LOCUS       RC229441                9267 bp    DNA     circular  SYN 
DEFINITION  FGD5 (Myc-DDK-tagged)-Human FYVE, RhoGEF and PH domain containing 5 (FGD5)
ACCESSION   NM_152536
VERSION     NM_152536.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5424..5515 
                     /label="Myc-DDK"  
     misc_feature    5545..6130 
                     /label="PolyA signal"  
     misc_feature    5602..5621 
                     /label="Restriction Enzyme"  
     misc_feature    6406..7078 
                     /label="ColE1"  
     misc_feature    7508..8302 
                     /label="Kan/Neo"  
     misc_feature    8337..8639 
                     /label="SV40 ori"  
     misc_feature    8725..9202 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5406..5412 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5406 
                     /label="FGD5"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTTCAGGGGT CCGAAGCCCC CCATTGCCCC CAAGCCCAGG CTGACTGCCC 
     1081 CAAACGAGTG GAGAGCCAGT GTGTACCTGA ATGACAGCTT GAACAAATGC AGCAACGGGC 
     1141 GGCTGCCCTG TGTAGACAGG GGGCTTGATG AGGGGCCCCG GTCCATCCCA AAGTGCTCTG 
     1201 AGTCGGAGAC CGACGAGGAT TACATCGTGG TCCCCAGGGT TCCGCTGAGG GAGGATGAAC 
     1261 CCAAGGACGA GGGCAGTGTG GGGAACAAAG CCCTGGTGTC TCCCGAGTCC TCTGCGGAAG 
     1321 AGGAAGAGGA GCGTGAAGAG GGAGGCGAGG CATGTGGCCT GGAGGGTACA GGAGCTGGTG 
     1381 AGGATTCAGT GGCCCCTGCT GCTCCGGGTG CAGGAGCGCT GAGCAGGGAG GGTGAGGAAG 
     1441 GCACAGACCT TGCTCTTGAG GATGAAGGGG AGGGCTGCGC TGATGAGCCA GGGACACTGG 
     1501 AGCAGGTGTC CAGAAGTGAG GAGGAAGAGA AGCTAGTGCA GCCACACAGG GAGTGCAGCC 
     1561 TGGAGGACAG TGGGCCTTGG GCTGGAGAGG GGGTCTTCCA GAGCGACCTC CTCCTGCCTC 
     1621 ACATCCATGG AGAGGACCAG GAGCCCCCCG ACACCCCCGG GGAGGCAGAG GAGGATGATG 
     1681 AGGAAGGCTG TGCCAGCACA GACCCAGCAG GGGCAGATGA GGGTTCGGGT CCTGACAGGC 
     1741 CCACGGAGGA CATGGGACAG GATGCTGAGG ACACCAGTGA GGAGCCCCCT GAGAAGGAGG 
     1801 AGCTGGCCGG GGTCCAGGAG GCAGAGACAG CCACAGACTG CCCTGAAGTT CTTGAGGAGG 
     1861 GATGTGAAGA GGCCACGGGT GTCACAGGTG GGGAACAGGT TGACCTCAGT GAACCACCTG 
     1921 ACCACGAGAA GAAAACCAAC CAAGAAGTGG CAGCCGCCAC CCTGGAGGAC CATGCACAGG 
     1981 ATGAGTCCGC CGAGGAGAGC TGCCAGATTG TCCCTTTTGA GAATGACTGC ATGGAGGACT 
     2041 TCGTGACTTC CCTCACAGGA AGCCCCTATG AGTTCTTCCC AACTGAGAGC ACCTCTTTTT 
     2101 GCAGCGAGAG CTGTTCTCCT CTTTCTGAAT CAGCGAAAGG TTTAGAATCA GAGCAGGCAC 
     2161 CAAAGCTGGG GCTGCGTGCG GAGGAGAACC CCATGGTGGG GGCTTTGTGT GGCCAGTGTG 
     2221 GCTCCCTACA GGGTGGAGCG GCCGAGGGTC CCGCAGCCCC TGATGTGGTG GTCGTGCTGG 
     2281 AGGAGGAGGC CTTGGATGAT GCACTGGCCA ACCCCTATGT GATGGGAGTG GGCCTGCCCG 
     2341 GTCAGGCGGC CCCTGGAGAA GGAGGGCAGG CTGCATCGGA CGCCCTGGGT GGTTATGGCT 
     2401 CGAAAGAAGA ATTGAACTGT GAGGCAGAGG GTGGCCTGGT TCCCGCGGAC AGGAAGAACA 
     2461 CCAGCACGAG GGTCCGGCCC CACTCTGGGA AGGTGGCCGG CTATGTCCCA GAAACCGTCC 
