LOCUS       RC229059                8010 bp    DNA     circular  SYN 
DEFINITION  MECOM (Myc-DDK-tagged)-Human MDS1 and EVI1 complex locus (MECOM), transcript variant 5
ACCESSION   NM_001163999
VERSION     NM_001163999.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4167..4258 
                     /label="Myc-DDK"  
     misc_feature    4288..4873 
                     /label="PolyA signal"  
     misc_feature    4345..4364 
                     /label="Restriction Enzyme"  
     misc_feature    5149..5821 
                     /label="ColE1"  
     misc_feature    6251..7045 
                     /label="Kan/Neo"  
     misc_feature    7080..7382 
                     /label="SV40 ori"  
     misc_feature    7468..7945 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4149..4155 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4149 
                     /label="MECOM"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAAGAGCGAA GACTATCCCC ATGAAACTAT GGCGCCGGAT ATCCACGAAG 
     1081 AACGGCAATA TCGCTGCGAA GACTGTGACC AGCTCTTTGA ATCTAAGGCT GAACTAGCAG 
     1141 ATCACCAAAA GTTTCCATGC AGTACTCCTC ACTCAGCATT TTCAATGGTT GAAGAGGACT 
     1201 TTCAGCAAAA ACTCGAAAGC GAGAATGATC TCCAAGAGAT ACACACGATC CAGGAGTGTA 
     1261 AGGAATGTGA CCAAGTTTTT CCTGATTTGC AAAGCCTGGA GAAACACATG CTGTCACATA 
     1321 CTGAAGAGAG GGAATACAAG TGTGATCAGT GTCCCAAGGC ATTTAACTGG AAGTCCAATT 
     1381 TAATTCGCCA CCAGATGTCA CATGACAGTG GAAAGCACTA TGAATGTGAA AACTGTGCCA 
     1441 AGCAGGTTTT CACGGACCCT AGCAACCTTC AGCGGCACAT TCGCTCTCAG CATGTCGGTG 
     1501 CCCGGGCCCA TGCATGCCCG GAGTGTGGCA AAACGTTTGC CACTTCGTCG GGCCTCAAAC 
     1561 AACACAAGCA CATCCACAGC AGTGTGAAGC CCTTTATCTG TGAGGTCTGC CATAAATCCT 
     1621 ATACTCAGTT TTCAAACCTT TGCCGTCATA AGCGCATGCA TGCTGATTGC AGAACCCAAA 
     1681 TCAAGTGCAA AGACTGTGGA CAAATGTTCA GCACTACGTC TTCCTTAAAT AAACACAGGA 
     1741 GGTTTTGTGA GGGCAAGAAC CATTTTGCGG CAGGTGGATT TTTTGGCCAA GGCATTTCAC 
     1801 TTCCTGGAAC CCCAGCTATG GATAAAACGT CCATGGTTAA TATGAGTCAT GCCAACCCGG 
     1861 GCCTTGCTGA CTATTTTGGC GCCAATAGGC ATCCTGCTGG TCTTACCTTT CCAACAGCTC 
     1921 CTGGATTTTC TTTTAGCTTC CCTGGTCTGT TTCCTTCCGG CTTGTACCAC AGGCCTCCTT 
     1981 TGATACCTGC TAGTTCTCCT GTTAAAGGAC TATCAAGTAC TGAACAGACA AACAAAAGTC 
     2041 AAAGTCCCCT CATGACACAT CCTCAGATAC TGCCAGCTAC ACAGGATATT TTGAAGGCAC 
     2101 TATCTAAACA CCCATCTGTA GGGGACAATA AGCCAGTGGA GCTCCAGCCC GAGAGGTCCT 
     2161 CTGAAGAGAG GCCCTTTGAG AAAATCAGTG ACCAGTCAGA GAGTAGTGAC CTTGATGATG 
     2221 TCAGTACACC AAGTGGCAGT GACCTGGAAA CAACCTCGGG CTCTGATCTG GAAAGTGACA 
     2281 TTGAAAGTGA TAAAGAGAAA TTTAAAGAAA ATGGTAAAAT GTTCAAAGAC AAAGTAAGCC 
     2341 CTCTTCAGAA TCTGGCTTCA ATAAATAATA AGAAAGAATA CAGCAATCAT TCCATTTTCT 
     2401 CACCATCTTT AGAGGAGCAG ACTGCGGTGT CAGGAGCTGT GAATGATTCT ATAAAGGCTA 
     2461 TTGCTTCTAT TGCTGAAAAA TACTTTGGTT CAACAGGACT GGTGGGGCTG CAAGACAAAA 
     2521 AAGTTGGAGC TTTACCTTAC CCTTCCATGT TTCCCCTCCC ATTTTTTCCA GCATTCTCTC 
     2581 AATCAATGTA CCCATTTCCT GATAGAGACT TGAGATCGTT ACCTTTGAAA ATGGAACCCC 
     2641 AATCACCAGG TGAAGTAAAG AAACTGCAGA AGGGCAGCTC TGAGTCCCCC TTTGATCTCA 
     2701 CCACTAAGCG AAAGGATGAG AAGCCCTTGA CTCCAGTCCC CTCCAAGCCT CCAGTGACAC 
     2761 CTGCCACAAG CCAAGACCAG CCCCTGGATC TAAGTATGGG CAGTAGGAGT AGAGCCAGTG 
     2821 GGACAAAGCT GACTGAGCCT CGAAAAAACC ACGTGTTTGG GGGAAAAAAA GGAAGCAACG 
