LOCUS       RC230662                8331 bp    DNA     circular  SYN 
DEFINITION  CACNA2D2 (Myc-DDK-tagged)-Human calcium channel, voltage-dependent, alpha 2/delta subunit 2 (CACNA2D2), transcript variant 3
ACCESSION   NM_001174051
VERSION     NM_001174051.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4488..4579 
                     /label="Myc-DDK"  
     misc_feature    4609..5194 
                     /label="PolyA signal"  
     misc_feature    4666..4685 
                     /label="Restriction Enzyme"  
     misc_feature    5470..6142 
                     /label="ColE1"  
     misc_feature    6572..7366 
                     /label="Kan/Neo"  
     misc_feature    7401..7703 
                     /label="SV40 ori"  
     misc_feature    7789..8266 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4470..4476 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4470 
                     /label="CACNA2D2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGGTGCCG GCTCGGACCT GCGGCGCCTC TCGGCCCGGC CCAGCGCGGA 
     1081 CTGCGCGCCC CTGGCCCGGC TGCGGCCCCC ACCCTGGCCC CGGCACCCGG CGCCCGACGT 
     1141 CCGGGCCCCC GCGCCCGCTG TGGCTGCTGC TGCCGCTTCT ACCGCTGCTC GCCGCCCCCG 
     1201 GCGCCTCTGC CTACAGCTTC CCCCAGCAGC ACACGATGCA GCACTGGGCC CGGCGTCTGG 
     1261 AGCAGGAGGT CGACGGCGTG ATGCGGATTT TTGGAGGCGT CCAGCAGCTC CGTGAGATTT 
     1321 ACAAGGACAA CCGGAACCTG TTCGAGGTAC AGGAGAATGA GCCTCAGAAG TTGGTGGAGA 
     1381 AGGTGGCAGG GGACATTGAG AGCCTTCTGG ACAGGAAGGT GCAGGCCCTG AAGAGACTGG 
     1441 CTGATGCTGC AGAGAACTTC CAGAAAGCAC ACCGCTGGCA GGACAACATC AAGGAGGAAG 
     1501 ACATCGTGTA CTATGACGCC AAGGCTGACG CTGAGCTGGA CGACCCTGAG AGTGAGGATG 
     1561 TGGAAAGGGG GTCTAAGGCC AGCACCCTAA GGCTGGACTT CATCGAGGAC CCAAACTTCA 
     1621 AGAACAAGGT CAACTATTCA TACGCGGCTG TACAGATCCC TACGGACATC TACAAAGGCT 
     1681 CCACTGTCAT CCTCAATGAG CTCAACTGGA CAGAGGCCCT GGAGAATGTG TTCATGGAAA 
     1741 ACCGCAGACA AGACCCCACA CTGCTGTGGC AGGTCTTCGG CAGCGCCACA GGAGTCACTC 
     1801 GCTACTACCC GGCCACCCCG TGGCGAGCCC CCAAGAAGAT CGACCTGTAC GATGTCCGAA 
     1861 GGAGACCCTG GTATATCCAG GGGGCCTCGT CACCCAAAGA CATGGTCATC ATCGTGGATG 
     1921 TGAGTGGCAG TGTGAGCGGC CTGACCCTGA AGCTGATGAA GACATCTGTC TGCGAGATGC 
     1981 TGGACACGCT GTCTGATGAT GACTATGTGA ATGTGGCCTC GTTCAACGAG AAGGCACAGC 
     2041 CTGTGTCATG CTTCACACAC CTGGTGCAGG CCAATGTGCG CAACAAGAAG GTGTTCAAGG 
     2101 AAGCTGTGCA GGGCATGGTG GCCAAGGGCA CCACAGGCTA CAAGGCCGGC TTTGAGTATG 
     2161 CCTTTGACCA GCTGCAGAAC TCCAACATCA CTCGGGCCAA CTGCAACAAG ATGATCATGA 
     2221 TGTTCACGGA TGGTGGTGAG GACCGCGTGC AGGACGTCTT TGAGAAGTAC AATTGGCCAA 
     2281 ACCGGACGGT GCGCGTGTTT ACTTTCTCCG TGGGGCAGCA TAACTATGAC GTCACACCGC 
