LOCUS       RC229428                8850 bp    DNA     circular  SYN 
DEFINITION  MROH7 (Myc-DDK-tagged)-Human HEAT repeat containing 8 (HEATR8), transcript variant 1
ACCESSION   NM_001039464
VERSION     NM_001039464.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5007..5098 
                     /label="Myc-DDK"  
     misc_feature    5128..5713 
                     /label="PolyA signal"  
     misc_feature    5185..5204 
                     /label="Restriction Enzyme"  
     misc_feature    5989..6661 
                     /label="ColE1"  
     misc_feature    7091..7885 
                     /label="Kan/Neo"  
     misc_feature    7920..8222 
                     /label="SV40 ori"  
     misc_feature    8308..8785 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4989..4995 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4989 
                     /label="MROH7"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCCCTGAGT CCAGGGGCTA ACCTGGTCTT CCATGAAGAC CCAAAGATGA 
     1081 CACCAAGTCC CCCCTCCTGT GGGGCCCCGG GATTAGGGTC TGGTACCATC CCTCAGCCCC 
     1141 ACCCAGACAT GGCTCAGGTG CCTATGTTGA ATCTGCTCCC AAGTCCTGGC TTGGCTCTCG 
     1201 TTCCAGATCT TAATGATTCT TTGAGTCCAG TCTCAGGGGA GGCCTCAGGC CTGGTGTCTG 
     1261 AAAACACCCC CAGACCTGAT GACAGCAGAG CTATCGCTCC AGCCTCCCTC CAGATCACCA 
     1321 GTTCTTGTTC TGGTGAAGCC CTGGACCTGG ATTCCAAGGA TGTCTCAAGG CCTGACTCAC 
     1381 AGGGGCGCCT CTGTCCAGCC TCAAACCCCA TTCTGAGCCC TAGCTCTACT GAGGCCCCTC 
     1441 GTCTGAGCTC TGGGAACCAC CCTCAGTCAA ATTCTGAAGA TGCCTTCAAG TGCCTCTCAA 
     1501 GTAAGATTTT CAAGTTGGGT CAGAGAAACT CCAACCCTTC TAGGCATGAA TTAAACCCAT 
     1561 TTATAAGGCA CCATTCCAGA GAAGGTCTGG TTCTGGGCCA CTGCATCTCT AGGCCAAGCT 
     1621 CAAAAGCACT CCTTATTCCA ACCTCAAACT CTTCTCTGGA CCTTGACTCC AATCCATTGC 
     1681 TCAACATGGG CTCAAGAAAC ACCTCCAAGC TGAACCTGAA TGTAGCTCCA GATTCTCATG 
     1741 GGACCCTAAT CCCAGACACA AATGAGACCA TCACTTTGGC TTCACATAAT ATCTCTGAGT 
     1801 CTGTTTCAAA AGGAGCCTTT AGTACCACCT GGAGCACAAG TTCAAAGGAA ACCATGAATG 
     1861 TGGCTTCCAG CGGCCACTCC AGATCTGATT TGAGCGTGAC CATCACTCAA GCCTCGTATG 
     1921 TGACCCTGAT TCCTGGCTCC AGCTATGGTA TCAGCCTGCA CTCCAGCACC CATGGGCCCA 
     1981 ACTCCACCAT CTCTCCACCC TCATGCATGA CTCTAATCCT GGGTTCCAAT GAGACTCTGA 
     2041 GCCTGGACTC CAGCCTCCTG TTCAGCGACA CCTCCACCTT GACGCTGAGC AGTCAGCAGG 
     2101 ATGATGCCAA GGACAACAGC ATCCACACTG TGCCCCTGGA GGAGAATCTG GAGAGTTGGA 
     2161 GTGAGATGGC CAGCATTAAG GTGGGCCAGT TCCCGCTGGG ATTCCCCATC TCCAACCCCG 
     2221 CAGGCAAGGA CGCCGTGACC TTGCAAGGCA TCCCTGAGGG AGCCTTTGAT GAAGTGACCT 
     2281 CATGCCTGGT GAAGGTGCCA GAGAAGACAG AAGGTGGCAA CAACATGGCT CTGGTTGAGA 
     2341 ATGTCACCAC CCTTCAGAAG AGCCAGGATC TGCTGGAGGC AGAAGGAGAA AAGAAGACCA 
     2401 TGATAAAGAA GATTATGAGG CAGATCCAGG AGGAGCCACT GGATTCTCTC TCAAGCTCCG 
