LOCUS       RC229076                9003 bp    DNA     circular  SYN 
DEFINITION  PPP1R9A (Myc-DDK-tagged)-Human protein phosphatase 1, regulatory (inhibitor) subunit 9A (PPP1R9A), transcript variant 1
ACCESSION   NM_001166160
VERSION     NM_001166160.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5160..5251 
                     /label="Myc-DDK"  
     misc_feature    5281..5866 
                     /label="PolyA signal"  
     misc_feature    5338..5357 
                     /label="Restriction Enzyme"  
     misc_feature    6142..6814 
                     /label="ColE1"  
     misc_feature    7244..8038 
                     /label="Kan/Neo"  
     misc_feature    8073..8375 
                     /label="SV40 ori"  
     misc_feature    8461..8938 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5142..5148 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5142 
                     /label="PPP1R9A"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTTGAAAACT GAGTCTTCAG GTGAACGAAC CACTCTCAGA AGTGCCTCTC 
     1081 CTCACAGGAA TGCATATCGA ACTGAGTTTC AGGCACTGAA AAGTACCTTT GACAAACCCA 
     1141 AGTCAGATGG GGAACAAAAA ACAAAAGAAG GTGAGGGCTC CCAGCAGAGC AGGGGGAGGA 
     1201 AATATGGCTC CAATGTCAAC AGAATTAAAA ACCTATTTAT GCAGATGGGT ATGGAACCCA 
     1261 ACGAGAATGC TGCAGTCATT GCCAAAACAA GGGGGAAAGG TGGACATTCA TCTCCTCAGA 
     1321 GAAGAATGAA GCCCAAAGAA TTTCTGGAAA AAACAGATGG CTCAGTTGTT AAGTTGGAGT 
     1381 CTTCTGTTTC TGAACGAATT AGTAGATTTG ACACTATGTA CGATGGCCCT TCATATTCCA 
     1441 AGTTCACTGA GACTCGAAAG ATGTTTGAGA GAAGTGTGCA TGAATCAGGA CAGAACAACC 
     1501 GCTATTCCCC AAAGAAAGAG AAAGCTGGAG GGAGTGAACC TCAGGATGAA TGGGGAGGTT 
     1561 CCAAGTCCAA CAGAGGCAGT ACTGATTCCT TGGACAGCCT TAGCTCCCGA ACTGAGGCTG 
     1621 TCTCCCCAAC TGTGAGTCAA CTGAGTGCAG TATTTGAGAA CACTGATTCT CCCAGTGCCA 
     1681 TCATTTCTGA GAAGGCTGAA AACAATGAAT ACTCAGTGAC TGGGCATTAT CCCTTGAATT 
     1741 TACCATCTGT TACTGTTACA AATCTTGACA CATTTGGTCA CCTGAAGGAT TCTAATTCCT 
     1801 GGCCTCCTTC AAACAAGCGA GGTGTTGATA CAGAGGATGC TCACAAGAGT AATGCAACTC 
     1861 CAGTACCAGA AGTGGCTTCT AAAAGTACCT CTCTAGCTTC GATACCTGGT GAAGAGATCC 
     1921 AGCAGAGCAA GGAACCCGAG GACTCCACAT CTAATCAACA GACTCCCGAC AGCATTGACA 
     1981 AAGATGGTCC TGAAGAACCT TGTGCTGAAA GTAAGGCAAT GCCAAAGTCC GAAATCCCTT 
     2041 CACCACAAAG CCAACTGTTA GAAGATGCTG AAGCTAATTT GGTTGGAAGG GAGGCAGCAA 
     2101 AGCAACAGAG GAAAGAACTT GCAGGTGGTG ATTTCACCTC TCCTGATGCT TCTGCATCCA 
     2161 GTTCTGGAAA AGAAGTACCT GAAGATTCAA ATAATTTTGA TGGTTCCCAT GTGTACATGC 
     2221 ACAGTGACTA TAATGTGTAT AGGGTGAGAT CCAGGTATAA TTCAGACTGG GGAGAGACAG 
     2281 GCACTGAGCA GGATGAGGAG GAAGATAGTG ATGAGAACAG TTACTATCAG CCTGATATGG 
     2341 AGTACTCGGA AATTGTTGGA TTGCCAGAAG AAGAAGAAAT CCCAGCAAAT AGGAAAATTA 
     2401 AGTTTAGTAG TGCTCCTATT AAGGTTTTCA ACACATACTC CAATGAAGAC TATGACAGGA 
