LOCUS       RC229072                8844 bp    DNA     circular  SYN 
DEFINITION  KDM3A (Myc-DDK-tagged)-Human lysine (K)-specific demethylase 3A (KDM3A), transcript variant 2
ACCESSION   NM_001146688
VERSION     NM_001146688.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5001..5092 
                     /label="Myc-DDK"  
     misc_feature    5122..5707 
                     /label="PolyA signal"  
     misc_feature    5179..5198 
                     /label="Restriction Enzyme"  
     misc_feature    5983..6655 
                     /label="ColE1"  
     misc_feature    7085..7879 
                     /label="Kan/Neo"  
     misc_feature    7914..8216 
                     /label="SV40 ori"  
     misc_feature    8302..8779 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4983..4989 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4983 
                     /label="KDM3A"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGTGCTCACG CTCGGAGAAA GTTGGCCGGT ATTGGTGGGG AGGAGGTTTC 
     1081 TCAGTCTGTC CGCAGCCGAC GGCAGCGATG GCAGCCACGA CAGCTGGGAC GTGGAGCGCG 
     1141 TCGCCGAGTG GCCCTGGCTC TCCGGGACCA TTCGAGCTGT TTCCCACACC GACGTTACCA 
     1201 AGAAGGATCT GAAGGTGTGT GTGGAATTTG ATGGGGAATC TTGGAGGAAA AGAAGATGGA 
     1261 TAGAAGTCTA CAGCCTTCTA AGGAGAGCAT TTTTAGTAGA ACATAATTTG GTTTTAGCTG 
     1321 AACGAAAGTC ACCTGAAATT TCTGAACGAA TTGTACAGTG GCCTGCAATA ACGTACAAAC 
     1381 CTCTGTTGGA CAAAGCTGGT TTGGGATCCA TAACTTCTGT TCGCTTTCTG GGAGATCAAC 
     1441 AAAGAGTATT TCTTTCTAAA GACCTTTTGA AGCCTATACA GGATGTAAAC AGTCTTCGAC 
     1501 TTTCTCTTAC GGATAATCAG ATTGTCAGTA AAGAATTTCA AGCTTTGATT GTGAAGCATT 
     1561 TAGATGAAAG CCATCTTTTA AAAGGTGACA AAAACTTAGT TGGTTCAGAA GTAAAAATTT 
     1621 ATAGCTTGGA CCCATCTACT CAGTGGTTTT CAGCAACCGT TGTAAATGGA AACCCAGCAT 
     1681 CAAAAACTCT TCAAGTCAAC TGTGAGGAGA TTCCAGCACT GAAAATTGTT GATCCGTCAC 
     1741 TGATTCATGT TGAAGTTGTA CACGATAACC TTGTGACATG TGGTAATTCT GCAAGAATTG 
     1801 GAGCTGTAAA ACGCAAGTCT TCTGAGAATA ATGGAACCCT GGTTTCCAAA CAAGCAAAAT 
     1861 CTTGCTCTGA GGCCTCTCCC AGTATGTGTC CTGTGCAGTC TGTACCTACA ACAGTTTTTA 
     1921 AGGAGATACT GCTTGGCTGT ACTGCAGCAA CTCCACCTAG TAAGGACCCA AGACAGCAAA 
     1981 GTACTCCCCA GGCTGCCAAC TCTCCACCTA ACCTTGGAGC AAAAATTCCT CAAGGATGTC 
     2041 ATAAACAAAG TTTACCAGAG GAAATTTCTT CCTGTCTAAA TACAAAGTCT GAAGCTCTGA 
     2101 GAACAAAACC AGATGTCTGC AAAGCAGGGT TGCTCTCAAA GTCCTCTCAG ATTGGAACTG 
     2161 GAGACTTGAA AATTCTGACT GAGCCAAAAG GCAGCTGTAC TCAGCCTAAG ACAAACACTG 
     2221 ATCAGGAAAA CAGATTGGAG TCTGTTCCAC AAGCATTGAC TGGCCTTCCT AAGGAGTGCT 
     2281 TACCTACAAA GGCTTCTTCT AAGGCAGAAT TGGAAATTGC CAATCCTCCT GAACTGCAGA 
     2341 AGCACCTAGA ACATGCACCT TCCCCATCGG ATGTTTCAAA TGCACCAGAA GTGAAAGCAG 