     2461 TCCGCAAGCA GGCCATGGAG ATCCTGACCC AGCTGAGCCA CACCCAGCCC ACCCTGGGCA 
     2521 TGCGGGAGAG GTCGGAGCTG GTGAACGTGT GTGTGCACAG CGTGTTCTCC CTGCCCTCCG 
     2581 TGCAGGCGAT GCAGGAGAAG GACGAGGCCA AGGCTGAGAC CATCCAGGCT CTTTACCATC 
     2641 AGACCCTGGA GGCCCTGCAG ACACTGCTCA AAGCCCTCTT TATCGAGGAC CCCACTCCTG 
     2701 CTGGGCTGAA GAGCATCTTG GAGGCCCTGG GGCCTTGGAT GAACTCTGGG AAGGCCCATG 
     2761 AGCGAGCACG GGCTGTGAAC ACCAATGTCT CTGTGTTGAA CCACATGCTT CTAACTCTGC 
     2821 CTTTCTTTAT GCCCTTGGGG TTCCCGGCGC TGGGGCTTCT GCTGGGGAGA CTCATCCTTC 
     2881 ACATTGGGGA TCCTGATGAG GAGATTGGCT GTGAGGCTCT GGACGGCATC ATCATCCTCT 
     2941 ACACTATTCT GGAGCTCCAA AAACGAGCCA GAGATAAGGA AGAGACCAAC AAAAAGGAGC 
     3001 TATATGAGAG CAACAAGCAT TTCCTGGGGC CCTACAACCC TGTGAGCCCG TGCCAGAACA 
     3061 TTCTGCGGGT GATCGAGGAA TTTGGAGACT TCCTGGGGCC CCAGCAGATA AAGGACCTGC 
     3121 TGCTGGCCGC CCTGGAAGGG CTGAAAGGCA GCTCAGAGGC TCCAGGGAAG GACTCCAGGG 
     3181 AGATGATGCA GCTGGCCTCG GAGGTCATGC TCAGCTCGGT GCTGGAGTGG TACCGCCACA 
     3241 GGGCGCTGGA GGTGATCCCA GAAATCATGC AAGGCATCTA CATGCAGCTG AGCCACATCC 
     3301 AGGAGCCTCG GGCCCGCCAG GTGGCCCTGC TGCCCGTCTC CCTCCTGGCT AGCTCCTTCA 
     3361 TGACCGAGGT TGTGGTGGCC CTGCTCATGT GCCCCCTCCC ACTGAACAGC AATGGAGCAG 
     3421 AGATGTGGAG GCAGCTGATA CTGTGTAAGC CCAGCTGTGA TGTCCGAGAC CTCCTGGATC 
     3481 TGCTCCTGGG CAGCCTGAAG GAGAAGCCCG TCACCAAGGA GGGCCGGGCT TCCATCGTGC 
     3541 CCCTGGCGGC AGCCAGCGGC CTGTGCGAGC TCCTGTCCGT CAACAGCTGC ATGGGCCGTG 
     3601 TGAGGCGCAT CTACCCTCAG CTGCTCCTGG CCCTGCTCAT TCAGGTCCAT TACCACATCG 
     3661 GCCTCAACCT GCCTGGCTGC GTGGCTCCTC CCAAGGACAC CAAGAAGGGT GCACAGCCCT 
     3721 CTCCCTTCGT ACCTGTGCGC TGGGTGGTGA AAGTGGTGAA AACCCTGCTA CTGAGGATGG 
     3781 GCTGCTCTTA TGAGACCACG TTTCTGGAGG ACCAGGGTGG CTGGGAGCTC ATGGAGCAGG 
     3841 TGGAGAGCCA CCACCGCGGA GTGGCCTTGC TGGCAAGGGC CATGGTGCAG TACTCCTGCC 
     3901 AGGAGCTGTG CCGCATCCTC TACCTGCTCA TCCCGCTCCT GGAGCGAGGC GACGAGAAGC 
     3961 ACAGGATCAC GGCCACCGCC TTCTTCGTGG AGCTCCTCCA GATGGAGCAG GTGCGCCGGA 
     4021 TCCCCGAGGA ATACTCTCTG GGGCGGATGG CAGAAGGCCT GAGCCACCAC GACCCCATCA 
     4081 TGAAGGTGCT GTCCATTCGA GGCCTGGTCA TCCTGGCCCG CAGGTCTGAG AAGACCGCCA 
     4141 AGGTGAAGGC CCTCCTGCCC TCCATGGTGA AGGGCCTGAA GAACATGGAT GGGATGCTGG 
     4201 TGGTGGAAGC GGTCCACAAC CTCAAGGCTG TCTTCAAGGG GCGGGACCAG AAGCTGATGG 
     4261 ACAGTGCGGT CTATGTGGAG ATGCTGCAGA TCCTGCTGCC GCACTTCAGC GACGCACGAG 
     4321 AGGTCGTGCG CTCCTCCTGC ATCAACCTGT ATGGGAAGGT GGTCCAGAAG CTTCGGGCAC 
     4381 CACGCACTCA GGCCATGGAG GAGCAGCTGG TCAGCACCTT GGTGCCCCTA CTGCTGACCA 
     4441 TGCAGGAGGG CAACTCCAAG GTAAGCCAGA AGTGTGTGAA GACCCTGTTA CGCTGTTCTT 
     4501 ACTTCATGGC TTGGGAGTTG CCAAAAAGAG CTTATAGCCG GAAGCCCTGG GACAACCAAC 
     4561 AGCAGACAGT GGCCAAAATT TGCAAGTGCC TTGTGAACAC CCACCGAGAC AGCGCCTTCA 