     2461 GAAATGACGA AGTTGACCCT GTGGCTGCTT CAGCTGAGTA TGAACTTGAA AAACGTGTAG 
     2521 AAAAGCTGGA ACTTTTCCCA GTGGAGCTAG AGAAAGATGA GGATGGTCTT GGTATAAGTA 
     2581 TTATTGGAAT GGGTGTTGGA GCAGATGCTG GACTTGAAAA GCTGGGAATA TTCGTCAAGA 
     2641 CAGTAACAGA AGGTGGTGCT GCTCAACGGG ATGGCAGAAT ACAAGTCAAT GACCAGATTG 
     2701 TGGAAGTGGA TGGAATCAGC TTGGTGGGTG TGACACAGAA TTTTGCAGCA ACAGTTCTCA 
     2761 GAAACACCAA GGGCAACGTC AGATTTGTTA TTGGGCGGGA AAAACCAGGA CAAGTGAGCG 
     2821 AGGTTGCCCA GTTGATAAGC CAGACACTGG AACAGGAGAG GCGCCAGAGA GAGCTGCTGG 
     2881 AACAGCACTA TGCCCAGTAT GATGCCGACG ATGACGAGAA CACTGTGGCT GAATTGCAAG 
     2941 GAATGTCTGG CAACTGCAAT AACAATAACA ACTATTTTCT TAAGACAGGA GAATATGCCA 
     3001 CAGATGAAGA AGAAGATGAG GTAGGACCTG TCCTTCCTGG CAGCGACATG GCCATTGAAG 
     3061 TCTTTGAGCT GCCTGAGAAT GAGGACATGT TTTCCCCATC AGAACTGGAC ACAAGCAAGC 
     3121 TCAGTCACAA GTTCAAAGAG TTGCAAATCA AACATGCAGT TACAGAAGCA GAGATTCAAA 
     3181 AATTGAAGAC CAAGCTGCAG GCAGCAGAAA ATGAGAAAGT GAGGTGGGAA CTAGAAAAAA 
     3241 CCCAACTCCA ACAAAACATA GAAGAGAATA AGGAAAGAAT GTTGAAGTTG GAAAGCTACT 
     3301 GGATTGAGGC CCAAACATTA TGCCACACAG TGAATGAGCA TCTCAAAGAG ACTCAAAGCC 
     3361 AGTATCAGGC CTTGGAAAAG AAATACAACA AGGCAAAGAA GTTGATCAAG GATTTTCAAC 
     3421 AAAAAGAGCT TGATTTCATC AAAAGACAGG AAGCAGAAAG AAAGAAAATA GAAGATTTGG 
     3481 AAAAAGCTCA TCTTGTGGAA GTGCAAGGCC TCCAAGTGCG GATTAGAGAT TTGGAAGCTG 
     3541 AGGTATTCAG GCTACTGAAG CAAAATGGGA CTCAAGTTAA CAATAATAAC AACATCTTTG 
     3601 AGAGAAGAAC ATCTCTTGGT GAAGTCTCTA AAGGGGATAC CATGGAGAAC TTGGATGGCA 
     3661 AGCAGACATC TTGCCAAGAT GGCCTAAGTC AAGACTTGAA TGAAGCAGTC CCAGAGACAG 
     3721 AGCGCCTGGA TTCAAAAGCA CTGAAAACTC GAGCCCAGCT CTCTGTGAAG AACAGACGCC 
     3781 AGAGACCCTC TAGGACAAGA CTGTATGATA GTGTTAGTTC CACAGATGGG GAGGACAGTC 
     3841 TAGAGAGAAA GCCATCAAAC AGTTTCTATA ACCACATGCA TATTACCAAA TTACTTCCAC 
     3901 CTAAGGGTTT GAGAACGTCT TCTCCAGAAT CAGATTCTGG TGTTCCACCC CTCACCCCGG 
     3961 TGGATAGCAA TGTGCCCTTC TCGTCTGACC ACATAGCTGA ATTTCAAGAA GAACCACTGG 
     4021 ACCCAGAAAT GGGGCCTCTC TCCTCTATGT GGGGAGACAC TTCACTGTTT TCTACTTCAA 
     4081 AGTCTGATCA TGATGTGGAA GAATCTCCTT GCCATCACCA AACCACCAAC AAGAAAATAT 
     4141 TACAAGAAAA AGATGATGCC AAAGATCCCA AATCACTAAG GGCATCCAGT TCATTGGCGG 
     4201 TGCAAGGAGG AAAAATTAAG CGGAAGTTTG TGGATCTGGG GGCGCCTTTG CGAAGGAATT 
     4261 CCAGCAAGGG AAAGAAGTGG AAAGAAAAAG AAAAAGAAGC CAGTAGGTTT TCTGCAGGTA 
     4321 GCAGGATCTT CAGAGGCAGA CTGGAAAACT GGACACCCAA GCCATGTTCA ACAGCTCAGA 
     4381 CCTCCACTCG TTCCCCTTGC ATGCCTTTCT CATGGTTTAA TGACAGCCGG AAAGGATCCT 
     4441 ATTCCTTCAG GAACCTGCCT GCGCCTACAA GTTCCCTTCA GCCTTCTCCT GAGACTCTAA 
     4501 TTTCAGATAA AAAGGGGTCC AAGGTAGAAA ACACATGGAT TACAAAAGCA AACAAGAGAA 
     4561 ACCCAAATCC CTCCTCTTCT TCAATCTTTG GAAGGCATTC TCAACTTATG TCTGTAGTCT 
