LOCUS       RC229412                8358 bp    DNA     circular  SYN 
DEFINITION  SORCS2 (Myc-DDK-tagged)-Human sortilin-related VPS10 domain containing receptor 2 (SORCS2)
ACCESSION   NM_020777
VERSION     NM_020777.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4515..4606 
                     /label="Myc-DDK"  
     misc_feature    4636..5221 
                     /label="PolyA signal"  
     misc_feature    4693..4712 
                     /label="Restriction Enzyme"  
     misc_feature    5497..6169 
                     /label="ColE1"  
     misc_feature    6599..7393 
                     /label="Kan/Neo"  
     misc_feature    7428..7730 
                     /label="SV40 ori"  
     misc_feature    7816..8293 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4497..4503 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4497 
                     /label="SORCS2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGCACCGG GGGCCCTCGC GCGCCTCGAA GGGCCCCGGC CCCACCGCCC 
     1081 GAGCCCCGAG CCCCGGGGCT CCGCCGCCGC CGCGCTCGCC GCGCTCGCGG CCGCTCCTGC 
     1141 TGCTGCTGCT GCTGCTGGGC GCCTGCGGGG CGGCGGGGCG CTCCCCTGAG CCCGGGCGCC 
     1201 TGGGTCCTCA CGCCCAACTG ACCCGGGTGC CGCGGAGCCC TCCCGCGGGG CGCGCGGAGC 
     1261 CCGGTGGCGG CGAGGACCGG CAGGCGCGCG GCACGGAGCC AGGCGCCCCG GGTCCGAGTC 
     1321 CCGGTCCCGC TCCTGGTCCC GGCGAGGACG GCGCCCCCGC CGCGGGCTAC CGGCGCTGGG 
     1381 AGCGGGCGGC GCCGCTGGCC GGAGTGGCTT CGCGGGCGCA GGTCTCGCTC ATCAGCACGT 
     1441 CGTTCGTGCT CAAGGGGGAC GCGACGCACA ACCAGGCGAT GGTGCACTGG ACGGGCGAGA 
     1501 ACAGCAGCGT GATCTTGATC CTGACGAAGT ACTACCACGC AGACATGGGG AAGGTTCTGG 
     1561 AAAGTTCTCT GTGGCGGTCA TCAGATTTCG GGACGTCCTA CACCAAGCTC ACCCTCCAGC 
     1621 CTGGTGTCAC CACCGTCATC GACAATTTCT ACATCTGCCC GACCAACAAG AGGAAGGTCA 
     1681 TCCTTGTCAG CTCCTCACTC AGTGACCGGG ACCAGAGCCT ATTCCTCAGC GCAGACGAAG 
     1741 GCGCCACCTT TCAGAAGCAG CCCATTCCCT TCTTCGTGGA AACTCTGATT TTCCACCCTA 
     1801 AGGAGGAGGA CAAGGTCCTC GCCTACACAA AGGAGAGCAA GCTCTACGTG TCATCTGACT 
     1861 TGGGGAAAAA GTGGACACTT CTGCAAGAGC GAGTGACCAA AGACCACGTG TTCTGGTCTG 
     1921 TGTCTGGGGT GGACGCTGAC CCTGACTTGG TCCACGTGGA AGCCCAAGAC CTCGGTGGAG 
     1981 ATTTTCGGTA CGTCACCTGC GCAATCCACA ATTGCTCCGA GAAGATGCTG ACAGCCCCAT 
     2041 TCGCAGGCCC CATTGACCAC GGGTCTCTGA CCGTGCAGGA CGATTACATC TTCTTTAAGG 
     2101 CAACATCAGC AAACCAGACA AAATACTACG TCTCTTATCG TCGAAATGAA TTTGTCCTGA 
     2161 TGAAGCTGCC GAAGTATGCA TTGCCAAAGG ATCTGCAGAT CATCAGCACG GACGAGAGTC 
     2221 AGGTGTTCGT GGCGGTGCAG GAGTGGTACC AGATGGACAC CTACAACCTG TACCAGTCGG 
