGTTTTCA GAAAGGCACT TTGCGGATGA ACTGTCTTGA CTGCCTGGAC CGAACCAACA 
     1501 CTGTGCAGAG CTTCATCGCG CTCGAGGTCC TGCATCTGCA GCTCAAGACC CTGGGGCTGA 
     1561 GTTCAAAACC CATCGTTGAC CGCTTTGTGG AGTCCTTCAA AGCCATGTGG TCTCTGAATG 
     1621 GCCACAGCCT GAGCAAGGTG TTCACAGGCA GCAGAGCCCT GGAAGGGAAG GCCAAGGTGG 
     1681 GGAAGCTGAA GGATGGAGCC CGGTCCATGT CTCGAACCAT CCAGTCCAAC TTCTTCGACG 
     1741 GGGTGAAGCA GGAGGCCATC AAGCTGCTGC TGGTTGGGGA CGTCTACGGC GAGGAGGTGG 
     1801 CAGACAAAGG GGGCATGCTG CTGGACAGCA CGGCGCTCCT GGTGACTCCC AGGATCCTGA 
     1861 AAGCTATGAC TGAGCGTCAG TCCGAATTCA CAAATTTCAA GCGGATCCGG ATTGCTATGG 
     1921 GGACCTGGAA CGTGAACGGA GGAAAGCAGT TCCGGAGCAA CGTGCTCAGG ACGGCGGAGC 
     1981 TGACAGACTG GCTGCTCGAC TCGCCCCAGC TCTCGGGAGC TACCGACTCC CAGGATGACA 
     2041 GCAGCCCAGC TGACATATTT GCTGTGGGGT TTGAAGAGAT GGTGGAATTG AGCGCAGGGA 
     2101 ATATTGTCAA TGCCAGTACT ACCAACAAGA AGATGTGGGG TGAACAGCTT CAGAAAGCCA 
     2161 TCTCACGCTC TCATAGATAC ATTCTGTTGA CTTCGGCACA GCTGGTGGGC GTCTGTCTTT 
     2221 ATATCTTTGT ACGTCCATAC CATGTCCCGT TCATCAGGGA CGTAGCCATC GACACAGTGA 
     2281 AGACGGGCAT GGGGGGCAAG GCGGGGAACA AGGGCGCCGT CGGCATCCGC TTCCAGTTCC 
     2341 ACAGCACCAG CTTCTGCTTC ATATGTAGTC ACCTGACGGC CGGGCAGTCC CAGGTGAAGG 
     2401 AGCGGAATGA AGACTACAAG GAGATCACCC AGAAACTCTG CTTCCCAATG GGGAGAAATG 
     2461 TTTTTTCTCA TGATTATGTA TTTTGGTGTG GCGATTTCAA CTACCGCATT GATCTTACTT 
     2521 ATGAAGAAGT CTTCTATTTT GTTAAACGCC AAGACTGGAA GAAACTTCTG GAATTTGATC 
     2581 AACTACAGCT ACAGAAATCA AGTGGAAAAA TTTTTAAGGA CTTTCACGAA GGAGCCATTA 
     2641 ACTTTGGACC CACCTACAAG TATGACGTTG GCTCAGCCGC CTACGATACA AGCGACAAAT 
     2701 GCCGCACCCC CGCCTGGACA GACAGGGTGC TGTGGTGGAG GAAGAAACAT CCCTTTGATA 
     2761 AAACAGCTGG AGAACTCAAC CTTCTAGACA GTGATCTAGA TGTTGACACC AAAGTCAGAC 
     2821 ACACCTGGTC TCCTGGTGCC CTGCAGTATT ATGGTCGTGC GGAGCTACAA GCGTCTGATC 
     2881 ACAGACCTGT GCTGGCGATC GTGGAGGTGG AAGTTCAGGA AGTCGATGTG GGTGCTCGGG 
     2941 AGAGGGTTTT CCAGGAAGTG TCCTCCTTCC AGGGCCCCCT GGATGCCACT GTTGTAGTAA 
     3001 ACCTTCAATC ACCGACCTTA GAAGAGAAAA ACGAGTTTCC AGAGGACCTG CGTACTGAGC 
     3061 TCATGCAGAC CTTGGGGAGT TATGGGACAA TTGTTCTTGT CAGGATCAAC CAAGGGCAGA 
     3121 TGCTGGTAAC TTTTGCAGAC AGTCACTCGG CTCTCAGTGT CCTGGACGTG GACGGTATGA 
     3181 AGGTGAAAGG CAGAGCAGTG AAGATTAGAC CGAAGACCAA GGACTGGCTG AAAGGTTTGC 
     3241 GAGAGGAGAT CATTCGGAAA CGAGACAGCA TGGCCCCCGT GTCTCCCACT GCCAACTCCT 
     3301 GTTTGCTGGA GGAAAACTTT GACTTCACAA GTTTGGACTA TGAGTCAGAA GGGGATATTC 
     3361 TTGAAGACGA TGAAGACTAC TTGGTGGATG AATTCAATCA GCCTGGAGTC TCGGACAGTG 
     3421 AACTCGGGGG AGACGACCTC TCTGATGTCC CCGGCCCCAC AGCACTGGCT CCTCCCAGCA 
     3481 AGTCACCTGC TCTCACCAAA AAGAAGCAGC ATCCAACGTA CAAAGATGAC GCGGACCTGG 
     3541 TGGAGCTCAA GCGGGAGCTG GAAGCCGTCG GGGAGTTCCG CCACCGTTCT CCGAGCAGGT 
     3601 CTCTGTCGGT CCCCAACCGG CCTCGGCCAC CTCAACCCCC GCAGAGACCC CCCCCTCCAA 
     3661 CCGGTTTAAT GGTGAAAAAG TCGGCTTCAG ATGCGTCCAT CTCCTCCGGC ACCCATGGAC 
     3721 AGTATTCAAT TTTGCAGACG GCAAGACTTC TACCAGGAGC ACCTCAGCAA CCTCCCAAGG 
     3781 CTCGGACTGG AATAAGTAAA CCTTATAATG TCAAGCAGAT CAAAACCACC AATGCCCAGG 