     4621 GGATCCAAGA AACCAATAAT TTTACCTTCA ATGATGACTT CAGTCCCAGC AGTACCAGTT 
     4681 CAGCAGACCT CAGCGGCTTA GGAGCAGAAC CTAAAACACC AGGGCTCTCT CAGTCCTTAG 
     4741 CACTGTCATC AGATGAGATC CTTGATGATG GACAGTCTCC CAAACACAGT CAGTGTCAGA 
     4801 ATCGGGCCGT TCAGGAATGG AGTGTGCAGC AGGTTTCTCA CTGGTTAATG AGCCTAAATC 
     4861 TGGAGCAGTA TGTATCTGAA TTCAGTGCCC AAAACATCAC TGGAGAACAG CTCCTGCAGT 
     4921 TGGATGGAAA TAAACTTAAG GCTCTTGGAA TGACAGCATC CCAGGACCGA GCAGTGGTCA 
     4981 AAAAGAAACT CAAGGAAATG AAGATGTCTC TAGAGAAGGC TCGGAAGGCC CAAGAGAAAA 
     5041 TGGAAAAACA AAGAGAAAAG CTAAGGAGAA AGGAGCAAGA GCAAATGCAG AGGAAGTCCA 
     5101 AAAAGACAGA AAAGATGACG TCAACTACAG CCGAGGGTGC TGGTGAGCAG ACGCGTACGC 
     5161 GGCCGCTCGA GCAGAAACTC ATCTCAGAAG AGGATCTGGC AGCAAATGAT ATCCTGGATT 
     5221 ACAAGGATGA CGACGATAAG GTTTAAACGG CCGGCCGCGG TCATAGCTGT TTCCTGAACA 
     5281 GATCCCGGGT GGCATCCCTG TGACCCCTCC CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC 
     5341 ACTCCAGTGC CCACCAGCCT TGTCCTAATA AAATTAAGTT GCATCATTTT GTCTGACTAG 
     5401 GTGTCCTTCT ATAATATTAT GGGGTGGAGG GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG 
     5461 AAGACAACCT GTAGGGCCTG CGGGGTCTAT TGGGAACCAA GCTGGAGTGC AGTGGCACAA 
     5521 TCTTGGCTCA CTGCAATCTC CGCCTCCTGG GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC 
     5581 GAGTTGTTGG GATTCCAGGC ATGCATGACC AGGCTCAGCT AATTTTTGTT TTTTTGGTAG 
     5641 AGACGGGGTT TCACCATATT GGCCAGGCTG GTCTCCAACT CCTAATCTCA GGTGATCTAC 
     5701 CCACCTTGGC CTCCCAAATT GCTGGGATTA CAGGCGTGAA CCACTGCTCC CTTCCCTGTC 
     5761 CTTCTGATTT TAAAATAACT ATACCAGCAG GAGGACGTCC AGACACAGCA TAGGCTACCT 
     5821 GGCCATGCCC AACCGGTGGG ACATTTGAGT TGCTTGCTTG GCACTGTCCT CTCATGCGTT 
     5881 GGGTCCACTC AGTAGATGCC TGTTGAATTG GGTACGCGGC CAGCGGCGAG CGGTATCAGC 
     5941 TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT 
     6001 GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT 
     6061 CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG 
     6121 AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC 
     6181 TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT 
     6241 GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA 
     6301 GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA 
     6361 TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA 
     6421 CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA 
     6481 CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT 
     6541 CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT 
     6601 TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT 
     6661 CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT 
     6721 GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC 
     6781 AATCTAAAGT ATATATGAGT AACCTGAGGC TATGGCAGGG CCTGCCGCCC CGACGTTGGC 
     6841 TGCGAGCCCT GGGCCTTCAC CCGAACTTGG GGGGTGGGGT GGGGAAAAGG AAGAAACGCG 
