LOCUS       RC229439                9081 bp    DNA     circular  SYN 
DEFINITION  SCAPER (Myc-DDK-tagged)-Human S-phase cyclin A-associated protein in the ER (SCAPER), transcript variant 1
ACCESSION   NM_020843
VERSION     NM_020843.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5238..5329 
                     /label="Myc-DDK"  
     misc_feature    5359..5944 
                     /label="PolyA signal"  
     misc_feature    5416..5435 
                     /label="Restriction Enzyme"  
     misc_feature    6220..6892 
                     /label="ColE1"  
     misc_feature    7322..8116 
                     /label="Kan/Neo"  
     misc_feature    8151..8453 
                     /label="SV40 ori"  
     misc_feature    8539..9016 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5220..5226 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5220 
                     /label="SCAPER"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GATGGCTTCA TTCCAGCGCT CCAATAGTCA TGACAAAGTA AGGAGAATAG 
     1081 TTGCAGAGGA GGGTCGTACA GCAAGAAACC TAATAGCTTG GAGTGTTCCA CTAGAAAGCA 
     1141 AAGATGATGA TGGAAAACCT AAATGTCAAA CTGGTGGAAA ATCTAAGAGG ACCATTCAAG 
     1201 GCACTCATAA AACTACTAAA CAGAGTACTG CAGTGGACTG TAAAATAACA TCGTCTACGA 
     1261 CTGGAGATAA ACACTTTGAT AAAAGTCCCA CTAAAACAAG GCACCCTCGG AAAATTGATC 
     1321 TAAGAGCTCG ATACTGGGCA TTTCTTTTTG ATAATCTTCG CCGAGCAGTA GATGAAATCT 
     1381 ATGTAACTTG CGAATCAGAT CAGAGTGTGG TCGAATGTAA GGAGGTGCTA ATGATGCTGG 
     1441 ATAACTATGT AAGAGATTTC AAAGCATTGA TTGACTGGAT TCAGCTTCAG GAAAAGCTAG 
     1501 AGAAGACAGA TGCTCAAAGC AGACCAACAT CATTGGCATG GGAAGTAAAG AAGATGTCTC 
     1561 CGGGACGCCA TGTGATTCCA AGTCCATCAA CAGATAGAAT AAATGTAACA TCAAATGCTC 
     1621 GACGAAGCTT AAATTTTGGA GGTTCAACTG GCACAGTGCC AGCTCCTCGT CTGGCTCCCA 
     1681 CAGGTGTCAG TTGGGCTGAC AAGGTAAAGG CTCATCATAC AGGCTCTACT GCTTCTTCAG 
     1741 AAATAACACC CGCCCAGTCT TGCCCACCAA TGACAGTGCA GAAGGCCTCA CGCAAAAATG 
     1801 AACGGAAAGA TGCTGAAGGA TGGGAGACCG TTCAGAGAGG AAGGCCTATT CGTTCTCGAT 
     1861 CAACAGCAGT GATGCCAAAA GTTTCATTGG CAACAGAAGC CACAAGATCA AAGGATGACA 
     1921 GTGATAAAGA AAATGTATGT CTTTTACCTG ATGAAAGCAT ACAGAAAGGT CAATTTGTTG 
     1981 GAGATGGAAC TTCTAATACT ATAGAATCTC ATCCCAAAGA CTCATTACAC TCTTGTGACC 
     2041 ATCCTCTTGC CGAAAAAACC CAGTTCACAG TGAGTACATT GGATGATGTG AAGAATTCTG 
     2101 GCAGTATTCG AGACAATTAT GTTCGAACTT CTGAAATATC TGCTGTCCAC ATTGATACAG 
     2161 AGTGTGTTTC AGTTATGCTG CAAGCTGGTA CACCTCCTTT ACAAGTAAAT GAAGAAAAAT 
     2221 TTCCAGCAGA GAAAGCAAGG ATAGAAAATG AAATGGACCC TTCAGATATT TCAAATTCCA 
     2281 TGGCAGAAGT CCTTGCTAAA AAAGAAGAGC TAGCAGATCG TCTAGAAAAG GCCAATGAAG 
     2341 AAGCCATTGC TAGTGCTATT GCTGAAGAAG AACAGTTAAC TAGAGAAATT GAAGCTGAAG 
     2401 AAAACAATGA TATTAACATT GAAACTGACA ACGACAGTGA TTTTTCTGCC AGCATGGGCA 
     2461 GTGGGAGTGT TTCTTTCTGT GGTATGTCCA TGGACTGGAA CGATGTCCTT GCAGATTATG 
