LOCUS       RC229397                7734 bp    DNA     circular  SYN 
DEFINITION  PCDH20 (Myc-DDK-tagged)-Human protocadherin 20 (PCDH20)
ACCESSION   NM_022843
VERSION     NM_022843.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3891..3982 
                     /label="Myc-DDK"  
     misc_feature    4012..4597 
                     /label="PolyA signal"  
     misc_feature    4069..4088 
                     /label="Restriction Enzyme"  
     misc_feature    4873..5545 
                     /label="ColE1"  
     misc_feature    5975..6769 
                     /label="Kan/Neo"  
     misc_feature    6804..7106 
                     /label="SV40 ori"  
     misc_feature    7192..7669 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3873..3879 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3873 
                     /label="PCDH20"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCGCGGCCGA GGGAATGCGC GCAGCTCACA GGCCCTGGGA GTGAGCTGGT 
     1081 GCCCGGCGAC CTGGCACCCG CGCCTGGATA TGGGGCGTCT ACATCGTCCC AGGAGCAGCA 
     1141 CCAGCTACAG GAACCTGCCG CATCTGTTTC TGTTTTTCCT CTTCGTGGGA CCCTTCAGCT 
     1201 GCCTCGGGAG TTACAGCCGG GCCACCGAGC TTCTGTACAG CCTAAACGAG GGACTACCCG 
     1261 CGGGGGTGCT CATCGGCAGC CTGGCCGAGG ACCTGCGGCT GCTGCCCAGG TCTGCAGGGA 
     1321 GGCCGGACCC GCAGTCGCAG CTGCCAGAGC GCACCGGTGC TGAGTGGAAC CCCCCTCTCT 
     1381 CCTTCAGCCT GGCCTCCCGG GGACTGAGTG GCCAGTACGT GACCCTAGAC AACCGCTCTG 
     1441 GGGAGCTGCA CACTTCAGCT CAGGAGATCG ACAGGGAGGC CCTGTGTGTT GAAGGGGGTG 
     1501 GAGGGACTGC GTGGAGCGGC AGCGTTTCCA TCTCCTCCTC TCCTTCTGAC TCTTGTCTTT 
     1561 TGCTGCTGGA TGTGCTTGTC CTGCCTCAGG AATACTTCAG GTTTGTGAAG GTGAAGATCG 
     1621 CCATCAGAGA CATCAATGAC AACGCCCCGC AGTTCCCTGT TTCCCAGATC TCGGTGTGGG 
     1681 TCCCGGAAAA TGCACCTGTA AACACCCGAC TGGCCATAGA GCATCCTGCT GTGGACCCAG 
     1741 ATGTAGGCAT TAATGGGGTA CAGACCTATC GCTTACTGGA CTACCATGGT ATGTTCACCC 
     1801 TGGACGTGGA GGAGAATGAG AATGGGGAGC GCACCCCCTA CCTAATTGTC ATGGGTGCTT 
     1861 TGGACAGGGA AACCCAGGAC CAGTATGTGA GCATCATCAT AGCTGAGGAT GGTGGGTCTC 
     1921 CACCACTTTT GGGCAGTGCC ACTCTCACCA TTGGCATCAG TGACATTAAT GACAATTGCC 
     1981 CTCTCTTCAC AGACTCACAA ATCAATGTCA CTGTGTATGG GAATGCTACA GTGGGCACCC 
     2041 CAATTGCAGC TGTCCAGGCT GTGGATAAAG ACTTGGGGAC CAATGCTCAA ATTACTTATT 
     2101 CTTACAGTCA GAAAGTTCCA CAAGCATCTA AGGATTTATT TCACCTGGAT GAAAACACTG 
     2161 GAGTCATTAA ACTTTTCAGT AAGATTGGAG GAAGTGTTCT GGAGTCCCAC AAGCTCACCA 
     2221 TCCTTGCTAA TGGACCAGGC TGCATCCCTG CTGTAATCAC TGCTCTTGTG TCCATTATTA 
     2281 AAGTTATTTT CAGACCCCCT GAAATTGTCC CTCGTTACAT AGCAAACGAG ATAGATGGTG 
     2341 TGGTTTATCT GAAAGAACTG GAACCCGTTA ACACTCCCAT TGCGTTTTTC ACCATAAGAG 
     2401 ATCCAGAAGG TAAATACAAG GTTAACTGCT ACCTGGATGG TGAAGGGCCG TTTAGGTTAT 
     2461 CACCTTACAA ACCATACAAT AATGAATATT TACTAGAGAC CACAAAACCT ATGGACTATG 
     2521 AGCTACAGCA GTTCTATGAA GTAGCTGTGG TGGCTTGGAA CTCTGAGGGA TTTCATGTCA 
     2581 AAAGGGTCAT TAAAGTGCAA CTTTTAGATG ACAATGATAA TGCTCCAATT TTCCTTCAAC 
     2641 CCTTAATAGA ACTAACCATC GAAGAGAACA ACTCACCCAA TGCCTTTTTG ACTAAGCTGT 
     2701 ATGCTACAGA TGCCGACAGC GAGGAGAGAG GCCAAGTTTC ATATTTTCTG GGACCTGATG 