     4621 TATTCCTCAG CCAGAGCCTG GAGTATGCCA AGAACTCACG GGCCTCCCTC CGGAAGTGCT 
     4681 CAGTCATGTT CATAGGGTCC CTGGTCCCCT GCATGGAGAG CATAATGACA GAAGATCGTC 
     4741 TGAATGAAGT GAAAGCTGCT CTGGATAACT TGAGACATGA CCCAGAAGCA TCAGTGTGCA 
     4801 TCTACGCAGC CCAGGTCCAG GACCACATCC TGGCCAGCTG CTGGCAGAAC TCCTGGCTGC 
     4861 CGCACGGGAA CTCATGGGTG TGTTACTCAG CCACCACCCA CCGCTGGAGC CCCAGCTGTG 
     4921 AGAACCTGCC CACTTCCCAC CAGCGGCGCT CCTGGATCAT GCAGGCACTG GGCTCCTGGA 
     4981 AGATGTCCTT GAAGAAGACG CGTACGCGGC CGCTCGAGCA GAAACTCATC TCAGAAGAGG 
     5041 ATCTGGCAGC AAATGATATC CTGGATTACA AGGATGACGA CGATAAGGTT TAAACGGCCG 
     5101 GCCGCGGTCA TAGCTGTTTC CTGAACAGAT CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA 
     5161 GTGCCTCTCC TGGCCCTGGA AGTTGCCACT CCAGTGCCCA CCAGCCTTGT CCTAATAAAA 
     5221 TTAAGTTGCA TCATTTTGTC TGACTAGGTG TCCTTCTATA ATATTATGGG GTGGAGGGGG 
     5281 GTGGTATGGA GCAAGGGGCA AGTTGGGAAG ACAACCTGTA GGGCCTGCGG GGTCTATTGG 
     5341 GAACCAAGCT GGAGTGCAGT GGCACAATCT TGGCTCACTG CAATCTCCGC CTCCTGGGTT 
     5401 CAAGCGATTC TCCTGCCTCA GCCTCCCGAG TTGTTGGGAT TCCAGGCATG CATGACCAGG 
     5461 CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA CGGGGTTTCA CCATATTGGC CAGGCTGGTC 
     5521 TCCAACTCCT AATCTCAGGT GATCTACCCA CCTTGGCCTC CCAAATTGCT GGGATTACAG 
     5581 GCGTGAACCA CTGCTCCCTT CCCTGTCCTT CTGATTTTAA AATAACTATA CCAGCAGGAG 
     5641 GACGTCCAGA CACAGCATAG GCTACCTGGC CATGCCCAAC CGGTGGGACA TTTGAGTTGC 
     5701 TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG TCCACTCAGT AGATGCCTGT TGAATTGGGT 
     5761 ACGCGGCCAG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA 
     5821 ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG 
     5881 TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA 
     5941 AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT 
     6001 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT 
     6061 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT 
     6121 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC 
     6181 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT 
     6241 ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC 
     6301 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT 
     6361 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA 
     6421 ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA 
     6481 AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA 
     6541 AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT 
     6601 TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAC CTGAGGCTAT 
     6661 GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC GAGCCCTGGG CCTTCACCCG AACTTGGGGG 
     6721 GTGGGGTGGG GAAAAGGAAG AAACGCGGGC GTATTGGCCC CAATGGGGTC TCGGTGGGGT 