     6901 GGCGTATTGG CCCCAATGGG GTCTCGGTGG GGTATCGACA GAGTGCCAGC CCTGGGACCG 
     6961 AACCCCGCGT TTATGAACAA ACGACCCAAC ACCGTGCGTT TTATTCTGTC TTTTTATTGC 
     7021 CGTCATAGCG CGGGTTCCTT CCGGTATTGT CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT 
     7081 AGGGTGGGCG AAGAACTCCA GCATGAGATC CCCGCGCTGG AGGATCATCC AGCCGGCGTC 
     7141 CCGGAAAACG ATTCCGAAGC CCAACCTTTC ATAGAAGGCG GCGGTGGAAT CGAAATCTCG 
     7201 TGATGGCAGG TTGGGCGTCG CTTGGTCGGT CATTTCGAAC CCCAGAGTCC CGCTCAGAAG 
     7261 AACTCGTCAA GAAGGCGATA GAAGGCGATG CGCTGCGAAT CGGGAGCGGC GATACCGTAA 
     7321 AGCACGAGGA AGCGGTCAGC CCATTCGCCG CCAAGCTCTT CAGCAATATC ACGGGTAGCC 
     7381 AACGCTATGT CCTGATAGCG ATCCGCCACA CCCAGCCGGC CACAGTCGAT GAATCCAGAA 
     7441 AAGCGGCCAT TTTCCACCAT GATATTCGGC AAGCAGGCAT CGCCATGGGT CACGACGAGA 
     7501 TCCTCGCCGT CGGGCATGCT CGCCTTGAGC CTGGCGAACA GTTCGGCTGG CGCGAGCCCC 
     7561 TGATGCTCTT CGTCCAGATC ATCCTGATCG ACAAGACCGG CTTCCATCCG AGTACGTGCT 
     7621 CGCTCGATGC GATGTTTCGC TTGGTGGTCG AATGGGCAGG TAGCCGGATC AAGCGTATGC 
     7681 AGCCGCCGCA TTGCATCAGC CATGATGGAT ACTTTCTCGG CAGGAGCAAG GTGAGATGAC 
     7741 AGGAGATCCT GCCCCGGCAC TTCGCCCAAT AGCAGCCAGT CCCTTCCCGC TTCAGTGACA 
     7801 ACGTCGAGCA CAGCTGCGCA AGGAACGCCC GTCGTGGCCA GCCACGATAG CCGCGCTGCC 
     7861 TCGTCTTGCA GTTCATTCAG GGCACCGGAC AGGTCGGTCT TGACAAAAAG AACCGGGCGC 
     7921 CCCTGCGCTG ACAGCCGGAA CACGGCGGCA TCAGAGCAGC CGATTGTCTG TTGTGCCCAG 
     7981 TCATAGCCGA ATAGCCTCTC CACCCAAGCG GCCGGAGAAC CTGCGTGCAA TCCATCTTGT 
     8041 TCAATCATGC GAAACGATCC TCATCCTGTC TCTTGATCGA TCTTTGCAAA AGCCTAGGCC 
     8101 TCCAAAAAAG CCTCCTCACT ACTTCTGGAA TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC 
     8161 TGCATAAATA AAAAAAATTA GTCAGCCATG GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT 
     8221 TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG GACTATGGTT GCTGACTAAT TGAGATGCAT 
     8281 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCTGG TTGCTGACTA 
     8341 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCC 
     8401 TAACTGACAC ACATTCCACA GCTGGTTCTT TCCGCCTCAG GACTCTTCCT TTTTCAATAT 
     8461 TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG 
     8521 AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGCGCCC 
     8581 TGTAGCGGCG CATTAAGCGC GGCGGGTGTG GTGGTTACGC GCAGCGTGAC CGCTACACTT 
     8641 GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC 
     8701 GGCTTTCCCC GTCAAGCTCT AAATCGGGGG CTCCCTTTAG GGTTCCGATT TAGTGCTTTA 
     8761 CGGCACCTCG ACCCCAAAAA ACTTGATTAG GGTGATGGTT CACGTAGTGG GCCATCGCCC 
     8821 TGATAGACGG TTTTTCGCCC TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG 
     8881 TTCCAAACTG GAACAACACT CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT 
     8941 TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT 
     9001 TTT
//