LOCUS       RC228640                8220 bp    DNA     circular  SYN 
DEFINITION  TSC1 (Myc-DDK-tagged)-Human tuberous sclerosis 1 (TSC1), transcript variant 4
ACCESSION   NM_001162427
VERSION     NM_001162427.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4377..4468 
                     /label="Myc-DDK"  
     misc_feature    4498..5083 
                     /label="PolyA signal"  
     misc_feature    4555..4574 
                     /label="Restriction Enzyme"  
     misc_feature    5359..6031 
                     /label="ColE1"  
     misc_feature    6461..7255 
                     /label="Kan/Neo"  
     misc_feature    7290..7592 
                     /label="SV40 ori"  
     misc_feature    7678..8155 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4359..4365 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4359 
                     /label="TSC1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCCCAACAA GCAAATGTCG GGGAGCTTCT TGCCATGCTG GACTCCCCCA 
     1081 TGCTGGGTGT GCGGGACGAC GTGACAGCTG TCTTTAAAGA GAACCTCAAT TCTGACCGTG 
     1141 GCCCTATGCT TGTAAACACC TTGGTGGATT ATTACCTGGA AACCAGCTCT CAGCCGGCAT 
     1201 TGCACATCCT GACCACCTTG CAAGAGCCAC ATGACAAGAT GGACACTGAC GTCGTTGTCC 
     1261 TCACAACAGG CGTCTTGGTG TTGATAACCA TGCTACCAAT GATTCCACAG TCTGGGAAAC 
     1321 AGCATCTTCT TGATTTCTTT GACATTTTTG GCCGTCTGTC ATCATGGTGC CTGAAGAAAC 
     1381 CAGGCCACGT GGCGGAAGTC TATCTCGTCC ATCTCCATGC CAGTGTGTAC GCACTCTTTC 
     1441 ATCGCCTTTA TGGAATGTAC CCTTGCAACT TCGTCTCCTT TTTGCGTTCT CATTACAGTA 
     1501 TGAAAGAAAA CCTGGAGACT TTTGAAGAAG TGGTCAAGCC AATGATGGAG CATGTGCGAA 
     1561 TTCATCCGGA ATTAGTGACT GGATCCAAGG ACCATGAACT GGACCCTCGA AGGTGGAAGA 
     1621 GATTAGAAAC TCATGATGTT GTGATCGAGT GTGCCAAAAT CTCTCTGGAT CCCACAGAAG 
     1681 CCTCATATGA AGATGGCTAT TCTGTGTCTC ACCAAATCTC AGCCCGCTTT CCTCATCGTT 
     1741 CAGCCGATGT CACCACCAGC CCTTATGCTG ACACACAGAA TAGCTATGGG TGTGCTACTT 
     1801 CTACCCCTTA CTCCACGTCT CGGCTGATGT TGTTAAATAT GCCAGGGCAG CTACCTCAGA 
     1861 CTCTGAGTTC CCCATCGACA CGGCTGATAA CTGAACCACC ACAAGCTACT CTTTGGAGCC 
     1921 CATCTATGGT TTGTGGTATG ACCACTCCTC CAACTTCTCC TGGAAATGTC CCACCTGATC 
     1981 TGTCACACCC TTACAGTAAA GTCTTTGGTA CAACTGCAGG TGGAAAAGGA ACTCCTCTGG 
     2041 GAACCCCAGC AACCTCTCCT CCTCCAGCCC CACTCTGTCA TTCGGATGAC TACGTGCACA 
     2101 TTTCACTCCC CCAGGCCACA GTCACACCCC CCAGGAAGGA AGAGAGAATG GATTCTGCAA 
     2161 GACCATGTCT ACACAGACAA CACCATCTTC TGAATGACAG AGGATCAGAA GAGCCACCTG 
     2221 GCAGCAAAGG TTCTGTCACT CTAAGTGATC TTCCAGGGTT TTTAGGTGAT CTGGCCTCTG 
     2281 AAGAAGATAG TATTGAAAAA GATAAAGAAG AAGCTGCAAT ATCTAGAGAA CTTTCTGAGA 
     2341 TCACCACAGC AGAGGCAGAG CCTGTGGTTC CTCGAGGAGG CTTTGACTCT CCCTTTTACC 
     2401 GAGACAGTCT CCCAGGTTCT CAGCGGAAGA CCCACTCGGC AGCCTCCAGT TCTCAGGGCG 
     2461 CCAGCGTGAA CCCTGAGCCT TTACACTCCT CCCTGGACAA GCTTGGGCCT GACACACCAA 
     2521 AGCAAGCCTT TACTCCCATA GACCTGCCCT GCGGCAGTGC TGATGAAAGC CCTGCGGGAG 
     2581 ACAGGGAATG CCAGACTTCT TTGGAGACCA GTATCTTCAC TCCCAGTCCT TGTAAAATTC 
     2641 CACCTCCGAC GAGAGTGGGC TTTGGAAGCG GGCAGCCTCC CCCGTATGAT CATCTTTTTG 
     2701 AGGTGGCATT GCCAAAGACA GCCCATCATT TTGTCATCAG GAAGACTGAG GAGCTGTTAA 
     2761 AGAAAGCAAA AGGAAACACA GAGGAAGATG GTGTGCCCTC TACCTCCCCA ATGGAAGTGC 
     2821 TGGACAGACT GATACAGCAG GGAGCAGACG CGCACAGCAA GGAGCTGAAC AAGTTGCCTT 
     2881 TACCCAGCAA GTCTGTCGAC TGGACCCACT TTGGAGGCTC TCCTCCTTCA GATGAGATCC 
