LOCUS       RC230668                8382 bp    DNA     circular  SYN 
DEFINITION  PLCB3 (Myc-DDK-tagged)-Human phospholipase C, beta 3 (phosphatidylinositol-specific) (PLCB3), transcript variant 2
ACCESSION   NM_001184883
VERSION     NM_001184883.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4539..4630 
                     /label="Myc-DDK"  
     misc_feature    4660..5245 
                     /label="PolyA signal"  
     misc_feature    4717..4736 
                     /label="Restriction Enzyme"  
     misc_feature    5521..6193 
                     /label="ColE1"  
     misc_feature    6623..7417 
                     /label="Kan/Neo"  
     misc_feature    7452..7754 
                     /label="SV40 ori"  
     misc_feature    7840..8317 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4521..4527 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4521 
                     /label="PLCB3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGGGCGCC CAGCCCGGCG TCCACGCGCT GCAGTTGGAG CCGCCCACCG 
     1081 TGGTGGAGAC CCTGCGGCGC GGGAGTAAGT TCATCAAATG GGACGAGGAG AAGCTGATGA 
     1141 CGGTGGTGTC TGGGCCAGAC CCAGTGAACA CAGTGTTCTT GAACTTCATG GCCGTGCAGG 
     1201 ATGACACAGC CAAGGTCTGG TCTGAGGAGC TATTCAAGCT GGCTATGAAC ATCCTGGCTC 
     1261 AGAACGCCTC CCGGAACACC TTCCTGCGCA AAGCATACAC GAAGCTGAAG CTGCAGGTGA 
     1321 ACCAGGATGG TCGGATCCCC GTCAAGAACA TCCTGAAGAT GTTCTCAGCA GACAAGAAGC 
     1381 GGGTGGAGAC TGCGCTGGAA TCCTGTGGCC TCAAATTCAA CCGGAGTGAG TCCATCCGGC 
     1441 CTGATGAGTT TTCCTTGGAA ATCTTTGAGC GGTTCCTGAA CAAGCTGTGT CTGCGGCCGG 
     1501 ACATTGACAA GATCCTGCTG GAGATAGGCG CCAAGGGCAA GCCATACCTG ACGCTGGAGC 
     1561 AGCTCATGGA CTTCATCAAC CAGAAGCAAC GCGACCCGAG ACTCAACGAA GTGCTGTACC 
     1621 CGCCCCTGCG GCCCTCCCAG GCCCGGCTGC TCATCGAAAA GTATGAGCCC AACCAGCAGT 
     1681 TTCTGGAGCG AGACCAGATG TCCATGGAGG GCTTTAGCCG CTACCTGGGA GGCGAGGAGA 
     1741 ATGGCATCCT GCCCCTGGAA GCCCTGGATC TGAGCACGGA CATGACCCAG CCACTGAGTG 
     1801 CCTACTTCAT CAACTCCTCG CATAACACCT ATCTCACTGC GGGGCAGCTG GCTGGGACCT 
     1861 CGTCGGTGGA GATGTACCGC CAGGCACTAC TATGGGGCTG CCGCTGCGTG GAGCTGGACG 
     1921 TGTGGAAGGG ACGGCCGCCT GAGGAGGAAC CCTTCATTAC CCACGGCTTC ACCATGACCA 
     1981 CAGAGGTGCC TCTGCGCGAC GTGCTGGAGG CCATTGCCGA GACTGCCTTC AAGACCTCGC 
     2041 CCTACCCCGT CATCCTCTCC TTCGAGAACC ATGTGGACTC GGCAAAGCAA CAGGCAAAGA 
     2101 TGGCTGAGTA CTGCCGCTCC ATCTTTGGAG ACGCGCTACT CATCGAGCCT CTGGACAAGT 
     2161 ACCCGCTGGC CCCAGGCGTT CCCCTGCCCA GCCCCCAGGA CCTGATGGGC CGTATCCTGG 
     2221 TGAAGAACAA GAAGCGGCAC CGACCCAGCG CAGGTGGCCC AGACAGCGCC GGGCGCAAGC 
