LOCUS       RC229422                8628 bp    DNA     circular  SYN 
DEFINITION  MAGEL2 (Myc-DDK-tagged)-Human MAGE-like 2 (MAGEL2). Note: ORF is codon optimized
ACCESSION   NM_019066
VERSION     NM_019066.4
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4785..4876 
                     /label="Myc-DDK"  
     misc_feature    4906..5491 
                     /label="PolyA signal"  
     misc_feature    4963..4982 
                     /label="Restriction Enzyme"  
     misc_feature    5767..6439 
                     /label="ColE1"  
     misc_feature    6869..7663 
                     /label="Kan/Neo"  
     misc_feature    7698..8000 
                     /label="SV40 ori"  
     misc_feature    8086..8563 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4767..4773 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4767 
                     /label="MAGEL2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCCCAGCTG TCCAAGAACC TGGGGGATTC CTCCCCCCCT GCTGAGGCCC 
     1081 CCAAGCCACC GGTGTACTCT AGGCCCACTG TGCTCATGCG GGCACCACCT GCATCCAGTA 
     1141 GAGCACCCCC GGTGCCCTGG GACCCCCCAC CCATAGATTT GCAGGCGTCC CTGGCAGCAT 
     1201 GGCAGGCACC CCAACCCGCC TGGGAAGCGC CCCAGGGGCA GCTGCCCGCC CCAGTCGTCC 
     1261 CAATGACCCA ACCCCCTGCC CTGGGTGGCC CTATCGTGCC CGCTCCTCCT CTTGGCGGAC 
     1321 CAATGGGAAA GCCGCCAACA CCAGGAGTGC TGATGGTGCA CCCACCCCCT CCAGGGGCCC 
     1381 CTATGGCCCA GCCCCCCACC CCAGGCGTCC TGATGGTCCA TCCAAGCGCC CCAGGTGCCC 
     1441 CGATGGCTCA CCCACCCCCT CCTGGAACCC CCATGTCCCA CCCACCCCCA CCCGGCACAC 
     1501 CTATGGCCCA CCCGCCTCCG CCTGGAACTC CCATGGCACA CCCTCCTCCA CCCGGAACTC 
     1561 CCATGGTGCA CCCGCCGCCA CCTGGGACCC CCATGGCCCA CCCCCCGCCT CCCGGAACCC 
     1621 CCATGGCTCA CCCGCCCCCC CCAGGTACAC CCATGGCCCA TCCTCCTCCT CCCGGAACCC 
     1681 CAATGGCCCA TCCGCCTCCC CCCGGGACCC CTATGGCACA GCCCCCTGCA CCAGGAGTGC 
     1741 TGATGGCCCA GCCTCTGACT CCTGGCGTAC TGATGGTGCA GCCAGCCGCG CCTGGGGCTC 
     1801 CTATGGTGCA ACCCCCCCCA GCAGCAATGA TGACCCAGCC GCAACCATCC GGAGCGCCCA 
     1861 TGGCGAAGCC CCCCGGACCC GGCGTGCTCA TGATACATCC CCCTGGCGCT AGAGCACCAA 
     1921 TGACTCAGCC TCCCGCCAGT GGCGCACCAA TGGCCCAGCC CGCCGCCCCT CCCGCCCAGC 
     1981 CCATGGCACC ACCTGCTCAG CCCATGGCTT CTTGGGCCCC CCAAGCCCAA CCCCTTATCC 
     2041 TGCAAATCCA GAGCCAGGTG ATCAGAGCAC CTCCGCAAGT TCCGCAGGGC CCGCAGGCTC 
     2101 CTCCAGCTCA ACTTGCAACT CCTCCGGGGT GGCAGGCAAC AAGTCCTGGT TGGCAGGCAA 
     2161 CCCAACAGGG CTGGCAGGCA ACACCCCTGA CATGGCAGAC AACCCAGGTA ACTTGGCAGG 
     2221 CCCCGGCGGT GACCTGGCAG GTGCCTCCCC CCATGAGGCA GGGGCCCCCC CCTATCAGGC 
     2281 CTGGCCCACC CCCCATCAGG CCTGGACCTC CCCCAGTAAG GCAAGCTCCT CCCCTTATCA 
     2341 GGCAAGCGCC ACCCGTGATA AGACAAGCGC CCCCAGTCAT CCGCCAGGCT CCACCCGTTA 