     2341 TGCAGTGGAT GGCCTGTGCC AACAAAGGCT ACTATTTTGA GATCCCTTCC ATCGGAGCCA 
     2401 TCCGCATCAA CACACAGGAA TATCTAGATG TGTTGGGCAG GCCCATGGTG CTGGCAGGCA 
     2461 AGGAGGCCAA GCAGGTGCAG TGGACCAACG TGTATGAGGA TGCACTGGGA CTGGGGTTGG 
     2521 TGGTAACAGG GACCCTCCCT GTTTTCAACC TGACACAGGA TGGCCCTGGG GAAAAGAAGA 
     2581 ACCAGCTGAT CCTGGGCGTG ATGGGCATTG ACGTGGCTCT GAATGACATC AAGAGGCTGA 
     2641 CCCCCAACTA CACGCTTGGA GCCAACGGCT ATGTGTTTGC CATTGACCTG AACGGCTACG 
     2701 TGTTGCTGCA CCCCAATCTC AAGCCCCAGA CCACCAACTT CCGGGAGCCT GTGACTCTGG 
     2761 ACTTCCTGGA TGCGGAGCTA GAGGATGAGA ACAAGGAAGA GATCCGTCGG AGCATGATTG 
     2821 ATGGCAACAA GGGCCACAAG CAGATCAGAA CGTTGGTCAA GTCCCTGGAT GAGAGGTACA 
     2881 TAGATGAGGT GACACGGAAC TACACCTGGG TGCCTATAAG GAGCACTAAC TACAGCCTGG 
     2941 GGCTGGTGCT CCCACCCTAC AGCACCTTCT ACCTCCAAGC CAATCTCAGT GACCAGATCC 
     3001 TGCAGGTCAA GTTGCCAATC AGCAAACTGA AGGATTTTGA GTTCCTGCTC CCCAGCAGCT 
     3061 TTGAGTCTGA AGGACACGTT TTCATTGCTC CCAGAGAGTA CTGCAAGGAC CTGAATGCCT 
     3121 CAGACAACAA CACCGAGTTC CTGAAAAACT TTATTGAGCT CATGGAGAAA GTGACTCCAG 
     3181 ACTCCAAGCA GTGCAACAAC TTCCTTCTGC ACAACCTGAT CTTGGACACG GGCATCACGC 
     3241 AGCAGCTGGT AGAGCGTGTG TGGAGGGACC AGGATCTCAA CACGTACAGC CTACTGGCCG 
     3301 TGTTCGCTGC CACAGACGGT GGCATCACCC GAGTCTTCCC CAACAAGGCA GCTGAGGACT 
     3361 GGACAGAGAA CCCTGAGCCC TTCAATGCCA GCTTCTACCG CCGCAGCCTG GATAACCACG 
     3421 GTTATGTCTT CAAGCCCCCA CACCAGGATG CCCTGTTAAG GCCGCTGGAG CTGGAGAATG 
     3481 ACACTGTGGG CATCCTCGTC AGCACAGCTG TGGAGCTCAG CCTAGGCAGG CGCACACTGA 
     3541 GGCCAGCAGT GGTGGGCGTC AAGCTGGACC TAGAGGCTTG GGCTGAGAAG TTCAAGGTGC 
     3601 TAGCCAGCAA CCGTACCCAC CAAGACCAGC CTCAGAAGTG CGGCCCCAAC AGCCACTGTG 
     3661 AGATGGACTG CGAGGTTAAC AATGAGGACT TACTCTGTGT CCTCATTGAT GATGGAGGAT 
     3721 TCCTGGTGCT GTCAAACCAG AACCATCAGT GGGACCAGGT GGGCAGGTTC TTCAGTGAGG 
     3781 TGGATGCCAA CCTGATGCTG GCACTCTACA ATAACTCCTT CTACACCCGC AAGGAGTCCT 
     3841 ATGACTATCA GGCAGCCTGT GCCCCTCAGC CCCCTGGCAA CCTGGGTGCT GCACCCCGGG 
     3901 GTGTCTTTGT GCCCACCGTT GCAGATTTCC TTAACCTGGC CTGGTGGACC TCTGCTGCCG 
     3961 CCTGGTCCCT GTTCCAGCAG CTTCTCTACG GCCTCATCTA CCACAGCTGG TTCCAAGCAG 
     4021 ACCCCGCGGA GGCCGAGGGG AGCCCCGAGA CGCGCGAGAG CAGCTGCGTC ATGAAACAGA 
     4081 CCCAGTACTA CTTCGGCTCG GTAAACGCCT CCTACAACGC CATCATCGAC TGCGGAAACT 
     4141 GCTCCAGGCT GTTCCACGCG CAGAGACTGA CCAACACCAA TCTTCTCTTT GTGGTGGCCG 
     4201 AGAAGCCGCT GTGCAGCCAG TGCGAGGCTG GCCGGCTGCT GCAGAAGGAG ACGCACTCGG 
     4261 ACGGCCCGGA GCAGTGTGAG CTAGTGCAGA GACCGCGATA CCGGAGAGGC CCGCACATCT 
     4321 GCTTCGACTA CAACGCGACA GAAGATACCT CAGACTGTGG CCGCGGGGCC TCCTTCCCGC 