     2521 AAGCTCGTGA GTCTTGGCGC CAAAATACAT CCTGGGGGGA CATTGTAGAA GAAGAACCTG 
     2581 CTAGACCTCC AGGGCATGGA ATTCACATGC ATGAAAAACT TTCTTCACCC TCTCGTAAAA 
     2641 GAACAATTGC AGAATCTAAG AAGAAACATG AAGAAAAACA AATGAAAGCA CAGCAGCTAA 
     2701 GGGAAAAGTT ACGCGAAGAG AAAACATTGA AGCTTCAGAA ATTGTTAGAA AGGGAGAAGG 
     2761 ATGTCCGGAA GTGGAAGGAA GAATTGCTAG ATCAACGACG CAGGATGATG GAAGAAAAAT 
     2821 TACTTCATGC TGAGTTTAAG CGAGAAGTGC AGTTACAAGC AATTGTGAAA AAAGCACAAG 
     2881 AAGAAGAAGC TAAGGTAAAT GAAATTGCCT TTATAAATAC CCTTGAAGCC CAGAATAAAC 
     2941 GTCATGATGT TTTATCAAAA TTGAAGGAAT ATGAACAGAG GCTTAATGAG CTACAGGAAG 
     3001 AGCGTCAGAG AAGACAGGAA GAAAAGCAAG CACGTGATGA AGCTGTGCAG GAACGCAAGA 
     3061 GAGCTCTAGA GGCAGAGCGG CAGGCCCGTG TAGAAGAATT GTTAATGAAG AGGAAAGAAC 
     3121 AAGAAGCCCG AATTGAACAA CAGAGGCAAG AAAAGGAAAA AGCCCGTGAG GATGCAGCCC 
     3181 GGGAAAGAGC TAGAGACAGG GAAGAACGAT TGGCAGCACT CACAGCTGCT CAACAAGAAG 
     3241 CTATGGAAGA GTTACAGAAA AAAATTCAGC TCAAGCATGA TGAAAGTATT CGAAGGCACA 
     3301 TGGAACAGAT TGAACAAAGA AAAGAAAAAG CTGCTGAGCT AAGCAGTGGG CGACATGCAA 
     3361 ATACTGATTA TGCCCCCAAA CTGACCCCTT ATGAAAGAAA GAAGCAGTGT TCTCTCTGCA 
     3421 ATGTCCTGAT CTCTTCAGAG GTATATCTTT TTAGCCATGT TAAAGGGAGA AAACACCAGC 
     3481 AAGCCGTGAG AGAGAATACC AGCATCCAGG GGCGTGAACT GTCAGATGAA GAAGTGGAGC 
     3541 ATCTTTCCTT GAAGAAGTAC ATTATTGACA TTGTGGTTGA AAGTACAGCT CCAGCAGAAG 
     3601 CTTTGAAAGA TGGAGAAGAG CGGCAAAAAA ATAAAAAAAA AGCCAAAAAG ATAAAAGCCC 
     3661 GGATGAACTT CAGGGCTAAG GAATATGAGA GTTTAATGGA AACCAAAAAT TCTGGCTCTG 
     3721 ATTCACCTTA TAAAGCAAAG CTTCAGCGAT TAGCCAAAGA TCTTCTAAAA CAAGTACAAG 
     3781 TTCAAGACAG TGGCTCATGG GCAAACAATA AAGTGTCTGC TTTGGATCGG ACCCTAGGAG 
     3841 AGATCACTAG AATACTGGAA AAAGAGAATG TGGCAGATCA GATTGCATTT CAAGCTGCTG 
     3901 GTGGATTAAC AGCCCTTGAA CACATCCTTC AAGCAGTAGT CCCAGCCACA AATGTGAACA 
     3961 CAGTTTTAAG AATTCCTCCT AAGTCTCTCT GCAATGCAAT CAATGTTTAC AACCTCACCT 
     4021 GCAATAACTG TTCAGAAAAC TGCAGTGATG TTCTGTTTAG TAACAAGATT ACCTTCTTAA 
     4081 TGGACCTCCT GATACACCAG TTGACGGTTT ATGTTCCAGA TGAAAATAAT ACTATTTTGG 
     4141 GGAGAAATAC AAATAAACAA GTTTTTGAAG GCTTGACAAC TGGACTTCTC AAAGTCAGTG 
     4201 CTGTGGTTTT GGGCTGCCTG ATTGCCAATC GACCAGATGG AAACTGCCAG CCAGCTACCC 
     4261 CAAAAATACC AACACAGGAA ATGAAAAACA AACCCTCACA AGGTGATCCT TTTAACAATC 
     4321 GAGTTCAGGA CCTTATCAGC TACGTGGTGA ACATGGGTCT GATTGACAAA CTGTGTGCCT 
     4381 GCTTCCTCTC GGTGCAAGGC CCAGTGGATG AGAATCCCAA GATGGCCATA TTTCTGCAGC 
     4441 ATGCCGCAGG ACTCTTACAT GCAATGTGTA CACTGTGCTT TGCTGTCACT GGAAGGTCAT 
     4501 ACAGCATATT TGACAATAAT CGCCAGGATC CCACAGGGCT GACAGCTGCT CTTCAGGCAA 
     4561 CCGACCTGGC TGGAGTTCTT CATATGCTCT ACTGTGTCCT CTTCCATGGC ACCATCTTGG 
     4621 ACCCCAGCAC TGCCAGTCCC AAGGAGAATT ACACTCAAAA TACCATCCAA GTGGCCATTC 
     4681 AGAGTTTACG TTTCTTCAAC AGCTTTGCAG CTCTTCATCT GCCTGCTTTT CAGTCTATTG 
