LOCUS       RC229431                8895 bp    DNA     circular  SYN 
DEFINITION  GPR124 (Myc-DDK-tagged)-Human G protein-coupled receptor 124 (GPR124)
ACCESSION   NM_032777
VERSION     NM_032777.7
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5052..5143 
                     /label="Myc-DDK"  
     misc_feature    5173..5758 
                     /label="PolyA signal"  
     misc_feature    5230..5249 
                     /label="Restriction Enzyme"  
     misc_feature    6034..6706 
                     /label="ColE1"  
     misc_feature    7136..7930 
                     /label="Kan/Neo"  
     misc_feature    7965..8267 
                     /label="SV40 ori"  
     misc_feature    8353..8830 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5034..5040 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5034 
                     /label="ADGRA2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGCGCCGGG GGACGCAGGA TGCGGGGGGC GCCCGCGCGC CTGCTGCTGC 
     1081 CGCTGCTGCC GTGGCTCCTG CTGCTCCTGG CGCCCGAGGC TCGGGGCGCG CCCGGCTGCC 
     1141 CGCTATCCAT CCGCAGCTGC AAGTGCTCGG GGGAGCGGCC CAAGGGGCTG AGCGGCGGCG 
     1201 TCCCTGGCCC GGCTCGGCGG AGGGTGGTGT GCAGCGGCGG GGACCTCCCG GAGCCTCCCG 
     1261 AGCCCGGCCT TCTGCCTAAC GGCACCGTTA CCCTGCTCTT GAGCAATAAC AAGATCACGG 
     1321 GGCTCCGCAA TGGCTCCTTC CTGGGACTGT CACTGCTGGA GAAGCTGGAC CTGAGGAACA 
     1381 ACATCATCAG CACAGTGCAG CCGGGCGCCT TCCTGGGCCT GGGGGAGCTG AAGCGTTTAG 
     1441 ATCTCTCCAA CAACCGGATT GGCTGTCTCA CCTCCGAGAC CTTCCAGGGC CTCCCCAGGC 
     1501 TTCTCCGACT AAACATATCT GGAAACATCT TCTCCAGTCT GCAACCTGGG GTCTTTGATG 
     1561 AGCTGCCAGC CCTTAAGGTT GTGGACTTGG GCACCGAGTT CCTGACCTGT GACTGCCACC 
     1621 TGCGCTGGCT GCTGCCCTGG GCCCAGAATC GCTCCCTGCA GCTGTCGGAA CACACGCTCT 
     1681 GTGCTTACCC CAGTGCCCTG CATGCTCAGG CCCTGGGCAG CCTCCAGGAG GCCCAGCTCT 
     1741 GCTGCGAGGG GGCCCTGGAG CTGCACACAC ACCACCTCAT CCCGTCCCTA CGCCAAGTGG 
     1801 TGTTCCAGGG GGATCGGCTG CCCTTCCAGT GCTCTGCCAG CTACCTGGGC AACGACACCC 
     1861 GCATCCGCTG GTACCACAAC CGAGCCCCTG TGGAGGGTGA TGAGCAGGCG GGCATCCTCC 
     1921 TGGCCGAGAG CCTCATCCAC GACTGCACCT TCATCACCAG TGAGCTGACG CTGTCTCACA 
     1981 TCGGCGTGTG GGCCTCAGGC GAGTGGGAGT GCACCGTGTC CATGGCCCAA GGCAACGCCA 
     2041 GCAAGAAGGT GGAGATCGTG GTGCTGGAGA CCTCTGCCTC CTACTGCCCC GCCGAGCGTG 
     2101 TTGCCAACAA CCGCGGGGAC TTCAGGTGGC CCCGAACTCT GGCTGGCATC ACAGCCTACC 
     2161 AGTCCTGCCT GCAGTATCCC TTCACCTCAG TGCCCCTGGG CGGGGGTGCC CCGGGCACCC 
     2221 GAGCCTCCCG CCGGTGTGAC CGTGCCGGCC GCTGGGAGCC AGGGGACTAC TCCCACTGTC 
     2281 TCTACACCAA CGACATCACC AGGGTGCTGT ACACCTTCGT GCTGATGCCC ATCAATGCCT 
     2341 CCAATGCGCT GACCCTGGCT CACCAGCTGC GCGTGTACAC AGCCGAGGCC GCTAGCTTTT 
     2401 CAGACATGAT GGATGTAGTC TATGTGGCTC AGATGATCCA GAAATTTTTG GGTTATGTCG 
