LOCUS       RC227818L3                9395 bp    DNA     circular  SYN 
DEFINITION  PPP1R21 (Myc-DDK-tagged)-Human KLRAQ motif containing 1 (KLRAQ1), transcript variant 1
ACCESSION   NM_001135629
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    7686..7866 
                     /label="3-LTR"  
     misc_feature    833..1013 
                     /label="5-LTR"  
     misc_feature    8594..9253 
                     /label="CAT(Cam-r)"  
     misc_feature    2620..3234 
                     /label="CMV"  
     misc_feature    7914..8533 
                     /label="ColE1"  
     misc_feature    5726..5749 
                     /label="DDK"  
     misc_feature    6561..6581 
                     /label="LR50"  
     misc_feature    5678..5749 
                     /label="MYC"  
     misc_feature    5759..5824 
                     /label="P2A"  
     misc_feature    5825..6424 
                     /label="Puro"  
     misc_feature    3252..3271 
                     /label="T7"  
     misc_feature    3221..3237 
                     /label="V2"  
     misc_feature    6570..7161 
                     /label="WPRE"  
     misc_feature    3320..3328 
                     /label="Restriction Enzyme"  
     misc_feature    5660..5666 
                     /label="Restriction Enzyme"  
     misc_feature    3320..5660 
                     /label="PPP1R21"  
ORIGIN                      
        1 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG 
       61 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT 
      121 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC 
      181 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT CGCGTTGACA 
      241 TTGATTATTG ACTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA 
      301 TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA 
      361 CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT 
      421 CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT 
      481 GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA 
      541 TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT 
      601 CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC AATGGGCGTG GATAGCGGTT 
      661 TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT TGTTTTGGCA 
      721 CCAAAATCAA CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA CGCAAATGGG 
      781 CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGCGCG TTTTGCCTGT ACTGGGTCTC 
      841 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 
      901 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 
      961 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 
     1021 CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG 
     1081 GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT 
     1141 TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG 
     1201 GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA 
     1261 ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT 
     1321 GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC 