     2761 CTCCATCATA TTTTTCCTTA GACAGTGTCA CAGGAATTCT GACAGTTTCT ACTCAGCTGG 
     2821 ACCGAGAAGA GAAAGAAAAG TACAGATACA CTGTCAGAGC TGTTGACTGT GGGAAGCCAC 
     2881 CCAGAGAATC AGTAGCCACT GTGGCCCTCA CAGTGTTGGA TAAAAATGAC AACAGTCCTC 
     2941 GGTTTATCAA CAAGGACTTC AGCTTTTTTG TGCCTGAAAA CTTTCCAGGC TATGGTGAGA 
     3001 TTGGAGTAAT TAGTGTAACA GATGCTGACG CTGGACGAAA TGGATGGGTC GCCCTCTCTG 
     3061 TGGTGAACCA GAGTGATATT TTTGTCATAG ATACAGGAAA GGGTATGCTG AGGGCTAAAG 
     3121 TCTCTTTGGA CAGAGAGCAG CAAAGCTCCT ATACTTTGTG GGTTGAAGCT GTTGATGGGG 
     3181 GTGAGCCTGC CCTCTCCTCT ACAGCAAAAA TCACAATTCT CCTTCTAGAT ATCAATGACA 
     3241 ACCCTCCTCT TGTTTTGTTT CCTCAGTCTA ATATGTCTTA TCTGTTAGTA CTGCCTTCTA 
     3301 CTCTGCCAGG CTCCCCGGTT ACAGAAGTCT ATGCTGTCGA CAAAGACACA GGCATGAATG 
     3361 CTGTCATAGC TTACAGCATC ATAGGGAGAA GAGGTCCTAG GCCTGAGTCC TTCAGGATTG 
     3421 ACCCTAAAAC TGGCAACATT ACTTTGGAAG AGGCATTGCT GCAGACAGAT TATGGGCTCC 
     3481 ATCGCTTACT GGTGAAAGTG AGTGATCATG GTTATCCCGA GCCTCTCCAC TCCACAGTCA 
     3541 TGGTGAACCT ATTTGTCAAT GACACTGTCA GTAATGAGAG TTACATTGAG AGTCTTTTAA 
     3601 GAAAAGAACC AGAGATTAAT ATAGAGGAGA AAGAACCACA AATCTCAATA GAACCGACTC 
     3661 ATAGGAAGGT AGAATCTGTG TCTTGTATGC CCACCTTAGT AGCTCTGTCT GTAATAAGCT 
     3721 TGGGTTCCAT CACACTGGTC ACAGGGATGG GCATATACAT CTGTTTAAGG AAAGGGGAAA 
     3781 AGCATCCCAG GGAAGATGAA AATTTGGAAG TACAGATTCC ACTGAAAGGA AAAATTGACT 
     3841 TGCATATGCG AGAGAGAAAG CCAATGGATA TTTCTAATAT TACGCGTACG CGGCCGCTCG 
     3901 AGCAGAAACT CATCTCAGAA GAGGATCTGG CAGCAAATGA TATCCTGGAT TACAAGGATG 
     3961 ACGACGATAA GGTTTAAACG GCCGGCCGCG GTCATAGCTG TTTCCTGAAC AGATCCCGGG 
     4021 TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG 
     4081 CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC 
     4141 TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC 
     4201 TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC 
     4261 ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG 
     4321 GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT 
     4381 TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG 
     4441 CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT 
     4501 TTAAAATAAC TATACCAGCA GGAGGACGTC CAGACACAGC ATAGGCTACC TGGCCATGCC 
     4561 CAACCGGTGG GACATTTGAG TTGCTTGCTT GGCACTGTCC TCTCATGCGT TGGGTCCACT 
     4621 CAGTAGATGC CTGTTGAATT GGGTACGCGG CCAGCGGCGA GCGGTATCAG CTCACTCAAA 
     4681 GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA 
     4741 AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT 
     4801 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 
     4861 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 
     4921 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 
     4981 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 
     5041 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 
     5101 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 
     5161 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 
     5221 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 
     5281 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 
