LOCUS       RC229419                8556 bp    DNA     circular  SYN 
DEFINITION  NHSL2 (Myc-DDK-tagged)-Human NHS-like 2 (NHSL2)
ACCESSION   NM_001013627
VERSION     NM_001013627.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4713..4804 
                     /label="Myc-DDK"  
     misc_feature    4834..5419 
                     /label="PolyA signal"  
     misc_feature    4891..4910 
                     /label="Restriction Enzyme"  
     misc_feature    5695..6367 
                     /label="ColE1"  
     misc_feature    6797..7591 
                     /label="Kan/Neo"  
     misc_feature    7626..7928 
                     /label="SV40 ori"  
     misc_feature    8014..8491 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4695..4701 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4695 
                     /label="NHSL2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCGTTCTAC AGGCGCACGG TGGTACCCCA GCGCCTGTGC CCGCGCAACC 
     1081 CGCCGCAGCA GCTGGCGGAG CTCCGCGACG TGAGCCACCT GGCAGCGCTC AGCCTGCTCC 
     1141 GGCAGCTCGC CGACCTCTGT GGCCACTCGT TGGCTCTGCT CGAGGACCTC GAGGGGCACC 
     1201 TGCTGGCCCT GGGGCGCCGC ACAGACAGCC TGTACCGGCG CACCGTGCGC CTCCGCCGCC 
     1261 GCCTTCCCTG CCGCCTGCTT GGCCCGGAGG AGGACGAGGA AGAGCTAGCT GCAGCTAACT 
     1321 CGGGTCGGGA AAATGCGACA GCGACTGCCC ACTCGAGGTC GTCATGGCGA CAGCCAGTGA 
     1381 ACGTGTTCCT CTCCTCGGGC AGGCCCCCGA GTGTAGAGGA GCTGCTTCGG GAGGCGCAGC 
     1441 TCAATCTCCA GAGCCTGTTG CAAGAAGAAT ATGAGGAACA GTACTCGGAG GCCAGACTTG 
     1501 TGGGGCAGAC CTTCCGCTCT TCTGATGAGG CCACTAAGCC CACCCCCAAC CCAAGGCCCC 
     1561 AGTCTGCCAG GCGTCTGGAG TTTATATTGA TGCCTACAAA ACGGCAGCTG AGCGAGGATG 
     1621 AGACTACCAC CCAGGGTGTG AGGGCCCCCG AGGCCTCCCT GAGCCTGTCT ACCACAGCCG 
     1681 ACAAGCAAAC TGCCTGGAAT AGCCTTTTCC CTCTGCCCAT CCTAGAGGAG AAGCGGTGGC 
     1741 CTCAGCTTTG CTCCACGCAG TCTGACATTG TGCCCATCAA CATCTCTGGG CAGCAGTTTG 
     1801 ATAAACATGC AAGTTTGCGA CACTCGTTGT TTAACACAGA GACAGCCGTG AACCCCAAGT 
     1861 CCACCCTGAG GCGGAGGCGG ACCATTATTG GATTCTCTAA CTTTTCCCAG CGAGACCAAG 
     1921 GTCACAGCAA CAGCCCAGCA GGCAGTGTGG CCCACTCTAC CACCTCCGAC ATCAGGCCCA 
     1981 GTCACTCAGT TCCAGAAGGG GTTCATGGAA GAGTTGCAGT TGGTCAGGAT GCTCGGTTCC 
     2041 CAAGTCTCAC CTCGCCAGTA CTGAGAACTC CTTCCAGTGA GCCGGACGAA CCTCACCAGG 
     2101 CACGGAGTGG CCCAAACCCT CCTGGCATGG AGAGCATGGG AATGGTGTAC AGTGTCCCCA 
     2161 GTTCTTGCAA TGGACCTACA GAATCAACCT TCTCCACTTC CTGGAAGGGA GATGCTTTTA 
     2221 CCTACATGAC TCCAAGTGCC ACCAGCCAGA GCAATCAAGT CAATGAAAAT GGGAAAAATC 
     2281 CTTCCTGTGG GAATTCTTGG GTCTCTCTAA ACAAAGTCCC ACCTCTGGTT CCTAAGGAGG 
     2341 CTGCTACCCT CCTTGTCGCT CGTGATAACC CAGCAGGATG CAGTGGGTCA GCTGGCTACC 