     6781 ATCGACAGAG TGCCAGCCCT GGGACCGAAC CCCGCGTTTA TGAACAAACG ACCCAACACC 
     6841 GTGCGTTTTA TTCTGTCTTT TTATTGCCGT CATAGCGCGG GTTCCTTCCG GTATTGTCTC 
     6901 CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG GTGGGCGAAG AACTCCAGCA TGAGATCCCC 
     6961 GCGCTGGAGG ATCATCCAGC CGGCGTCCCG GAAAACGATT CCGAAGCCCA ACCTTTCATA 
     7021 GAAGGCGGCG GTGGAATCGA AATCTCGTGA TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT 
     7081 TTCGAACCCC AGAGTCCCGC TCAGAAGAAC TCGTCAAGAA GGCGATAGAA GGCGATGCGC 
     7141 TGCGAATCGG GAGCGGCGAT ACCGTAAAGC ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA 
     7201 AGCTCTTCAG CAATATCACG GGTAGCCAAC GCTATGTCCT GATAGCGATC CGCCACACCC 
     7261 AGCCGGCCAC AGTCGATGAA TCCAGAAAAG CGGCCATTTT CCACCATGAT ATTCGGCAAG 
     7321 CAGGCATCGC CATGGGTCAC GACGAGATCC TCGCCGTCGG GCATGCTCGC CTTGAGCCTG 
     7381 GCGAACAGTT CGGCTGGCGC GAGCCCCTGA TGCTCTTCGT CCAGATCATC CTGATCGACA 
     7441 AGACCGGCTT CCATCCGAGT ACGTGCTCGC TCGATGCGAT GTTTCGCTTG GTGGTCGAAT 
     7501 GGGCAGGTAG CCGGATCAAG CGTATGCAGC CGCCGCATTG CATCAGCCAT GATGGATACT 
     7561 TTCTCGGCAG GAGCAAGGTG AGATGACAGG AGATCCTGCC CCGGCACTTC GCCCAATAGC 
     7621 AGCCAGTCCC TTCCCGCTTC AGTGACAACG TCGAGCACAG CTGCGCAAGG AACGCCCGTC 
     7681 GTGGCCAGCC ACGATAGCCG CGCTGCCTCG TCTTGCAGTT CATTCAGGGC ACCGGACAGG 
     7741 TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC TGCGCTGACA GCCGGAACAC GGCGGCATCA 
     7801 GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA TAGCCGAATA GCCTCTCCAC CCAAGCGGCC 
     7861 GGAGAACCTG CGTGCAATCC ATCTTGTTCA ATCATGCGAA ACGATCCTCA TCCTGTCTCT 
     7921 TGATCGATCT TTGCAAAAGC CTAGGCCTCC AAAAAAGCCT CCTCACTACT TCTGGAATAG 
     7981 CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC ATAAATAAAA AAAATTAGTC AGCCATGGGG 
     8041 CGGAGAATGG GCGGAACTGG GCGGAGTTAG GGGCGGGATG GGCGGAGTTA GGGGCGGGAC 
     8101 TATGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     8161 GGACTTTCCA CACCTGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     8221 TGGGGAGCCT GGGGACTTTC CACACCCTAA CTGACACACA TTCCACAGCT GGTTCTTTCC 
     8281 GCCTCAGGAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG 
     8341 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT 
     8401 CCCCGAAAAG TGCCACCTGA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG 
     8461 GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC 
     8521 TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC 
     8581 CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT 
     8641 GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG 
     8701 TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG 
     8761 GTCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG 
     8821 CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//