     2521 CTGAAGAAAC CGGACCTGAG GCGGGCTCGT CAGCCCCTGG CATTGGAGGT GCCGCAGAGG 
     2581 AGGTGGGAAA GACGCTTTTG TCATTGGAGG GGAAGCCCTT GGAAGCCAGC AGGGCCTTGC 
     2641 CAGCAAAGCC CAGGGCCTTT ACTTTATACC CTCGGTCGTT CTCCGTGGAA GGCCGAGAGA 
     2701 TTCCAGTGTC CGTGTACCAG GAGCCTGAGG GGTCAGGGTT GGATGACCAC AGGATAAAGA 
     2761 GGAAAGAGGA CAATCTCTCT CTGTCGTGTG TAATTGGCTC CTCTGGGAGT TTCTCCCAGA 
     2821 GAAACCACCT TCCGTCCAGC GGCACCTCCA CGCCTTCTTC CATGGTCGAC ATCCCACCTC 
     2881 CTTTCGACCT GGCCTGCATC ACCAAGAAGC CCATCACAAA GAGCTCTCCC TCACTCCTGA 
     2941 TCGAGAGCGA CTCCCCGGAC AAGTACAAGA AGAAGAAGTC ATCCTTTAAG CGCTTCCTGG 
     3001 CACTGACGTT TAAGAAGAAG ACGGAGAACA AATTGCATGT GGATGTGAAC GTGTCTTCCT 
     3061 CTAGGTCCTC TTCAGAGTCC AGCTACCACG GGCCTTCCAG GATTCTGGAA GTTGACCGGA 
     3121 GAAGCCTCAG CAACTCCCCT CAGCTTAAGT CTCGGACTGG GAAGCTCCGG GCTTCTGAAT 
     3181 CCCCCTCCTC CCTCATCTTT TATAGAGATG GCAAGAGGAA AGGTGTCCCC TTCAGCAGGA 
     3241 CGGTGTCCAG AGTGGAGTCC TTTGAAGACC GCTCCCGGCC GCCCTTCCTG CCCTTGCCAC 
     3301 TGACCAAGCC ACGGTCCATC TCCTTCCCCA GCGCTGACAC TTCAGACTAT GAGAACATTC 
     3361 CAGCCATGAA CTCGGACTAT GAGAATATCC AGATTCCACC CCGGAGACCT GCCAGGGCTG 
     3421 GCGCGTTCAC GAAGCTGTTT GAAGATCAGA GCAGAGCCCT GTCCACAGCA AACGAAAATG 
     3481 ATGGCTACGT GGACATGAGC AGCTTCAACG CCTTTGAGAG CAAACAGCAG AGTGCAGACC 
     3541 AGGACGCAGA AAGCGCCTAC ACAGAGCCCT ACAAAGTCTG TCCCATCTCG TCGGCAGCCC 
     3601 CCAAAGAGGA CCTTACGTCG GATGAAGAGC AGAGAAGCTC GGAGGAGGAG GACAGTGCTT 
     3661 CAAGAGACCC CAGTGTCACC CACAAGGTGG AAGGACAGTC CAGAGCCCTT GTCATCGCAC 
     3721 AGGAACTGCT ATCTTCAGAG AAAGCATACG TGGAGATGCT CCAGCACTTA AATCTGGATT 
     3781 TCCATGGAGC TGTCATGAGG GCCTTGGATG ACATGGACCA TGAAGGCAGA GACACATTGG 
     3841 CCCGGGAGGA GCTGAGGCAG GGCCTGAGTG AACTCCCAGC CATCCACGAC CTTCATCAAG 
     3901 GCATCCTGGA GGAGCTGGAG GAAAGGCTGT CAAATTGGGA GAGCCAGCAG AAGGTAGCTG 
     3961 ACGTCTTCCT GGCCCGGGAG CAGGGGTTTG ATCACCACGC CACTCACATC CTGCAGTTCG 
     4021 ACAGGTACCT AGGTCTGCTC AGTGAGAATT GCCTCCACTC TCCCCGGCTG GCAGCTGCTG 
     4081 TCCGTGAATT TGAGCAGAGT GTACAAGGAG GCAGCCAGAC TGCGAAGCAT CGGCTGCTGC 
     4141 GGGTGGTTCA ACGCCTCTTC CAGTACCAAG TGCTCCTCAC AGACTATTTA AACAACCTTT 
     4201 GTCCGGACTC CGCCGAGTAC GACAACACAC AGGGTGCACT GAGCCTCATC TCCAAAGTCA 
     4261 CAGACCGTGC CAACGACAGC ATGGAGCAAG GGGAAAACCT GCAGAAGCTG GTCCACATTG 
     4321 AGCACAGCGT CCGGGGCCAA GGGGATCTCC TCCAGCCAGG AAGGGAGTTT CTGAAGGAAG 
     4381 GGACGCTGAT GAAAGTAACA GGGAAAAACA GACGGCCCCG GCACCTATTT CTGATGAACG 
     4441 ATGTGCTCCT GTACACCTAT CCCCAGAAGG ATGGGAAGTA CCGGCTGAAG AACACATTGG 
     4501 CTGTGGCCAA CATGAAGGTC AGCCGCCCTG TGATGGAGAA AGTGCCCTAC GCTCTAAAGA 
     4561 TTGAGACTTC CGAGTCCTGC CTGATGCTGT CTGCGAGCTC CTGTGCAGAG AGGGACGAGT 
     4621 GGTATGGCTG TCTGAGCAGA GCCCTCCCTG AGGACTACAA GGCCCAGGCG CTGGCTGCAT 
     4681 TCCACCATAG CGTGGAGATA CGAGAGAGGC TGGGGGTTAG CCTTGGGGAG AGGCCCCCCA 
