LOCUS       RC230559                7524 bp    DNA     circular  SYN 
DEFINITION  CLCN2 (Myc-DDK-tagged)-Human chloride channel 2 (CLCN2), transcript variant 2
ACCESSION   NM_001171087
VERSION     NM_001171087.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3681..3772 
                     /label="Myc-DDK"  
     misc_feature    3802..4387 
                     /label="PolyA signal"  
     misc_feature    3859..3878 
                     /label="Restriction Enzyme"  
     misc_feature    4663..5335 
                     /label="ColE1"  
     misc_feature    5765..6559 
                     /label="Kan/Neo"  
     misc_feature    6594..6896 
                     /label="SV40 ori"  
     misc_feature    6982..7459 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3663..3669 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3663 
                     /label="CLCN2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGGCCGCG GCGGCGGAGG AAGGGATGGA GCCACGGGCG CTGCAGTACG 
     1081 AGCAGACCCT GATGTATGGC CGGTACACTC AGGACCTTGG GGCCTTTGCC AAAGAGGAAG 
     1141 CTGCTCGGAT TCGCCTGGGA GGGCCTGAAC CCTGGAAAGG TCCCCCTTCC TCTCGGGCTG 
     1201 CCCCAGAGCT CTTGGAATAT GGACGGAGCC GTTGCGCCCG ATGCCGCGTC TGTTCTGTCC 
     1261 GCTGCCACAA GTTCCTAGTA TCCAGGGTTG GTGAAGATTG GATCTTCCTG GTCCTGCTGG 
     1321 GGCTTCTCAT GGCATTGGTC AGCTGGGTCA TGGACTATGC CATTGCTGCC TGTCTGCAAG 
     1381 CCCAGCAGTG GATGTCCCGG GGCTTGAACA CCAGCATCTT GCTCCAGTAC CTGGCCTGGG 
     1441 TCACCTACCC TGTTGTCCTC ATCACTTTCT CAGCCGGATT CACACAGATC CTGGCCCCTC 
     1501 AGGCTGTCGG CTCTGGCATC CCTGAGATGA AGACCATCTT GCGGGGAGTG GTGCTGAAAG 
     1561 AATACCTCAC ACTCAAGACC TTTATAGCTA AGGTCATTGG GCTGACCTGC GCCCTAGGCA 
     1621 GCGGGATGCC GCTTGGCAAA GAGGGCCCTT TTGTGCATAT CGCAAGCATG TGTGCTGCCC 
     1681 TTCTCAGCAA GTTCCTCTCC CTCTTTGGGG GTATCTATGA GAATGAATCC CGGAACACAG 
     1741 AGATGCTGGC TGCCGCCTGT GCCGTGGGGG TGGGCTGCTG CTTCGCGGCA CCTATTGGAG 
     1801 GCGTCCTCTT CAGCATCGAG GTCACCTCCA CCTTCTTTGC AGTGCGGAAC TACTGGCGGG 
     1861 GCTTCTTCGC TGCCACCTTC AGTGCCTTCA TCTTCCGGGT CTTGGCAGTC TGGAACCGGG 
     1921 ATGAAGAGAC TATTACAGCC CTCTTCAAAA CCCGATTCCG GCTCGACTTC CCCTTTGACC 
     1981 TGCAGGAGCT GCCAGCCTTT GCTGTCATTG GTATTGCTAG TGGCTTCGGT GGAGCCCTCT 
     2041 TTGTCTACCT GAACCGGAAG ATTGTCCAGG TGATGCGGAA GCAGAAAACC ATCAATCGCT 
     2101 TCCTCATGAG GAAACGCCTG CTCTTCCCGG CTCTGGTGAC CCTGCTCATC TCCACGCTGA 
     2161 CCTTCCCCCC TGGCTTTGGA CAGTTCATGG CTGGACAGCT CTCACAGAAA GAGACGCTGG 
     2221 TCACCCTGTT TGACAATCGG ACGTGGGTCC GCCAGGGCCT GGTGGAGGAG CTAGAACCAC 
     2281 CCAGCACCTC ACAGGCCTGG AACCCACCAC GTGCCAACGT CTTCCTCACC CTGGTCATCT 
     2341 TCATTCTCAT GAAGTTCTGG ATGTCTGCAC TGGCCACCAC CATCCCAGTT CCCTGTGGGG 
     2401 CCTTCATGCC TGTCTTTGTC ATTGATGGAA TTCATACGGA CAGCAGCACC TACCGGATTG 
     2461 TGCCTGGGGG CTACGCTGTG GTCGGGGCAG CTGCGCTGGC AGGAGCGGTG ACACACACAG 
     2521 TGTCCACGGC TGTGATCGTG TTCGAGCTCA CAGGCCAGAT TGCCCACATC CTGCCTGTCA 
     2581 TGATCGCCGT CATCCTGGCC AACGCTGTCG CCCAGAGTCT GCAGCCCTCC CTCTATGACA 
     2641 GCATCATCCG AATCAAGAAA CTGCCCTACC TGCCTGAGCT CGGCTGGGGC CGCCACCAGC 