     5341 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC 
     5401 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC 
     5461 AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG 
     5521 TATATATGAG TAACCTGAGG CTATGGCAGG GCCTGCCGCC CCGACGTTGG CTGCGAGCCC 
     5581 TGGGCCTTCA CCCGAACTTG GGGGGTGGGG TGGGGAAAAG GAAGAAACGC GGGCGTATTG 
     5641 GCCCCAATGG GGTCTCGGTG GGGTATCGAC AGAGTGCCAG CCCTGGGACC GAACCCCGCG 
     5701 TTTATGAACA AACGACCCAA CACCGTGCGT TTTATTCTGT CTTTTTATTG CCGTCATAGC 
     5761 GCGGGTTCCT TCCGGTATTG TCTCCTTCCG TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC 
     5821 GAAGAACTCC AGCATGAGAT CCCCGCGCTG GAGGATCATC CAGCCGGCGT CCCGGAAAAC 
     5881 GATTCCGAAG CCCAACCTTT CATAGAAGGC GGCGGTGGAA TCGAAATCTC GTGATGGCAG 
     5941 GTTGGGCGTC GCTTGGTCGG TCATTTCGAA CCCCAGAGTC CCGCTCAGAA GAACTCGTCA 
     6001 AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA TCGGGAGCGG CGATACCGTA AAGCACGAGG 
     6061 AAGCGGTCAG CCCATTCGCC GCCAAGCTCT TCAGCAATAT CACGGGTAGC CAACGCTATG 
     6121 TCCTGATAGC GATCCGCCAC ACCCAGCCGG CCACAGTCGA TGAATCCAGA AAAGCGGCCA 
     6181 TTTTCCACCA TGATATTCGG CAAGCAGGCA TCGCCATGGG TCACGACGAG ATCCTCGCCG 
     6241 TCGGGCATGC TCGCCTTGAG CCTGGCGAAC AGTTCGGCTG GCGCGAGCCC CTGATGCTCT 
     6301 TCGTCCAGAT CATCCTGATC GACAAGACCG GCTTCCATCC GAGTACGTGC TCGCTCGATG 
     6361 CGATGTTTCG CTTGGTGGTC GAATGGGCAG GTAGCCGGAT CAAGCGTATG CAGCCGCCGC 
     6421 ATTGCATCAG CCATGATGGA TACTTTCTCG GCAGGAGCAA GGTGAGATGA CAGGAGATCC 
     6481 TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG TCCCTTCCCG CTTCAGTGAC AACGTCGAGC 
     6541 ACAGCTGCGC AAGGAACGCC CGTCGTGGCC AGCCACGATA GCCGCGCTGC CTCGTCTTGC 
     6601 AGTTCATTCA GGGCACCGGA CAGGTCGGTC TTGACAAAAA GAACCGGGCG CCCCTGCGCT 
     6661 GACAGCCGGA ACACGGCGGC ATCAGAGCAG CCGATTGTCT GTTGTGCCCA GTCATAGCCG 
     6721 AATAGCCTCT CCACCCAAGC GGCCGGAGAA CCTGCGTGCA ATCCATCTTG TTCAATCATG 
     6781 CGAAACGATC CTCATCCTGT CTCTTGATCG ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA 
     6841 GCCTCCTCAC TACTTCTGGA ATAGCTCAGA GGCCGAGGCG GCCTCGGCCT CTGCATAAAT 
     6901 AAAAAAAATT AGTCAGCCAT GGGGCGGAGA ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG 
     6961 GATGGGCGGA GTTAGGGGCG GGACTATGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     7021 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCTG GTTGCTGACT AATTGAGATG 
     7081 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC CTAACTGACA 
     7141 CACATTCCAC AGCTGGTTCT TTCCGCCTCA GGACTCTTCC TTTTTCAATA TTATTGAAGC 
     7201 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA 
     7261 CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC 
     7321 GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC 
     7381 CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC 
     7441 CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC 
     7501 GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG 
     7561 GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT 
     7621 GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT 
     7681 TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
//