     2401 GTGTCAATAG TGATAGCCCT AATAACTGTT CAGGAAAAAA GGTAGAACCT TCAGCTTTAG 
     2461 CTTGCCGATC ACAGAATTTA AAGGAATCTT CAGTAAAAGT AGATAATGAA AGCTGTTGTT 
     2521 CAAGAAGCAA CAATAAAATC CAGAATGCCC CATCCAGGAA GTCGGTTTTG ACAGACCCAG 
     2581 CTAAACTCAA AAAGCTGCAA CAGAGTGGCG AGGCCTTCGT ACAGGATGAT TCTTGTGTGA 
     2641 ACATCGTGGC ACAGTTGCCT AAATGCCGAG AGTGTCGCTT GGACAGTCTC CGCAAGGATA 
     2701 AGGAGCAACA GAAGGACTCA CCTGTGTTTT GCCGCTTCTT TCACTTCAGG AGGTTACAAT 
     2761 TCAACAAACA TGGTGTGTTG CGGGTAGAAG GCTTCTTAAC ACCAAACAAG TATGACAATG 
     2821 AAGCAATTGG CTTGTGGTTA CCTTTAACCA AAAACGTTGT GGGGATTGAT TTGGACACAG 
     2881 CAAAGTACAT CTTGGCCAAC ATTGGAGACC ACTTCTGTCA AATGGTGATT TCTGAAAAGG 
     2941 AAGCTATGTC AACTATTGAG CCACACAGAC AGGTTGCTTG GAAGCGAGCT GTCAAAGGTG 
     3001 TTCGAGAAAT GTGTGATGTG TGCGACACCA CCATCTTCAA CCTGCACTGG GTGTGTCCTC 
     3061 GGTGTGGGTT TGGAGTATGT GTGGACTGCT ACCGGATGAA GAGAAAGAAT TGCCAACAGG 
     3121 GTGCTGCTTA CAAGACTTTC TCTTGGCTAA AATGTGTGAA GAGTCAGATA CATGAACCAG 
     3181 AGAACTTAAT GCCCACACAG ATCATTCCTG GAAAAGCACT CTATGATGTT GGAGACATTG 
     3241 TTCATTCTGT AAGAGCGAAA TGGGGAATAA AGGCAAACTG CCCTTGTTCA AACAGGCAAT 
     3301 TCAAACTCTT TTCAAAGCCA GCCTCAAAGG AAGACCTAAA ACAGACTTCT TTAGCTGGAG 
     3361 AAAAACCGAC TCTTGGTGCA GTGCTCCAGC AGAATCCCTC AGTGTTGGAG CCAGCAGCTG 
     3421 TGGGTGGGGA AGCAGCCTCC AAGCCAGCCG GCAGCATGAA GCCTGCCTGT CCAGCCAGCA 
     3481 CATCTCCTCT AAACTGGCTG GCCGACCTAA CCAGCGGGAA TGTCAACAAG GAAAACAAGG 
     3541 AAAAACAACC AACAATGCCA ATTTTAAAGA ATGAAATCAA ATGCCTTCCA CCCCTCCCAC 
     3601 CTTTAAGCAA ATCCAGCACA GTCCTCCATA CGTTTAACAG CACAATTTTG ACACCCGTAA 
     3661 GCAACAACAA TTCTGGTTTC CTCCGGAATC TCTTGAATTC TTCTACAGGA AAGACAGAAA 
     3721 ATGGACTCAA GAATACACCA AAAATCCTTG ATGACATCTT TGCCTCTTTG GTGCAAAATA 
     3781 AGACGACTTC TGATTTATCT AAGAGGCCTC AAGGACTAAC CATCAAGCCC AGCATTCTGG 
     3841 GCTTTGACAC TCCTCACTAT TGGCTTTGTG ATAATCGCTT GCTGTGCTTG CAAGACCCCA 
     3901 ACAATAAGAG CAACTGGAAT GTGTTTAGGG AGTGCTGGAA ACAAGGGCAG CCAGTGATGG 
     3961 TGTCTGGAGT GCATCATAAA TTGAACTCTG AACTTTGGAA ACCTGAATCC TTCAGGAAAG 
     4021 AGTTTGGTGA GCAGGAAGTA GACCTAGTTA ATTGTAGGAC CAATGAAATC ATCACAGGAG 
     4081 CCACAGTAGG AGACTTCTGG GATGGATTTG AAGATGTTCC AAATCGTTTG AAAAATGAAA 
     4141 AAGAACCAAT GGTGTTGAAA CTTAAGGACT GGCCACCAGG AGAAGATTTT AGAGATATGA 
     4201 TGCCTTCCAG GTTTGATGAT CTGATGGCCA ACATTCCACT GCCCGAGTAC ACAAGGCGAG 
     4261 ATGGCAAACT GAATTTGGCC TCTAGGCTGC CAAACTACTT TGTTCGGCCA GATCTGGGCC 
     4321 CCAAGATGTA TAATGCTTAT GGATTAATCA CTCCTGAAGA TCGGAAATAT GGAACAACAA 
     4381 ATCTTCACTT AGATGTATCT GATGCAGCTA ATGTCATGGT CTATGTGGGA ATTCCCAAAG 
     4441 GACAGTGTGA GCAAGAAGAA GAAGTCCTTA AGACCATCCA AGATGGAGAT TCTGACGAAC 
