LOCUS       RC229061                8130 bp    DNA     circular  SYN 
DEFINITION  SAP130 (Myc-DDK-tagged)-Human Sin3A-associated protein, 130kDa (SAP130), transcript variant 1
ACCESSION   NM_001145928
VERSION     NM_001145928.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4287..4378 
                     /label="Myc-DDK"  
     misc_feature    4408..4993 
                     /label="PolyA signal"  
     misc_feature    4465..4484 
                     /label="Restriction Enzyme"  
     misc_feature    5269..5941 
                     /label="ColE1"  
     misc_feature    6371..7165 
                     /label="Kan/Neo"  
     misc_feature    7200..7502 
                     /label="SV40 ori"  
     misc_feature    7588..8065 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4269..4275 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4269 
                     /label="SAP130"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGCCCTCCG CGGCACCCCC AGGCCGGCGA GATAGAAGCG GGCGGTGCGG 
     1081 GCGGCGGGCG GCGGCTACAG GTGGAAATGA GTTCTCAACA GTTTCCTCGG TTAGGAGCCC 
     1141 CTTCTACCGG GCTGAGCCAG GCCCCTTCTC AGATTGCAAA CAGTGGTTCT GCTGGATTGA 
     1201 TAAACCCAGC TGCTACAGTC AATGATGAAT CTGGTCGAGA TTCTGAAGTC AGTGCCAGGG 
     1261 AGCACATGAG TTCCAGCAGC TCCCTCCAGT CCCGGGAGGA GAAGCAAGAG CCTGTTGTGG 
     1321 TAAGGCCCTA TCCACAGGTG CAGATGTTGT CGACACACCA TGCTGTCGCA TCAGCCACAC 
     1381 CTGTTGCAGT GACAGCCCCG CCAGCACACC TGACGCCAGC AGTGCCACTT TCATTTTCGG 
     1441 AGGGACTTAT GAAGCCGCCC CCGAAGCCCA CCATGCCTAG CCGTCCCATT GCTCCTGCTC 
     1501 CACCTTCTAC CCTGTCACTT CCCCCCAAGG TTCCAGGGCA GGTTACCGTT ACCATGGAGA 
     1561 GTAGCATCCC TCAAGCTTCA GCCATTCCTG TGGCAACAAT CAGTGGACAA CAGGGCCATC 
     1621 CCAGTAACCT GCATCACATC ATGACTACAA ATGTGCAAAT GTCTATCATC CGCAGCAATG 
     1681 CTCCTGGGCC CCCTCTTCAC ATTGGAGCTT CTCATTTACC TCGAGGTGCA GCTGCTGCTG 
     1741 CTGTGATGTC CAGTTCTAAA GTAACCACAG TCCTGAGGCC GACCTCACAG CTGCCAAATG 
     1801 CTGCTACTGC TCAGCCAGCA GTACAGCACA TCATTCACCA ACCAATCCAG TCTCGGCCAC 
     1861 CTGTGACCAC CTCCAATGCC ATCCCTCCTG CTGTGGTAGC AACTGTCTCA GCCACCAGAG 
     1921 CTCAGTCTCC AGTCATCACT ACGACAGCGG CGCATGCTAC TGATTCAGCA CTTAGTAGGC 
     1981 CAACCTTGTC TATCCAGCAT CCTCCATCTG CAGCAATCAG TATTCAGCGT CCTGCCCAGT 
     2041 CACGAGATGT CACAACAAGA ATCACACTAC CATCTCACCC TGCATTAGGG ACGCCAAAAC 
     2101 AGCAGCTTCA TACAATGGCT CAGAAAACAA TCTTCAGTAC TGGCACGCCA GTGGCTGCAG 
     2161 CCACAGTAGC ACCTATTTTG GCAACCAACA CCATTCCTTC AGCGACCACA GCTGGATCTG 
     2221 TGTCACACAC GCAAGCTCCC ACAAGTACCA TTGTTACCAT GACAGTACCC TCCCATTCCT 
     2281 CCCATGCTAC TGCTGTGACC ACCTCAAACA TCCCAGTCGC CAAGGTGGTG CCCCAGCAGA 
     2341 TCACGCACAC TTCTCCTCGG ATCCAGCCAG ACTACCCTGC CGAGAGGAGT AGCCTGATTC 
     2401 CCATCTCCGG ACATCGGGCC TCTCCCAATC CTGTGGCCAT GGAAACCCGA AGTGACAACA 
     2461 GACCGTCTGT TCCCGTTCAG TTCCAATATT TTTTGCCAAC TTACCCCCCT TCTGCATACC 
     2521 CACTGGCGGC ACATACCTAC ACCCCAATCA CCAGTTCCGT GTCCACTATC CGACAGTATC 
     2581 CAGTTTCAGC TCAGGCTCCA AACTCTGCCA TCACAGCTCA GACTGGTGTT GGGGTAGCGT 
     2641 CTACCGTCCA CCTAAACCCC ATGCAGTTGA TGACAGTGGA TGCATCGCAT GCTCGACATA 
     2701 TTCAAGGGAT CCAGCCAGCA CCCATCAGTA CCCAGGGTAT CCAGCCGGCC CCCATTGGGA 
     2761 CCCCAGGGAT ACAGCCTGCA CCACTTGGCA CACAGGGAAT TCACTCAGCA ACCCCAATCA 
     2821 ACACACAAGG GCTTCAGCCT GCACCTATGG GTACTCAGCA GCCTCAGCCT GAAGGAAAGA 
     2881 CTTCAGCAGT GGTGTTGGCA GATGGAGCCA CAATTGTGGC CAACCCTATT AGCAATCCAT 
