LOCUS       RC227884                8304 bp    DNA     circular  SYN 
DEFINITION  ITGA7 (Myc-DDK-tagged)-Human integrin, alpha 7 (ITGA7), transcript variant 1
ACCESSION   NM_001144996
VERSION     NM_001144996.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4461..4552 
                     /label="Myc-DDK"  
     misc_feature    4582..5167 
                     /label="PolyA signal"  
     misc_feature    4639..4658 
                     /label="Restriction Enzyme"  
     misc_feature    5443..6115 
                     /label="ColE1"  
     misc_feature    6545..7339 
                     /label="Kan/Neo"  
     misc_feature    7374..7676 
                     /label="SV40 ori"  
     misc_feature    7762..8239 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4443..4449 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4443 
                     /label="ITGA7"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCCGGGGCT CGGAGCCGCG ACCCTTGGGG GGCCTCCGGG ATTTGCTACC 
     1081 TTTTTGGCTC CCTGCTCGTC GAACTGCTCT TCTCACGGGC TGTCGCCTTC AATCTGGACG 
     1141 TGATGGGTGC CTTGCGCAAG GAGGGCGAGC CAGGCAGCCT CTTCGGCTTC TCTGTGGCCC 
     1201 TGCACCGGCA GTTGCAGCCC CGACCCCAGA GCTGGCTGCT GGTGGGTGCT CCCCAGGCCC 
     1261 TGGCTCTTCC TGGGCAGCAG GCGAATCGCA CTGGAGGCCT CTTCGCTTGC CCGTTGAGCC 
     1321 TGGAGGAGAC TGACTGCTAC AGAGTGGACA TCGACCAGGG AGCTGATATG CAAAAGGAAA 
     1381 GCAAGGAGAA CCAGTGGTTG GGAGTCAGTG TTCGGAGCCA GGGGCCTGGG GGCAAGATTG 
     1441 TTACCTGTGC ACACCGATAT GAGGCAAGGC AGCGAGTGGA CCAGATCCTG GAGACGCGGG 
     1501 ATATGATTGG TCGCTGCTTT GTGCTCAGCC AGGACCTGGC CATCCGGGAT GAGTTGGATG 
     1561 GTGGGGAATG GAAGTTCTGT GAGGGACGCC CCCAAGGCCA TGAACAATTT GGGTTCTGCC 
     1621 AGCAGGGCAC AGCTGCCGCC TTCTCCCCTG ATAGCCACTA CCTCCTCTTT GGGGCCCCAG 
     1681 GAACCTATAA TTGGAAGGGC ACGGCCAGGG TGGAGCTCTG TGCACAGGGC TCAGCGGACC 
     1741 TGGCACACCT GGACGACGGT CCCTACGAGG CGGGGGGAGA GAAGGAGCAG GACCCCCGCC 
     1801 TCATCCCGGT CCCTGCCAAC AGCTACTTTG GCTTCTCTAT TGACTCGGGG AAAGGTCTGG 
     1861 TGCGTGCAGA AGAGCTGAGC TTTGTGGCTG GAGCCCCCCG CGCCAACCAC AAGGGTGCTG 
     1921 TGGTCATCCT GCGCAAGGAC AGCGCCAGTC GCCTGGTGCC CGAGGTTATG CTGTCTGGGG 
     1981 AGCGCCTGAC CTCCGGCTTT GGCTACTCAC TGGCTGTGGC TGACCTCAAC AGTGATGGCT 
     2041 GGCCAGACCT GATAGTGGGT GCCCCCTACT TCTTTGAGCG CCAAGAAGAG CTGGGGGGTG 
     2101 CTGTGTATGT GTACTTGAAC CAGGGGGGTC ACTGGGCTGG GATCTCCCCT CTCCGGCTCT 
     2161 GCGGCTCCCC TGACTCCATG TTCGGGATCA GCCTGGCTGT CCTGGGGGAC CTCAACCAAG 
     2221 ATGGCTTTCC AGATATTGCA GTGGGTGCCC CCTTTGATGG TGATGGGAAA GTCTTCATCT 
