LOCUS       RC229409                8166 bp    DNA     circular  SYN 
DEFINITION  PLCL1 (Myc-DDK-tagged)-Human phospholipase C-like 1 (PLCL1)
ACCESSION   NM_006226
VERSION     NM_006226.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4323..4414 
                     /label="Myc-DDK"  
     misc_feature    4444..5029 
                     /label="PolyA signal"  
     misc_feature    4501..4520 
                     /label="Restriction Enzyme"  
     misc_feature    5305..5977 
                     /label="ColE1"  
     misc_feature    6407..7201 
                     /label="Kan/Neo"  
     misc_feature    7236..7538 
                     /label="SV40 ori"  
     misc_feature    7624..8101 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4305..4311 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4305 
                     /label="PLCL1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCCGAGGGC GCGGCCGGCA GGGAGGATCC GGCGCCGCCC GACGCGGCGG 
     1081 GGGGCGAAGA CGACCCCCGA GTGGGCCCGG ATGCCGCCGG GGACTGCGTG ACGGCGGCCT 
     1141 CTGGGGGCCG GATGAGGGAC CGTCGCAGCG GGGTCGCACT GCCAGGCGCC GCGGGGACCC 
     1201 CAGCGGACAG CGAGGCGGGC CTCCTGGAGG CAGCACGGGC GACCCCCCGG CGCAGCAGCA 
     1261 TCATCAAGGA TCCTTCAAAC CAAAAATGTG GTGGAAGAAA GAAAACCGTG TCTTTCAGCA 
     1321 GCATGCCATC GGAAAAGAAA ATTAGCAGTG CAAATGACTG CATCAGCTTC ATGCAAGCTG 
     1381 GCTGTGAGTT GAAGAAAGTC CGGCCAAATT CTCGCATTTA CAACCGTTTT TTCACTCTGG 
     1441 ACACAGACCT TCAAGCTCTT CGCTGGGAAC CTTCAAAGAA AGACCTCGAG AAAGCCAAGC 
     1501 TTGATATTTC TGCCATAAAA GAGATCAGAC TGGGGAAAAA CACGGAAACA TTTAGAAACA 
     1561 ATGGCCTTGC TGACCAGATC TGTGAGGACT GTGCCTTTTC CATACTCCAC GGGGAAAACT 
     1621 ATGAGTCTCT GGACCTAGTT GCCAATTCAG CAGATGTGGC AAACATCTGG GTGTCTGGGT 
     1681 TACGGTACCT GGTTTCTCGA AGTAAGCAGC CTCTTGATTT TATGGAGGGC AACCAGAACA 
     1741 CACCACGGTT CATGTGGTTG AAAACAGTGT TTGAAGCAGC AGATGTTGAT GGGAATGGGA 
     1801 TTATGTTGGA AGACACCTCT GTAGAGTTAA TAAAACAACT CAACCCTACT CTGAAGGAAG 
     1861 CCAAGATCAG GTTAAAGTTT AAAGAAATCC AGAAGAGCAA GGAAAAACTA ACCACCCGCG 
     1921 TGACCGAAGA GGAATTTTGT GAAGCTTTTT GTGAACTTTG CACCAGGCCA GAAGTGTATT 
     1981 TCTTACTTGT ACAGATATCT AAAAACAAAG AATATTTGGA TGCCAATGAT CTCATGCTCT 
     2041 TTTTAGAAGC TGAGCAAGGA GTCACCCATA TCACCGAGGA TATATGCTTA GACATCATAA 
     2101 GGAGATACGA ACTTTCTGAA GAGGGACGTC AAAAAGGGTT TCTTGCAATT GATGGCTTTA 
     2161 CCCAGTATTT ATTGTCATCA GAATGTGACA TTTTTGATCC TGAGCAAAAG AAGGTTGCCC 
     2221 AAGATATGAC CCAGCCATTA TCTCACTACT ATATCAATGC CTCTCATAAC ACCTATCTAA 
     2281 TAGAAGACCA GTTCAGGGGG CCAGCTGACA TCAATGGGTA CATTAGAGCT TTGAAAATGG 
     2341 GCTGTCGAAG CGTTGAACTC GATGTAAGTG ATGGTTCAGA TAATGAACCA ATCCTTTGTA 
     2401 ATCGAAATAA CATGACAACC CATGTTTCCT TTCGAAGTGT CATAGAGGTA ATAAATAAAT 
     2461 TTGCCTTTGT TGCTTCTGAA TACCCACTCA TTCTTTGCTT GGGAAATCAC TGCTCCTTGC 
     2521 CGCAGCAGAA GGTAATGGCT CAACAGATGA AAAAGGTCTT TGGCAATAAA CTCTATACTG 
     2581 AAGCACCTTT GCCCTCAGAA TCCTACCTCC CATCACCAGA AAAATTAAAA AGAATGATCA 
     2641 TTGTGAAAGG AAAGAAGTTG CCTTCTGATC CAGATGTGTT AGAAGGAGAA GTAACAGATG 
     2701 AAGATGAAGA AGCTGAAATG TCTCGAAGGA TGTCGGTAGA TTACAATGGT GAGCAGAAGC 
     2761 AAATCCGACT CTGTAGGGAG CTCTCTGATT TGGTGTCTAT TTGTAAATCT GTTCAATACA 
     2821 GGGATTTTGA ACTATCTATG AAAAGCCAAA ACTATTGGGA AATGTGTTCA TTTAGTGAAA 
     2881 CAGAGGCCAG CCGCATTGCA AATGAGTACC CAGAGGATTT TGTTAATTAT AATAAGAAGT 