     2281 ACCCACGGGG CGTGCGCTAC GCGCTGGTGC TGCAGGACGT GCGCAGCTCA CGGCAGGCGG 
     2341 AGGAGAGCGT GCTCATCGAC ATCCTGGAGG TCAGAGGGGT GAAAGGAGTC TTCCTGGCAA 
     2401 ACCAAAAAAT TGATGGGAAA GTGATGACGC TTATAACCTA CAACAAGGGC CGCGACTGGG 
     2461 ATTACCTGAG GCCACCCAGC ATGGACATGA ATGGAAAACC AACCAACTGC AAGCCTCCAG 
     2521 ACTGCCACCT GCACCTGCAC CTGCGCTGGG CAGACAACCC CTACGTATCA GGCACCGTGC 
     2581 ACACCAAGGA CACCGCCCCA GGCCTCATCA TGGGTGCAGG TAACCTGGGC TCACAGCTGG 
     2641 TGGAATATAA AGAAGAAATG TACATCACGT CAGACTGTGG TCACACCTGG CGGCAGGTGT 
     2701 TTGAGGAAGA GCATCACATC CTGTACCTGG ACCACGGCGG CGTGATCGTG GCCATCAAAG 
     2761 ACACCTCCAT CCCTTTGAAG ATCCTCAAGT TCAGTGTGGA CGAGGGCCTC ACCTGGAGCA 
     2821 CGCACAACTT CACCAGCACC TCGGTGTTTG TGGACGGGCT GCTGAGTGAG CCAGGGGACG 
     2881 AGACGCTGGT CATGACGGTC TTTGGCCACA TCAGCTTCCG CTCCGATTGG GAGCTGGTCA 
     2941 AGGTGGACTT CCGGCCCTCA TTCTCCAGGC AGTGCGGCGA GGAGGACTAC AGCTCCTGGG 
     3001 AGCTCTCCAA CCTGCAGGGC GACCGCTGTA TCATGGGCCA GCAGAGAAGT TTCCGGAAAA 
     3061 GAAAGTCCAC GTCCTGGTGC ATCAAGGGGA GGAGCTTCAC GTCGGCGCTC ACGTCCCGCG 
     3121 TGTGCGAGTG CCGGGACTCG GACTTCCTGT GCGACTACGG ATTTGAGCGC TCCTCCTCCT 
     3181 CAGAGTCCAG CACCAACAAG TGCTCTGCCA ACTTCTGGTT TAACCCATTG TCCCCGCCTG 
     3241 ACGACTGTGC CCTGGGCCAG ACCTACACCA GCAGCCTTGG GTACCGGAAA GTGGTGTCCA 
     3301 ACGTGTGTGA GGGTGGGGTG GACATGCAGC AGAGTCAGGT GCAGCTGCAG TGCCCCCTCA 
     3361 CGCCGCCCCG GGGCCTGCAG GTCAGCATTC AAGGCGAGGC GGTGGCCGTG CGGCCTGGAG 
     3421 AGGACGTCCT GTTTGTGGTG CGGCAGGAGC AGGGTGATGT CCTGACTACC AAGTACCAGG 
     3481 TAGACCTTGG GGACGGCTTC AAGGCCATGT ACGTGAACCT TACACTGACC GGGGAGCCCA 
     3541 TCCGGCACCG CTACGAGAGC CCCGGCATCT ACCGCGTGTC CGTCAGGGCA GAGAACACGG 
     3601 CAGGCCACGA TGAGGCGGTG CTCTTTGTCC AGGTCAACTC CCCCCTGCAG GCCCTCTACC 
     3661 TGGAGGTGGT TCCTGTCATT GGCCTCAACC AGGAGGTGAA CCTCACAGCT GTGCTGCTTC 
     3721 CCTTGAACCC TAACCTCACC GTCTTCTACT GGTGGATCGG CCACAGCCTG CAGCCCCTCC 
     3781 TTTCCCTGGA TAATTCTGTG ACAACGCGGT TTTCGGACAC GGGCGACGTG CGTGTGACGG 
     3841 TGCAGGCCGC CTGTGGGAAC TCGGTGCTGC AGGACTCCAG GGTCCTCCGT GTGCTGGATC 
     3901 AATTTCAAGT CATGCCTCTG CAGTTTTCCA AGGAGCTGGA TGCCTACAAC CCCAACACCC 
     3961 CTGAGTGGAG GGAAGACGTG GGCCTGGTGG TCACCCGGCT GCTCTCCAAG GAGACCAGCG 
     4021 TCCCTCAGGA GCTTCTGGTG ACTGTGGTGA AGCCGGGGCT GCCCACTTTG GCCGATCTGT 
     4081 ACGTGCTCCT GCCCCCTCCC AGGCCCACAA GGAAGAGGAG CCTCTCGAGT GATAAGAGGC 
     4141 TCGCCGCCAT CCAGCAGGTG CTGAACGCAC AGAAGATCAG CTTCCTCCTG CGAGGCGGAG 
     4201 TCCGGGTCCT GGTGGCCCTG CGGGACACAG GCACAGGTGC TGAGCAGCTG GGCGGCGGTG 
