LOCUS       RC229400L1                9333 bp    DNA     circular  SYN 
DEFINITION  ANO1 (Myc-DDK-tagged)-Human anoctamin 1, calcium activated chloride channel (ANO1), transcript variant 1
ACCESSION   NM_018043
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    7624..7804 
                     /label="3'LTR"  
     misc_feature    833..1013 
                     /label="5'LTR"  
     misc_feature    8532..9191 
                     /label="CAT(Cam-r)"  
     misc_feature    2620..3234 
                     /label="CMV promoter"  
     misc_feature    7963..8531 
                     /label="ColE1"  
     misc_feature    6499..6519 
                     /label="LR50 primer"  
     misc_feature    6296..6367 
                     /label="Myc-DDK"  
     misc_feature    3252..3271 
                     /label="T7 promoter"  
     misc_feature    3221..3237 
                     /label="V2 primer"  
     misc_feature    6508..7099 
                     /label="WPRE"  
     misc_feature    3320..3328 
                     /label="Restriction Enzyme"  
     misc_feature    6278..6284 
                     /label="Restriction Enzyme"  
     misc_feature    3320..6278 
                     /label="ANO1"  
ORIGIN                      
        1 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG 
       61 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT 
      121 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC 
      181 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT CGCGTTGACA 
      241 TTGATTATTG ACTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA 
      301 TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA 
      361 CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT 
      421 CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT 
      481 GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA 
      541 TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT 
      601 CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC AATGGGCGTG GATAGCGGTT 
      661 TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT TGTTTTGGCA 
      721 CCAAAATCAA CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA CGCAAATGGG 
      781 CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGCGCG TTTTGCCTGT ACTGGGTCTC 
      841 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 
      901 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 
      961 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 
     1021 CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG 
     1081 GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT 
     1141 TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG 
     1201 GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA 
     1261 ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT 
     1321 GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC 
     1381 AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA 
     1441 AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA 
     1501 AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT 
     1561 ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA 
     1621 GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA 