     2461 ACCAGATCAA AGAGCTGGTA GAGGTGATGG TGGACATGGC CAGCAACCTG ATGCTGGTGG 
     2521 ACGAGCACCT GCTGTGGCTG GCCCAGCGCG AGGACAAGGC CTGCAGCCGC ATCGTGGGTG 
     2581 CCCTGGAGCG CATTGGGGGG GCCGCCCTCA GCCCCCATGC CCAGCACATC TCAGTGAATG 
     2641 CGAGGAACGT GGCATTGGAG GCCTACCTCA TCAAGCCGCA CAGCTACGTG GGCCTGACCT 
     2701 GCACAGCCTT CCAGAGGAGG GAGGGAGGGG TGCCGGGCAC ACGGCCAGGA AGCCCTGGCC 
     2761 AGAACCCCCC ACCTGAGCCC GAGCCCCCAG CTGACCAGCA GCTCCGCTTC CGCTGCACCA 
     2821 CCGGGAGGCC CAATGTTTCT CTGTCGTCCT TCCACATCAA GAACAGCGTG GCCCTGGCCT 
     2881 CCATCCAGCT GCCCCCGAGT CTATTCTCAT CCCTTCCGGC TGCCCTGGCT CCCCCGGTGC 
     2941 CCCCAGACTG CACCCTGCAA CTGCTCGTCT TCCGAAATGG CCGCCTCTTC CACAGCCACA 
     3001 GCAACACCTC CCGCCCTGGA GCTGCTGGGC CTGGCAAGAG GCGTGGCGTG GCCACCCCCG 
     3061 TCATCTTCGC AGGAACCAGT GGCTGTGGCG TGGGAAACCT GACAGAGCCA GTGGCCGTTT 
     3121 CGCTGCGGCA CTGGGCTGAG GGAGCCGAAC CTGTGGCCGC TTGGTGGAGC CAGGAGGGGC 
     3181 CCGGGGAGGC TGGGGGCTGG ACCTCGGAGG GCTGCCAGCT CCGCTCCAGC CAGCCCAATG 
     3241 TCAGCGCCCT GCACTGCCAG CACTTGGGCA ATGTGGCCGT GCTCATGGAG CTGAGCGCCT 
     3301 TTCCCAGGGA GGTGGGGGGC GCCGGGGCAG GGCTGCACCC CGTGGTATAC CCCTGCACGG 
     3361 CCTTGCTGCT GCTCTGCCTC TTCGCCACCA TCATCACCTA CATCCTCAAC CACAGCTCCA 
     3421 TCCGTGTGTC CCGGAAAGGC TGGCACATGC TGCTGAACTT GTGCTTCCAC ATAGCCATGA 
     3481 CCTCTGCTGT CTTTGCGGGG GGCATCACAC TCACCAACTA CCAGATGGTC TGCCAGGCGG 
     3541 TGGGCATCAC CCTGCACTAC TCCTCCCTAT CCACGCTGCT CTGGATGGGC GTGAAGGCGC 
     3601 GAGTGCTCCA TAAGGAGCTC ACCTGGAGGG CACCCCCTCC GCAAGAAGGG GACCCCGCTC 
     3661 TGCCTACTCC CAGTCCTATG CTCCGGTTCT ATTTGATCGC TGGAGGGATT CCACTCATTA 
     3721 TCTGTGGCAT CACAGCTGCA GTCAACATCC ACAACTACCG GGACCACAGC CCCTACTGCT 
     3781 GGCTGGTGTG GCGTCCAAGC CTTGGCGCCT TCTACATCCC TGTGGCTTTG ATTCTGCTCA 
     3841 TCACCTGGAT CTATTTCCTG TGCGCCGGGC TACGCTTACG GGGTCCTCTG GCACAGAACC 
     3901 CCAAGGCGGG CAACAGCAGG GCCTCCCTGG AGGCAGGGGA GGAGCTGAGG GGTTCCACCA 
     3961 GGCTCAGGGG CAGCGGCCCC CTCCTGAGTG ACTCAGGTTC CCTTCTTGCT ACTGGGAGCG 
     4021 CGCGAGTGGG GACGCCCGGG CCCCCGGAGG ATGGTGACAG CCTCTATTCT CCGGGAGTCC 
     4081 AGCTAGGGGC GCTGGTGACC ACGCACTTCC TGTACTTGGC CATGTGGGCC TGCGGGGCTC 
     4141 TGGCAGTGTC CCAGCGCTGG CTGCCCCGGG TGGTGTGCAG CTGCTTGTAC GGGGTGGCAG 
     4201 CCTCCGCCCT GGGCCTCTTC GTCTTCACTC ACCACTGTGC CAGGCGGAGG GACGTGAGAG 
     4261 CCTCGTGGCG CGCCTGCTGC CCCCCTGCCT CTCCCGCGGC CCCCCATGCC CCGCCCCGGG 
     4321 CCCTGCCCGC CGCCGCAGAG GACGGTTCCC CGGTGTTCGG GGAGGGCCCC CCCTCCCTCA 
     4381 AGTCCTCCCC AAGCGGCAGC AGCGGCCATC CGCTGGCTCT GGGCCCCTGC AAGCTCACCA 
     4441 ACCTGCAGCT GGCCCAGAGT CAGGTGTGCG AGGCGGGGGC GGCGGCCGGC GGGGAAGGAG 
     4501 AGCCGGAGCC GGCGGGCACC CGGGGAAACC TCGCCCACCG CCACCCCAAC AACGTGCACC 
