LOCUS       RC230646                8145 bp    DNA     circular  SYN 
DEFINITION  CASR (Myc-DDK-tagged)-Human calcium-sensing receptor (CASR), transcript variant 1
ACCESSION   NM_001178065
VERSION     NM_001178065.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4302..4393 
                     /label="Myc-DDK"  
     misc_feature    4423..5008 
                     /label="PolyA signal"  
     misc_feature    4480..4499 
                     /label="Restriction Enzyme"  
     misc_feature    5284..5956 
                     /label="ColE1"  
     misc_feature    6386..7180 
                     /label="Kan/Neo"  
     misc_feature    7215..7517 
                     /label="SV40 ori"  
     misc_feature    7603..8080 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4284..4290 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4284 
                     /label="CASR"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCATTTTAT AGCTGCTGCT GGGTCCTCTT GGCACTCACC TGGCACACCT 
     1081 CTGCCTACGG GCCAGACCAG CGAGCCCAAA AGAAGGGGGA CATTATCCTT GGGGGGCTCT 
     1141 TTCCTATTCA TTTTGGAGTA GCAGCTAAAG ATCAAGATCT CAAATCAAGG CCGGAGTCTG 
     1201 TGGAATGTAT CAGGTATAAT TTCCGTGGGT TTCGCTGGTT ACAGGCTATG ATATTTGCCA 
     1261 TAGAGGAGAT AAACAGCAGC CCAGCCCTTC TTCCCAACTT GACGCTGGGA TACAGGATAT 
     1321 TTGACACTTG CAACACCGTT TCTAAGGCCT TGGAAGCCAC CCTGAGTTTT GTTGCTCAAA 
     1381 ACAAAATTGA TTCTTTGAAC CTTGATGAGT TCTGCAACTG CTCAGAGCAC ATTCCCTCTA 
     1441 CGATTGCTGT GGTGGGAGCA ACTGGCTCAG GCGTCTCCAC GGCAGTGGCA AATCTGCTGG 
     1501 GGCTCTTCTA CATTCCCCAG GTCAGTTATG CCTCCTCCAG CAGACTCCTC AGCAACAAGA 
     1561 ATCAATTCAA GTCTTTCCTC CGAACCATCC CCAATGATGA GCACCAGGCC ACTGCCATGG 
     1621 CAGACATCAT CGAGTATTTC CGCTGGAACT GGGTGGGCAC AATTGCAGCT GATGACGACT 
     1681 ATGGGCGGCC GGGGATTGAG AAATTCCGAG AGGAAGCTGA GGAAAGGGAT ATCTGCATCG 
     1741 ACTTCAGTGA ACTCATCTCC CAGTACTCTG ATGAGGAAGA GATCCAGCAT GTGGTAGAGG 
     1801 TGATTCAAAA TTCCACGGCC AAAGTCATCG TGGTTTTCTC CAGTGGCCCA GATCTTGAGC 
     1861 CCCTCATCAA GGAGATTGTC CGGCGCAATA TCACGGGCAA GATCTGGCTG GCCAGCGAGG 
     1921 CCTGGGCCAG CTCCTCCCTG ATCGCCATGC CTCAGTACTT CCACGTGGTT GGCGGCACCA 
     1981 TTGGATTCGC TCTGAAGGCT GGGCAGATCC CAGGCTTCCG GGAATTCCTG AAGAAGGTCC 
     2041 ATCCCAGGAA GTCTGTCCAC AATGGTTTTG CCAAGGAGTT TTGGGAAGAA ACATTTAACT 
     2101 GCCACCTCCA AGAAGGTGCA AAAGGACCTT TACCTGTGGA CACCTTTCTG AGAGGTCACG 
     2161 AAGAAAGTGG CGACAGGTTT AGCAACAGCT CGACAGCCTT CCGACCCCTC TGTACAGGGG 
     2221 ATGAGAACAT CAGCAGTGTC GAGACCCCTT ACATAGATTA CACGCATTTA CGGATATCCT 