     2941 TCTTATCAAG AATCTATCCA AGTGCCATGA GGATCGATTC CAGTAACTTG AATCCACAGG 
     3001 ACTTTTGGAA TTGTGGCTGT CAGATTGTAG CAATGAATTT TCAGACTCCG GGTCCAATGA 
     3061 TGGACCTTCA CACGGGCTGG TTTCTTCAAA ACGGGGGATG TGGTTATGTT CTAAGGCCGT 
     3121 CTATAATGCG AGATGAAGTT TCTTACTTCA GCGCAAATAC AAAGGGCATT CTACCTGGGG 
     3181 TGTCTCCTCT AGCTCTTCAT ATCAAGATCA TCAGTGGTCA GAATTTCCCA AAGCCCAAGG 
     3241 GAGCTTGTGC CAAAGGGGAT GTCATAGATC CCTATGTTTG TATAGAGATA CACGGAATTC 
     3301 CAGCGGATTG TTCGGAACAA AGAACTAAAA CTGTACAGCA AAACAGTGAT AATCCTATTT 
     3361 TTGATGAAAC TTTTGAGTTC CAAGTAAACC TACCTGAGCT GGCCATGATC CGTTTTGTTG 
     3421 TTCTGGATGA TGACTACATT GGGGATGAGT TTATAGGGCA ATATACGATA CCATTTGAAT 
     3481 GTTTGCAGCC TGGATATCGG CATGTTCCCC TGCGTTCTTT TGTGGGTGAC ATCATGGAGC 
     3541 ACGTAACCCT TTTTGTCCAC ATAGCAATAA CTAATCGAAG TGGAGGAGGA AAGGCACAGA 
     3601 AGCGCAGTCT TTCAGTGAGA ATGGGGAAGA AAGTTCGGGA ATATACCATG CTCAGGAATA 
     3661 TCGGTCTTAA AACCATTGAT GACATCTTTA AAATAGCGGT TCATCCATTA CGAGAAGCCA 
     3721 TAGATATGAG AGAAAATATG CAGAATGCAA TCGTGTCTAT TAAGGAACTA TGTGGACTCC 
     3781 CTCCAATTGC CAGTCTGAAG CAGTGCCTGT TAACTCTGTC ATCTCGGCTC ATCACCAGTG 
     3841 ACAATACTCC TTCAGTCTCA CTTGTGATGA AAGACAGCTT TCCTTACCTG GAGCCTCTGG 
     3901 GTGCAATTCC AGATGTGCAG AAAAAGATGC TGACTGCTTA TGATCTGATG ATTCAAGAGA 
     3961 GCCGGTTTCT CATAGAAATG GCGGACACAG TCCAGGAAAA GATTGTACAG TGTCAGAAAG 
     4021 CAGGGATGGA GTTCCATGAA GAACTTCATA ATTTGGGGGC AAAAGAAGGC TTGAAGGGAA 
     4081 GAAAACTCAA CAAAGCAACT GAGAGCTTTG CTTGGAACAT TACAGTATTG AAGGGCCAAG 
     4141 GAGATCTGTT GAAGAATGCC AAGAATGAAG CTATAGAAAA CATGAAGCAG ATCCAGCTGG 
     4201 CATGCCTGTC CTGTGGACTG AGTAAAGCCC CCAGCAGCAG TGCTGAGGCC AAGAGCAAGC 
     4261 GCAGCCTGGA AGCCATAGAG GAGAAGGAAA GTAGTGAGGA GAATGGGAAG CTGACGCGTA 
     4321 CGCGGCCGCT CGAGCAGAAA CTCATCTCAG AAGAGGATCT GGCAGCAAAT GATATCCTGG 
     4381 ATTACAAGGA TGACGACGAT AAGGTTTAAA CGGCCGGCCG CGGTCATAGC TGTTTCCTGA 
     4441 ACAGATCCCG GGTGGCATCC CTGTGACCCC TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT 
     4501 GCCACTCCAG TGCCCACCAG CCTTGTCCTA ATAAAATTAA GTTGCATCAT TTTGTCTGAC 
     4561 TAGGTGTCCT TCTATAATAT TATGGGGTGG AGGGGGGTGG TATGGAGCAA GGGGCAAGTT 
     4621 GGGAAGACAA CCTGTAGGGC CTGCGGGGTC TATTGGGAAC CAAGCTGGAG TGCAGTGGCA 
     4681 CAATCTTGGC TCACTGCAAT CTCCGCCTCC TGGGTTCAAG CGATTCTCCT GCCTCAGCCT 
     4741 CCCGAGTTGT TGGGATTCCA GGCATGCATG ACCAGGCTCA GCTAATTTTT GTTTTTTTGG 
     4801 TAGAGACGGG GTTTCACCAT ATTGGCCAGG CTGGTCTCCA ACTCCTAATC TCAGGTGATC 
     4861 TACCCACCTT GGCCTCCCAA ATTGCTGGGA TTACAGGCGT GAACCACTGC TCCCTTCCCT 
     4921 GTCCTTCTGA TTTTAAAATA ACTATACCAG CAGGAGGACG TCCAGACACA GCATAGGCTA 
     4981 CCTGGCCATG CCCAACCGGT GGGACATTTG AGTTGCTTGC TTGGCACTGT CCTCTCATGC 
     5041 GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA TTGGGTACGC GGCCAGCGGC GAGCGGTATC 
     5101 AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA 
     5161 CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT 
     5221 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG 
     5281 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG 
     5341 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG 
     5401 CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC 
     5461 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA 