     4741 TAGGGGCAGA GGGCTTGTCC CTTGCATTCC GGCACATGGC CAGCTCCCTG CTGGGCCACT 
     4801 GCAGCCAAGT CTCCTGTGAA AGCCTCCTTC ATGAGGTCAT CGTCTGTGTG GGCTACTTCA 
     4861 CTGTCAACCA CCCAGATAAC CAGGTGATCG TGCAGTCCGG CCGCCACCCC ACAGTGCTGC 
     4921 AGAAGCTCTG CCAGTTGCCC TTCCAGTATT TCAGTGACCC ACGGCTGATC AAAGTACTGT 
     4981 TCCCTTCACT TATCGCTGCT TGTTACAACA ACCATCAGAA CAAGATCATT CTGGAGCAAG 
     5041 AGATGAGCTG TGTTTTACTG GCCACTTTCA TTCAGGATTT GGCACAGACT CCAGGTCAAG 
     5101 CGGAAAACCA GCCTTACCAA CCCAAAGGGA AATGCCTTGG TTCCCAAGAC TATCTTGAGC 
     5161 TGGCTAACAG ATTTCCTCAG CAGGCCTGGG AAGAAGCTCG ACAGTTTTTC TTGAAAAAAG 
     5221 AGAAAAAAAC GCGTACGCGG CCGCTCGAGC AGAAACTCAT CTCAGAAGAG GATCTGGCAG 
     5281 CAAATGATAT CCTGGATTAC AAGGATGACG ACGATAAGGT TTAAACGGCC GGCCGCGGTC 
     5341 ATAGCTGTTT CCTGAACAGA TCCCGGGTGG CATCCCTGTG ACCCCTCCCC AGTGCCTCTC 
     5401 CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC ACCAGCCTTG TCCTAATAAA ATTAAGTTGC 
     5461 ATCATTTTGT CTGACTAGGT GTCCTTCTAT AATATTATGG GGTGGAGGGG GGTGGTATGG 
     5521 AGCAAGGGGC AAGTTGGGAA GACAACCTGT AGGGCCTGCG GGGTCTATTG GGAACCAAGC 
     5581 TGGAGTGCAG TGGCACAATC TTGGCTCACT GCAATCTCCG CCTCCTGGGT TCAAGCGATT 
     5641 CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA TTCCAGGCAT GCATGACCAG GCTCAGCTAA 
     5701 TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC ACCATATTGG CCAGGCTGGT CTCCAACTCC 
     5761 TAATCTCAGG TGATCTACCC ACCTTGGCCT CCCAAATTGC TGGGATTACA GGCGTGAACC 
     5821 ACTGCTCCCT TCCCTGTCCT TCTGATTTTA AAATAACTAT ACCAGCAGGA GGACGTCCAG 
     5881 ACACAGCATA GGCTACCTGG CCATGCCCAA CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC 
     5941 ACTGTCCTCT CATGCGTTGG GTCCACTCAG TAGATGCCTG TTGAATTGGG TACGCGGCCA 
     6001 GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA 
     6061 TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC 
     6121 CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG 
     6181 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG 
     6241 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT 
     6301 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT 
     6361 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG 
     6421 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT 
     6481 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT 
     6541 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT 
     6601 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC 
     6661 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC 
     6721 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG 
     6781 TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA 
     6841 AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA CCTGAGGCTA TGGCAGGGCC 
     6901 TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG GCCTTCACCC GAACTTGGGG GGTGGGGTGG 