     1681 ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA 
     1741 ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT 
     1801 TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG 
     1861 CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG 
     1921 ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG 
     1981 AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA 
     2041 ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA 
     2101 AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC 
     2161 ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT 
     2221 TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA 
     2281 TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA 
     2341 GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCGGCACTG 
     2401 CGTGCGCCAA TTCTGCAGAC AAATGGCAGT ATTCATCCAC AATTTTAAAA GAAAAGGGGG 
     2461 GATTGGGGGG TACAGTGCAG GGGAAAGAAT AGTAGACATA ATAGCAACAG ACATACAAAC 
     2521 TAAAGAATTA CAAAAACAAA TTACAAAAAT TCAAAATTTT CGGGTTTATT ACAGGGACAG 
     2581 CAGAGATCCA GTTTGGTTAG TACCGGGCCC GCTCTAGACA TGTCCAATAT GACCGCCATG 
     2641 TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG 
     2701 CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC 
     2761 CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG 
     2821 GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA 
     2881 TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC 
     2941 CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT 
     3001 ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA 
     3061 GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT 
     3121 TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA 
     3181 AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG 
     3241 TCAGAATTTT GTAATACGAC TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT 
     3301 ACCGAGGAGA TCTGCCGCCG CGATCGCCAT GAGGGTCAAC GAGAAGTACT CGACGCTCCC 
     3361 GGCCGAGGAC CGCAGCGTCC ACATCATCAA CATCTGCGCC ATCGAGGACA TCGGCTACCT 
     3421 GCCGTCCGAG GGCACGCTGC TGAACTCCTT ATCTGTGGAC CCTGATGCCG AGTGCAAGTA 
     3481 TGGCCTGTAC TTCAGGGACG GCCGGCGCAA GGTGGACTAC ATCCTGGTGT ACCATCACAA 
     3541 GAGGCCCTCG GGCAACCGGA CCCTGGTCAG GAGGGTGCAG CACAGCGACA CCCCCTCTGG 
     3601 GGCTCGCAGC GTCAAGCAGG ACCACCCCCT GCCGGGCAAG GGGGCGTCGC TGGATGCAGG 
     3661 CTCGGGGGAG CCCCCGATGG ACTACCACGA GGATGACAAG CGCTTCCGCA GGGAGGAGTA 
     3721 CGAGGGCAAC CTCCTGGAGG CGGGCCTGGA GCTGGAGCGG GACGAGGACA CTAAAATCCA 
     3781 CGGAGTCGGG TTTGTGAAAA TCCATGCCCC CTGGAACGTG CTGTGCAGAG AGGCCGAGTT 
     3841 TCTGAAACTG AAGATGCCGA CGAAGAAGAT GTACCACATT AATGAGACCC GTGGCCTCCT 
     3901 GAAAAAAATC AACTCTGTGC TCCAGAAAAT CACAGATCCC ATCCAGCCCA AAGTGGCTGA 
     3961 GCACAGGCCC CAGACCATGA AGAGACTCTC CTATCCCTTC TCCCGGGAGA AGCAGCATCT 
     4021 ATTTGACTTG TCTGATAAGG ATTCCTTTTT CGACAGCAAA ACCCGGAGCA CGATTGTCTA 
     4081 TGAGATCTTG AAGAGAACGA CGTGTACAAA GGCCAAGTAC AGCATGGGCA TCACGAGCCT 
     4141 GCTGGCCAAT GGTGTGTACG CGGCTGCATA CCCACTGCAC GATGGAGACT ACAACGGTGA 
     4201 AAACGTCGAG TTCAACGACA GAAAACTCCT GTACGAAGAG TGGGCACGCT ATGGAGTTTT 
     4261 CTATAAGTAC CAGCCCATCG ACCTGGTCAG GAAGTATTTT GGGGAGAAGA TCGGCCTGTA 