     4261 GCGGCTACTG GGCGGTAGTG GTGCTGTTTG TCATCGGGCT CTTCGCAGCG GGAGCCTTCA 
     4321 TCCTCTACAA GTTCAAAAGG AAACGGCCAG GCAGGACCGT GTACGCCCAA ATGCACAACG 
     4381 AGAAGGAGCA GGAGATGACC AGCCCTGTGA GTCACAGTGA GGACGTCCAG GGCGCTGTCC 
     4441 AGGGCAACCA CTCAGGCGTG GTCCTGAGCA TCAACTCCCG AGAGATGCAC AGCTACCTGG 
     4501 TGAGCACGCG TACGCGGCCG CTCGAGCAGA AACTCATCTC AGAAGAGGAT CTGGCAGCAA 
     4561 ATGATATCCT GGATTACAAG GATGACGACG ATAAGGTTTA AACGGCCGGC CGCGGTCATA 
     4621 GCTGTTTCCT GAACAGATCC CGGGTGGCAT CCCTGTGACC CCTCCCCAGT GCCTCTCCTG 
     4681 GCCCTGGAAG TTGCCACTCC AGTGCCCACC AGCCTTGTCC TAATAAAATT AAGTTGCATC 
     4741 ATTTTGTCTG ACTAGGTGTC CTTCTATAAT ATTATGGGGT GGAGGGGGGT GGTATGGAGC 
     4801 AAGGGGCAAG TTGGGAAGAC AACCTGTAGG GCCTGCGGGG TCTATTGGGA ACCAAGCTGG 
     4861 AGTGCAGTGG CACAATCTTG GCTCACTGCA ATCTCCGCCT CCTGGGTTCA AGCGATTCTC 
     4921 CTGCCTCAGC CTCCCGAGTT GTTGGGATTC CAGGCATGCA TGACCAGGCT CAGCTAATTT 
     4981 TTGTTTTTTT GGTAGAGACG GGGTTTCACC ATATTGGCCA GGCTGGTCTC CAACTCCTAA 
     5041 TCTCAGGTGA TCTACCCACC TTGGCCTCCC AAATTGCTGG GATTACAGGC GTGAACCACT 
     5101 GCTCCCTTCC CTGTCCTTCT GATTTTAAAA TAACTATACC AGCAGGAGGA CGTCCAGACA 
     5161 CAGCATAGGC TACCTGGCCA TGCCCAACCG GTGGGACATT TGAGTTGCTT GCTTGGCACT 
     5221 GTCCTCTCAT GCGTTGGGTC CACTCAGTAG ATGCCTGTTG AATTGGGTAC GCGGCCAGCG 
     5281 GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA 
     5341 CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC 
     5401 GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC 
     5461 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG 
     5521 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT 
     5581 CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA 
     5641 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC 
     5701 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC 
     5761 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT 
     5821 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT 
     5881 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC 
     5941 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA 
     6001 AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 
     6061 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 
     6121 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAACCT GAGGCTATGG CAGGGCCTGC 
     6181 CGCCCCGACG TTGGCTGCGA GCCCTGGGCC TTCACCCGAA CTTGGGGGGT GGGGTGGGGA 
     6241 AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA ATGGGGTCTC GGTGGGGTAT CGACAGAGTG 
     6301 CCAGCCCTGG GACCGAACCC CGCGTTTATG AACAAACGAC CCAACACCGT GCGTTTTATT 