     2281 GGCCCCTGGA GCAGAGCAAT TCTGCCCTGA GCGAGAGCTC CGCGGCCACC GAGCCCTCCT 
     2341 CCCCGCAGCT GGGGTCTCCC AGCTCTGACA GCTGCCCAGG CCTGAGCAAT GGGGAGGAGG 
     2401 TAGGGCTTGA GAAGCCCAGC CTGGAGCCTC AGAAGTCTCT GGGTGACGAG GGCCTGAACC 
     2461 GAGGCCCCTA TGTTCTTGGA CCTGCTGACC GTGAGGATGA GGAGGAAGAT GAGGAAGAGG 
     2521 AGGAACAGAC AGACCCCAAA AAGCCAACTA CAGATGAGGG CACAGCCAGC AGCGAGGTGA 
     2581 ATGCCACTGA GGAGATGTCC ACGCTTGTCA ACTACATCGA ACCTGTCAAG TTCAAGTCCT 
     2641 TTGAGGCTGC TCGAAAGAGG AACAAATGCT TCGAGATGTC GTCCTTTGTG GAGACCAAGG 
     2701 CCATGGAGCA ACTGACCAAG AGCCCCATGG AGTTTGTGGA ATACAACAAG CAGCAGCTCA 
     2761 GCCGCATCTA CCCCAAGGGC ACCCGCGTGG ACTCCTCCAA CTACATGCCC CAGCTCTTCT 
     2821 GGAACGTAGG GTGCCAGCTT GTTGCGCTCA ACTTCCAGAC CCTCGATGTG GCGATGCAGC 
     2881 TCAACGCGGG CGTTTTTGAG TACAACGGGC GCAGCGGGTA CCTGCTCAAG CCGGAGTTCA 
     2941 TGCGGCGGCC GGACAAGTCC TTCGACCCCT TCACTGAGGT CATCGTGGAT GGCATCGTGG 
     3001 CCAATGCCTT GCGGGTCAAG GTGATCTCAG GGCAGTTCCT GTCCGACAGG AAGGTGGGCA 
     3061 TCTACGTGGA GGTGGACATG TTTGGCCTCC CTGTTGATAC GCGGCGCAAG TACCGCACCC 
     3121 GGACCTCTCA GGGGAACTCG TTCAACCCCG TGTGGGACGA AGAGCCCTTC GACTTCCCCA 
     3181 AGGTGGTGCT GCCCACGCTG GCTTCACTTC GCATTGCAGC CTTTGAGGAG GGGGGTAAAT 
     3241 TCGTAGGGCA CCGGATCCTG CCTGTCTCTG CCATCCGCTC CGGATACCAC TACGTCTGCC 
     3301 TGCGGAACGA GGCCAACCAA CCGCTGTGCC TGCCGGCCCT GCTCATCTAC ACCGAAGCCT 
     3361 CGGACTACAT TCCTGACGAC CACCAGGACT ATGCGGAGGC CCTGATCAAC CCCATTAAGC 
     3421 ACGTCAGCCT GATGGACCAG AGGGCCCGGC AGCTGGCCGC CCTCATTGGG GAGAGTGAGG 
     3481 CTCAGGCTGG CCAAGAGACG TGCCAGGACA CCCAGTCTCA GCAGCTGGGG TCTCAGCCGT 
     3541 CCTCAAACCC CACCCCCAGC CCACTGGATG CCTCCCCCCG CCGGCCCCCT GGCCCCACCA 
     3601 CCTCCCCTGC CAGCACCTCC CTCAGCAGCC CAGGGCAGCG TGATGATCTC ATCGCCAGCA 
     3661 TCCTCTCAGA GGTGGCCCCC ACCCCGCTGG ATGAGCTCCG AGGTCACAAG GCTCTGGTCA 
     3721 AGCTCCGGAG CCGGCAAGAG CGAGACCTGC GGGAGCTGCG CAAGAAGCAT CAGCGGAAGG 
     3781 CAGTCACCCT CACCCGCCGC CTGCTGGATG GCCTGGCTCA GGCACAGGCT GAGGGCAGGT 
     3841 GCCGGCTGCG GCCAGGTGCC CTAGGTGGGG CCGCTGATGT GGAGGACACG AAGGAGGGGG 
     3901 AGGACGAGGC AAAGCGGTAT CAGGAGTTCC AGAACAGACA GGTGCAGAGC CTGCTGGAGC 
     3961 TGCGGGAGGC CCAGGTGGAC GCAGAGGCCC AGCGGAGGCT GGAACACCTG AGACAGGCTC 
     4021 TGCAGCGGCT CAGGGAGGTC GTCCTTGATG CAAACACAAC TCAGTTCAAG AGGCTGAAAG 
     4081 AGATGAACGA GAGGGAGAAG AAGGAGCTGC AGAAGATCCT GGACAGAAAG CGCCATAACA 
     4141 GCATCTCGGA GGCCAAGATG AGGGACAAGC ATAAGAAGGA GGCGGAACTG ACGGAGATTA 
     4201 ACCGTCGGCA CATCACTGAG TCAGTCAACT CCATCCGTCG GCTGGAGGAG GCCCAGAAGC 
     4261 AGCGGCATGA CCGTCTTGTG GCTGGGCAGC AGCAGGTCCT GCAACAGCTG GCAGAAGAGG 