     2881 TCGAATCAAG ACCTGCTTCA GATGGTTCCT TGCAGCATGC AAGACCCACT CCTTTCTTTA 
     2941 TGGACCCTAT TTACAGAGTA GAGAAAAGAA AACTAACTGA CCCACTTGAA GCTTTAAAAG 
     3001 AGAAATACTT GAGGCCTTCT CCAGGATTCT TGTTTCACCC ACAAATGTCA GCTATTGAAA 
     3061 ACATGGCAGA AAAGCTAGAG AGCTTCAGTG CCCTGAAACC TGAGGCCAGT GAGCTCTTAC 
     3121 AGTCAGTGCC CTCTATGTTC AACTTCAGGG CGCCTCCCAA TGCCCTGCCA GAGAACCTTC 
     3181 TGCGGAAGGG AAAGGAGCGC TATACCTGCA GATACTGTGG CAAGATTTTT CCAAGGTCTG 
     3241 CAAACCTAAC ACGGCACTTG AGAACCCACA CAGGAGAGCA GCCTTACAGA TGCAAATACT 
     3301 GTGACAGATC ATTTAGCATA TCTTCTAACT TGCAAAGGCA TGTTCGCAAC ATCCACAATA 
     3361 AAGAGAAGCC ATTTAAGTGT CACTTATGTG ATAGGTGTTT TGGTCAACAA ACCAATTTAG 
     3421 ACAGACACCT AAAGAAACAT GAGAATGGGA ACATGTCCGG TACAGCAACA TCGTCGCCTC 
     3481 ATTCTGAACT GGAAAGTACA GGTGCGATTC TGGATGACAA AGAAGATGCT TACTTCACAG 
     3541 AAATTCGAAA TTTCATTGGG AACAGCAACC ATGGCAGCCA ATCTCCCAGG AATGTGGAGG 
     3601 AGAGAATGAA TGGCAGTCAT TTTAAAGATG AAAAGGCTTT GGTGACCAGT CAAAATTCAG 
     3661 ACTTGCTGGA TGATGAAGAA GTTGAAGATG AGGTGTTGTT AGATGAGGAG GATGAAGACA 
     3721 ATGATATTAC TGGAAAAACA GGAAAGGAAC CAGTGACAAG TAATTTACAT GAAGGAAACC 
     3781 CTGAGGATGA CTATGAAGAA ACCAGTGCCC TGGAGATGAG TTGCAAGACA TCCCCAGTGA 
     3841 GGTATAAAGA GGAAGAATAT AAAAGTGGAC TTTCTGCTCT AGATCATATA AGGCACTTCA 
     3901 CAGATAGCCT CAAAATGAGG AAAATGGAAG ATAATCAATA TTCTGAAGCT GAGCTGTCTT 
     3961 CTTTTAGTAC TTCCCATGTG CCAGAGGAAC TTAAGCAGCC GTTACACAGA AAGTCCAAAT 
     4021 CGCAGGCATA TGCTATGATG CTGTCACTGT CTGACAAGGA GTCCCTCCAT TCTACATCCC 
     4081 ACAGTTCTTC CAACGTGTGG CACAGTATGG CCAGGGCTGC GGCGGAATCC AGTGCTATCC 
     4141 AGTCCATAAG CCACGTAACG CGTACGCGGC CGCTCGAGCA GAAACTCATC TCAGAAGAGG 
     4201 ATCTGGCAGC AAATGATATC CTGGATTACA AGGATGACGA CGATAAGGTT TAAACGGCCG 
     4261 GCCGCGGTCA TAGCTGTTTC CTGAACAGAT CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA 
     4321 GTGCCTCTCC TGGCCCTGGA AGTTGCCACT CCAGTGCCCA CCAGCCTTGT CCTAATAAAA 
     4381 TTAAGTTGCA TCATTTTGTC TGACTAGGTG TCCTTCTATA ATATTATGGG GTGGAGGGGG 
     4441 GTGGTATGGA GCAAGGGGCA AGTTGGGAAG ACAACCTGTA GGGCCTGCGG GGTCTATTGG 
     4501 GAACCAAGCT GGAGTGCAGT GGCACAATCT TGGCTCACTG CAATCTCCGC CTCCTGGGTT 
     4561 CAAGCGATTC TCCTGCCTCA GCCTCCCGAG TTGTTGGGAT TCCAGGCATG CATGACCAGG 
     4621 CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA CGGGGTTTCA CCATATTGGC CAGGCTGGTC 
     4681 TCCAACTCCT AATCTCAGGT GATCTACCCA CCTTGGCCTC CCAAATTGCT GGGATTACAG 
     4741 GCGTGAACCA CTGCTCCCTT CCCTGTCCTT CTGATTTTAA AATAACTATA CCAGCAGGAG 
     4801 GACGTCCAGA CACAGCATAG GCTACCTGGC CATGCCCAAC CGGTGGGACA TTTGAGTTGC 
     4861 TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG TCCACTCAGT AGATGCCTGT TGAATTGGGT 
     4921 ACGCGGCCAG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA 
     4981 ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG 
     5041 TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA 
     5101 AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT 
     5161 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT 
     5221 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT 
     5281 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC 
     5341 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT 
     5401 ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC 
