LOCUS       RC230555L1                9003 bp    DNA     circular  SYN 
DEFINITION  KDM1A (Myc-DDK-tagged)-Human lysine (K)-specific demethylase 1A (KDM1A), transcript variant 1
ACCESSION   NM_001009999
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    7294..7474 
                     /label="3'LTR"  
     misc_feature    833..1013 
                     /label="5'LTR"  
     misc_feature    8202..8861 
                     /label="CAT(Cam-r)"  
     misc_feature    2620..3234 
                     /label="CMV promoter"  
     misc_feature    7633..8201 
                     /label="ColE1"  
     misc_feature    6169..6189 
                     /label="LR50 primer"  
     misc_feature    5966..6037 
                     /label="Myc-DDK"  
     misc_feature    3252..3271 
                     /label="T7 promoter"  
     misc_feature    3221..3237 
                     /label="V2 primer"  
     misc_feature    6178..6769 
                     /label="WPRE"  
     misc_feature    3320..3328 
                     /label="Restriction Enzyme"  
     misc_feature    5948..5954 
                     /label="Restriction Enzyme"  
     misc_feature    3320..5948 
                     /label="KDM1A"  
ORIGIN                      
        1 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG 
       61 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT 
      121 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC 
      181 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT CGCGTTGACA 
      241 TTGATTATTG ACTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA 
      301 TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA 
      361 CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT 
      421 CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT 
      481 GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA 
      541 TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT 
      601 CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC AATGGGCGTG GATAGCGGTT 
      661 TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT TGTTTTGGCA 
      721 CCAAAATCAA CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA CGCAAATGGG 
      781 CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGCGCG TTTTGCCTGT ACTGGGTCTC 
      841 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 
      901 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 
      961 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 
     1021 CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG 
     1081 GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT 
     1141 TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG 
     1201 GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA 
     1261 ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT 
     1321 GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC 
     1381 AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA 
     1441 AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA 
     1501 AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT 