     2941 TCAGTGCTGC TCCAGCAGCA ACAACCGTGG TGCAGACCCA CAGCCAGAGT GCTAGCACCA 
     3001 ACGCTCCCGC CCAGGGCTCA TCGCCACGGC CAAGCATACT CCGGAAGAAA CCTGCCACAG 
     3061 ATGGAATGGC AGTTCGGAAA ACCCTCATTC CTCCTCAGCC TCCTGATGTT GCTAGTCCTC 
     3121 GAGTGGAAAG CTCTATGCGG AGTACGTCTG GGTCACCTAG GCCTGCAGGT GCCAAACCCA 
     3181 AGTCTGAAAT CCACGTGTCT ATGGCCACTC CGGTCACTGT GTCCATGGAG ACTGTATCCA 
     3241 ATCAAAATAA TGATCAGCCT ACCATTGCCG TCCCTCCAAC TGCCCAGCAG CCCCCACCGA 
     3301 CCATTCCAAC TATGATTGCA GCAGCCAGTC CCCCGTCACA ACCAGCCGTT GCCCTTTCAA 
     3361 CCATTCCTGG AGCGGTCCCC ATCACTCCAC CCATCACCAC CATTGCAGCT GCACCACCTC 
     3421 CATCAGTCAC TGTGGGTGGC AGTCTTTCCT CCGTCTTGGG CCCTCCCGTT CCTGAAATTA 
     3481 AAGTGAAAGA AGAAGTAGAA CCAATGGATA TCATGAGGCC AGTTTCTGCA GTTCCTCCAC 
     3541 TGGCTACCAA CACTGTGTCT CCATCTCTTG CATTGCTGGC AAACAACTTG TCCATGCCTA 
     3601 CAAGTGACCT ACCACCTGGT GCCTCCCCAA GGAAAAAGCC TCGAAAGCAA CAGCATGTGA 
     3661 TCTCAACAGA AGAAGGTGAC ATGATGGAGA CAAACAGCAC TGATGATGAG AAGTCCACTG 
     3721 CCAAGAGTCT TCTGGTGAAG GCTGAGAAGC GCAAGTCTCC TCCCAAGGAG TATATTGATG 
     3781 AGGAAGGTGT GAGATATGTC CCAGTGCGTC CAAGACCCCC CATTACTTTG CTTCGTCACT 
     3841 ATCGGAACCC CTGGAAAGCT GCTTACCACC ACTTTCAGAG GTACAGTGAC GTCCGGGTCA 
     3901 AAGAGGAGAA GAAAGCTATG CTGCAGGAAA TAGCTAATCA GAAAGGAGTA TCCTGTCGTG 
     3961 CTCAAGGCTG GAAAGTCCAC CTCTGTGCTG CCCAGTTACT ACAGCTGACG AATCTAGAAC 
     4021 ATGATGTCTA TGAAAGACTT ACTAACCTGC AGGAAGGGAT TATCCCAAAG AAAAAAGCAG 
     4081 CAACAGATGA TGATCTCCAC CGAATAAACG AACTGATACA GGGAAATATG CAGAGGTGTA 
     4141 AACTTGTGAT GGATCAAATC AGTGAAGCCA GAGACTCCAT GCTTAAGGTT TTAGATCATA 
     4201 AAGACCGTGT CCTGAAGCTG CTTAACAAGA ACGGGACTGT CAAAAAAGTG TCCAAATTGA 
     4261 AGCGAAAGGA AAAAGTCACG CGTACGCGGC CGCTCGAGCA GAAACTCATC TCAGAAGAGG 
     4321 ATCTGGCAGC AAATGATATC CTGGATTACA AGGATGACGA CGATAAGGTT TAAACGGCCG 
     4381 GCCGCGGTCA TAGCTGTTTC CTGAACAGAT CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA 
     4441 GTGCCTCTCC TGGCCCTGGA AGTTGCCACT CCAGTGCCCA CCAGCCTTGT CCTAATAAAA 
     4501 TTAAGTTGCA TCATTTTGTC TGACTAGGTG TCCTTCTATA ATATTATGGG GTGGAGGGGG 
     4561 GTGGTATGGA GCAAGGGGCA AGTTGGGAAG ACAACCTGTA GGGCCTGCGG GGTCTATTGG 
     4621 GAACCAAGCT GGAGTGCAGT GGCACAATCT TGGCTCACTG CAATCTCCGC CTCCTGGGTT 
     4681 CAAGCGATTC TCCTGCCTCA GCCTCCCGAG TTGTTGGGAT TCCAGGCATG CATGACCAGG 
     4741 CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA CGGGGTTTCA CCATATTGGC CAGGCTGGTC 
     4801 TCCAACTCCT AATCTCAGGT GATCTACCCA CCTTGGCCTC CCAAATTGCT GGGATTACAG 
     4861 GCGTGAACCA CTGCTCCCTT CCCTGTCCTT CTGATTTTAA AATAACTATA CCAGCAGGAG 
     4921 GACGTCCAGA CACAGCATAG GCTACCTGGC CATGCCCAAC CGGTGGGACA TTTGAGTTGC 
     4981 TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG TCCACTCAGT AGATGCCTGT TGAATTGGGT 
     5041 ACGCGGCCAG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA 
     5101 ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG 
     5161 TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA 
     5221 AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT 
     5281 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT 
     5341 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT 
     5401 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC 
     5461 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT 
     5521 ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC 
