LOCUS       RC229071                8640 bp    DNA     circular  SYN 
DEFINITION  PPP1R9A (Myc-DDK-tagged)-Human protein phosphatase 1, regulatory (inhibitor) subunit 9A (PPP1R9A), transcript variant 3
ACCESSION   NM_001166162
VERSION     NM_001166162.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4797..4888 
                     /label="Myc-DDK"  
     misc_feature    4918..5503 
                     /label="PolyA signal"  
     misc_feature    4975..4994 
                     /label="Restriction Enzyme"  
     misc_feature    5779..6451 
                     /label="ColE1"  
     misc_feature    6881..7675 
                     /label="Kan/Neo"  
     misc_feature    7710..8012 
                     /label="SV40 ori"  
     misc_feature    8098..8575 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4779..4785 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4779 
                     /label="PPP1R9A"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTTGAAAACT GAGTCTTCAG GTGAACGAAC CACTCTCAGA AGTGCCTCTC 
     1081 CTCACAGGAA TGCATATCGA ACTGAGTTTC AGGCACTGAA AAGTACCTTT GACAAACCCA 
     1141 AGTCAGATGG GGAACAAAAA ACAAAAGAAG GTGAGGGCTC CCAGCAGAGC AGGGGGAGGA 
     1201 AATATGGCTC CAATGTCAAC AGAATTAAAA ACCTATTTAT GCAGATGGGT ATGGAACCCA 
     1261 ACGAGAATGC TGCAGTCATT GCCAAAACAA GGGGGAAAGG TGGACATTCA TCTCCTCAGA 
     1321 GAAGAATGAA GCCCAAAGAA TTTCTGGAAA AAACAGATGG CTCAGTTGTT AAGTTGGAGT 
     1381 CTTCTGTTTC TGAACGAATT AGTAGATTTG ACACTATGTA CGATGGCCCT TCATATTCCA 
     1441 AGTTCACTGA GACTCGAAAG ATGTTTGAGA GAAGTGTGCA TGAATCAGGA CAGAACAACC 
     1501 GCTATTCCCC AAAGAAAGAG AAAGCTGGAG GGAGTGAACC TCAGGATGAA TGGGGAGGTT 
     1561 CCAAGTCCAA CAGAGGCAGT ACTGATTCCT TGGACAGCCT TAGCTCCCGA ACTGAGGCTG 
     1621 TCTCCCCAAC TGTGAGTCAA CTGAGTGCAG TATTTGAGAA CACTGATTCT CCCAGTGCCA 
     1681 TCATTTCTGA GAAGGCTGAA AACAATGAAT ACTCAGTGAC TGGGCATTAT CCCTTGAATT 
     1741 TACCATCTGT TACTGTTACA AATCTTGACA CATTTGGTCA CCTGAAGGAT TCTAATTCCT 
     1801 GGCCTCCTTC AAACAAGCGA GGTGTTGATA CAGAGGATGC TCACAAGAGT AATGCAACTC 
     1861 CAGTACCAGA AGTGGCTTCT AAAAGTACCT CTCTAGCTTC GATACCTGGT GAAGAGATCC 
     1921 AGCAGAGCAA GGAACCCGAG GACTCCACAT CTAATCAACA GACTCCCGAC AGCATTGACA 
     1981 AAGATGGTCC TGAAGAACCT TGTGCTGAAA GTAAGGCAGT GCCAAAGTCC GAAATCCCTT 
     2041 CACCACAAAG CCAACTGTTA GAAGATGCTG AAGCTAATTT GGTTGGAAGG GAGGCAGCAA 
     2101 AGCAACAGAG GAAAGAACTT GCAGGTGGTG ATTTCACCTC TCCTGATGCT TCTGCATCCA 
     2161 GTTGTGGAAA AGAAGTACCT GAAGATTCAA ATAATTTTGA TGGTTCCCAT GTGTACATGC 
     2221 ACAGTGACTA TAATGTGTAT AGGGTGAGAT CCAGGTATAA TTCAGACTGG GGAGAGACAG 
     2281 GCACTGAGCA GGATGAGGAG GAAGATAGTG ATGAGAACAG TTACTATCAG CCTGATATGG 