     2401 TTAGACAAGC CCCAGCCGTT ATTAGGCAGG CACCCCCCGT GATTAGGCAG GCTCCACCAG 
     2461 TGATAAGGCA GGCACCTCCA GTCATCAGAC AGGCACCACC ACTGATTCGA CAGGCTCCAC 
     2521 CTCCCATTCG GCCGGCCCCC CAGGTGCTCG CTACGCAACC TCCCCTGTGG CAGGCGCTGC 
     2581 CCCCCCCTCC GCCACTGCGA CAGGCCCCAC AGGCCCGGCT GCCTGCCCCT CAAGTGCAGG 
     2641 CCGCGCCTCA GGTCCCTACG GCACCACCAG CAACCCAGGT GCCCGCCGCA CCTCCAGCTG 
     2701 GGCCGCAGGT GCCCCAGCCT GTTCTTCCCG CACCTCTGAG CGCACCATTG TCAGCCCCCC 
     2761 AGGCAGTACA TTGTCCATCT ATAATTTGGC AAGCACCTAA AGGGCAACCA CCAGTTCCTC 
     2821 ACGAGATCCC CACCAGCATG GAATTTCAGG AGGTGCAGCA GACTCAGGCA CTCGCCTGGC 
     2881 AGGCCCAGAA AGCGCCTACC CATATCTGGC AGCCTCTGCC TGCACAGGAG GCCCAGCGAC 
     2941 AGGCTCCCCC GCTGGTGCAG CTGGAACAAC CATTCCAGGG TGCCCCACCC AGTCAAAAAG 
     3001 CCGTGCAGAT ACAGTTGCCC CCACAACAAG CCCAGGCTAG TGGCCCTCAA GCTGAGGTAC 
     3061 CCACTCTGCC TCTGCAGCCA AGTTGGCAGG CGCCCCCAGC AGTGCTGCAG GCCCAACCTG 
     3121 GTCCACCTGT CGCGGCGGCC AACTTCCCAC TCGGTAGCGC CAAGAGCCTC ATGACACCGT 
     3181 CCGGCGAGTG CCGCGCCTCA AGCATAGACA GGCGAGGTTC TTCCAAGGAG CGGAGGACAT 
     3241 CCTCAAAAGA ACGGAGAGCC CCATCTAAGG ACAGGATGAT CTTTGCCGCC ACGTTCTGCG 
     3301 CCCCCAAGGC AGTGTCTGCT GCCAGAGCAC ATTTGCCTGC CGCATGGAAG AATCTGCCTG 
     3361 CCACTCCTGA AACATTTGCG CCTTCCTCCA GCGTCTTCCC TGCCACATCT CAGTTCCAAC 
     3421 CGGCCAGCCT GAACGCTTTC AAGGGGCCAT CTGCCGCTAG CGAAACCCCC AAGAGCCTGC 
     3481 CCTACGCCTT GCAGGATCCA TTCGCTTGCG TTGAGGCTCT GCCTGCAGTA CCATGGGTCC 
     3541 CCCAGCCAAA TATGAATGCC TCCAAGGCTT CACAAGCAGT GCCAACGTTC TTGATGGCAA 
     3601 CCGCAGCCGC TCCCCAGGCA ACTGCCACTA CTCAGGAGGC CTCCAAAACA TCTGTGGAAC 
     3661 CCCCCCGCCG GTCTGGTAAG GCTACCAGAA AGAAAAAGCA TCTGGAGGCC CAGGAAGATT 
     3721 CCCGCGGGCA CACATTGGCT TTCCACGACT GGCAGGGCCC TCGCCCATGG GAAAACTTGA 
     3781 ACTTGTCCGA CTGGGAGGTC CAGTCTCCAA TTCAAGTTAG CGGAGATTGG GAACACCCGA 
     3841 ACACTCCAAG AGGGCTGTCC GGCTGGGAGG GACCCTCCAC ATCAAGAATA CTGAGCGGCT 
     3901 GGGAGGGTCC CTCAGCCTCT TGGGCCCTTT CTGCATGGGA GGGGCCTAGC ACCAGCCGCG 
     3961 CCCTTGGGTT GAGCGAATCT CCTGGGTCAT CTCTTCCCGT AGTCGTCTCT GAAGTGGCAA 
     4021 GCGTCTCACC TGGAAGCTCC GCCACACAGG ACAATTCCAA GGTGGAGGCT CAACCTCTGA 
     4081 GTCCCCTGGA CGAGCGCGCT AATGCCCTTG TGCAGTTTCT GCTGGTGAAG GACCAAGCAA 
     4141 AGGTTCCCGT ACAGCGAAGC GAAATGGTTA AAGTAATTCT GAGAGAGTAC AAGGACGAGT 
     4201 GCTTGGACAT AATAAATAGA GCCAACAATA AACTGGAATG CGCTTTCGGT TATCAACTCA 
     4261 AGGAGATCGA CACGAAGAAT CACGCCTATA TAATTATTAA CAAACTGGGC TACCATACGG 
     4321 GAAATCTTGT GGCCTCATAT CTGGATAGGC CCAAATTCGG CCTCCTCATG GTCGTTCTGT 
     4381 CTCTGATTTT CATGAAGGGA AACTGCGTGA GGGAGGATCT CATCTTCAAT TTCCTGTTCA 