     5521 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG 
     5581 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC 
     5641 TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC 
     5701 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG 
     5761 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT 
     5821 GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT 
     5881 CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA 
     5941 ATCAATCTAA AGTATATATG AGTAACCTGA GGCTATGGCA GGGCCTGCCG CCCCGACGTT 
     6001 GGCTGCGAGC CCTGGGCCTT CACCCGAACT TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC 
     6061 GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG TGGGGTATCG ACAGAGTGCC AGCCCTGGGA 
     6121 CCGAACCCCG CGTTTATGAA CAAACGACCC AACACCGTGC GTTTTATTCT GTCTTTTTAT 
     6181 TGCCGTCATA GCGCGGGTTC CTTCCGGTAT TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC 
     6241 CCTAGGGTGG GCGAAGAACT CCAGCATGAG ATCCCCGCGC TGGAGGATCA TCCAGCCGGC 
     6301 GTCCCGGAAA ACGATTCCGA AGCCCAACCT TTCATAGAAG GCGGCGGTGG AATCGAAATC 
     6361 TCGTGATGGC AGGTTGGGCG TCGCTTGGTC GGTCATTTCG AACCCCAGAG TCCCGCTCAG 
     6421 AAGAACTCGT CAAGAAGGCG ATAGAAGGCG ATGCGCTGCG AATCGGGAGC GGCGATACCG 
     6481 TAAAGCACGA GGAAGCGGTC AGCCCATTCG CCGCCAAGCT CTTCAGCAAT ATCACGGGTA 
     6541 GCCAACGCTA TGTCCTGATA GCGATCCGCC ACACCCAGCC GGCCACAGTC GATGAATCCA 
     6601 GAAAAGCGGC CATTTTCCAC CATGATATTC GGCAAGCAGG CATCGCCATG GGTCACGACG 
     6661 AGATCCTCGC CGTCGGGCAT GCTCGCCTTG AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC 
     6721 CCCTGATGCT CTTCGTCCAG ATCATCCTGA TCGACAAGAC CGGCTTCCAT CCGAGTACGT 
     6781 GCTCGCTCGA TGCGATGTTT CGCTTGGTGG TCGAATGGGC AGGTAGCCGG ATCAAGCGTA 
     6841 TGCAGCCGCC GCATTGCATC AGCCATGATG GATACTTTCT CGGCAGGAGC AAGGTGAGAT 
     6901 GACAGGAGAT CCTGCCCCGG CACTTCGCCC AATAGCAGCC AGTCCCTTCC CGCTTCAGTG 
     6961 ACAACGTCGA GCACAGCTGC GCAAGGAACG CCCGTCGTGG CCAGCCACGA TAGCCGCGCT 
     7021 GCCTCGTCTT GCAGTTCATT CAGGGCACCG GACAGGTCGG TCTTGACAAA AAGAACCGGG 
     7081 CGCCCCTGCG CTGACAGCCG GAACACGGCG GCATCAGAGC AGCCGATTGT CTGTTGTGCC 
     7141 CAGTCATAGC CGAATAGCCT CTCCACCCAA GCGGCCGGAG AACCTGCGTG CAATCCATCT 
     7201 TGTTCAATCA TGCGAAACGA TCCTCATCCT GTCTCTTGAT CGATCTTTGC AAAAGCCTAG 
     7261 GCCTCCAAAA AAGCCTCCTC ACTACTTCTG GAATAGCTCA GAGGCCGAGG CGGCCTCGGC 
     7321 CTCTGCATAA ATAAAAAAAA TTAGTCAGCC ATGGGGCGGA GAATGGGCGG AACTGGGCGG 
     7381 AGTTAGGGGC GGGATGGGCG GAGTTAGGGG CGGGACTATG GTTGCTGACT AATTGAGATG 
     7441 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC TGGTTGCTGA 
     7501 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     7561 CCCTAACTGA CACACATTCC ACAGCTGGTT CTTTCCGCCT CAGGACTCTT CCTTTTTCAA 
     7621 TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT 
     7681 TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGCG 
     7741 CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA 
     7801 CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC 
     7861 GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT 
     7921 TTACGGCACC TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG 
     7981 CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC 
     8041 TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG 
     8101 ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG 
     8161 AATTTT
//