     2281 ACCATGGGAG CAGCCTGGGG GTTGTCGCCA AACCTTCACA GGTGCTGGAG GGCGAGGCTG 
     2341 TGGGCATCAA GAGCTTCGGC TACTCCCTGT CAGGCAGCTT GGATATGGAT GGGAACCAAT 
     2401 ACCCTGACCT GCTGGTGGGC TCCCTGGCTG ACACCGCAGT GCTCTTCAGG GCCAGACCCA 
     2461 TCCTCCATGT CTCCCATGAG GTCTCTATTG CTCCACGAAG CATCGACCTG GAGCAGCCCA 
     2521 ACTGTGCTGG CGGCCACTCG GTCTGTGTGG ACCTAAGGGT CTGTTTCAGC TACATTGCAG 
     2581 TCCCCAGCAG CTATAGCCCT ACTGTGGCCC TGGACTATGT GTTAGATGCG GACACAGACC 
     2641 GGAGGCTCCG GGGCCAGGTT CCCCGTGTGA CGTTCCTGAG CCGTAACCTG GAAGAACCCA 
     2701 AGCACCAGGC CTCGGGCACC GTGTGGCTGA AGCACCAGCA TGACCGAGTC TGTGGAGACG 
     2761 CCATGTTCCA GCTCCAGGAA AATGTCAAAG ACAAGCTTCG GGCCATTGTA GTGACCTTGT 
     2821 CCTACAGTCT CCAGACCCCT CGGCTCCGGC GACAGGCTCC TGGCCAGGGG CTGCCTCCAG 
     2881 TGGCCCCCAT CCTCAATGCC CACCAGCCCA GCACCCAGCG GGCAGAGATC CACTTCCTGA 
     2941 AGCAAGGCTG TGGTGAAGAC AAGATCTGCC AGAGCAATCT GCAGCTGGTC CGCGCCCGCT 
     3001 TCTGTACCCG GGTCAGCGAC ACGGAATTCC AACCTCTGCC CATGGATGTG GATGGAACAA 
     3061 CAGCCCTGTT TGCACTGAGT GGGCAGCCAG TCATTGGCCT GGAGCTGATG GTCACCAACC 
     3121 TGCCATCGGA CCCAGCCCAG CCCCAGGCTG ATGGGGATGA TGCCCATGAA GCCCAGCTCC 
     3181 TGGTCATGCT TCCTGACTCA CTGCACTACT CAGGGGTCCG GGCCCTGGAC CCTGCGGAGA 
     3241 AGCCACTCTG CCTGTCCAAT GAGAATGCCT CCCATGTTGA GTGTGAGCTG GGGAACCCCA 
     3301 TGAAGAGAGG TGCCCAGGTC ACCTTCTACC TCATCCTTAG CACCTCCGGG ATCAGCATTG 
     3361 AGACCACGGA ACTGGAGGTA GAGCTGCTGT TGGCCACGAT CAGTGAGCAG GAGCTGCATC 
     3421 CAGTCTCTGC ACGAGCCCGT GTCTTCATTG AGCTGCCACT GTCCATTGCA GGAATGGCCA 
     3481 TTCCCCAGCA ACTCTTCTTC TCTGGTGTGG TGAGGGGCGA GAGAGCCATG CAGTCTGAGC 
     3541 GGGATGTGGG CAGCAAGGTC AAGTATGAGG TCACGGTTTC CAACCAAGGC CAGTCGCTCA 
     3601 GAACCCTGGG CTCTGCCTTC CTCAACATCA TGTGGCCTCA TGAGATTGCC AATGGGAAGT 
     3661 GGTTGCTGTA CCCAATGCAG GTTGAGCTGG AGGGCGGGCA GGGGCCTGGG CAGAAAGGGC 
     3721 TTTGCTCTCC CAGGCCCAAC ATCCTCCACC TGGATGTGGA CAGTAGGGAT AGGAGGCGGC 
     3781 GGGAGCTGGA GCCACCTGAG CAGCAGGAGC CTGGTGAGCG GCAGGAGCCC AGCATGTCCT 
     3841 GGTGGCCAGT GTCCTCTGCT GAGAAGAAGA AAAACATCAC CCTGGACTGC GCCCGGGGCA 
     3901 CGGCCAACTG TGTGGTGTTC AGCTGCCCAC TCTACAGCTT TGACCGCGCG GCTGTGCTGC 
     3961 ATGTCTGGGG CCGTCTCTGG AACAGCACCT TTCTGGAGGA GTACTCAGCT GTGAAGTCCC 
     4021 TGGAAGTGAT TGTCCGGGCC AACATCACAG TGAAGTCCTC CATAAAGAAC TTGATGCTCC 
     4081 GAGATGCCTC CACAGTGATC CCAGTGATGG TATACTTGGA CCCCATGGCT GTGGTGGCAG 
     4141 AAGGAGTGCC CTGGTGGGTC ATCCTCCTGG CTGTACTGGC TGGGCTGCTG GTGCTAGCAC 
     4201 TGCTGGTGCT GCTCCTGTGG AAGATGGGAT TCTTCAAACG GGCGAAGCAC CCCGAGGCCA 