     2281 ACAATGTGTA CTTAGCAGTC TACTCCATTG CCCACGCCTT GCAAGATATA TATACCTGCT 
     2341 TACCTGGGAG AGGGCTCTTC ACCAATGGCT CCTGTGCAGA CATCAAGAAA GTTGAGGCGT 
     2401 GGCAGGTCCT GAAGCACCTA CGGCATCTAA ACTTTACAAA CAATATGGGG GAGCAGGTGA 
     2461 CCTTTGATGA GTGTGGTGAC CTGGTGGGGA ACTATTCCAT CATCAACTGG CACCTCTCCC 
     2521 CAGAGGATGG CTCCATCGTG TTTAAGGAAG TCGGGTATTA CAACGTCTAT GCCAAGAAGG 
     2581 GAGAAAGACT CTTCATCAAC GAGGAGAAAA TCCTGTGGAG TGGGTTCTCC AGGGAGCCAC 
     2641 TCACCTTTGT GCTGTCTGTC CTCCAGGTGC CCTTCTCCAA CTGCAGCCGA GACTGCCTGG 
     2701 CAGGGACCAG GAAAGGGATC ATTGAGGGGG AGCCCACCTG CTGCTTTGAG TGTGTGGAGT 
     2761 GTCCTGATGG GGAGTATAGT GATGAGACAG ATGCCAGTGC CTGTAACAAG TGCCCAGATG 
     2821 ACTTCTGGTC CAATGAGAAC CACACCTCCT GCATTGCCAA GGAGATCGAG TTTCTGTCGT 
     2881 GGACGGAGCC CTTTGGGATC GCACTCACCC TCTTTGCCGT GCTGGGCATT TTCCTGACAG 
     2941 CCTTTGTGCT GGGTGTGTTT ATCAAGTTCC GCAACACACC CATTGTCAAG GCCACCAACC 
     3001 GAGAGCTCTC CTACCTCCTC CTCTTCTCCC TGCTCTGCTG CTTCTCCAGC TCCCTGTTCT 
     3061 TCATCGGGGA GCCCCAGGAC TGGACGTGCC GCCTGCGCCA GCCGGCCTTT GGCATCAGCT 
     3121 TCGTGCTCTG CATCTCATGC ATCCTGGTGA AAACCAACCG TGTCCTCCTG GTGTTTGAGG 
     3181 CCAAGATCCC CACCAGCTTC CACCGCAAGT GGTGGGGGCT CAACCTGCAG TTCCTGCTGG 
     3241 TTTTCCTCTG CACCTTCATG CAGATTGTCA TCTGTGTGAT CTGGCTCTAC ACCGCGCCCC 
     3301 CCTCAAGCTA CCGCAACCAG GAGCTGGAGG ATGAGATCAT CTTCATCACG TGCCACGAGG 
     3361 GCTCCCTCAT GGCCCTGGGC TTCCTGATCG GCTACACCTG CCTGCTGGCT GCCATCTGCT 
     3421 TCTTCTTTGC CTTCAAGTCC CGGAAGCTGC CGGAGAACTT CAATGAAGCC AAGTTCATCA 
     3481 CCTTCAGCAT GCTCATCTTC TTCATCGTCT GGATCTCCTT CATTCCAGCC TATGCCAGCA 
     3541 CCTATGGCAA GTTTGTCTCT GCCGTAGAGG TGATTGCCAT CCTGGCAGCC AGCTTTGGCT 
     3601 TGCTGGCGTG CATCTTCTTC AACAAGATCT ACATCATTCT CTTCAAGCCA TCCCGCAACA 
     3661 CCATCGAGGA GGTGCGTTGC AGCACCGCAG CTCACGCTTT CAAGGTGGCT GCCCGGGCCA 
     3721 CGCTGCGCCG CAGCAACGTC TCCCGCAAGC GGTCCAGCAG CCTTGGAGGC TCCACGGGAT 
     3781 CCACCCCCTC CTCCTCCATC AGCAGCAAGA GCAACAGCGA AGACCCATTC CCACAGCCCG 
     3841 AGAGGCAGAA GCAGCAGCAG CCGCTGGCCC TAACCCAGCA AGAGCAGCAG CAGCAGCCCC 
     3901 TGACCCTCCC ACAGCAGCAA CGATCTCAGC AGCAGCCCAG ATGCAAGCAG AAGGTCATCT 
     3961 TTGGCAGCGG CACGGTCACC TTCTCACTGA GCTTTGATGA GCCTCAGAAG AACGCCATGG 
     4021 CCCACAGGAA TTCTACGCAC CAGAACTCCC TGGAGGCCCA GAAAAGCAGC GATACGCTGA 
     4081 CCCGACACCA GCCATTACTC CCGCTGCAGT GCGGGGAAAC GGACTTAGAT CTGACCGTCC 
     4141 AGGAAACAGG TCTGCAAGGA CCTGTGGGTG GAGACCAGCG GCCAGAGGTG GAGGACCCTG 
     4201 AAGAGTTGTC CCCAGCACTT GTAGTGTCCA GTTCACAGAG CTTTGTCATC AGTGGTGGAG 