     4741 CCCTGGTGCC TGTCACACAC GTCATGATGT GCATGAACTG CGGCTGCGAC TTCTCCCTCA 
     4801 CCCTGCGGCG TCATCACTGT CACGCCTGTG GCAAGATCGT GTGCCGGAAC TGTTCGCGGA 
     4861 ACAAGTACCC GCTGAAGTAC CTGAAGGACA GGATGGCCAA GGTCTGCGAC GGCTGCTTCG 
     4921 GGGAGCTGAA GAAGCGGGGC AGGGCTGTCC CGGGCCTGAT GAGAGAGCGG CCTGTGAGCA 
     4981 TGAGCTTCCC GCTGTCTTCA CCCCGCTTCT CGGGCAGTGC CTTTTCATCC GTCTTCCAGA 
     5041 GCATTAACCC CTCGACCTTC AAGAAGCAGA AGAAAGTCCC TTCAGCCCTG ACAGAGGTGG 
     5101 CTGCCTCTGG AGAGGGCTCT GCCATCAGTG GCTATCTCAG CCGGTGTAAG AGGGGCAAGC 
     5161 GGCACTGGAA GAAGCTCTGG TTTGTCATCA AAGGCAAAGT TCTCTACACC TACATGGCCA 
     5221 GTGAGGACAA AGTGGCCTTG GAGAGTATGC CTCTGCTAGG CTTCACCATT GCTCCAGAAA 
     5281 AGGAAGAGGG CAGCAGTGAA GTAGGACCTA TTTTTCACCT TTACCACAAG AAAACCCTAT 
     5341 TTTATAGCTT CAAAGCAGAA GATACCAATT CAGCTCAGAG GTGGATCGAG GCCATGGAAG 
     5401 ATGCGAGTGT GTTAACGCGT ACGCGGCCGC TCGAGCAGAA ACTCATCTCA GAAGAGGATC 
     5461 TGGCAGCAAA TGATATCCTG GATTACAAGG ATGACGACGA TAAGGTTTAA ACGGCCGGCC 
     5521 GCGGTCATAG CTGTTTCCTG AACAGATCCC GGGTGGCATC CCTGTGACCC CTCCCCAGTG 
     5581 CCTCTCCTGG CCCTGGAAGT TGCCACTCCA GTGCCCACCA GCCTTGTCCT AATAAAATTA 
     5641 AGTTGCATCA TTTTGTCTGA CTAGGTGTCC TTCTATAATA TTATGGGGTG GAGGGGGGTG 
     5701 GTATGGAGCA AGGGGCAAGT TGGGAAGACA ACCTGTAGGG CCTGCGGGGT CTATTGGGAA 
     5761 CCAAGCTGGA GTGCAGTGGC ACAATCTTGG CTCACTGCAA TCTCCGCCTC CTGGGTTCAA 
     5821 GCGATTCTCC TGCCTCAGCC TCCCGAGTTG TTGGGATTCC AGGCATGCAT GACCAGGCTC 
     5881 AGCTAATTTT TGTTTTTTTG GTAGAGACGG GGTTTCACCA TATTGGCCAG GCTGGTCTCC 
     5941 AACTCCTAAT CTCAGGTGAT CTACCCACCT TGGCCTCCCA AATTGCTGGG ATTACAGGCG 
     6001 TGAACCACTG CTCCCTTCCC TGTCCTTCTG ATTTTAAAAT AACTATACCA GCAGGAGGAC 
     6061 GTCCAGACAC AGCATAGGCT ACCTGGCCAT GCCCAACCGG TGGGACATTT GAGTTGCTTG 
     6121 CTTGGCACTG TCCTCTCATG CGTTGGGTCC ACTCAGTAGA TGCCTGTTGA ATTGGGTACG 
     6181 CGGCCAGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC 
     6241 AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA 
     6301 AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA 
     6361 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC 
     6421 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC 
     6481 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG 
     6541 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA 
     6601 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC 
     6661 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC 
     6721 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG 
     6781 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA 
     6841 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA 
     6901 AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA 
     6961 CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT 
     7021 AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAACCTG AGGCTATGGC 