     4261 CCGTGCCCCA GTACCATGCG GTGAAGATTC CTCGGGAAGA CCGACAGCAG TTCAAGGAGG 
     4321 AGAAGACGGG CACCATCCTG AGGAACAACT GGGGCAGCCC CCGGCGGGAG GGCCCGGATG 
     4381 CACACCCCAT CCTGGCTGCT GACGGGCATC CCGAGCTGGG CCCCGATGGG CATCCAGGGC 
     4441 CAGGCACCGC CACGCGTACG CGGCCGCTCG AGCAGAAACT CATCTCAGAA GAGGATCTGG 
     4501 CAGCAAATGA TATCCTGGAT TACAAGGATG ACGACGATAA GGTTTAAACG GCCGGCCGCG 
     4561 GTCATAGCTG TTTCCTGAAC AGATCCCGGG TGGCATCCCT GTGACCCCTC CCCAGTGCCT 
     4621 CTCCTGGCCC TGGAAGTTGC CACTCCAGTG CCCACCAGCC TTGTCCTAAT AAAATTAAGT 
     4681 TGCATCATTT TGTCTGACTA GGTGTCCTTC TATAATATTA TGGGGTGGAG GGGGGTGGTA 
     4741 TGGAGCAAGG GGCAAGTTGG GAAGACAACC TGTAGGGCCT GCGGGGTCTA TTGGGAACCA 
     4801 AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT CCGCCTCCTG GGTTCAAGCG 
     4861 ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG CATGCATGAC CAGGCTCAGC 
     4921 TAATTTTTGT TTTTTTGGTA GAGACGGGGT TTCACCATAT TGGCCAGGCT GGTCTCCAAC 
     4981 TCCTAATCTC AGGTGATCTA CCCACCTTGG CCTCCCAAAT TGCTGGGATT ACAGGCGTGA 
     5041 ACCACTGCTC CCTTCCCTGT CCTTCTGATT TTAAAATAAC TATACCAGCA GGAGGACGTC 
     5101 CAGACACAGC ATAGGCTACC TGGCCATGCC CAACCGGTGG GACATTTGAG TTGCTTGCTT 
     5161 GGCACTGTCC TCTCATGCGT TGGGTCCACT CAGTAGATGC CTGTTGAATT GGGTACGCGG 
     5221 CCAGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG 
     5281 GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA 
     5341 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG 
     5401 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC 
     5461 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC 
     5521 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC 
     5581 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG 
     5641 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC 
     5701 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA 
     5761 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC 
     5821 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC 
     5881 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG 
     5941 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC 
     6001 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA 
     6061 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAACCTGAGG CTATGGCAGG 
     6121 GCCTGCCGCC CCGACGTTGG CTGCGAGCCC TGGGCCTTCA CCCGAACTTG GGGGGTGGGG 
     6181 TGGGGAAAAG GAAGAAACGC GGGCGTATTG GCCCCAATGG GGTCTCGGTG GGGTATCGAC 
     6241 AGAGTGCCAG CCCTGGGACC GAACCCCGCG TTTATGAACA AACGACCCAA CACCGTGCGT 
     6301 TTTATTCTGT CTTTTTATTG CCGTCATAGC GCGGGTTCCT TCCGGTATTG TCTCCTTCCG 