     4321 AGCCCAAGCT GCTGGCCCAG CTGGCCCAGG AGTGTCAGGA GCAGCGGGCG AGGCTCCCCC 
     4381 AGGAGATCCG CCGGAGCCTG CTGGGCGAGA TGCCGGAGGG GCTGGGGGAC GGGCCTCTGG 
     4441 TGGCCTGTGC CAGCAACGGT CACGCACCCG GGAGCAGCGG GCACCTGTCG GGCGCTGACT 
     4501 CGGAGAGCCA GGAGGAGAAC ACGCAGCTCA CGCGTACGCG GCCGCTCGAG CAGAAACTCA 
     4561 TCTCAGAAGA GGATCTGGCA GCAAATGATA TCCTGGATTA CAAGGATGAC GACGATAAGG 
     4621 TTTAAACGGC CGGCCGCGGT CATAGCTGTT TCCTGAACAG ATCCCGGGTG GCATCCCTGT 
     4681 GACCCCTCCC CAGTGCCTCT CCTGGCCCTG GAAGTTGCCA CTCCAGTGCC CACCAGCCTT 
     4741 GTCCTAATAA AATTAAGTTG CATCATTTTG TCTGACTAGG TGTCCTTCTA TAATATTATG 
     4801 GGGTGGAGGG GGGTGGTATG GAGCAAGGGG CAAGTTGGGA AGACAACCTG TAGGGCCTGC 
     4861 GGGGTCTATT GGGAACCAAG CTGGAGTGCA GTGGCACAAT CTTGGCTCAC TGCAATCTCC 
     4921 GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA 
     4981 TGCATGACCA GGCTCAGCTA ATTTTTGTTT TTTTGGTAGA GACGGGGTTT CACCATATTG 
     5041 GCCAGGCTGG TCTCCAACTC CTAATCTCAG GTGATCTACC CACCTTGGCC TCCCAAATTG 
     5101 CTGGGATTAC AGGCGTGAAC CACTGCTCCC TTCCCTGTCC TTCTGATTTT AAAATAACTA 
     5161 TACCAGCAGG AGGACGTCCA GACACAGCAT AGGCTACCTG GCCATGCCCA ACCGGTGGGA 
     5221 CATTTGAGTT GCTTGCTTGG CACTGTCCTC TCATGCGTTG GGTCCACTCA GTAGATGCCT 
     5281 GTTGAATTGG GTACGCGGCC AGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG 
     5341 GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA 
     5401 GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA 
     5461 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG 
     5521 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT 
     5581 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG 
     5641 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC 
     5701 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT 
     5761 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA 
     5821 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC 
     5881 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC 
     5941 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT 
     6001 TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC 
     6061 TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT 
     6121 CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA 
     6181 ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC GACGTTGGCT GCGAGCCCTG GGCCTTCACC 
     6241 CGAACTTGGG GGGTGGGGTG GGGAAAAGGA AGAAACGCGG GCGTATTGGC CCCAATGGGG 
     6301 TCTCGGTGGG GTATCGACAG AGTGCCAGCC CTGGGACCGA ACCCCGCGTT TATGAACAAA 
     6361 CGACCCAACA CCGTGCGTTT TATTCTGTCT TTTTATTGCC GTCATAGCGC GGGTTCCTTC 