     4501 TCACAATAAA GCGATTTATT GAAGGAAAAG AGAAGCCAGG AGCACTGTGG CACATATATG 
     4561 CTGCAAAGGA CACGGAGAAG ATAAGGGAAT TTCTTAAAAA GGTATCAGAA GAGCAAGGTC 
     4621 AAGAAAACCC AGCAGACCAC GATCCTATTC ATGATCAAAG CTGGTATTTA GACCGATCAT 
     4681 TAAGAAAACG TCTTCATCAA GAGTATGGAG TTCAAGGCTG GGCTATTGTA CAGTTTCTTG 
     4741 GGGATGTGGT GTTTATCCCG GCAGGAGCTC CACATCAGGT TCATAACTTA TATAGCTGCA 
     4801 TCAAAGTGGC TGAAGATTTT GTTTCTCCAG AGCATGTTAA ACACTGCTTC TGGCTTACTC 
     4861 AGGAATTCCG ATATCTGTCA CAGACTCATA CCAATCACGA AGATAAATTA CAGGTGAAGA 
     4921 ATGTTATCTA CCATGCAGTG AAAGATGCAG TTGCTATGCT GAAAGCCAGT GAATCCAGTT 
     4981 TTGGCAAACC TACGCGTACG CGGCCGCTCG AGCAGAAACT CATCTCAGAA GAGGATCTGG 
     5041 CAGCAAATGA TATCCTGGAT TACAAGGATG ACGACGATAA GGTTTAAACG GCCGGCCGCG 
     5101 GTCATAGCTG TTTCCTGAAC AGATCCCGGG TGGCATCCCT GTGACCCCTC CCCAGTGCCT 
     5161 CTCCTGGCCC TGGAAGTTGC CACTCCAGTG CCCACCAGCC TTGTCCTAAT AAAATTAAGT 
     5221 TGCATCATTT TGTCTGACTA GGTGTCCTTC TATAATATTA TGGGGTGGAG GGGGGTGGTA 
     5281 TGGAGCAAGG GGCAAGTTGG GAAGACAACC TGTAGGGCCT GCGGGGTCTA TTGGGAACCA 
     5341 AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT CCGCCTCCTG GGTTCAAGCG 
     5401 ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG CATGCATGAC CAGGCTCAGC 
     5461 TAATTTTTGT TTTTTTGGTA GAGACGGGGT TTCACCATAT TGGCCAGGCT GGTCTCCAAC 
     5521 TCCTAATCTC AGGTGATCTA CCCACCTTGG CCTCCCAAAT TGCTGGGATT ACAGGCGTGA 
     5581 ACCACTGCTC CCTTCCCTGT CCTTCTGATT TTAAAATAAC TATACCAGCA GGAGGACGTC 
     5641 CAGACACAGC ATAGGCTACC TGGCCATGCC CAACCGGTGG GACATTTGAG TTGCTTGCTT 
     5701 GGCACTGTCC TCTCATGCGT TGGGTCCACT CAGTAGATGC CTGTTGAATT GGGTACGCGG 
     5761 CCAGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG 
     5821 GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA 
     5881 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG 
     5941 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC 
     6001 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC 
     6061 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC 
     6121 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG 
     6181 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC 
     6241 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA 
     6301 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC 
     6361 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC 
     6421 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG 
     6481 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC 
     6541 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA 
     6601 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAACCTGAGG CTATGGCAGG 
     6661 GCCTGCCGCC CCGACGTTGG CTGCGAGCCC TGGGCCTTCA CCCGAACTTG GGGGGTGGGG 