     1381 AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA 
     1441 AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA 
     1501 AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT 
     1561 ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA 
     1621 GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA 
     1681 ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA 
     1741 ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT 
     1801 TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG 
     1861 CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG 
     1921 ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG 
     1981 AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA 
     2041 ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA 
     2101 AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC 
     2161 ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT 
     2221 TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA 
     2281 TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA 
     2341 GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCGGCACTG 
     2401 CGTGCGCCAA TTCTGCAGAC AAATGGCAGT ATTCATCCAC AATTTTAAAA GAAAAGGGGG 
     2461 GATTGGGGGG TACAGTGCAG GGGAAAGAAT AGTAGACATA ATAGCAACAG ACATACAAAC 
     2521 TAAAGAATTA CAAAAACAAA TTACAAAAAT TCAAAATTTT CGGGTTTATT ACAGGGACAG 
     2581 CAGAGATCCA GTTTGGTTAG TACCGGGCCC GCTCTAGACA TGTCCAATAT GACCGCCATG 
     2641 TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG 
     2701 CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC 
     2761 CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG 
     2821 GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA 
     2881 TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC 
     2941 CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT 
     3001 ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA 
     3061 GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT 
     3121 TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA 
     3181 AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG 
     3241 TCAGAATTTT GTAATACGAC TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT 
     3301 ACCGAGGAGA TCTGCCGCCG CGATCGCCAT GGCCTCGGCT GAGTTGCAGG GGAAGTACCA 
     3361 GAAGCTGGCT CAGGAGTACT CGAAGCTTCG GGCTCAGAAT CAGGTTCTGA AAAAAGGTGT 
     3421 TGTGGATGAA CAAGCAAATT CTGCAGCTTT AAAGGAGCAA CTGAAAATGA AGGATCAGTC 
     3481 ATTGAGAAAA CTACAACAGG AAATGGACAG TTTGACATTT CGAAATCTGC AGCTTGCCAA 
     3541 GAGGGTAGAA CTACTTCAAG ATGAACTAGC TCTAAGTGAA CCACGAGGCA AGAAAAACAA 
     3601 GAAAAGTGGA GAATCTTCTT CTCAGTTGAG TCAAGAGCAG AAGAGTGTCT TTGATGAAGA 
     3661 TCTGCAAAAG AAGATAGAAG AGAATGAACG GTTGCATATA CAATTTTTTG AAGCTGATGA 
     3721 GCAGCACAAG CATGTGGAAG CAGAGCTGAG GAGTCGACTG GCCACTCTGG AGACAGAAGC 
     3781 AGCCCAGCAC CAAGCTGTGG TTGACGGTCT CACCCGGAAG TACATGGAAA CCATTGAGAA 
     3841 GCTGCAGAAC GACAAGGCTA AACTAGAAGT GAAATCTCAG ACTCTAGAAA AGGAAGCCAA 