     6361 CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC 
     6421 AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA CTCCAGCATG AGATCCCCGC GCTGGAGGAT 
     6481 CATCCAGCCG GCGTCCCGGA AAACGATTCC GAAGCCCAAC CTTTCATAGA AGGCGGCGGT 
     6541 GGAATCGAAA TCTCGTGATG GCAGGTTGGG CGTCGCTTGG TCGGTCATTT CGAACCCCAG 
     6601 AGTCCCGCTC AGAAGAACTC GTCAAGAAGG CGATAGAAGG CGATGCGCTG CGAATCGGGA 
     6661 GCGGCGATAC CGTAAAGCAC GAGGAAGCGG TCAGCCCATT CGCCGCCAAG CTCTTCAGCA 
     6721 ATATCACGGG TAGCCAACGC TATGTCCTGA TAGCGATCCG CCACACCCAG CCGGCCACAG 
     6781 TCGATGAATC CAGAAAAGCG GCCATTTTCC ACCATGATAT TCGGCAAGCA GGCATCGCCA 
     6841 TGGGTCACGA CGAGATCCTC GCCGTCGGGC ATGCTCGCCT TGAGCCTGGC GAACAGTTCG 
     6901 GCTGGCGCGA GCCCCTGATG CTCTTCGTCC AGATCATCCT GATCGACAAG ACCGGCTTCC 
     6961 ATCCGAGTAC GTGCTCGCTC GATGCGATGT TTCGCTTGGT GGTCGAATGG GCAGGTAGCC 
     7021 GGATCAAGCG TATGCAGCCG CCGCATTGCA TCAGCCATGA TGGATACTTT CTCGGCAGGA 
     7081 GCAAGGTGAG ATGACAGGAG ATCCTGCCCC GGCACTTCGC CCAATAGCAG CCAGTCCCTT 
     7141 CCCGCTTCAG TGACAACGTC GAGCACAGCT GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC 
     7201 GATAGCCGCG CTGCCTCGTC TTGCAGTTCA TTCAGGGCAC CGGACAGGTC GGTCTTGACA 
     7261 AAAAGAACCG GGCGCCCCTG CGCTGACAGC CGGAACACGG CGGCATCAGA GCAGCCGATT 
     7321 GTCTGTTGTG CCCAGTCATA GCCGAATAGC CTCTCCACCC AAGCGGCCGG AGAACCTGCG 
     7381 TGCAATCCAT CTTGTTCAAT CATGCGAAAC GATCCTCATC CTGTCTCTTG ATCGATCTTT 
     7441 GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC TCACTACTTC TGGAATAGCT CAGAGGCCGA 
     7501 GGCGGCCTCG GCCTCTGCAT AAATAAAAAA AATTAGTCAG CCATGGGGCG GAGAATGGGC 
     7561 GGAACTGGGC GGAGTTAGGG GCGGGATGGG CGGAGTTAGG GGCGGGACTA TGGTTGCTGA 
     7621 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     7681 CCTGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     7741 GGACTTTCCA CACCCTAACT GACACACATT CCACAGCTGG TTCTTTCCGC CTCAGGACTC 
     7801 TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA 
     7861 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG 
     7921 CCACCTGACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC 
     7981 GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT 
     8041 CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC 
     8101 CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT 
     8161 AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT 
     8221 AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT 
     8281 GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA 
     8341 AAATTTAACG CGAATTTT
//