     2341 AGTACTCGGA AATTGTTGGA TTGCCAGAAG AAGAAGAAAT CCCAGCAAAT AGGAAAATTA 
     2401 AGTTTAGTAG TGCTCCTATT AAGGTTTTCA ACACATACTC CAATGAAGAC TATGACAGGA 
     2461 GAAATGACGA AGTTGACCCT GTGGCTGCTT CAGCTGAGTA TGAACTTGAA AAACGTGTAG 
     2521 AAAAGCTGGA ACTTTTCCCA GTGGAGCTAG AGAAAGATGA GGATGGTCTT GGTATAAGTA 
     2581 TTATTGGAAT GGGTGTTGGA GCAGATGCTG GACTTGAAAA GCTGGGAATA TTCGTCAAGA 
     2641 CAGTAACAGA AGGTGGTGCT GCTCAACGGG ATGGCAGAAT ACAAGTCAAT GACCAGATTG 
     2701 TGGAAGTGGA TGGAATCAGC TTGGTGGGTG TGACACAGAA TTTTGCAGCA ACAGTTCTCA 
     2761 GAAACACCAA GGGCAACGTC AGATTTGTTA TTGGGCGGGA AAAACCAGGA CAAGTGAGCG 
     2821 AGGTTGCCCA GTTGATAAGC CAGACACTGG AACAGGAGAG GCGCCAGAGA GAGCTGCTGG 
     2881 AACAGCACTA TGCCCAGTAT GATGCCGACG ATGACGAGAC AGGAGAATAT GCCACAGATG 
     2941 AAGAAGAAGA TGAGGTAGGA CCTGTCCTTC CTGGCAGCGA CATGGCCATT GAAGTCTTTG 
     3001 AGCTGCCTGA GAATGAGGAC ATGTTTTCCC CATCAGAACT GGACACAAGC AAGCTCAGTC 
     3061 ACAAGTTCAA AGAGTTGCAA ATCAAACATG CAGTTACAGA AGCAGAGATT CAAAAATTGA 
     3121 AGACCAAGCT GCAGGCAGCA GAAAATGAGA AAGTGAGGTG GGAACTAGAA AAAACCCAAC 
     3181 TCCAACAAAA CATAGAAGAG AATAAGGAAA GAATGTTGAA GTTGGAAAGC TACTGGATTG 
     3241 AGGCCCAAAC ATTATGCCAC ACAGTGAATG AGCATCTCAA AGAGACTCAA AGCCAGTATC 
     3301 AGGCCTTGGA AAAGAAATAC AACAAGGCAA AGAAGTTGAT CAAGGATTTT CAACAAAAAG 
     3361 AGCTTGATTT CATCAAAAGA CAGGAAGCAG AAAGAAAGAA AATAGAAGAT TTGGAAAAAG 
     3421 CTCATCTTGT GGAAGTGCAA GGCCTCCAAG TGCGGATTAG AGATTTGGAA GCTGAGGTAT 
     3481 TCAGGCTACT GAAGCAAAAT GGGACTCAAG TTAACAATAA TAACAACATC TTTGAGAGAA 
     3541 GAACATCTCT TGGTGAAGTC TCTAAAGGGG ATACCATGGA GAACTTGGAT GGCAAGCAGA 
     3601 CATCTTGCCA AGATGGCCTA AGTCAAGACT TGAATGAAGC AGTCCCAGAG ACAGAGCGCC 
     3661 TGGATTCAAA AGCACTGAAA ACTCGAGCCC AGCTCTCTGT GAAGAACAGA CGCCAGAGAC 
     3721 CCTCTAGGAC AAGACTGTAT GATAGTGTTA GTTCCACAGA TGGGGAGGAC AGTCTAGAGA 
     3781 GAAAGCCATC AAACAGTTTC TATAACCACA TGCATATTAC CAAATTACTT CCACCTAAGG 
     3841 GTTTGAGAAC GTCTTCTCCA GAATCAGATT CTGGTGTTCC ACCCCTCACC CCGGTGGATA 
     3901 GCAATGTGCC CTTCTCGTCT GACCACATAG CTGAATTTCA AGAAGAACCA CTGGACCCAG 
     3961 AAATGGGGCC TCTCTCCTCT ATGTGGGGAG ACACTTCACT GTTTTCTACT TCAAAGTCTG 
     4021 ATCATGATGT GGAAGAATCT CCTTGCCATC ACCAAACCAC CAACAAGAAA ATATTACAAG 
     4081 AAAAAGATGA TGCCAAAGAT CCCAAATCAC TAAGGGCATC CAGTTCATTG GCGGTGCAAG 
     4141 GAGGAAAAAT TAAGCGGAAG TTTGTGGATC TGGGGGCGCC TTTGCGAAGG AATTCCAACA 
     4201 AGGGAAAGAA GTGGAAAGAA AAAGAAAAAG AAGCCAGTAG GTTTTCTGCA GGTAGCAGGA 
     4261 TCTTCAGAGG CAGACTGGAA AACTGGACAC CCAAGCCATG TTCAACAGCT CAGACCTCCA 
     4321 CTCGTTCCCC TTGCATGCCT TTCTCATGGT TTAATGACAG CCGGAAAGGA TCCTATTCCT 
     4381 TCAGGAACCT GCCTGCGCCT ACAAGTTCCC TTCAGCCTTC TCCTGAGACT CTAATTTCAG 