     4441 AGCTTGGCCT CGACGTGCGG GAGACTAATG GGCTGTTTGG AAACACCAAG AAACTGATAA 
     4501 CCGAAGTGTT CGTCAGACAA AAATATCTGG AGTACCGCCG GATCCCTTAC ACTGAACCCG 
     4561 CTGAGTACGA ATTTCTGTGG GGACCAAGGG CTTTCCTTGA GACATCCAAG ATGCTGGTAC 
     4621 TCAGATTTCT GGCCAAACTG CATAAGAAGG ACCCTCAGTC CTGGCCTTTT CACTATCTGG 
     4681 AGGCCCTCGC AGAGTGCGAA TGGGAAGATA CGGATGAGGA TGAGCCTGAC ACTGGGGATT 
     4741 CTGCCCATGG CCCCACATCC AGGCCACCCC CTCGGACGCG TACGCGGCCG CTCGAGCAGA 
     4801 AACTCATCTC AGAAGAGGAT CTGGCAGCAA ATGATATCCT GGATTACAAG GATGACGACG 
     4861 ATAAGGTTTA AACGGCCGGC CGCGGTCATA GCTGTTTCCT GAACAGATCC CGGGTGGCAT 
     4921 CCCTGTGACC CCTCCCCAGT GCCTCTCCTG GCCCTGGAAG TTGCCACTCC AGTGCCCACC 
     4981 AGCCTTGTCC TAATAAAATT AAGTTGCATC ATTTTGTCTG ACTAGGTGTC CTTCTATAAT 
     5041 ATTATGGGGT GGAGGGGGGT GGTATGGAGC AAGGGGCAAG TTGGGAAGAC AACCTGTAGG 
     5101 GCCTGCGGGG TCTATTGGGA ACCAAGCTGG AGTGCAGTGG CACAATCTTG GCTCACTGCA 
     5161 ATCTCCGCCT CCTGGGTTCA AGCGATTCTC CTGCCTCAGC CTCCCGAGTT GTTGGGATTC 
     5221 CAGGCATGCA TGACCAGGCT CAGCTAATTT TTGTTTTTTT GGTAGAGACG GGGTTTCACC 
     5281 ATATTGGCCA GGCTGGTCTC CAACTCCTAA TCTCAGGTGA TCTACCCACC TTGGCCTCCC 
     5341 AAATTGCTGG GATTACAGGC GTGAACCACT GCTCCCTTCC CTGTCCTTCT GATTTTAAAA 
     5401 TAACTATACC AGCAGGAGGA CGTCCAGACA CAGCATAGGC TACCTGGCCA TGCCCAACCG 
     5461 GTGGGACATT TGAGTTGCTT GCTTGGCACT GTCCTCTCAT GCGTTGGGTC CACTCAGTAG 
     5521 ATGCCTGTTG AATTGGGTAC GCGGCCAGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT 
     5581 AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA 
     5641 GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC 
     5701 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT 
     5761 ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT 
     5821 GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG 
     5881 CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA 
     5941 CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA 
     6001 CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC 
     6061 GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG 
     6121 AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG 
     6181 TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA 
     6241 GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC 
     6301 TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG 
     6361 GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA 
     6421 TGAGTAACCT GAGGCTATGG CAGGGCCTGC CGCCCCGACG TTGGCTGCGA GCCCTGGGCC 
     6481 TTCACCCGAA CTTGGGGGGT GGGGTGGGGA AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA 
     6541 ATGGGGTCTC GGTGGGGTAT CGACAGAGTG CCAGCCCTGG GACCGAACCC CGCGTTTATG 