     5581 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT 
     5641 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA 
     5701 ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA 
     5761 AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA 
     5821 AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT 
     5881 TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAC CTGAGGCTAT 
     5941 GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC GAGCCCTGGG CCTTCACCCG AACTTGGGGG 
     6001 GTGGGGTGGG GAAAAGGAAG AAACGCGGGC GTATTGGCCC CAATGGGGTC TCGGTGGGGT 
     6061 ATCGACAGAG TGCCAGCCCT GGGACCGAAC CCCGCGTTTA TGAACAAACG ACCCAACACC 
     6121 GTGCGTTTTA TTCTGTCTTT TTATTGCCGT CATAGCGCGG GTTCCTTCCG GTATTGTCTC 
     6181 CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG GTGGGCGAAG AACTCCAGCA TGAGATCCCC 
     6241 GCGCTGGAGG ATCATCCAGC CGGCGTCCCG GAAAACGATT CCGAAGCCCA ACCTTTCATA 
     6301 GAAGGCGGCG GTGGAATCGA AATCTCGTGA TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT 
     6361 TTCGAACCCC AGAGTCCCGC TCAGAAGAAC TCGTCAAGAA GGCGATAGAA GGCGATGCGC 
     6421 TGCGAATCGG GAGCGGCGAT ACCGTAAAGC ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA 
     6481 AGCTCTTCAG CAATATCACG GGTAGCCAAC GCTATGTCCT GATAGCGATC CGCCACACCC 
     6541 AGCCGGCCAC AGTCGATGAA TCCAGAAAAG CGGCCATTTT CCACCATGAT ATTCGGCAAG 
     6601 CAGGCATCGC CATGGGTCAC GACGAGATCC TCGCCGTCGG GCATGCTCGC CTTGAGCCTG 
     6661 GCGAACAGTT CGGCTGGCGC GAGCCCCTGA TGCTCTTCGT CCAGATCATC CTGATCGACA 
     6721 AGACCGGCTT CCATCCGAGT ACGTGCTCGC TCGATGCGAT GTTTCGCTTG GTGGTCGAAT 
     6781 GGGCAGGTAG CCGGATCAAG CGTATGCAGC CGCCGCATTG CATCAGCCAT GATGGATACT 
     6841 TTCTCGGCAG GAGCAAGGTG AGATGACAGG AGATCCTGCC CCGGCACTTC GCCCAATAGC 
     6901 AGCCAGTCCC TTCCCGCTTC AGTGACAACG TCGAGCACAG CTGCGCAAGG AACGCCCGTC 
     6961 GTGGCCAGCC ACGATAGCCG CGCTGCCTCG TCTTGCAGTT CATTCAGGGC ACCGGACAGG 
     7021 TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC TGCGCTGACA GCCGGAACAC GGCGGCATCA 
     7081 GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA TAGCCGAATA GCCTCTCCAC CCAAGCGGCC 
     7141 GGAGAACCTG CGTGCAATCC ATCTTGTTCA ATCATGCGAA ACGATCCTCA TCCTGTCTCT 
     7201 TGATCGATCT TTGCAAAAGC CTAGGCCTCC AAAAAAGCCT CCTCACTACT TCTGGAATAG 
     7261 CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC ATAAATAAAA AAAATTAGTC AGCCATGGGG 
     7321 CGGAGAATGG GCGGAACTGG GCGGAGTTAG GGGCGGGATG GGCGGAGTTA GGGGCGGGAC 
     7381 TATGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     7441 GGACTTTCCA CACCTGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     7501 TGGGGAGCCT GGGGACTTTC CACACCCTAA CTGACACACA TTCCACAGCT GGTTCTTTCC 
     7561 GCCTCAGGAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG 
     7621 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT 
     7681 CCCCGAAAAG TGCCACCTGA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG 
     7741 GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC 
     7801 TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC 
     7861 CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT 
     7921 GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG 
     7981 TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG 
     8041 GTCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG 
     8101 CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//