     4561 ACGGGCGTCG GGCGCACAAG AGCCGGGCCA AGGGACACCG CGCGGGGGAG GCCTGCGGCA 
     4621 AGAACCGGCT CAAGGCCCTG CGCGGGGGCG CGGCGGGGGC GCTGGAGCTG CTGTCCAGCG 
     4681 AGAGCGGCAG TCTGCACAAC AGCCCCACCG ACAGCTACCT GGGCAGCAGC CGCAACAGCC 
     4741 CGGGCGCCGG CCTGCAGCTG GAAGGCGAGC CCATGCTCAC GCCGTCCGAG GGCAGCGACA 
     4801 CCAGCGCCGC GCCGCTTTCT GAGGCGGGCC GGGCAGGCCA GCGCCGCAGC GCCAGCCGCG 
     4861 ACAGTCTCAA GGGCGGCGGC GCGCTGGAGA AGGAGAGCCA TCGCCGCTCG TACCCGCTCA 
     4921 ACGCCGCCAG CCTAAACGGC GCCCCCAAGG GGGGCAAGTA CGACGACGTC ACCCTGATGG 
     4981 GCGCGGAGGT AGCCAGCGGC GGCTGCATGA AGACCGGACT CTGGAAGAGC GAAACTACCG 
     5041 TCACGCGTAC GCGGCCGCTC GAGCAGAAAC TCATCTCAGA AGAGGATCTG GCAGCAAATG 
     5101 ATATCCTGGA TTACAAGGAT GACGACGATA AGGTTTAAAC GGCCGGCCGC GGTCATAGCT 
     5161 GTTTCCTGAA CAGATCCCGG GTGGCATCCC TGTGACCCCT CCCCAGTGCC TCTCCTGGCC 
     5221 CTGGAAGTTG CCACTCCAGT GCCCACCAGC CTTGTCCTAA TAAAATTAAG TTGCATCATT 
     5281 TTGTCTGACT AGGTGTCCTT CTATAATATT ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG 
     5341 GGGCAAGTTG GGAAGACAAC CTGTAGGGCC TGCGGGGTCT ATTGGGAACC AAGCTGGAGT 
     5401 GCAGTGGCAC AATCTTGGCT CACTGCAATC TCCGCCTCCT GGGTTCAAGC GATTCTCCTG 
     5461 CCTCAGCCTC CCGAGTTGTT GGGATTCCAG GCATGCATGA CCAGGCTCAG CTAATTTTTG 
     5521 TTTTTTTGGT AGAGACGGGG TTTCACCATA TTGGCCAGGC TGGTCTCCAA CTCCTAATCT 
     5581 CAGGTGATCT ACCCACCTTG GCCTCCCAAA TTGCTGGGAT TACAGGCGTG AACCACTGCT 
     5641 CCCTTCCCTG TCCTTCTGAT TTTAAAATAA CTATACCAGC AGGAGGACGT CCAGACACAG 
     5701 CATAGGCTAC CTGGCCATGC CCAACCGGTG GGACATTTGA GTTGCTTGCT TGGCACTGTC 
     5761 CTCTCATGCG TTGGGTCCAC TCAGTAGATG CCTGTTGAAT TGGGTACGCG GCCAGCGGCG 
     5821 AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC 
     5881 AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT 
     5941 GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG 
     6001 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC 
     6061 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC 
     6121 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT 
     6181 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT 
     6241 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC 
     6301 AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA 
     6361 GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA 
     6421 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG 
     6481 TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA 
     6541 AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG 
     6601 GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG 
     6661 AAGTTTTAAA TCAATCTAAA GTATATATGA GTAACCTGAG GCTATGGCAG GGCCTGCCGC 
     6721 CCCGACGTTG GCTGCGAGCC CTGGGCCTTC ACCCGAACTT GGGGGGTGGG GTGGGGAAAA 