     4381 CGTCGCTGGG CGTCCTGGTC TCCCTGCAAC TGCTGCTCCT CCTGGGCCTG CCGCCCCGGC 
     4441 CGCAGCCTCA AGTCCTCGTC CACGCCTCTC GCCGCCTCAC GCGTACGCGG CCGCTCGAGC 
     4501 AGAAACTCAT CTCAGAAGAG GATCTGGCAG CAAATGATAT CCTGGATTAC AAGGATGACG 
     4561 ACGATAAGGT TTAAACGGCC GGCCGCGGTC ATAGCTGTTT CCTGAACAGA TCCCGGGTGG 
     4621 CATCCCTGTG ACCCCTCCCC AGTGCCTCTC CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC 
     4681 ACCAGCCTTG TCCTAATAAA ATTAAGTTGC ATCATTTTGT CTGACTAGGT GTCCTTCTAT 
     4741 AATATTATGG GGTGGAGGGG GGTGGTATGG AGCAAGGGGC AAGTTGGGAA GACAACCTGT 
     4801 AGGGCCTGCG GGGTCTATTG GGAACCAAGC TGGAGTGCAG TGGCACAATC TTGGCTCACT 
     4861 GCAATCTCCG CCTCCTGGGT TCAAGCGATT CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA 
     4921 TTCCAGGCAT GCATGACCAG GCTCAGCTAA TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC 
     4981 ACCATATTGG CCAGGCTGGT CTCCAACTCC TAATCTCAGG TGATCTACCC ACCTTGGCCT 
     5041 CCCAAATTGC TGGGATTACA GGCGTGAACC ACTGCTCCCT TCCCTGTCCT TCTGATTTTA 
     5101 AAATAACTAT ACCAGCAGGA GGACGTCCAG ACACAGCATA GGCTACCTGG CCATGCCCAA 
     5161 CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC ACTGTCCTCT CATGCGTTGG GTCCACTCAG 
     5221 TAGATGCCTG TTGAATTGGG TACGCGGCCA GCGGCGAGCG GTATCAGCTC ACTCAAAGGC 
     5281 GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG 
     5341 CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG 
     5401 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG 
     5461 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC 
     5521 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA 
     5581 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT 
     5641 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC 
     5701 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG 
     5761 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC 
     5821 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT 
     5881 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA 
     5941 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG 
     6001 GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA 
     6061 AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT 
     6121 ATATGAGTAA CCTGAGGCTA TGGCAGGGCC TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG 
     6181 GCCTTCACCC GAACTTGGGG GGTGGGGTGG GGAAAAGGAA GAAACGCGGG CGTATTGGCC 
     6241 CCAATGGGGT CTCGGTGGGG TATCGACAGA GTGCCAGCCC TGGGACCGAA CCCCGCGTTT 
     6301 ATGAACAAAC GACCCAACAC CGTGCGTTTT ATTCTGTCTT TTTATTGCCG TCATAGCGCG 
     6361 GGTTCCTTCC GGTATTGTCT CCTTCCGTGT TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA 