     2401 CTGAGCGCCT TATTCAGCAA AGGCACATGC CCGAAAGACC CTCCAAGATT GGCCTTCTGA 
     2461 CCAGTGGGAC CTCGAGGCTG GAGACAGGCC CCGGTGGGGC CAGCAGATTC CGGGAGCGGT 
     2521 CACTGTCTGT GCCCACAGAC TCAGGCACCA CAGATGTGGA CTATGATGAG GAGCAGAAGG 
     2581 CCAATGAGGC CTGTGCCCTG CCTTTTGCCA GTACGAGCTC TGAGGGCAGT AACAGTGCTG 
     2641 ACAACATTGC CTCCCTTAGT GCCCAGCAAG AGGCCCAGCA CAGAAGGCAG AGGTCCAAGA 
     2701 GTATCTCACT TAGGAAGGCC AAAAAGAAGC CTTCCCCACC CACACGCAGT GTCTCACTGG 
     2761 TCAAAGATGA GCCAGGCCTC TTGCCTGAAG GTGGGTCAGC ACTACCCAAG GACCAGAGGC 
     2821 CCAAGAGCCT TTGCCTCTCC TTGGAACATC AAGGACATCA CTCGTCCCAC CCAGATGCTC 
     2881 AGGGTCACCC AGCTATTCCA AACCACAAAG ATCCAGAAAG TACACAATTC TCCCACCACT 
     2941 GGTATCTTAC TGACTGGAAG TCTGGTGACA CCTACCAATC CCTGTCCAGC TCCAGCACTG 
     3001 CCACTGGCAC CACAGTCATT GAGTGCACCC AAGTTCAGGG CAGCTCAGAG TCTCTTGCCT 
     3061 CACCTTCCAC CTCCAGAGCC ACTACACCTT CCCAACTCTC CATTGAAGTG GAGGCCAGGG 
     3121 AGATATCATC CCCGGGAAGG CCCCCTGGAC TGATGTCACC CTCCAGTGGC TACTCCAGCC 
     3181 AGTCGGAAAC ACCAACACCC ACTGTTTCCA TGTCCCTGAC CCTGGGCCAC TTACCCCCTC 
     3241 CAAGCAGCAG TGTCCGGGTA CGTCCAGTGG TACCTGAGAG GAAGTCATCA CTACCCCCGA 
     3301 CGTCACCAAT GGAGAAATTT CCCAAGTCAC GGCTATCATT TGACCTACCA CTGACCTCTT 
     3361 CACCCAACCT GGATCTGTCT GGGATGAGTA TCTCCATCCG AAGCAAAACT AAGGTGAGTC 
     3421 GGCACCACTC AGAGACAAAT TTTGGCGTCA AGCTGGCCCA GAAAACTAAT CCCAACCAGC 
     3481 CAATCATGCC TATGGTTACT CAGTCCGACC TACGTTCTGT TCGCCTGAGG TCGGTCAGCA 
     3541 AGTCTGAGCC GGAAGATGAT ATTGAGAGCC CTGAGTATGC CGAGGAACCC AGAGCAGAAG 
     3601 AAGTCTTCAC CTTGCCAGAG AGAAAGACAA AACCTCCCGT AGCTGAGAAG CCTCCGGTGG 
     3661 CCCGGAGGCC TCCAAGCTTG GTCCACAAGC CACCATCTGT TCCTGAGGAG TATGCACTAA 
     3721 CTTCACCAAC CTTGGCTATG CCCCCCAGGA GCTCAATTCA ACATGCGAGA CCACTCCCTC 
     3781 AAGACAGCTA CACGGTAGTG CGGAAACCAA AGCCCTCCAG CTTCCCAGAT GGCAGAAGCC 
     3841 CAGGGGAGTC AACAGCACCC TCATCTCTTG TTTTCACGCC TTTTGCCAGT TCCTCTGATG 
     3901 CTTTCTTCTC AGGAACACAG CAGCCTCCCC AGGGAAGTGT AGAGGACGAG GGCCCCAAGG 
     3961 TGAGGGTTCT GCCTGAAAGA ATTAGCCTCC AGAGCCAGGA AGAAGCTGAG AAAAAGAAAG 
     4021 GCAAGATTCC ACCTCCCGTA CCAAAAAAAC CCAGCGTGCT GTACCTGCCT CTCACTTCTC 
     4081 CCACAGCTCA AATGGAGGCC TATGTGGCAG AACCAAGGCT GCCTCTCAGC CCCATCATCA 
     4141 CCCTGGAGGA AGACACCAAG TGTCCCGCCA CCGGCGATGA CCTGCAATCA CTTGGTCAAA 
     4201 GGGTGACTTC AACTCCTCAG GCTGACAGTG AAAGGGAGGC AAGCCCTCTG GGGAGTTCTG 
     4261 TGGAACCAGG CACCGAAGAA AAAAGTTTAA TCAGTGATAA AACAGCTGAA TGGATTGCAG 
     4321 AGGATGATGA TGACGTGTTT GTGGCTTCAC GCACAACTGA AGATTTATTT ACTGTGATAC 
     4381 ACAGGTCCAA AAGGAAGCTG CTCGGCTGGA AGGAACCTGG TGAGGCCTTT GTGGGTGGCA 