     6721 TGGGGAAAAG GAAGAAACGC GGGCGTATTG GCCCCAATGG GGTCTCGGTG GGGTATCGAC 
     6781 AGAGTGCCAG CCCTGGGACC GAACCCCGCG TTTATGAACA AACGACCCAA CACCGTGCGT 
     6841 TTTATTCTGT CTTTTTATTG CCGTCATAGC GCGGGTTCCT TCCGGTATTG TCTCCTTCCG 
     6901 TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC GAAGAACTCC AGCATGAGAT CCCCGCGCTG 
     6961 GAGGATCATC CAGCCGGCGT CCCGGAAAAC GATTCCGAAG CCCAACCTTT CATAGAAGGC 
     7021 GGCGGTGGAA TCGAAATCTC GTGATGGCAG GTTGGGCGTC GCTTGGTCGG TCATTTCGAA 
     7081 CCCCAGAGTC CCGCTCAGAA GAACTCGTCA AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA 
     7141 TCGGGAGCGG CGATACCGTA AAGCACGAGG AAGCGGTCAG CCCATTCGCC GCCAAGCTCT 
     7201 TCAGCAATAT CACGGGTAGC CAACGCTATG TCCTGATAGC GATCCGCCAC ACCCAGCCGG 
     7261 CCACAGTCGA TGAATCCAGA AAAGCGGCCA TTTTCCACCA TGATATTCGG CAAGCAGGCA 
     7321 TCGCCATGGG TCACGACGAG ATCCTCGCCG TCGGGCATGC TCGCCTTGAG CCTGGCGAAC 
     7381 AGTTCGGCTG GCGCGAGCCC CTGATGCTCT TCGTCCAGAT CATCCTGATC GACAAGACCG 
     7441 GCTTCCATCC GAGTACGTGC TCGCTCGATG CGATGTTTCG CTTGGTGGTC GAATGGGCAG 
     7501 GTAGCCGGAT CAAGCGTATG CAGCCGCCGC ATTGCATCAG CCATGATGGA TACTTTCTCG 
     7561 GCAGGAGCAA GGTGAGATGA CAGGAGATCC TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG 
     7621 TCCCTTCCCG CTTCAGTGAC AACGTCGAGC ACAGCTGCGC AAGGAACGCC CGTCGTGGCC 
     7681 AGCCACGATA GCCGCGCTGC CTCGTCTTGC AGTTCATTCA GGGCACCGGA CAGGTCGGTC 
     7741 TTGACAAAAA GAACCGGGCG CCCCTGCGCT GACAGCCGGA ACACGGCGGC ATCAGAGCAG 
     7801 CCGATTGTCT GTTGTGCCCA GTCATAGCCG AATAGCCTCT CCACCCAAGC GGCCGGAGAA 
     7861 CCTGCGTGCA ATCCATCTTG TTCAATCATG CGAAACGATC CTCATCCTGT CTCTTGATCG 
     7921 ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA GCCTCCTCAC TACTTCTGGA ATAGCTCAGA 
     7981 GGCCGAGGCG GCCTCGGCCT CTGCATAAAT AAAAAAAATT AGTCAGCCAT GGGGCGGAGA 
     8041 ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG GATGGGCGGA GTTAGGGGCG GGACTATGGT 
     8101 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     8161 TCCACACCTG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     8221 GCCTGGGGAC TTTCCACACC CTAACTGACA CACATTCCAC AGCTGGTTCT TTCCGCCTCA 
     8281 GGACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA 
     8341 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA 
     8401 AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG 
     8461 CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT 
     8521 TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA 
     8581 GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT 
     8641 TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG 
     8701 TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT 
     8761 TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT 
     8821 TAACAAAAAT TTAACGCGAA TTTT
//