     6421 GAACTCCAGC ATGAGATCCC CGCGCTGGAG GATCATCCAG CCGGCGTCCC GGAAAACGAT 
     6481 TCCGAAGCCC AACCTTTCAT AGAAGGCGGC GGTGGAATCG AAATCTCGTG ATGGCAGGTT 
     6541 GGGCGTCGCT TGGTCGGTCA TTTCGAACCC CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA 
     6601 AGGCGATAGA AGGCGATGCG CTGCGAATCG GGAGCGGCGA TACCGTAAAG CACGAGGAAG 
     6661 CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA GCAATATCAC GGGTAGCCAA CGCTATGTCC 
     6721 TGATAGCGAT CCGCCACACC CAGCCGGCCA CAGTCGATGA ATCCAGAAAA GCGGCCATTT 
     6781 TCCACCATGA TATTCGGCAA GCAGGCATCG CCATGGGTCA CGACGAGATC CTCGCCGTCG 
     6841 GGCATGCTCG CCTTGAGCCT GGCGAACAGT TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG 
     6901 TCCAGATCAT CCTGATCGAC AAGACCGGCT TCCATCCGAG TACGTGCTCG CTCGATGCGA 
     6961 TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA GCCGGATCAA GCGTATGCAG CCGCCGCATT 
     7021 GCATCAGCCA TGATGGATAC TTTCTCGGCA GGAGCAAGGT GAGATGACAG GAGATCCTGC 
     7081 CCCGGCACTT CGCCCAATAG CAGCCAGTCC CTTCCCGCTT CAGTGACAAC GTCGAGCACA 
     7141 GCTGCGCAAG GAACGCCCGT CGTGGCCAGC CACGATAGCC GCGCTGCCTC GTCTTGCAGT 
     7201 TCATTCAGGG CACCGGACAG GTCGGTCTTG ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC 
     7261 AGCCGGAACA CGGCGGCATC AGAGCAGCCG ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT 
     7321 AGCCTCTCCA CCCAAGCGGC CGGAGAACCT GCGTGCAATC CATCTTGTTC AATCATGCGA 
     7381 AACGATCCTC ATCCTGTCTC TTGATCGATC TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC 
     7441 TCCTCACTAC TTCTGGAATA GCTCAGAGGC CGAGGCGGCC TCGGCCTCTG CATAAATAAA 
     7501 AAAAATTAGT CAGCCATGGG GCGGAGAATG GGCGGAACTG GGCGGAGTTA GGGGCGGGAT 
     7561 GGGCGGAGTT AGGGGCGGGA CTATGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     7621 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCTGGTT GCTGACTAAT TGAGATGCAT 
     7681 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCCTA ACTGACACAC 
     7741 ATTCCACAGC TGGTTCTTTC CGCCTCAGGA CTCTTCCTTT TTCAATATTA TTGAAGCATT 
     7801 TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA 
     7861 ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGCGCCCTG TAGCGGCGCA 
     7921 TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA 
     7981 GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT 
     8041 CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC 
     8101 CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT 
     8161 TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA 
     8221 ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG 
     8281 GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT T
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