     4441 ATAAAAAGGG GTCCAAGAAT TTTACCTTCA ATGATGACTT CAGTCCCAGC AGTACCAGTT 
     4501 CAGCAGACCT CAGCGGCTTA GGAGCAGAAC CTAAAACACC AGGGCTCTCT CAGTCCTTAG 
     4561 CACTGTCATC AGATGAGGCT CTTGGAATGA CAGCATCCCA GGACCGAGCA GTGGTCAAAA 
     4621 AGAAACTCAA GGAAATGAAG ATGTCTCTAG AGAAGGCTCG GAAGGCCCAA GAGAAAATGG 
     4681 AAAAACAAAG AGAAAAGCTA AGGAGAAAGG AGCAAGAGCA AATGCAGAGG AAGTCCAAAA 
     4741 AGACAGAAAA GATGACGTCA ACTACAGCCG AGGGTGCTGG TGAGCAGACG CGTACGCGGC 
     4801 CGCTCGAGCA GAAACTCATC TCAGAAGAGG ATCTGGCAGC AAATGATATC CTGGATTACA 
     4861 AGGATGACGA CGATAAGGTT TAAACGGCCG GCCGCGGTCA TAGCTGTTTC CTGAACAGAT 
     4921 CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA GTGCCTCTCC TGGCCCTGGA AGTTGCCACT 
     4981 CCAGTGCCCA CCAGCCTTGT CCTAATAAAA TTAAGTTGCA TCATTTTGTC TGACTAGGTG 
     5041 TCCTTCTATA ATATTATGGG GTGGAGGGGG GTGGTATGGA GCAAGGGGCA AGTTGGGAAG 
     5101 ACAACCTGTA GGGCCTGCGG GGTCTATTGG GAACCAAGCT GGAGTGCAGT GGCACAATCT 
     5161 TGGCTCACTG CAATCTCCGC CTCCTGGGTT CAAGCGATTC TCCTGCCTCA GCCTCCCGAG 
     5221 TTGTTGGGAT TCCAGGCATG CATGACCAGG CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA 
     5281 CGGGGTTTCA CCATATTGGC CAGGCTGGTC TCCAACTCCT AATCTCAGGT GATCTACCCA 
     5341 CCTTGGCCTC CCAAATTGCT GGGATTACAG GCGTGAACCA CTGCTCCCTT CCCTGTCCTT 
     5401 CTGATTTTAA AATAACTATA CCAGCAGGAG GACGTCCAGA CACAGCATAG GCTACCTGGC 
     5461 CATGCCCAAC CGGTGGGACA TTTGAGTTGC TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG 
     5521 TCCACTCAGT AGATGCCTGT TGAATTGGGT ACGCGGCCAG CGGCGAGCGG TATCAGCTCA 
     5581 CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG 
     5641 AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA 
     5701 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA 
     5761 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC 
     5821 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC 
     5881 GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT 
     5941 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG 
     6001 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG 
     6061 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA 
     6121 CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG 
     6181 AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT 
     6241 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT 
     6301 TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG 
     6361 ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT 
     6421 CTAAAGTATA TATGAGTAAC CTGAGGCTAT GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC 
     6481 GAGCCCTGGG CCTTCACCCG AACTTGGGGG GTGGGGTGGG GAAAAGGAAG AAACGCGGGC 
     6541 GTATTGGCCC CAATGGGGTC TCGGTGGGGT ATCGACAGAG TGCCAGCCCT GGGACCGAAC 