     2941 GCACCCTCCG AGACCAGTTG CTTTTACTGC ACAACCAGTT ACTCTATGAG CGTTTTAAGA 
     3001 GGCAGCAGCA TGCCCTCCGG AACAGGCGGC TCCTCCGCAA GGTGATCAAA GCAGCAGCTC 
     3061 TGGAGGAACA TAATGCTGCC ATGAAAGATC AGTTGAAGTT ACAAGAGAAG GACATCCAGA 
     3121 TGTGGAAGGT TAGTCTGCAG AAAGAACAAG CTAGATACAA TCAGCTCCAG GAGCAGCGTG 
     3181 ACACTATGGT AACCAAGCTC CACAGCCAGA TCAGACAGCT GCAGCATGAC CGAGAGGAAT 
     3241 TCTACAACCA GAGCCAGGAA TTACAGACGA AGCTGGAGGA CTGCAGGAAC ATGATTGCGG 
     3301 AGCTGCGGAT AGAACTGAAG AAGGCCAACA ACAAGGTGTG TCACACTGAG CTGCTGCTCA 
     3361 GTCAGGTTTC CCAAAAGCTC TCAAACAGTG AGTCGGTCCA GCAGCAGATG GAGTTCTTGA 
     3421 ACAGGCAGCT GTTGGTTCTT GGGGAGGTCA ACGAGCTCTA TTTGGAACAA CTGCAGAACA 
     3481 AGCACTCAGA TACCACAAAG GAAGTAGAAA TGATGAAAGC CGCCTATCGG AAAGAGCTAG 
     3541 AAAAAAACAG AAGCCATGTT CTCCAGCAGA CTCAGAGGCT TGATACCTCC CAAAAACGGA 
     3601 TTTTGGAACT GGAATCTCAC CTGGCCAAGA AAGACCACCT TCTTTTGGAA CAGAAGAAAT 
     3661 ATCTAGAGGA TGTCAAACTC CAGGCAAGAG GACAGCTGCA GGCCGCAGAG AGCAGGTATG 
     3721 AGGCTCAGAA AAGGATAACC CAGGTGTTTG AATTGGAGAT CTTAGATTTA TATGGCAGGT 
     3781 TGGAGAAAGA TGGCCTCCTG AAAAAACTTG AAGAAGAAAA AGCAGAAGCA GCTGAAGCAG 
     3841 CAGAAGAAAG GCTTGACTGT TGTAATGACG GGTGCTCAGA TTCCATGGTA GGGCACAATG 
     3901 AAGAGGCATC TGGCCACAAC GGTGAGACCA AGACCCCCAG GCCCAGCAGC GCCCGGGGCA 
     3961 GTAGTGGAAG CAGAGGTGGT GGAGGCAGCA GCAGCAGCAG CAGCGAGCTT TCTACCCCAG 
     4021 AGAAACCCCC ACACCAGAGG GCAGGCCCAT TCAGCAGTCG GTGGGAGACG ACTATGGGAG 
     4081 AAGCGTCTGC CAGCATCCCC ACCACTGTGG GCTCACTTCC CAGTTCAAAA AGCTTCCTGG 
     4141 GTATGAAGGC TCGAGAGTTA TTTCGTAATA AGAGCGAGAG CCAGTGTGAT GAGGACGGCA 
     4201 TGACCAGTAG CCTTTCTGAG AGCCTAAAGA CAGAACTGGG CAAAGACTTG GGTGTGGAAG 
     4261 CCAAGATTCC CCTGAACCTA GATGGCCCTC ACCCGTCTCC CCCGACCCCG GACAGTGTTG 
     4321 GACAGCTACA TATCATGGAC TACAATGAGA CTCATCATGA ACACAGCACG CGTACGCGGC 
     4381 CGCTCGAGCA GAAACTCATC TCAGAAGAGG ATCTGGCAGC AAATGATATC CTGGATTACA 
     4441 AGGATGACGA CGATAAGGTT TAAACGGCCG GCCGCGGTCA TAGCTGTTTC CTGAACAGAT 
     4501 CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA GTGCCTCTCC TGGCCCTGGA AGTTGCCACT 
     4561 CCAGTGCCCA CCAGCCTTGT CCTAATAAAA TTAAGTTGCA TCATTTTGTC TGACTAGGTG 
     4621 TCCTTCTATA ATATTATGGG GTGGAGGGGG GTGGTATGGA GCAAGGGGCA AGTTGGGAAG 
     4681 ACAACCTGTA GGGCCTGCGG GGTCTATTGG GAACCAAGCT GGAGTGCAGT GGCACAATCT 
     4741 TGGCTCACTG CAATCTCCGC CTCCTGGGTT CAAGCGATTC TCCTGCCTCA GCCTCCCGAG 
     4801 TTGTTGGGAT TCCAGGCATG CATGACCAGG CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA 
     4861 CGGGGTTTCA CCATATTGGC CAGGCTGGTC TCCAACTCCT AATCTCAGGT GATCTACCCA 
     4921 CCTTGGCCTC CCAAATTGCT GGGATTACAG GCGTGAACCA CTGCTCCCTT CCCTGTCCTT 
     4981 CTGATTTTAA AATAACTATA CCAGCAGGAG GACGTCCAGA CACAGCATAG GCTACCTGGC 
     5041 CATGCCCAAC CGGTGGGACA TTTGAGTTGC TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG 
     5101 TCCACTCAGT AGATGCCTGT TGAATTGGGT ACGCGGCCAG CGGCGAGCGG TATCAGCTCA 
     5161 CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG 
     5221 AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA 
     5281 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA 
     5341 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC 
     5401 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC 
     5461 GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT 
     5521 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG 