     4321 CTTCGCCTGG CTGGGCGTGT ACACCCAGAT GCTCATCCCT GCCTCCATCG TGGGAATCAT 
     4381 TGTCTTCCTG TACGGATGCG CCACCATGGA TGAAAACATC CCCAGCATGG AGATGTGTGA 
     4441 CCAGAGACAC AATATCACCA TGTGCCCGCT TTGCGACAAG ACCTGCAGCT ACTGGAAGAT 
     4501 GAGCTCAGCC TGCGCCACGG CCCGCGCCAG CCACCTCTTC GACAACCCCG CCACGGTCTT 
     4561 CTTCTCTGTC TTCATGGCCC TCTGGGCTGC CACCTTCATG GAGCACTGGA AGCGGAAACA 
     4621 GATGCGACTC AACTACCGCT GGGACCTCAC GGGCTTTGAA GAGGAAGAGG AGGCTGTCAA 
     4681 GGATCATCCT AGAGCTGAAT ACGAAGCCAG AGTCTTGGAG AAGTCTCTGA AGAAAGAGTC 
     4741 CAGAAACAAA GAGAAGCGCC GGCATATTCC AGAGGAGTCA ACAAACAAAT GGAAGCAGAG 
     4801 GGTTAAGACA GCCATGGCGG GGGTGAAATT GACTGACAAA GTGAAGCTGA CATGGAGAGA 
     4861 TCGGTTCCCA GCCTACCTCA CTAACTTGGT CTCCATCATC TTCATGATTG CAGTGACGTT 
     4921 TGCCATCGTC CTCGGCGTCA TCATCTACAG AATCTCCATG GCCGCCGCCT TGGCCATGAA 
     4981 CTCCTCCCCC TCCGTGCGGT CCAACATCCG GGTCACAGTC ACAGCCACCG CAGTCATCAT 
     5041 CAACCTAGTG GTCATCATCC TCCTGGACGA GGTGTATGGC TGCATAGCCC GATGGCTCAC 
     5101 CAAGATCGAG GTCCCAAAGA CGGAGAAAAG CTTTGAGGAG AGGCTGATCT TCAAGGCTTT 
     5161 CCTGCTGAAG TTTGTGAATT CCTACACCCC CATCTTTTAC GTGGCGTTCT TCAAAGGCCG 
     5221 GTTTGTTGGA CGCCCGGGCG ACTACGTGTA CATTTTCCGT TCCTTCCGAA TGGAAGAGTG 
     5281 TGCGCCAGGG GGCTGCCTGA TGGAGCTATG CATCCAGCTC AGCATCATCA TGCTGGGGAA 
     5341 ACAGCTGATC CAGAACAACC TGTTCGAGAT CGGCATCCCG AAGATGAAGA AGCTCATCCG 
     5401 CTACCTGAAG CTGAAGCAGC AGAGCCCCCC TGACCACGAG GAGTGTGTGA AGAGGAAACA 
     5461 GCGGTACGAG GTGGATTACA ACCTGGAGCC CTTCGCGGGC CTCACCCCAG AGTACATGGA 
     5521 AATGATCATC CAGTTTGGCT TCGTCACCCT GTTTGTCGCC TCCTTCCCCC TGGCCCCACT 
     5581 GTTTGCGCTG CTGAACAACA TCATCGAGAT CCGCCTGGAC GCCAAAAAGT TTGTCACTGA 
     5641 GCTCCGAAGG CCGGTAGCTG TCAGAGCCAA AGACATCGGA ATCTGGTACA ATATCCTCAG 
     5701 AGGCATTGGG AAGCTTGCTG TCATCATCAA TGCCTTCGTG ATCTCCTTCA CGTCTGACTT 
     5761 CATCCCGCGC CTGGTGTACC TCTACATGTA CAGTAAGAAC GGGACCATGC ACGGCTTCGT 
     5821 CAACCACACC CTCTCCTCCT TCAACGTCAG TGACTTCCAG AACGGCACGG CCCCCAATGA 
     5881 CCCCCTGGAC CTGGGCTACG AGGTGCAGAT CTGCAGGTAT AAAGACTACC GAGAGCCGCC 
     5941 GTGGTCGGAA AACAAGTACG ACATCTCCAA GGACTTCTGG GCCGTCCTGG CAGCCCGGCT 
     6001 GGCGTTTGTC ATCGTCTTCC AGAACCTGGT CATGTTCATG AGCGACTTTG TGGACTGGGT 
     6061 CATCCCGGAC ATCCCCAAGG ACATCAGCCA GCAGATCCAC AAGGAGAAGG TGCTCATGGT 
     6121 GGAGCTGTTC ATGCGGGAGG AGCAAGACAA GCAGCAGCTG CTGGAAACCT GGATGGAGAA 
     6181 GGAGCGGCAG AAGGACGAGC CGCCGTGCAA CCACCACAAC ACCAAAGCCT GCCCAGACAG 
     6241 CCTCGGCAGC CCAGCCCCCA GCCATGCCTA CCACGGGGGC GTCCTGACGC GTACGCGGCC 
     6301 GCTCGAGCAG AAACTCATCT CAGAAGAGGA TCTGGCAGCA AATGATATCC TGGATTACAA 
     6361 GGATGACGAC GATAAGGTTT AAACGGCCGG CCGCGGTCTG TACAAGTAGG ATTCGTCGAG 
     6421 GGACCTAATA ACTTCGTATA GCATACATTA TACGAAGTTA TACATGTTTA AGGGTTCCGG 
     6481 TTCCACTAGG TACAATTCGA TATCAAGCTT ATCGATAATC AACCTCTGGA TTACAAAATT 
     6541 TGTGAAAGAT TGACTGGTAT TCTTAACTAT GTTGCTCCTT TTACGCTATG TGGATACGCT 
     6601 GCTTTAATGC CTTTGTATCA TGCTATTGCT TCCCGTATGG CTTTCATTTT CTCCTCCTTG 
     6661 TATAAATCCT GGTTGCTGTC TCTTTATGAG GAGTTGTGGC CCGTTGTCAG GCAACGTGGC 
     6721 GTGGTGTGCA CTGTGTTTGC TGACGCAACC CCCACTGGTT GGGGCATTGC CACCACCTGT 