     6961 GGAAAAGGAA GAAACGCGGG CGTATTGGCC CCAATGGGGT CTCGGTGGGG TATCGACAGA 
     7021 GTGCCAGCCC TGGGACCGAA CCCCGCGTTT ATGAACAAAC GACCCAACAC CGTGCGTTTT 
     7081 ATTCTGTCTT TTTATTGCCG TCATAGCGCG GGTTCCTTCC GGTATTGTCT CCTTCCGTGT 
     7141 TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA GAACTCCAGC ATGAGATCCC CGCGCTGGAG 
     7201 GATCATCCAG CCGGCGTCCC GGAAAACGAT TCCGAAGCCC AACCTTTCAT AGAAGGCGGC 
     7261 GGTGGAATCG AAATCTCGTG ATGGCAGGTT GGGCGTCGCT TGGTCGGTCA TTTCGAACCC 
     7321 CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA AGGCGATAGA AGGCGATGCG CTGCGAATCG 
     7381 GGAGCGGCGA TACCGTAAAG CACGAGGAAG CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA 
     7441 GCAATATCAC GGGTAGCCAA CGCTATGTCC TGATAGCGAT CCGCCACACC CAGCCGGCCA 
     7501 CAGTCGATGA ATCCAGAAAA GCGGCCATTT TCCACCATGA TATTCGGCAA GCAGGCATCG 
     7561 CCATGGGTCA CGACGAGATC CTCGCCGTCG GGCATGCTCG CCTTGAGCCT GGCGAACAGT 
     7621 TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG TCCAGATCAT CCTGATCGAC AAGACCGGCT 
     7681 TCCATCCGAG TACGTGCTCG CTCGATGCGA TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA 
     7741 GCCGGATCAA GCGTATGCAG CCGCCGCATT GCATCAGCCA TGATGGATAC TTTCTCGGCA 
     7801 GGAGCAAGGT GAGATGACAG GAGATCCTGC CCCGGCACTT CGCCCAATAG CAGCCAGTCC 
     7861 CTTCCCGCTT CAGTGACAAC GTCGAGCACA GCTGCGCAAG GAACGCCCGT CGTGGCCAGC 
     7921 CACGATAGCC GCGCTGCCTC GTCTTGCAGT TCATTCAGGG CACCGGACAG GTCGGTCTTG 
     7981 ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC AGCCGGAACA CGGCGGCATC AGAGCAGCCG 
     8041 ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT AGCCTCTCCA CCCAAGCGGC CGGAGAACCT 
     8101 GCGTGCAATC CATCTTGTTC AATCATGCGA AACGATCCTC ATCCTGTCTC TTGATCGATC 
     8161 TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC TCCTCACTAC TTCTGGAATA GCTCAGAGGC 
     8221 CGAGGCGGCC TCGGCCTCTG CATAAATAAA AAAAATTAGT CAGCCATGGG GCGGAGAATG 
     8281 GGCGGAACTG GGCGGAGTTA GGGGCGGGAT GGGCGGAGTT AGGGGCGGGA CTATGGTTGC 
     8341 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     8401 ACACCTGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     8461 TGGGGACTTT CCACACCCTA ACTGACACAC ATTCCACAGC TGGTTCTTTC CGCCTCAGGA 
     8521 CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC 
     8581 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA 
     8641 GTGCCACCTG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC 
     8701 AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC 
     8761 TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG 
     8821 TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA 
     8881 CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC 
     8941 TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT 
     9001 TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA 
     9061 CAAAAATTTA ACGCGAATTT T
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