     2701 AGTACCGGGT GCGTGTGGAG GACATCATGG TGCGGGATGT TCCCCATGTG GCCCTCAGCT 
     2761 GCACCTTCCG GGACCTGCGT TTGGCACTGC ACAGGACCAA GGGCCGAATG CTGGCCCTAG 
     2821 TGGAGTCCCC TGAGTCCATG ATTCTGCTGG GCTCCATCGA GCGTTCACAG GTGGTGGCAT 
     2881 TGTTGGGGGC CCAGCTGAGC CCAGCCCGCC GGCGGCAGCA CATGCAGGAG CGCAGAGCCA 
     2941 CCCAGACCTC TCCACTATCT GATCAGGAGG GTCCCCCTAC CCCTGAGGCT TCTGTCTGCT 
     3001 TCCAGGTGAA CACAGAAGAC TCAGCCTTCC CAGCAGCCCG GGGGGAGACC CACAAGCCCC 
     3061 TAAAGCCTGC ACTCAAGAGG GGGCCCAGTG TCACCAGGAA CCTCGGAGAG AGTCCCACAG 
     3121 GGAGCGCAGA GTCGGCAGGC ATCGCCCTCC GGAGCCTCTT CTGTGGCAGT CCACCCCCTG 
     3181 AGGCTGCTTC GGAGAAGTTG GAATCCTGTG AGAAGCGCAA GCTGAAGCGT GTCCGAATCT 
     3241 CCCTGGCAAG TGACGCGGAC CTGGAAGGCG AGATGAGCCC TGAAGAGATT CTGGAGTGGG 
     3301 AGGAGCAGCA ACTAGATGAA CCTGTCAACT TCAGTGACTG CAAAATTGAT CCTGCTCCCT 
     3361 TCCAGCTGGT GGAGCGGACC TCTTTGCACA AGACTCACAC TATCTTCTCA CTGCTGGGAG 
     3421 TGGACCATGC TTATGTCACC AGTATTGGCA GACTCATTGG AATCGTTACT CTAAAGGAGC 
     3481 TCCGGAAGGC CATCGAGGGC TCTGTCACAG CACAGGGTGT GAAAGTCCGG CCGCCCCTCG 
     3541 CCAGCTTCCG AGACAGTGCC ACCAGCAGCA GTGACACGGA GACCACTGAG GTGCATGCAC 
     3601 TCTGGGGGCC CCACTCCCGT CATGGCCTCC CCCGGGAGGG CAGCCCTTCC GACAGCGACG 
     3661 ACAAATGCCA AACGCGTACG CGGCCGCTCG AGCAGAAACT CATCTCAGAA GAGGATCTGG 
     3721 CAGCAAATGA TATCCTGGAT TACAAGGATG ACGACGATAA GGTTTAAACG GCCGGCCGCG 
     3781 GTCATAGCTG TTTCCTGAAC AGATCCCGGG TGGCATCCCT GTGACCCCTC CCCAGTGCCT 
     3841 CTCCTGGCCC TGGAAGTTGC CACTCCAGTG CCCACCAGCC TTGTCCTAAT AAAATTAAGT 
     3901 TGCATCATTT TGTCTGACTA GGTGTCCTTC TATAATATTA TGGGGTGGAG GGGGGTGGTA 
     3961 TGGAGCAAGG GGCAAGTTGG GAAGACAACC TGTAGGGCCT GCGGGGTCTA TTGGGAACCA 
     4021 AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT CCGCCTCCTG GGTTCAAGCG 
     4081 ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG CATGCATGAC CAGGCTCAGC 
     4141 TAATTTTTGT TTTTTTGGTA GAGACGGGGT TTCACCATAT TGGCCAGGCT GGTCTCCAAC 
     4201 TCCTAATCTC AGGTGATCTA CCCACCTTGG CCTCCCAAAT TGCTGGGATT ACAGGCGTGA 
     4261 ACCACTGCTC CCTTCCCTGT CCTTCTGATT TTAAAATAAC TATACCAGCA GGAGGACGTC 
     4321 CAGACACAGC ATAGGCTACC TGGCCATGCC CAACCGGTGG GACATTTGAG TTGCTTGCTT 
     4381 GGCACTGTCC TCTCATGCGT TGGGTCCACT CAGTAGATGC CTGTTGAATT GGGTACGCGG 
     4441 CCAGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG 
     4501 GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA 
     4561 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG 
     4621 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC 
     4681 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC 
     4741 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC 
     4801 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG 
     4861 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC 
     4921 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA 
     4981 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC 
     5041 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC 
     5101 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG 