     6361 TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC GAAGAACTCC AGCATGAGAT CCCCGCGCTG 
     6421 GAGGATCATC CAGCCGGCGT CCCGGAAAAC GATTCCGAAG CCCAACCTTT CATAGAAGGC 
     6481 GGCGGTGGAA TCGAAATCTC GTGATGGCAG GTTGGGCGTC GCTTGGTCGG TCATTTCGAA 
     6541 CCCCAGAGTC CCGCTCAGAA GAACTCGTCA AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA 
     6601 TCGGGAGCGG CGATACCGTA AAGCACGAGG AAGCGGTCAG CCCATTCGCC GCCAAGCTCT 
     6661 TCAGCAATAT CACGGGTAGC CAACGCTATG TCCTGATAGC GATCCGCCAC ACCCAGCCGG 
     6721 CCACAGTCGA TGAATCCAGA AAAGCGGCCA TTTTCCACCA TGATATTCGG CAAGCAGGCA 
     6781 TCGCCATGGG TCACGACGAG ATCCTCGCCG TCGGGCATGC TCGCCTTGAG CCTGGCGAAC 
     6841 AGTTCGGCTG GCGCGAGCCC CTGATGCTCT TCGTCCAGAT CATCCTGATC GACAAGACCG 
     6901 GCTTCCATCC GAGTACGTGC TCGCTCGATG CGATGTTTCG CTTGGTGGTC GAATGGGCAG 
     6961 GTAGCCGGAT CAAGCGTATG CAGCCGCCGC ATTGCATCAG CCATGATGGA TACTTTCTCG 
     7021 GCAGGAGCAA GGTGAGATGA CAGGAGATCC TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG 
     7081 TCCCTTCCCG CTTCAGTGAC AACGTCGAGC ACAGCTGCGC AAGGAACGCC CGTCGTGGCC 
     7141 AGCCACGATA GCCGCGCTGC CTCGTCTTGC AGTTCATTCA GGGCACCGGA CAGGTCGGTC 
     7201 TTGACAAAAA GAACCGGGCG CCCCTGCGCT GACAGCCGGA ACACGGCGGC ATCAGAGCAG 
     7261 CCGATTGTCT GTTGTGCCCA GTCATAGCCG AATAGCCTCT CCACCCAAGC GGCCGGAGAA 
     7321 CCTGCGTGCA ATCCATCTTG TTCAATCATG CGAAACGATC CTCATCCTGT CTCTTGATCG 
     7381 ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA GCCTCCTCAC TACTTCTGGA ATAGCTCAGA 
     7441 GGCCGAGGCG GCCTCGGCCT CTGCATAAAT AAAAAAAATT AGTCAGCCAT GGGGCGGAGA 
     7501 ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG GATGGGCGGA GTTAGGGGCG GGACTATGGT 
     7561 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     7621 TCCACACCTG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     7681 GCCTGGGGAC TTTCCACACC CTAACTGACA CACATTCCAC AGCTGGTTCT TTCCGCCTCA 
     7741 GGACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA 
     7801 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA 
     7861 AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG 
     7921 CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT 
     7981 TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA 
     8041 GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT 
     8101 TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG 
     8161 TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT 
     8221 TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT 
     8281 TAACAAAAAT TTAACGCGAA TTTT
//