     4261 GCAGCACTGT TACAGAAAAC GTAGTGAATT CAACGCGTAC GCGGCCGCTC GAGCAGAAAC 
     4321 TCATCTCAGA AGAGGATCTG GCAGCAAATG ATATCCTGGA TTACAAGGAT GACGACGATA 
     4381 AGGTTTAAAC GGCCGGCCGC GGTCATAGCT GTTTCCTGAA CAGATCCCGG GTGGCATCCC 
     4441 TGTGACCCCT CCCCAGTGCC TCTCCTGGCC CTGGAAGTTG CCACTCCAGT GCCCACCAGC 
     4501 CTTGTCCTAA TAAAATTAAG TTGCATCATT TTGTCTGACT AGGTGTCCTT CTATAATATT 
     4561 ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG GGGCAAGTTG GGAAGACAAC CTGTAGGGCC 
     4621 TGCGGGGTCT ATTGGGAACC AAGCTGGAGT GCAGTGGCAC AATCTTGGCT CACTGCAATC 
     4681 TCCGCCTCCT GGGTTCAAGC GATTCTCCTG CCTCAGCCTC CCGAGTTGTT GGGATTCCAG 
     4741 GCATGCATGA CCAGGCTCAG CTAATTTTTG TTTTTTTGGT AGAGACGGGG TTTCACCATA 
     4801 TTGGCCAGGC TGGTCTCCAA CTCCTAATCT CAGGTGATCT ACCCACCTTG GCCTCCCAAA 
     4861 TTGCTGGGAT TACAGGCGTG AACCACTGCT CCCTTCCCTG TCCTTCTGAT TTTAAAATAA 
     4921 CTATACCAGC AGGAGGACGT CCAGACACAG CATAGGCTAC CTGGCCATGC CCAACCGGTG 
     4981 GGACATTTGA GTTGCTTGCT TGGCACTGTC CTCTCATGCG TTGGGTCCAC TCAGTAGATG 
     5041 CCTGTTGAAT TGGGTACGCG GCCAGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT 
     5101 ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA 
     5161 AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC 
     5221 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA 
     5281 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC 
     5341 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC 
     5401 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA 
     5461 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC 
     5521 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG 
     5581 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG 
     5641 AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG 
     5701 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA 
     5761 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA 
     5821 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT 
     5881 CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA 
     5941 GTAACCTGAG GCTATGGCAG GGCCTGCCGC CCCGACGTTG GCTGCGAGCC CTGGGCCTTC 
     6001 ACCCGAACTT GGGGGGTGGG GTGGGGAAAA GGAAGAAACG CGGGCGTATT GGCCCCAATG 
     6061 GGGTCTCGGT GGGGTATCGA CAGAGTGCCA GCCCTGGGAC CGAACCCCGC GTTTATGAAC 
     6121 AAACGACCCA ACACCGTGCG TTTTATTCTG TCTTTTTATT GCCGTCATAG CGCGGGTTCC 
     6181 TTCCGGTATT GTCTCCTTCC GTGTTTCAGT TAGCCTCCCC CTAGGGTGGG CGAAGAACTC 
     6241 CAGCATGAGA TCCCCGCGCT GGAGGATCAT CCAGCCGGCG TCCCGGAAAA CGATTCCGAA 