     6601 CCCGCGTTTA TGAACAAACG ACCCAACACC GTGCGTTTTA TTCTGTCTTT TTATTGCCGT 
     6661 CATAGCGCGG GTTCCTTCCG GTATTGTCTC CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG 
     6721 GTGGGCGAAG AACTCCAGCA TGAGATCCCC GCGCTGGAGG ATCATCCAGC CGGCGTCCCG 
     6781 GAAAACGATT CCGAAGCCCA ACCTTTCATA GAAGGCGGCG GTGGAATCGA AATCTCGTGA 
     6841 TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT TTCGAACCCC AGAGTCCCGC TCAGAAGAAC 
     6901 TCGTCAAGAA GGCGATAGAA GGCGATGCGC TGCGAATCGG GAGCGGCGAT ACCGTAAAGC 
     6961 ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA AGCTCTTCAG CAATATCACG GGTAGCCAAC 
     7021 GCTATGTCCT GATAGCGATC CGCCACACCC AGCCGGCCAC AGTCGATGAA TCCAGAAAAG 
     7081 CGGCCATTTT CCACCATGAT ATTCGGCAAG CAGGCATCGC CATGGGTCAC GACGAGATCC 
     7141 TCGCCGTCGG GCATGCTCGC CTTGAGCCTG GCGAACAGTT CGGCTGGCGC GAGCCCCTGA 
     7201 TGCTCTTCGT CCAGATCATC CTGATCGACA AGACCGGCTT CCATCCGAGT ACGTGCTCGC 
     7261 TCGATGCGAT GTTTCGCTTG GTGGTCGAAT GGGCAGGTAG CCGGATCAAG CGTATGCAGC 
     7321 CGCCGCATTG CATCAGCCAT GATGGATACT TTCTCGGCAG GAGCAAGGTG AGATGACAGG 
     7381 AGATCCTGCC CCGGCACTTC GCCCAATAGC AGCCAGTCCC TTCCCGCTTC AGTGACAACG 
     7441 TCGAGCACAG CTGCGCAAGG AACGCCCGTC GTGGCCAGCC ACGATAGCCG CGCTGCCTCG 
     7501 TCTTGCAGTT CATTCAGGGC ACCGGACAGG TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC 
     7561 TGCGCTGACA GCCGGAACAC GGCGGCATCA GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA 
     7621 TAGCCGAATA GCCTCTCCAC CCAAGCGGCC GGAGAACCTG CGTGCAATCC ATCTTGTTCA 
     7681 ATCATGCGAA ACGATCCTCA TCCTGTCTCT TGATCGATCT TTGCAAAAGC CTAGGCCTCC 
     7741 AAAAAAGCCT CCTCACTACT TCTGGAATAG CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC 
     7801 ATAAATAAAA AAAATTAGTC AGCCATGGGG CGGAGAATGG GCGGAACTGG GCGGAGTTAG 
     7861 GGGCGGGATG GGCGGAGTTA GGGGCGGGAC TATGGTTGCT GACTAATTGA GATGCATGCT 
     7921 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCTGGTTG CTGACTAATT 
     7981 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCCTAA 
     8041 CTGACACACA TTCCACAGCT GGTTCTTTCC GCCTCAGGAC TCTTCCTTTT TCAATATTAT 
     8101 TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA 
     8161 AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA CGCGCCCTGT 
     8221 AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC 
     8281 AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC 
     8341 TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG 
     8401 CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA 
     8461 TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC 
     8521 CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA AGGGATTTTG 
     8581 CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//