     5461 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT 
     5521 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA 
     5581 ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA 
     5641 AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA 
     5701 AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT 
     5761 TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAC CTGAGGCTAT 
     5821 GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC GAGCCCTGGG CCTTCACCCG AACTTGGGGG 
     5881 GTGGGGTGGG GAAAAGGAAG AAACGCGGGC GTATTGGCCC CAATGGGGTC TCGGTGGGGT 
     5941 ATCGACAGAG TGCCAGCCCT GGGACCGAAC CCCGCGTTTA TGAACAAACG ACCCAACACC 
     6001 GTGCGTTTTA TTCTGTCTTT TTATTGCCGT CATAGCGCGG GTTCCTTCCG GTATTGTCTC 
     6061 CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG GTGGGCGAAG AACTCCAGCA TGAGATCCCC 
     6121 GCGCTGGAGG ATCATCCAGC CGGCGTCCCG GAAAACGATT CCGAAGCCCA ACCTTTCATA 
     6181 GAAGGCGGCG GTGGAATCGA AATCTCGTGA TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT 
     6241 TTCGAACCCC AGAGTCCCGC TCAGAAGAAC TCGTCAAGAA GGCGATAGAA GGCGATGCGC 
     6301 TGCGAATCGG GAGCGGCGAT ACCGTAAAGC ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA 
     6361 AGCTCTTCAG CAATATCACG GGTAGCCAAC GCTATGTCCT GATAGCGATC CGCCACACCC 
     6421 AGCCGGCCAC AGTCGATGAA TCCAGAAAAG CGGCCATTTT CCACCATGAT ATTCGGCAAG 
     6481 CAGGCATCGC CATGGGTCAC GACGAGATCC TCGCCGTCGG GCATGCTCGC CTTGAGCCTG 
     6541 GCGAACAGTT CGGCTGGCGC GAGCCCCTGA TGCTCTTCGT CCAGATCATC CTGATCGACA 
     6601 AGACCGGCTT CCATCCGAGT ACGTGCTCGC TCGATGCGAT GTTTCGCTTG GTGGTCGAAT 
     6661 GGGCAGGTAG CCGGATCAAG CGTATGCAGC CGCCGCATTG CATCAGCCAT GATGGATACT 
     6721 TTCTCGGCAG GAGCAAGGTG AGATGACAGG AGATCCTGCC CCGGCACTTC GCCCAATAGC 
     6781 AGCCAGTCCC TTCCCGCTTC AGTGACAACG TCGAGCACAG CTGCGCAAGG AACGCCCGTC 
     6841 GTGGCCAGCC ACGATAGCCG CGCTGCCTCG TCTTGCAGTT CATTCAGGGC ACCGGACAGG 
     6901 TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC TGCGCTGACA GCCGGAACAC GGCGGCATCA 
     6961 GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA TAGCCGAATA GCCTCTCCAC CCAAGCGGCC 
     7021 GGAGAACCTG CGTGCAATCC ATCTTGTTCA ATCATGCGAA ACGATCCTCA TCCTGTCTCT 
     7081 TGATCGATCT TTGCAAAAGC CTAGGCCTCC AAAAAAGCCT CCTCACTACT TCTGGAATAG 
     7141 CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC ATAAATAAAA AAAATTAGTC AGCCATGGGG 
     7201 CGGAGAATGG GCGGAACTGG GCGGAGTTAG GGGCGGGATG GGCGGAGTTA GGGGCGGGAC 
     7261 TATGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     7321 GGACTTTCCA CACCTGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     7381 TGGGGAGCCT GGGGACTTTC CACACCCTAA CTGACACACA TTCCACAGCT GGTTCTTTCC 
     7441 GCCTCAGGAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG 
     7501 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT 
     7561 CCCCGAAAAG TGCCACCTGA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG 
     7621 GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC 
     7681 TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC 
     7741 CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT 
     7801 GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG 
     7861 TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG 
     7921 GTCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG 
     7981 CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//