     4441 GAACGAGTTC CCACTCACCA ATAAAGAACA CAGCTGAGTC TCCAATCAGT GAGTCTACCG 
     4501 CCACTGCAGG GTCAGGCAGC AGTGCCAACC TAGATGCTGG CAGAAATGAC GATTTCAAGG 
     4561 CCTTGCTACA GAAGAAGGGA AGTAAGGCAA CTCCAAGGTC TCGTCCCTCA GCAGCTGAAC 
     4621 TTCTGAAGAC CACTAACCCA CTGGCTCGGA GAATTATTGC ACAATTTTCA AAAGACTATG 
     4681 AAACCACCGA TAACCCCAGT ACCACGCGTA CGCGGCCGCT CGAGCAGAAA CTCATCTCAG 
     4741 AAGAGGATCT GGCAGCAAAT GATATCCTGG ATTACAAGGA TGACGACGAT AAGGTTTAAA 
     4801 CGGCCGGCCG CGGTCATAGC TGTTTCCTGA ACAGATCCCG GGTGGCATCC CTGTGACCCC 
     4861 TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT GCCACTCCAG TGCCCACCAG CCTTGTCCTA 
     4921 ATAAAATTAA GTTGCATCAT TTTGTCTGAC TAGGTGTCCT TCTATAATAT TATGGGGTGG 
     4981 AGGGGGGTGG TATGGAGCAA GGGGCAAGTT GGGAAGACAA CCTGTAGGGC CTGCGGGGTC 
     5041 TATTGGGAAC CAAGCTGGAG TGCAGTGGCA CAATCTTGGC TCACTGCAAT CTCCGCCTCC 
     5101 TGGGTTCAAG CGATTCTCCT GCCTCAGCCT CCCGAGTTGT TGGGATTCCA GGCATGCATG 
     5161 ACCAGGCTCA GCTAATTTTT GTTTTTTTGG TAGAGACGGG GTTTCACCAT ATTGGCCAGG 
     5221 CTGGTCTCCA ACTCCTAATC TCAGGTGATC TACCCACCTT GGCCTCCCAA ATTGCTGGGA 
     5281 TTACAGGCGT GAACCACTGC TCCCTTCCCT GTCCTTCTGA TTTTAAAATA ACTATACCAG 
     5341 CAGGAGGACG TCCAGACACA GCATAGGCTA CCTGGCCATG CCCAACCGGT GGGACATTTG 
     5401 AGTTGCTTGC TTGGCACTGT CCTCTCATGC GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA 
     5461 TTGGGTACGC GGCCAGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC 
     5521 CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG 
     5581 GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA 
     5641 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA 
     5701 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG 
     5761 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG 
     5821 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT 
     5881 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA 
     5941 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG 
     6001 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT 
     6061 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC 
     6121 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG 
     6181 CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG 
     6241 GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA 
     6301 GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAACCTGA 
     6361 GGCTATGGCA GGGCCTGCCG CCCCGACGTT GGCTGCGAGC CCTGGGCCTT CACCCGAACT 
     6421 TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG 
     6481 TGGGGTATCG ACAGAGTGCC AGCCCTGGGA CCGAACCCCG CGTTTATGAA CAAACGACCC 