     1561 ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA 
     1621 GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA 
     1681 ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA 
     1741 ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT 
     1801 TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG 
     1861 CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG 
     1921 ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG 
     1981 AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA 
     2041 ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA 
     2101 AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC 
     2161 ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT 
     2221 TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA 
     2281 TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA 
     2341 GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCGGCACTG 
     2401 CGTGCGCCAA TTCTGCAGAC AAATGGCAGT ATTCATCCAC AATTTTAAAA GAAAAGGGGG 
     2461 GATTGGGGGG TACAGTGCAG GGGAAAGAAT AGTAGACATA ATAGCAACAG ACATACAAAC 
     2521 TAAAGAATTA CAAAAACAAA TTACAAAAAT TCAAAATTTT CGGGTTTATT ACAGGGACAG 
     2581 CAGAGATCCA GTTTGGTTAG TACCGGGCCC GCTCTAGACA TGTCCAATAT GACCGCCATG 
     2641 TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG 
     2701 CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC 
     2761 CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG 
     2821 GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA 
     2881 TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC 
     2941 CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT 
     3001 ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA 
     3061 GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT 
     3121 TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA 
     3181 AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG 
     3241 TCAGAATTTT GTAATACGAC TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT 
     3301 ACCGAGGAGA TCTGCCGCCG CGATCGCCAT GTTATCTGGG AAGAAGGCGG CAGCCGCGGC 
     3361 GGCGGCGGCT GCAGCGGCAG CAACCGGGAC GGAGGCTGGC CCTGGGACAG CAGGCGGCTC 
     3421 CGAGAACGGG TCTGAGGTGG CCGCGCAGCC CGCGGGCCTG TCGGGCCCAG CCGAGGTCGG 
     3481 GCCGGGGGCG GTGGGGGAGC GCACACCCCG CAAGAAAGAG CCTCCGCGGG CCTCGCCCCC 
     3541 CGGGGGCCTG GCGGAACCGC CGGGGTCCGC AGGGCCTCAG GCCGGCCCTA CTGTCGTGCC 
     3601 TGGGTCTGCG ACCCCCATGG AAACTGGAAT AGCAGAGACT CCGGAGGGGC GTCGGACCAG 
     3661 CCGGCGCAAG CGGGCGAAGG TAGAGTACAG AGAGATGGAT GAAAGCTTGG CCAACCTCTC 
     3721 AGAAGATGAG TATTATTCAG AAGAAGAGAG AAATGCCAAA GCAGAGAAGG AAAAGAAGCT 
     3781 TCCCCCACCA CCCCCTCAAG CCCCACCTGA GGAAGAAAAT GAAAGTGAGC CTGAAGAACC 
     3841 ATCGGGGCAA GCAGGAGGAC TTCAAGACGA CAGTTCTGGA GGGTATGGAG ACGGCCAAGC 
     3901 ATCAGGTGTG GAGGGCGCAG CTTTCCAGAG CCGACTTCCT CATGACCGGA TGACTTCTCA 
     3961 AGAAGCAGCC TGTTTTCCAG ATATTATCAG TGGACCACAA CAGACCCAGA AGGTTTTTCT 
     4021 TTTCATTAGA AACCGCACAC TGCAGTTGTG GTTGGATAAT CCAAAGATTC AGCTGACATT 
     4081 TGAGGCTACT CTCCAACAAT TAGAAGCACC TTATAACAGT GATACTGTGC TTGTCCACCG 