     3901 GGAATGTCGA CTTCGAACGG AAGAATGTCA ATTACAGTTA AAGACTCTTC ATGAAGATTT 
     3961 GTCAGGTAGA TTAGAGGAAT CCTTATCAAT CATCAATGAA AAAGTACCTT TTAATGATAC 
     4021 AAAATATAGT CAGTACAACG CTCTGAACGT TCCACTCCAC AATAGGAGAC ACCAGCTGAA 
     4081 GATGCGAGAT ATTGCTGGGC AGGCCCTGGC TTTTGTTCAG GATCTTGTGA CGGCTCTTCT 
     4141 AAACTTTCAT ACCTACACAG AACAGAGGAT TCAAATTTTT CCTGTTGATT CTGCCATTGA 
     4201 CACTATATCT CCATTGAATC AGAAGTTCTC ACAATACCTT CATGAAAATG CGTCCTATGT 
     4261 CCGCCCTCTT GAGGAAGGAA TGCTTCATTT ATTTGAAAGT ATCACTGAGG ATACTGTGAC 
     4321 TGTCTTGGAG ACAACTGTGA AATTGAAAAC TTTTTCAGAA CACTTAACCT CCTACATATG 
     4381 TTTTCTTAGG AAGATTCTTC CCTATCAGTT AAAAAGTTTA GAAGAAGAAT GTGAATCCTC 
     4441 TCTTTGCACA TCTGCGTTAA GAGCCAGGAA TCTAGAGCTG TCCCAGGACA TGAAAAAAAT 
     4501 GACAGCTGTG TTTGAGAAGC TGCAGACTTA CATAGCTCTT CTTGCCTTGC CAAGTACAGA 
     4561 GCCAGATGGA CTCCTTCGGA CAAACTACAG TTCTGTGTTA ACAAATGTTG GTGCTGCTCT 
     4621 GCATGGATTT CATGACGTTA TGAAAGATAT TTCCAAACAT TATAGTCAAA AAGCTGCAAT 
     4681 AGAGCATGAA CTTCCAACAG CAACACAGAA GCTGATAACA ACTAATGACT GTATCCTGTC 
     4741 ATCAGTAGTG GCATTAACAA ATGGAGCAGG AAAGATTGCA TCCTTCTTCA GCAACAATTT 
     4801 GGACTACTTC ATTGCTTCAC TGAGCTATGG ACCTAAGGCA GCGAGTGGAT TCATTAGTCC 
     4861 TCTTTCAGCT GAATGCATGC TACAGTATAA GAAAAAAGCT GCTGCCTATA TGAAGTCTTT 
     4921 GAGAAAGCCC CTCTTGGAGT CTGTGCCTTA TGAAGAAGCA CTGGCAAACC GCCGCATCCT 
     4981 TCTCAGCTCT ACTGAAAGTC GAGAAGGCCT TGCACAGCAA GTTCAACAGA GTTTGGAAAA 
     5041 GATTTCTAAA CTGGAGCAGG AAAAAGAACA TTGGATGTTG GAAGCACAAT TAGCCAAAAT 
     5101 CAAGCTAGAG AAAGAAAACC AGCGAATTGC AGATAAGCTG AAGAATACAG GTAGTGCCCA 
     5161 GCTGGTTGGG CTGGCCCAGG AAAATGCTGC TGTGTCAAAT ACTGCTGGCC AGGATGAAGC 
     5221 CACAGCTAAG GCTGTGTTGG AGCCCATTCA GAGCACCAGT CTAATTGGGA CTTTAACCAG 
     5281 GACATCTGAC AGTGAGGTTC CAGATGTGGA ATCTCGTGAA GACTTAATTA AAAATCACTA 
     5341 CATGGCAAGG ATAGTGGAAC TTACGTCTCA GTTGCAGCTG GCTGACAGTA AGTCAGTGCA 
     5401 TTTTTATGCC GAGTGCCGAG CACTGTCTAA AAGACTGGCC TTGGCTGAAA AGTCTAAGGA 
     5461 AGCATTGACA GAAGAAATGA AACTTGCCAG TCAGAACATC AGCAGACTTC AGGATGAGCT 
     5521 GACAACTACC AAGAGGAGTT ACGAGGATCA GTTAAGTATG ATGAGTGACC ACCTGTGCAG 
     5581 CATGAATGAG ACATTATCTA AACAGAGAGA AGAGATTGAC ACACTAAAGA TGTCCAGTAA 
     5641 GGGGAATTCT AAAAAGAACA AGAGTCGAAC GCGTACGCGG CCGCTCGAGC AGAAACTCAT 
     5701 CTCAGAAGAG GATCTGGCAG CAAATGATAT CCTGGATTAC AAGGATGACG ACGATAAGGT 
     5761 TTGGGTAGGA AGCGGAGCTA CTAACTTCAG CCTGCTGAAG CAGGCTGGAG ACGTGGAGGA 
     5821 GAACCCTGGA CCTATGACCG AGTACAAGCC CACGGTGCGC CTCGCCACCC GCGACGACGT 
     5881 CCCCAGGGCC GTACGCACCC TCGCCGCCGC GTTCGCCGAC TACCCCGCCA CGCGCCACAC 
     5941 CGTCGATCCG GATCGCCACA TCGAGCGGGT CACCGAGCTG CAAGAACTCT TCCTCACGCG 
     6001 CGTCGGGCTC GACATCGGCA AGGTGTGGGT CGCGGACGAC GGCGCCGCGG TGGCGGTCTG 
     6061 GACCACGCCG GAGAGCGTCG AAGCGGGGGC GGTGTTCGCC GAGATCGGCC CGCGCATGGC 
     6121 CGAGTTGAGC GGTTCCCGGC TGGCCGCGCA GCAACAGATG GAAGGCCTCC TGGCGCCGCA 
     6181 CCGGCCCAAG GAGCCCGCGT GGTTCCTGGC CACCGTCGGC GTCTCGCCCG ACCACCAGGG 
     6241 CAAGGGTCTG GGCAGCGCCG TCGTGCTCCC CGGAGTGGAG GCGGCCGAGC GCGCCGGGGT 