     3841 AGGCAGAAGC AGCAATCCGG TGTCTCCTGG AAGCCAGAGG AGGTGCCTCC GAAGAAGCCC 
     3901 TAAGTGCCGT GGCCCCAAGG GACCTTGAAG CATCCTCTGA ACCAGAGCCC ACACCGGGGG 
     3961 CAGCCAAACC AGAGACCCCA CAGGCGCCCC CACTCCTTCC CCGTCGGCCC CCACCCAGAG 
     4021 TTCCTGCCAT CAAGAAGCCA ACCTTGAGAA GGACAGGAAA GCCCCTGTCA CCGGAAGAAC 
     4081 AGTTTGAGCA ACAGACTGTC CATTTTACAA TCGGGCCCCC GGAGACAAGC GTTGAGGCCC 
     4141 CTCCTGTCGT GACAGCCCCT CGAGTCCCTC CTGTTCCCAA ACCAAGAACA TTTCAGCCTG 
     4201 GGAAAGCTGC AGAGAGGCCA AGCCACAGGA AGCCAGCATC AGACGAAGCC CCTCCTGGGG 
     4261 CAGGAGCCTC TGTGCCACCA CCTCTGGAGG CGCCGCCTCT TGTGCCCAAG GTACCCCCGA 
     4321 GGAGGAAGAA GTCAGCCCCC GCAGCCTTCC ACCTGCAGGT CCTGCAGAGC AACAGCCAGC 
     4381 TTCTCCAGGG CCTCACTTAC AATAGCAGTG ACAGCCCCTC TGGGCACCCA CCTGCCGCGG 
     4441 GCACCGTCTT CCCACAAGGG GACTTTCTCA GCACTTCATC TGCTACAAGC CCCGACAGCG 
     4501 ATGGCACCAA AGCGATGAAG CCAGAGGCAG CCCCACTTCT TGGTGATTAT CAGGACCCCT 
     4561 TCTGGAACCT TCTTCACCAC CCTAAACTGT TGAATAACAC TTGGCTTTCT AAGAGCTCAG 
     4621 ACCCTTTGGA CTCAGGAACC AGGAGCCCCA AAAGAGATCC CATAGACCCA GTGTCAGCTG 
     4681 GCGCTTCAGC TGCCAAGGCA GAGCTGCCAC CAGATCATGA ACACAAAACC TTAGGTCACT 
     4741 GGGTGACAAT CAGTGACCAA GAAAAGAGGA CAGCACTGCA GGTGTTTGAC CCACTGGCAA 
     4801 AAACAACGCG TACGCGGCCG CTCGAGCAGA AACTCATCTC AGAAGAGGAT CTGGCAGCAA 
     4861 ATGATATCCT GGATTACAAG GATGACGACG ATAAGGTTTA AACGGCCGGC CGCGGTCATA 
     4921 GCTGTTTCCT GAACAGATCC CGGGTGGCAT CCCTGTGACC CCTCCCCAGT GCCTCTCCTG 
     4981 GCCCTGGAAG TTGCCACTCC AGTGCCCACC AGCCTTGTCC TAATAAAATT AAGTTGCATC 
     5041 ATTTTGTCTG ACTAGGTGTC CTTCTATAAT ATTATGGGGT GGAGGGGGGT GGTATGGAGC 
     5101 AAGGGGCAAG TTGGGAAGAC AACCTGTAGG GCCTGCGGGG TCTATTGGGA ACCAAGCTGG 
     5161 AGTGCAGTGG CACAATCTTG GCTCACTGCA ATCTCCGCCT CCTGGGTTCA AGCGATTCTC 
     5221 CTGCCTCAGC CTCCCGAGTT GTTGGGATTC CAGGCATGCA TGACCAGGCT CAGCTAATTT 
     5281 TTGTTTTTTT GGTAGAGACG GGGTTTCACC ATATTGGCCA GGCTGGTCTC CAACTCCTAA 
     5341 TCTCAGGTGA TCTACCCACC TTGGCCTCCC AAATTGCTGG GATTACAGGC GTGAACCACT 
     5401 GCTCCCTTCC CTGTCCTTCT GATTTTAAAA TAACTATACC AGCAGGAGGA CGTCCAGACA 
     5461 CAGCATAGGC TACCTGGCCA TGCCCAACCG GTGGGACATT TGAGTTGCTT GCTTGGCACT 
     5521 GTCCTCTCAT GCGTTGGGTC CACTCAGTAG ATGCCTGTTG AATTGGGTAC GCGGCCAGCG 
     5581 GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA 
     5641 CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC 
     5701 GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC 
     5761 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG 
     5821 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT 
     5881 CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA 
     5941 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC 
     6001 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC 
     6061 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT 
     6121 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT 
     6181 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC 
     6241 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA 