     6301 GCCCAACCTT TCATAGAAGG CGGCGGTGGA ATCGAAATCT CGTGATGGCA GGTTGGGCGT 
     6361 CGCTTGGTCG GTCATTTCGA ACCCCAGAGT CCCGCTCAGA AGAACTCGTC AAGAAGGCGA 
     6421 TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG GCGATACCGT AAAGCACGAG GAAGCGGTCA 
     6481 GCCCATTCGC CGCCAAGCTC TTCAGCAATA TCACGGGTAG CCAACGCTAT GTCCTGATAG 
     6541 CGATCCGCCA CACCCAGCCG GCCACAGTCG ATGAATCCAG AAAAGCGGCC ATTTTCCACC 
     6601 ATGATATTCG GCAAGCAGGC ATCGCCATGG GTCACGACGA GATCCTCGCC GTCGGGCATG 
     6661 CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT GGCGCGAGCC CCTGATGCTC TTCGTCCAGA 
     6721 TCATCCTGAT CGACAAGACC GGCTTCCATC CGAGTACGTG CTCGCTCGAT GCGATGTTTC 
     6781 GCTTGGTGGT CGAATGGGCA GGTAGCCGGA TCAAGCGTAT GCAGCCGCCG CATTGCATCA 
     6841 GCCATGATGG ATACTTTCTC GGCAGGAGCA AGGTGAGATG ACAGGAGATC CTGCCCCGGC 
     6901 ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC GCTTCAGTGA CAACGTCGAG CACAGCTGCG 
     6961 CAAGGAACGC CCGTCGTGGC CAGCCACGAT AGCCGCGCTG CCTCGTCTTG CAGTTCATTC 
     7021 AGGGCACCGG ACAGGTCGGT CTTGACAAAA AGAACCGGGC GCCCCTGCGC TGACAGCCGG 
     7081 AACACGGCGG CATCAGAGCA GCCGATTGTC TGTTGTGCCC AGTCATAGCC GAATAGCCTC 
     7141 TCCACCCAAG CGGCCGGAGA ACCTGCGTGC AATCCATCTT GTTCAATCAT GCGAAACGAT 
     7201 CCTCATCCTG TCTCTTGATC GATCTTTGCA AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA 
     7261 CTACTTCTGG AATAGCTCAG AGGCCGAGGC GGCCTCGGCC TCTGCATAAA TAAAAAAAAT 
     7321 TAGTCAGCCA TGGGGCGGAG AATGGGCGGA ACTGGGCGGA GTTAGGGGCG GGATGGGCGG 
     7381 AGTTAGGGGC GGGACTATGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     7441 TGCTGGGGAG CCTGGGGACT TTCCACACCT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     7501 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CCTAACTGAC ACACATTCCA 
     7561 CAGCTGGTTC TTTCCGCCTC AGGACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG 
     7621 GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG 
     7681 GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGCGC CCTGTAGCGG CGCATTAAGC 
     7741 GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC 
     7801 GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT 
     7861 CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA 
     7921 AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC 
     7981 CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA 
     8041 CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT 
     8101 TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTT
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