     5161 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC 
     5221 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA 
     5281 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAACCTGAGG CTATGGCAGG 
     5341 GCCTGCCGCC CCGACGTTGG CTGCGAGCCC TGGGCCTTCA CCCGAACTTG GGGGGTGGGG 
     5401 TGGGGAAAAG GAAGAAACGC GGGCGTATTG GCCCCAATGG GGTCTCGGTG GGGTATCGAC 
     5461 AGAGTGCCAG CCCTGGGACC GAACCCCGCG TTTATGAACA AACGACCCAA CACCGTGCGT 
     5521 TTTATTCTGT CTTTTTATTG CCGTCATAGC GCGGGTTCCT TCCGGTATTG TCTCCTTCCG 
     5581 TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC GAAGAACTCC AGCATGAGAT CCCCGCGCTG 
     5641 GAGGATCATC CAGCCGGCGT CCCGGAAAAC GATTCCGAAG CCCAACCTTT CATAGAAGGC 
     5701 GGCGGTGGAA TCGAAATCTC GTGATGGCAG GTTGGGCGTC GCTTGGTCGG TCATTTCGAA 
     5761 CCCCAGAGTC CCGCTCAGAA GAACTCGTCA AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA 
     5821 TCGGGAGCGG CGATACCGTA AAGCACGAGG AAGCGGTCAG CCCATTCGCC GCCAAGCTCT 
     5881 TCAGCAATAT CACGGGTAGC CAACGCTATG TCCTGATAGC GATCCGCCAC ACCCAGCCGG 
     5941 CCACAGTCGA TGAATCCAGA AAAGCGGCCA TTTTCCACCA TGATATTCGG CAAGCAGGCA 
     6001 TCGCCATGGG TCACGACGAG ATCCTCGCCG TCGGGCATGC TCGCCTTGAG CCTGGCGAAC 
     6061 AGTTCGGCTG GCGCGAGCCC CTGATGCTCT TCGTCCAGAT CATCCTGATC GACAAGACCG 
     6121 GCTTCCATCC GAGTACGTGC TCGCTCGATG CGATGTTTCG CTTGGTGGTC GAATGGGCAG 
     6181 GTAGCCGGAT CAAGCGTATG CAGCCGCCGC ATTGCATCAG CCATGATGGA TACTTTCTCG 
     6241 GCAGGAGCAA GGTGAGATGA CAGGAGATCC TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG 
     6301 TCCCTTCCCG CTTCAGTGAC AACGTCGAGC ACAGCTGCGC AAGGAACGCC CGTCGTGGCC 
     6361 AGCCACGATA GCCGCGCTGC CTCGTCTTGC AGTTCATTCA GGGCACCGGA CAGGTCGGTC 
     6421 TTGACAAAAA GAACCGGGCG CCCCTGCGCT GACAGCCGGA ACACGGCGGC ATCAGAGCAG 
     6481 CCGATTGTCT GTTGTGCCCA GTCATAGCCG AATAGCCTCT CCACCCAAGC GGCCGGAGAA 
     6541 CCTGCGTGCA ATCCATCTTG TTCAATCATG CGAAACGATC CTCATCCTGT CTCTTGATCG 
     6601 ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA GCCTCCTCAC TACTTCTGGA ATAGCTCAGA 
     6661 GGCCGAGGCG GCCTCGGCCT CTGCATAAAT AAAAAAAATT AGTCAGCCAT GGGGCGGAGA 
     6721 ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG GATGGGCGGA GTTAGGGGCG GGACTATGGT 
     6781 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     6841 TCCACACCTG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     6901 GCCTGGGGAC TTTCCACACC CTAACTGACA CACATTCCAC AGCTGGTTCT TTCCGCCTCA 
     6961 GGACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA 
     7021 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA 
     7081 AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG 
     7141 CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT 
     7201 TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA 
     7261 GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT 
     7321 TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG 
     7381 TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT 
     7441 TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT 
     7501 TAACAAAAAT TTAACGCGAA TTTT
//