     6601 AACAAACGAC CCAACACCGT GCGTTTTATT CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT 
     6661 TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA 
     6721 CTCCAGCATG AGATCCCCGC GCTGGAGGAT CATCCAGCCG GCGTCCCGGA AAACGATTCC 
     6781 GAAGCCCAAC CTTTCATAGA AGGCGGCGGT GGAATCGAAA TCTCGTGATG GCAGGTTGGG 
     6841 CGTCGCTTGG TCGGTCATTT CGAACCCCAG AGTCCCGCTC AGAAGAACTC GTCAAGAAGG 
     6901 CGATAGAAGG CGATGCGCTG CGAATCGGGA GCGGCGATAC CGTAAAGCAC GAGGAAGCGG 
     6961 TCAGCCCATT CGCCGCCAAG CTCTTCAGCA ATATCACGGG TAGCCAACGC TATGTCCTGA 
     7021 TAGCGATCCG CCACACCCAG CCGGCCACAG TCGATGAATC CAGAAAAGCG GCCATTTTCC 
     7081 ACCATGATAT TCGGCAAGCA GGCATCGCCA TGGGTCACGA CGAGATCCTC GCCGTCGGGC 
     7141 ATGCTCGCCT TGAGCCTGGC GAACAGTTCG GCTGGCGCGA GCCCCTGATG CTCTTCGTCC 
     7201 AGATCATCCT GATCGACAAG ACCGGCTTCC ATCCGAGTAC GTGCTCGCTC GATGCGATGT 
     7261 TTCGCTTGGT GGTCGAATGG GCAGGTAGCC GGATCAAGCG TATGCAGCCG CCGCATTGCA 
     7321 TCAGCCATGA TGGATACTTT CTCGGCAGGA GCAAGGTGAG ATGACAGGAG ATCCTGCCCC 
     7381 GGCACTTCGC CCAATAGCAG CCAGTCCCTT CCCGCTTCAG TGACAACGTC GAGCACAGCT 
     7441 GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC GATAGCCGCG CTGCCTCGTC TTGCAGTTCA 
     7501 TTCAGGGCAC CGGACAGGTC GGTCTTGACA AAAAGAACCG GGCGCCCCTG CGCTGACAGC 
     7561 CGGAACACGG CGGCATCAGA GCAGCCGATT GTCTGTTGTG CCCAGTCATA GCCGAATAGC 
     7621 CTCTCCACCC AAGCGGCCGG AGAACCTGCG TGCAATCCAT CTTGTTCAAT CATGCGAAAC 
     7681 GATCCTCATC CTGTCTCTTG ATCGATCTTT GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC 
     7741 TCACTACTTC TGGAATAGCT CAGAGGCCGA GGCGGCCTCG GCCTCTGCAT AAATAAAAAA 
     7801 AATTAGTCAG CCATGGGGCG GAGAATGGGC GGAACTGGGC GGAGTTAGGG GCGGGATGGG 
     7861 CGGAGTTAGG GGCGGGACTA TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     7921 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCTGGTTGCT GACTAATTGA GATGCATGCT 
     7981 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCCTAACT GACACACATT 
     8041 CCACAGCTGG TTCTTTCCGC CTCAGGACTC TTCCTTTTTC AATATTATTG AAGCATTTAT 
     8101 CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA 
     8161 GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG CGCCCTGTAG CGGCGCATTA 
     8221 AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG 
     8281 CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA 
     8341 GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC 
     8401 AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT 
     8461 CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA 
     8521 ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC 
     8581 TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTT
//