     6781 CAGCTCCTTT CCGGGACTTT CGCTTTCCCC CTCCCTATTG CCACGGCGGA ACTCATCGCC 
     6841 GCCTGCCTTG CCCGCTGCTG GACAGGGGCT CGGCTGTTGG GCACTGACAA TTCCGTGGTG 
     6901 TTGTCGGGGA AATCATCGTC CTTTCCTTGG CTGCTCGCCT GTGTTGCCAC CTGGATTCTG 
     6961 CGCGGGACGT CCTTCTGCTA CGTCCCTTCG GCCCTCAATC CAGCGGACCT TCCTTCCCGC 
     7021 GGCCTGCTGC CGGCTCTGCG GCCTCTTCCG CGTCTTCGCC TTCGCCCTCA GACGAGTCGG 
     7081 ATCTCCCTTT GGGCCGCCTC CCCGCATCGA TACCGTCGAC CTCGATCGAG ACCTAGAAAA 
     7141 ACATGGAGCA ATCACAAGTA GCAATACAGC AGCTACCAAT GCTGATTGTG CCTGGCTAGA 
     7201 AGCACAAGAG GAGGAGGAGG TGGGTTTTCC AGTCACACCT CAGGTACCTT TAAGACCAAT 
     7261 GACTTACAAG GCAGCTGTAG ATCTTAGCCA CTTTTTAAAA GAAAAGGGGG GACTGGAAGG 
     7321 GCTAATTCAC TCCCAACGAA GACAAGATAT CCTTGATCTG TGGATCTACC ACACACAAGG 
     7381 CTACTTCCCT GATTGGCAGA ACTACACACC AGGGCCAGGG ATCAGATATC CACTGACCTT 
     7441 TGGATGGTGC TACAAGCTAG TACCAGTTGA GCAAGAGAAG GTAGAAGAAG CCAATGAAGG 
     7501 AGAGAACACC CGCTTGTTAC ACCCTGTGAG CCTGCATGGG ATGGATGACC CGGAGAGAGA 
     7561 AGTATTAGAG TGGAGGTTTG ACAGCCGCCT AGCATTTCAT CACATGGCCC GAGAGCTGCA 
     7621 TCCGGACTGT ACTGGGTCTC TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA 
     7681 ACTAGGGAAC CCACTGCTTA AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG 
     7741 TGCCCGTCTG TTGTGTGACT CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG 
     7801 GAAAATCTCT AGCAGCATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC 
     7861 CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG 
     7921 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG 
     7981 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT 
     8041 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT 
     8101 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG 
     8161 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT 
     8221 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT 
     8281 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT 
     8341 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC 
     8401 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC 
     8461 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG 
     8521 TTAAGGGATT TTGGTCATGA TTACGCCCCG CCCTGCCACT CATCGCAGTA CTGTTGTAAT 
     8581 TCATTAAGCA TTCTGCCGAC ATGGAAGCCA TCACAAACGG CATGATGAAC CTGAATCGCC 
     8641 AGCGGCATCA GCACCTTGTC GCCTTGCGTA TAATATTTGC CCATGGTGAA AACGGGGGCG 
     8701 AAGAAGTTGT CCATATTGGC CACGTTTAAA TCAAAACTGG TGAAACTCAC CCAGGGATTG 
     8761 GCTGAGACGA AAAACATATT CTCAATAAAC CCTTTAGGGA AATAGGCCAG GTTTTCACCG 
     8821 TAACACGCCA CATCTTGCGA ATATATGTGT AGAAACTGCC GGAAATCGTC GTGGTATTCA 
     8881 CTCCAGAGCG ATGAAAACGT TTCAGTTTGC TCATGGAAAA CGGTGTAACA AGGGTGAACA 
     8941 CTATCCCATA TCACCAGCTC ACCGTCTTTC ATTGCCATAC GGAACTCCGG ATGAGCATTC 
     9001 ATCAGGCGGG CAAGAATGTG AATAAAGGCC GGATAAAACT TGTGCTTATT TTTCTTTACG 
     9061 GTCTTTAAAA AGGCCGTAAT ATCCAGCTGA ACGGTCTGGT TATAGGTACA TTGAGCAACT 
     9121 GACTGAAATG CCTCAAAATG TTCTTTACGA TGCCATTGGG ATATATCAAC GGTGGTATAT 
     9181 CCAGTGATTT TTTTCTCCAT ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT 
     9241 TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTC 
     9301 CCGCGCACAT TTCCCCGAAA AGTGCCACCT GAC
//