     5581 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG 
     5641 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA 
     5701 CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG 
     5761 AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT 
     5821 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT 
     5881 TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG 
     5941 ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT 
     6001 CTAAAGTATA TATGAGTAAC CTGAGGCTAT GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC 
     6061 GAGCCCTGGG CCTTCACCCG AACTTGGGGG GTGGGGTGGG GAAAAGGAAG AAACGCGGGC 
     6121 GTATTGGCCC CAATGGGGTC TCGGTGGGGT ATCGACAGAG TGCCAGCCCT GGGACCGAAC 
     6181 CCCGCGTTTA TGAACAAACG ACCCAACACC GTGCGTTTTA TTCTGTCTTT TTATTGCCGT 
     6241 CATAGCGCGG GTTCCTTCCG GTATTGTCTC CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG 
     6301 GTGGGCGAAG AACTCCAGCA TGAGATCCCC GCGCTGGAGG ATCATCCAGC CGGCGTCCCG 
     6361 GAAAACGATT CCGAAGCCCA ACCTTTCATA GAAGGCGGCG GTGGAATCGA AATCTCGTGA 
     6421 TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT TTCGAACCCC AGAGTCCCGC TCAGAAGAAC 
     6481 TCGTCAAGAA GGCGATAGAA GGCGATGCGC TGCGAATCGG GAGCGGCGAT ACCGTAAAGC 
     6541 ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA AGCTCTTCAG CAATATCACG GGTAGCCAAC 
     6601 GCTATGTCCT GATAGCGATC CGCCACACCC AGCCGGCCAC AGTCGATGAA TCCAGAAAAG 
     6661 CGGCCATTTT CCACCATGAT ATTCGGCAAG CAGGCATCGC CATGGGTCAC GACGAGATCC 
     6721 TCGCCGTCGG GCATGCTCGC CTTGAGCCTG GCGAACAGTT CGGCTGGCGC GAGCCCCTGA 
     6781 TGCTCTTCGT CCAGATCATC CTGATCGACA AGACCGGCTT CCATCCGAGT ACGTGCTCGC 
     6841 TCGATGCGAT GTTTCGCTTG GTGGTCGAAT GGGCAGGTAG CCGGATCAAG CGTATGCAGC 
     6901 CGCCGCATTG CATCAGCCAT GATGGATACT TTCTCGGCAG GAGCAAGGTG AGATGACAGG 
     6961 AGATCCTGCC CCGGCACTTC GCCCAATAGC AGCCAGTCCC TTCCCGCTTC AGTGACAACG 
     7021 TCGAGCACAG CTGCGCAAGG AACGCCCGTC GTGGCCAGCC ACGATAGCCG CGCTGCCTCG 
     7081 TCTTGCAGTT CATTCAGGGC ACCGGACAGG TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC 
     7141 TGCGCTGACA GCCGGAACAC GGCGGCATCA GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA 
     7201 TAGCCGAATA GCCTCTCCAC CCAAGCGGCC GGAGAACCTG CGTGCAATCC ATCTTGTTCA 
     7261 ATCATGCGAA ACGATCCTCA TCCTGTCTCT TGATCGATCT TTGCAAAAGC CTAGGCCTCC 
     7321 AAAAAAGCCT CCTCACTACT TCTGGAATAG CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC 
     7381 ATAAATAAAA AAAATTAGTC AGCCATGGGG CGGAGAATGG GCGGAACTGG GCGGAGTTAG 
     7441 GGGCGGGATG GGCGGAGTTA GGGGCGGGAC TATGGTTGCT GACTAATTGA GATGCATGCT 
     7501 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCTGGTTG CTGACTAATT 
     7561 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCCTAA 
     7621 CTGACACACA TTCCACAGCT GGTTCTTTCC GCCTCAGGAC TCTTCCTTTT TCAATATTAT 
     7681 TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA 
     7741 AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA CGCGCCCTGT 
     7801 AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC 
     7861 AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC 
     7921 TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG 
     7981 CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA 
     8041 TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC 
     8101 CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA AGGGATTTTG 
     8161 CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//