     6781 GGAAGAAACG CGGGCGTATT GGCCCCAATG GGGTCTCGGT GGGGTATCGA CAGAGTGCCA 
     6841 GCCCTGGGAC CGAACCCCGC GTTTATGAAC AAACGACCCA ACACCGTGCG TTTTATTCTG 
     6901 TCTTTTTATT GCCGTCATAG CGCGGGTTCC TTCCGGTATT GTCTCCTTCC GTGTTTCAGT 
     6961 TAGCCTCCCC CTAGGGTGGG CGAAGAACTC CAGCATGAGA TCCCCGCGCT GGAGGATCAT 
     7021 CCAGCCGGCG TCCCGGAAAA CGATTCCGAA GCCCAACCTT TCATAGAAGG CGGCGGTGGA 
     7081 ATCGAAATCT CGTGATGGCA GGTTGGGCGT CGCTTGGTCG GTCATTTCGA ACCCCAGAGT 
     7141 CCCGCTCAGA AGAACTCGTC AAGAAGGCGA TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG 
     7201 GCGATACCGT AAAGCACGAG GAAGCGGTCA GCCCATTCGC CGCCAAGCTC TTCAGCAATA 
     7261 TCACGGGTAG CCAACGCTAT GTCCTGATAG CGATCCGCCA CACCCAGCCG GCCACAGTCG 
     7321 ATGAATCCAG AAAAGCGGCC ATTTTCCACC ATGATATTCG GCAAGCAGGC ATCGCCATGG 
     7381 GTCACGACGA GATCCTCGCC GTCGGGCATG CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT 
     7441 GGCGCGAGCC CCTGATGCTC TTCGTCCAGA TCATCCTGAT CGACAAGACC GGCTTCCATC 
     7501 CGAGTACGTG CTCGCTCGAT GCGATGTTTC GCTTGGTGGT CGAATGGGCA GGTAGCCGGA 
     7561 TCAAGCGTAT GCAGCCGCCG CATTGCATCA GCCATGATGG ATACTTTCTC GGCAGGAGCA 
     7621 AGGTGAGATG ACAGGAGATC CTGCCCCGGC ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC 
     7681 GCTTCAGTGA CAACGTCGAG CACAGCTGCG CAAGGAACGC CCGTCGTGGC CAGCCACGAT 
     7741 AGCCGCGCTG CCTCGTCTTG CAGTTCATTC AGGGCACCGG ACAGGTCGGT CTTGACAAAA 
     7801 AGAACCGGGC GCCCCTGCGC TGACAGCCGG AACACGGCGG CATCAGAGCA GCCGATTGTC 
     7861 TGTTGTGCCC AGTCATAGCC GAATAGCCTC TCCACCCAAG CGGCCGGAGA ACCTGCGTGC 
     7921 AATCCATCTT GTTCAATCAT GCGAAACGAT CCTCATCCTG TCTCTTGATC GATCTTTGCA 
     7981 AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA CTACTTCTGG AATAGCTCAG AGGCCGAGGC 
     8041 GGCCTCGGCC TCTGCATAAA TAAAAAAAAT TAGTCAGCCA TGGGGCGGAG AATGGGCGGA 
     8101 ACTGGGCGGA GTTAGGGGCG GGATGGGCGG AGTTAGGGGC GGGACTATGG TTGCTGACTA 
     8161 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCT 
     8221 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     8281 CTTTCCACAC CCTAACTGAC ACACATTCCA CAGCTGGTTC TTTCCGCCTC AGGACTCTTC 
     8341 CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT 
     8401 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA 
     8461 CCTGACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG 
     8521 ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC 
     8581 GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GGCTCCCTTT AGGGTTCCGA 
     8641 TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT AGGGTGATGG TTCACGTAGT 
     8701 GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT 
     8761 AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGTCTA TTCTTTTGAT 
     8821 TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA 
     8881 TTTAACGCGA ATTTT
//