     6301 AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 
     6361 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 
     6421 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAACCT GAGGCTATGG CAGGGCCTGC 
     6481 CGCCCCGACG TTGGCTGCGA GCCCTGGGCC TTCACCCGAA CTTGGGGGGT GGGGTGGGGA 
     6541 AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA ATGGGGTCTC GGTGGGGTAT CGACAGAGTG 
     6601 CCAGCCCTGG GACCGAACCC CGCGTTTATG AACAAACGAC CCAACACCGT GCGTTTTATT 
     6661 CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC 
     6721 AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA CTCCAGCATG AGATCCCCGC GCTGGAGGAT 
     6781 CATCCAGCCG GCGTCCCGGA AAACGATTCC GAAGCCCAAC CTTTCATAGA AGGCGGCGGT 
     6841 GGAATCGAAA TCTCGTGATG GCAGGTTGGG CGTCGCTTGG TCGGTCATTT CGAACCCCAG 
     6901 AGTCCCGCTC AGAAGAACTC GTCAAGAAGG CGATAGAAGG CGATGCGCTG CGAATCGGGA 
     6961 GCGGCGATAC CGTAAAGCAC GAGGAAGCGG TCAGCCCATT CGCCGCCAAG CTCTTCAGCA 
     7021 ATATCACGGG TAGCCAACGC TATGTCCTGA TAGCGATCCG CCACACCCAG CCGGCCACAG 
     7081 TCGATGAATC CAGAAAAGCG GCCATTTTCC ACCATGATAT TCGGCAAGCA GGCATCGCCA 
     7141 TGGGTCACGA CGAGATCCTC GCCGTCGGGC ATGCTCGCCT TGAGCCTGGC GAACAGTTCG 
     7201 GCTGGCGCGA GCCCCTGATG CTCTTCGTCC AGATCATCCT GATCGACAAG ACCGGCTTCC 
     7261 ATCCGAGTAC GTGCTCGCTC GATGCGATGT TTCGCTTGGT GGTCGAATGG GCAGGTAGCC 
     7321 GGATCAAGCG TATGCAGCCG CCGCATTGCA TCAGCCATGA TGGATACTTT CTCGGCAGGA 
     7381 GCAAGGTGAG ATGACAGGAG ATCCTGCCCC GGCACTTCGC CCAATAGCAG CCAGTCCCTT 
     7441 CCCGCTTCAG TGACAACGTC GAGCACAGCT GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC 
     7501 GATAGCCGCG CTGCCTCGTC TTGCAGTTCA TTCAGGGCAC CGGACAGGTC GGTCTTGACA 
     7561 AAAAGAACCG GGCGCCCCTG CGCTGACAGC CGGAACACGG CGGCATCAGA GCAGCCGATT 
     7621 GTCTGTTGTG CCCAGTCATA GCCGAATAGC CTCTCCACCC AAGCGGCCGG AGAACCTGCG 
     7681 TGCAATCCAT CTTGTTCAAT CATGCGAAAC GATCCTCATC CTGTCTCTTG ATCGATCTTT 
     7741 GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC TCACTACTTC TGGAATAGCT CAGAGGCCGA 
     7801 GGCGGCCTCG GCCTCTGCAT AAATAAAAAA AATTAGTCAG CCATGGGGCG GAGAATGGGC 
     7861 GGAACTGGGC GGAGTTAGGG GCGGGATGGG CGGAGTTAGG GGCGGGACTA TGGTTGCTGA 
     7921 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     7981 CCTGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     8041 GGACTTTCCA CACCCTAACT GACACACATT CCACAGCTGG TTCTTTCCGC CTCAGGACTC 
     8101 TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA 
     8161 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG 
     8221 CCACCTGACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC 
     8281 GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT 
     8341 CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC 
     8401 CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT 
     8461 AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT 
     8521 AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT 
     8581 GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA 
     8641 AAATTTAACG CGAATTTT
//