     6541 AACACCGTGC GTTTTATTCT GTCTTTTTAT TGCCGTCATA GCGCGGGTTC CTTCCGGTAT 
     6601 TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC CCTAGGGTGG GCGAAGAACT CCAGCATGAG 
     6661 ATCCCCGCGC TGGAGGATCA TCCAGCCGGC GTCCCGGAAA ACGATTCCGA AGCCCAACCT 
     6721 TTCATAGAAG GCGGCGGTGG AATCGAAATC TCGTGATGGC AGGTTGGGCG TCGCTTGGTC 
     6781 GGTCATTTCG AACCCCAGAG TCCCGCTCAG AAGAACTCGT CAAGAAGGCG ATAGAAGGCG 
     6841 ATGCGCTGCG AATCGGGAGC GGCGATACCG TAAAGCACGA GGAAGCGGTC AGCCCATTCG 
     6901 CCGCCAAGCT CTTCAGCAAT ATCACGGGTA GCCAACGCTA TGTCCTGATA GCGATCCGCC 
     6961 ACACCCAGCC GGCCACAGTC GATGAATCCA GAAAAGCGGC CATTTTCCAC CATGATATTC 
     7021 GGCAAGCAGG CATCGCCATG GGTCACGACG AGATCCTCGC CGTCGGGCAT GCTCGCCTTG 
     7081 AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC CCCTGATGCT CTTCGTCCAG ATCATCCTGA 
     7141 TCGACAAGAC CGGCTTCCAT CCGAGTACGT GCTCGCTCGA TGCGATGTTT CGCTTGGTGG 
     7201 TCGAATGGGC AGGTAGCCGG ATCAAGCGTA TGCAGCCGCC GCATTGCATC AGCCATGATG 
     7261 GATACTTTCT CGGCAGGAGC AAGGTGAGAT GACAGGAGAT CCTGCCCCGG CACTTCGCCC 
     7321 AATAGCAGCC AGTCCCTTCC CGCTTCAGTG ACAACGTCGA GCACAGCTGC GCAAGGAACG 
     7381 CCCGTCGTGG CCAGCCACGA TAGCCGCGCT GCCTCGTCTT GCAGTTCATT CAGGGCACCG 
     7441 GACAGGTCGG TCTTGACAAA AAGAACCGGG CGCCCCTGCG CTGACAGCCG GAACACGGCG 
     7501 GCATCAGAGC AGCCGATTGT CTGTTGTGCC CAGTCATAGC CGAATAGCCT CTCCACCCAA 
     7561 GCGGCCGGAG AACCTGCGTG CAATCCATCT TGTTCAATCA TGCGAAACGA TCCTCATCCT 
     7621 GTCTCTTGAT CGATCTTTGC AAAAGCCTAG GCCTCCAAAA AAGCCTCCTC ACTACTTCTG 
     7681 GAATAGCTCA GAGGCCGAGG CGGCCTCGGC CTCTGCATAA ATAAAAAAAA TTAGTCAGCC 
     7741 ATGGGGCGGA GAATGGGCGG AACTGGGCGG AGTTAGGGGC GGGATGGGCG GAGTTAGGGG 
     7801 CGGGACTATG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     7861 GCCTGGGGAC TTTCCACACC TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     7921 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCCTAACTGA CACACATTCC ACAGCTGGTT 
     7981 CTTTCCGCCT CAGGACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT 
     8041 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC 
     8101 ACATTTCCCC GAAAAGTGCC ACCTGACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT 
     8161 GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC 
     8221 GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG 
     8281 GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT 
     8341 TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG 
     8401 TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT 
     8461 ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA 
     8521 AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTT
//