     7081 AGGGCCTGCC GCCCCGACGT TGGCTGCGAG CCCTGGGCCT TCACCCGAAC TTGGGGGGTG 
     7141 GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA TTGGCCCCAA TGGGGTCTCG GTGGGGTATC 
     7201 GACAGAGTGC CAGCCCTGGG ACCGAACCCC GCGTTTATGA ACAAACGACC CAACACCGTG 
     7261 CGTTTTATTC TGTCTTTTTA TTGCCGTCAT AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT 
     7321 CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG GGCGAAGAAC TCCAGCATGA GATCCCCGCG 
     7381 CTGGAGGATC ATCCAGCCGG CGTCCCGGAA AACGATTCCG AAGCCCAACC TTTCATAGAA 
     7441 GGCGGCGGTG GAATCGAAAT CTCGTGATGG CAGGTTGGGC GTCGCTTGGT CGGTCATTTC 
     7501 GAACCCCAGA GTCCCGCTCA GAAGAACTCG TCAAGAAGGC GATAGAAGGC GATGCGCTGC 
     7561 GAATCGGGAG CGGCGATACC GTAAAGCACG AGGAAGCGGT CAGCCCATTC GCCGCCAAGC 
     7621 TCTTCAGCAA TATCACGGGT AGCCAACGCT ATGTCCTGAT AGCGATCCGC CACACCCAGC 
     7681 CGGCCACAGT CGATGAATCC AGAAAAGCGG CCATTTTCCA CCATGATATT CGGCAAGCAG 
     7741 GCATCGCCAT GGGTCACGAC GAGATCCTCG CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG 
     7801 AACAGTTCGG CTGGCGCGAG CCCCTGATGC TCTTCGTCCA GATCATCCTG ATCGACAAGA 
     7861 CCGGCTTCCA TCCGAGTACG TGCTCGCTCG ATGCGATGTT TCGCTTGGTG GTCGAATGGG 
     7921 CAGGTAGCCG GATCAAGCGT ATGCAGCCGC CGCATTGCAT CAGCCATGAT GGATACTTTC 
     7981 TCGGCAGGAG CAAGGTGAGA TGACAGGAGA TCCTGCCCCG GCACTTCGCC CAATAGCAGC 
     8041 CAGTCCCTTC CCGCTTCAGT GACAACGTCG AGCACAGCTG CGCAAGGAAC GCCCGTCGTG 
     8101 GCCAGCCACG ATAGCCGCGC TGCCTCGTCT TGCAGTTCAT TCAGGGCACC GGACAGGTCG 
     8161 GTCTTGACAA AAAGAACCGG GCGCCCCTGC GCTGACAGCC GGAACACGGC GGCATCAGAG 
     8221 CAGCCGATTG TCTGTTGTGC CCAGTCATAG CCGAATAGCC TCTCCACCCA AGCGGCCGGA 
     8281 GAACCTGCGT GCAATCCATC TTGTTCAATC ATGCGAAACG ATCCTCATCC TGTCTCTTGA 
     8341 TCGATCTTTG CAAAAGCCTA GGCCTCCAAA AAAGCCTCCT CACTACTTCT GGAATAGCTC 
     8401 AGAGGCCGAG GCGGCCTCGG CCTCTGCATA AATAAAAAAA ATTAGTCAGC CATGGGGCGG 
     8461 AGAATGGGCG GAACTGGGCG GAGTTAGGGG CGGGATGGGC GGAGTTAGGG GCGGGACTAT 
     8521 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     8581 CTTTCCACAC CTGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     8641 GGAGCCTGGG GACTTTCCAC ACCCTAACTG ACACACATTC CACAGCTGGT TCTTTCCGCC 
     8701 TCAGGACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC 
     8761 GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC 
     8821 CGAAAAGTGC CACCTGACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT 
     8881 ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC 
     8941 CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT 
     9001 TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT 
     9061 GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC 
     9121 ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC 
     9181 TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG 
     9241 ATTTAACAAA AATTTAACGC GAATTTT
//