     4141 AGTTCACAGT TATTTAGAGC GTCATGGTCT TATCAACTTC GGCATCTATA AGAGGATAAA 
     4201 ACCCCTACCA ACTAAAAAGA CAGGAAAGGT AATTATTATA GGCTCTGGGG TCTCAGGCTT 
     4261 GGCAGCAGCT CGACAGTTAC AAAGTTTTGG AATGGATGTC ACACTTTTGG AAGCCAGGGA 
     4321 TCGTGTGGGT GGACGAGTTG CCACATTTCG CAAAGGAAAC TATGTAGCTG ATCTTGGAGC 
     4381 CATGGTGGTA ACAGGTCTTG GAGGGAATCC TATGGCTGTG GTCAGCAAAC AAGTAAATAT 
     4441 GGAACTGGCC AAGATCAAGC AAAAATGCCC ACTTTATGAA GCCAACGGAC AAGCTGACAC 
     4501 TGTCAAGGTT CCTAAAGAGA AAGATGAAAT GGTAGAGCAA GAGTTTAACC GGTTGCTAGA 
     4561 AGCTACATCT TACCTTAGTC ATCAACTAGA CTTCAATGTC CTCAATAATA AGCCTGTGTC 
     4621 CCTTGGCCAG GCATTGGAAG TTGTCATTCA GTTACAAGAG AAGCATGTCA AAGATGAGCA 
     4681 GATTGAACAT TGGAAGAAGA TAGTGAAAAC TCAGGAAGAA TTGAAAGAAC TTCTTAATAA 
     4741 GATGGTAAAT TTGAAAGAGA AAATTAAAGA ACTCCATCAG CAATACAAAG AAGCATCTGA 
     4801 AGTAAAGCCA CCCAGAGATA TTACTGCCGA GTTCTTAGTG AAAAGCAAAC ACAGGGATCT 
     4861 GACCGCCCTA TGCAAGGAAT ATGATGAATT AGCTGAAACA CAAGGAAAGC TAGAAGAAAA 
     4921 ACTTCAGGAG TTGGAAGCGA ATCCCCCAAG TGATGTATAT CTCTCATCAA GAGACAGACA 
     4981 AATACTTGAT TGGCATTTTG CAAATCTTGA ATTTGCTAAT GCCACACCTC TCTCAACTCT 
     5041 CTCCCTTAAG CACTGGGATC AGGATGATGA CTTTGAGTTC ACTGGCAGCC ACCTGACAGT 
     5101 AAGGAATGGC TACTCGTGTG TGCCTGTGGC TTTAGCAGAA GGCCTAGACA TTAAACTGAA 
     5161 TACAGCAGTG CGACAGGTTC GCTACACGGC TTCAGGATGT GAAGTGATAG CTGTGAATAC 
     5221 CCGCTCCACG AGTCAAACCT TTATTTATAA ATGCGACGCA GTTCTCTGTA CCCTTCCCCT 
     5281 GGGTGTGCTG AAGCAGCAGC CACCAGCCGT TCAGTTTGTG CCACCTCTCC CTGAGTGGAA 
     5341 AACATCTGCA GTCCAAAGGA TGGGATTTGG CAACCTTAAC AAGGTGGTGT TGTGTTTTGA 
     5401 TCGGGTGTTC TGGGATCCAA GTGTCAATTT GTTCGGGCAT GTTGGCAGTA CGACTGCCAG 
     5461 CAGGGGTGAG CTCTTCCTCT TCTGGAACCT CTATAAAGCT CCAATACTGT TGGCACTAGT 
     5521 GGCAGGAGAA GCTGCTGGTA TCATGGAAAA CATAAGTGAC GATGTGATTG TTGGCCGATG 
     5581 CCTGGCCATT CTCAAAGGGA TTTTTGGTAG CAGTGCAGTA CCTCAGCCCA AAGAAACTGT 
     5641 GGTGTCTCGT TGGCGTGCTG ATCCCTGGGC TCGGGGCTCT TATTCCTATG TTGCTGCAGG 
     5701 ATCATCTGGA AATGACTATG ATTTAATGGC TCAGCCAATC ACTCCTGGCC CCTCGATTCC 
     5761 AGGTGCCCCA CAGCCGATTC CACGACTCTT CTTTGCGGGA GAACATACGA TCCGTAACTA 
     5821 CCCAGCCACA GTGCATGGTG CTCTGCTGAG TGGGCTGCGA GAAGCGGGAA GAATTGCAGA 
     5881 CCAGTTTTTG GGGGCCATGT ATACGCTGCC TCGCCAGGCC ACACCAGGTG TTCCTGCACA 
     5941 GCAGTCCCCA AGCATGACGC GTACGCGGCC GCTCGAGCAG AAACTCATCT CAGAAGAGGA 
     6001 TCTGGCAGCA AATGATATCC TGGATTACAA GGATGACGAC GATAAGGTTT AAACGGCCGG 
     6061 CCGCGGTCTG TACAAGTAGG ATTCGTCGAG GGACCTAATA ACTTCGTATA GCATACATTA 
     6121 TACGAAGTTA TACATGTTTA AGGGTTCCGG TTCCACTAGG TACAATTCGA TATCAAGCTT 
     6181 ATCGATAATC AACCTCTGGA TTACAAAATT TGTGAAAGAT TGACTGGTAT TCTTAACTAT 
     6241 GTTGCTCCTT TTACGCTATG TGGATACGCT GCTTTAATGC CTTTGTATCA TGCTATTGCT 
     6301 TCCCGTATGG CTTTCATTTT CTCCTCCTTG TATAAATCCT GGTTGCTGTC TCTTTATGAG 
     6361 GAGTTGTGGC CCGTTGTCAG GCAACGTGGC GTGGTGTGCA CTGTGTTTGC TGACGCAACC 
     6421 CCCACTGGTT GGGGCATTGC CACCACCTGT CAGCTCCTTT CCGGGACTTT CGCTTTCCCC 
     6481 CTCCCTATTG CCACGGCGGA ACTCATCGCC GCCTGCCTTG CCCGCTGCTG GACAGGGGCT 