     6421 CGGTATTGTC TCCTTCCGTG TTTCAGTTAG CCTCCCCCTA GGGTGGGCGA AGAACTCCAG 
     6481 CATGAGATCC CCGCGCTGGA GGATCATCCA GCCGGCGTCC CGGAAAACGA TTCCGAAGCC 
     6541 CAACCTTTCA TAGAAGGCGG CGGTGGAATC GAAATCTCGT GATGGCAGGT TGGGCGTCGC 
     6601 TTGGTCGGTC ATTTCGAACC CCAGAGTCCC GCTCAGAAGA ACTCGTCAAG AAGGCGATAG 
     6661 AAGGCGATGC GCTGCGAATC GGGAGCGGCG ATACCGTAAA GCACGAGGAA GCGGTCAGCC 
     6721 CATTCGCCGC CAAGCTCTTC AGCAATATCA CGGGTAGCCA ACGCTATGTC CTGATAGCGA 
     6781 TCCGCCACAC CCAGCCGGCC ACAGTCGATG AATCCAGAAA AGCGGCCATT TTCCACCATG 
     6841 ATATTCGGCA AGCAGGCATC GCCATGGGTC ACGACGAGAT CCTCGCCGTC GGGCATGCTC 
     6901 GCCTTGAGCC TGGCGAACAG TTCGGCTGGC GCGAGCCCCT GATGCTCTTC GTCCAGATCA 
     6961 TCCTGATCGA CAAGACCGGC TTCCATCCGA GTACGTGCTC GCTCGATGCG ATGTTTCGCT 
     7021 TGGTGGTCGA ATGGGCAGGT AGCCGGATCA AGCGTATGCA GCCGCCGCAT TGCATCAGCC 
     7081 ATGATGGATA CTTTCTCGGC AGGAGCAAGG TGAGATGACA GGAGATCCTG CCCCGGCACT 
     7141 TCGCCCAATA GCAGCCAGTC CCTTCCCGCT TCAGTGACAA CGTCGAGCAC AGCTGCGCAA 
     7201 GGAACGCCCG TCGTGGCCAG CCACGATAGC CGCGCTGCCT CGTCTTGCAG TTCATTCAGG 
     7261 GCACCGGACA GGTCGGTCTT GACAAAAAGA ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC 
     7321 ACGGCGGCAT CAGAGCAGCC GATTGTCTGT TGTGCCCAGT CATAGCCGAA TAGCCTCTCC 
     7381 ACCCAAGCGG CCGGAGAACC TGCGTGCAAT CCATCTTGTT CAATCATGCG AAACGATCCT 
     7441 CATCCTGTCT CTTGATCGAT CTTTGCAAAA GCCTAGGCCT CCAAAAAAGC CTCCTCACTA 
     7501 CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC CTCGGCCTCT GCATAAATAA AAAAAATTAG 
     7561 TCAGCCATGG GGCGGAGAAT GGGCGGAACT GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT 
     7621 TAGGGGCGGG ACTATGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     7681 TGGGGAGCCT GGGGACTTTC CACACCTGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     7741 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCCT AACTGACACA CATTCCACAG 
     7801 CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT 
     7861 TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT 
     7921 CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGCGCCCT GTAGCGGCGC ATTAAGCGCG 
     7981 GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT 
     8041 CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA 
     8101 AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA 
     8161 CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT 
     8221 TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC 
     8281 AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG 
     8341 TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TT
//