     6541 CGGCTGTTGG GCACTGACAA TTCCGTGGTG TTGTCGGGGA AATCATCGTC CTTTCCTTGG 
     6601 CTGCTCGCCT GTGTTGCCAC CTGGATTCTG CGCGGGACGT CCTTCTGCTA CGTCCCTTCG 
     6661 GCCCTCAATC CAGCGGACCT TCCTTCCCGC GGCCTGCTGC CGGCTCTGCG GCCTCTTCCG 
     6721 CGTCTTCGCC TTCGCCCTCA GACGAGTCGG ATCTCCCTTT GGGCCGCCTC CCCGCATCGA 
     6781 TACCGTCGAC CTCGATCGAG ACCTAGAAAA ACATGGAGCA ATCACAAGTA GCAATACAGC 
     6841 AGCTACCAAT GCTGATTGTG CCTGGCTAGA AGCACAAGAG GAGGAGGAGG TGGGTTTTCC 
     6901 AGTCACACCT CAGGTACCTT TAAGACCAAT GACTTACAAG GCAGCTGTAG ATCTTAGCCA 
     6961 CTTTTTAAAA GAAAAGGGGG GACTGGAAGG GCTAATTCAC TCCCAACGAA GACAAGATAT 
     7021 CCTTGATCTG TGGATCTACC ACACACAAGG CTACTTCCCT GATTGGCAGA ACTACACACC 
     7081 AGGGCCAGGG ATCAGATATC CACTGACCTT TGGATGGTGC TACAAGCTAG TACCAGTTGA 
     7141 GCAAGAGAAG GTAGAAGAAG CCAATGAAGG AGAGAACACC CGCTTGTTAC ACCCTGTGAG 
     7201 CCTGCATGGG ATGGATGACC CGGAGAGAGA AGTATTAGAG TGGAGGTTTG ACAGCCGCCT 
     7261 AGCATTTCAT CACATGGCCC GAGAGCTGCA TCCGGACTGT ACTGGGTCTC TCTGGTTAGA 
     7321 CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA AGCCTCAATA 
     7381 AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT CTGGTAACTA 
     7441 GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGCATGT GAGCAAAAGG 
     7501 CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG 
     7561 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG 
     7621 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC 
     7681 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA 
     7741 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT 
     7801 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC 
     7861 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG 
     7921 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC 
     7981 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT 
     8041 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA 
     8101 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG 
     8161 GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA TTACGCCCCG 
     8221 CCCTGCCACT CATCGCAGTA CTGTTGTAAT TCATTAAGCA TTCTGCCGAC ATGGAAGCCA 
     8281 TCACAAACGG CATGATGAAC CTGAATCGCC AGCGGCATCA GCACCTTGTC GCCTTGCGTA 
     8341 TAATATTTGC CCATGGTGAA AACGGGGGCG AAGAAGTTGT CCATATTGGC CACGTTTAAA 
     8401 TCAAAACTGG TGAAACTCAC CCAGGGATTG GCTGAGACGA AAAACATATT CTCAATAAAC 
     8461 CCTTTAGGGA AATAGGCCAG GTTTTCACCG TAACACGCCA CATCTTGCGA ATATATGTGT 
     8521 AGAAACTGCC GGAAATCGTC GTGGTATTCA CTCCAGAGCG ATGAAAACGT TTCAGTTTGC 
     8581 TCATGGAAAA CGGTGTAACA AGGGTGAACA CTATCCCATA TCACCAGCTC ACCGTCTTTC 
     8641 ATTGCCATAC GGAACTCCGG ATGAGCATTC ATCAGGCGGG CAAGAATGTG AATAAAGGCC 
     8701 GGATAAAACT TGTGCTTATT TTTCTTTACG GTCTTTAAAA AGGCCGTAAT ATCCAGCTGA 
     8761 ACGGTCTGGT TATAGGTACA TTGAGCAACT GACTGAAATG CCTCAAAATG TTCTTTACGA 
     8821 TGCCATTGGG ATATATCAAC GGTGGTATAT CCAGTGATTT TTTTCTCCAT ACTCTTCCTT 
     8881 TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA 
     8941 TGTATTTAGA AAAATAAACA AATAGGGGTC CCGCGCACAT TTCCCCGAAA AGTGCCACCT 
     9001 GAC
//