     6301 GCCCGCCTTC CTGGAGACCT CCGCGCCCCG CAACCTCCCC TTCTACGAGC GGCTCGGCTT 
     6361 CACCGTCACC GCCGACGTCG AGGTGCCCGA AGGACCGCGC ACCTGGTGCA TGACCCGCAA 
     6421 GCCCGGTGCC TGATGTACAC CCAAACGGCC GGCCGCGGTC TGTACAAGTA GGATTCGTCG 
     6481 AGGGACCTAA TAACTTCGTA TAGCATACAT TATACGAAGT TATACATGTT TAAGGGTTCC 
     6541 GGTTCCACTA GGTACAATTC GATATCAAGC TTATCGATAA TCAACCTCTG GATTACAAAA 
     6601 TTTGTGAAAG ATTGACTGGT ATTCTTAACT ATGTTGCTCC TTTTACGCTA TGTGGATACG 
     6661 CTGCTTTAAT GCCTTTGTAT CATGCTATTG CTTCCCGTAT GGCTTTCATT TTCTCCTCCT 
     6721 TGTATAAATC CTGGTTGCTG TCTCTTTATG AGGAGTTGTG GCCCGTTGTC AGGCAACGTG 
     6781 GCGTGGTGTG CACTGTGTTT GCTGACGCAA CCCCCACTGG TTGGGGCATT GCCACCACCT 
     6841 GTCAGCTCCT TTCCGGGACT TTCGCTTTCC CCCTCCCTAT TGCCACGGCG GAACTCATCG 
     6901 CCGCCTGCCT TGCCCGCTGC TGGACAGGGG CTCGGCTGTT GGGCACTGAC AATTCCGTGG 
     6961 TGTTGTCGGG GAAATCATCG TCCTTTCCTT GGCTGCTCGC CTGTGTTGCC ACCTGGATTC 
     7021 TGCGCGGGAC GTCCTTCTGC TACGTCCCTT CGGCCCTCAA TCCAGCGGAC CTTCCTTCCC 
     7081 GCGGCCTGCT GCCGGCTCTG CGGCCTCTTC CGCGTCTTCG CCTTCGCCCT CAGACGAGTC 
     7141 GGATCTCCCT TTGGGCCGCC TCCCCGCATC GATACCGTCG ACCTCGATCG AGACCTAGAA 
     7201 AAACATGGAG CAATCACAAG TAGCAATACA GCAGCTACCA ATGCTGATTG TGCCTGGCTA 
     7261 GAAGCACAAG AGGAGGAGGA GGTGGGTTTT CCAGTCACAC CTCAGGTACC TTTAAGACCA 
     7321 ATGACTTACA AGGCAGCTGT AGATCTTAGC CACTTTTTAA AAGAAAAGGG GGGACTGGAA 
     7381 GGGCTAATTC ACTCCCAACG AAGACAAGAT ATCCTTGATC TGTGGATCTA CCACACACAA 
     7441 GGCTACTTCC CTGATTGGCA GAACTACACA CCAGGGCCAG GGATCAGATA TCCACTGACC 
     7501 TTTGGATGGT GCTACAAGCT AGTACCAGTT GAGCAAGAGA AGGTAGAAGA AGCCAATGAA 
     7561 GGAGAGAACA CCCGCTTGTT ACACCCTGTG AGCCTGCATG GGATGGATGA CCCGGAGAGA 
     7621 GAAGTATTAG AGTGGAGGTT TGACAGCCGC CTAGCATTTC ATCACATGGC CCGAGAGCTG 
     7681 CATCCGGACT GTACTGGGTC TCTCTGGTTA GACCAGATCT GAGCCTGGGA GCTCTCTGGC 
     7741 TAACTAGGGA ACCCACTGCT TAAGCCTCAA TAAAGCTTGC CTTGAGTGCT TCAAGTAGTG 
     7801 TGTGCCCGTC TGTTGTGTGA CTCTGGTAAC TAGAGATCCC TCAGACCCTT TTAGTCAGTG 
     7861 TGGAAAATCT CTAGCAGCAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG 
     7921 GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA 
     7981 CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT 
     8041 GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC 
     8101 TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG 
     8161 GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC 
     8221 TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA 
     8281 CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG 
     8341 TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT 
     8401 CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC 
     8461 ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA 
     8521 TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA 
     8581 CGTTAAGGGA TTTTGGTCAT GATTACGCCC CGCCCTGCCA CTCATCGCAG TACTGTTGTA 
     8641 ATTCATTAAG CATTCTGCCG ACATGGAAGC CATCACAAAC GGCATGATGA ACCTGAATCG 
     8701 CCAGCGGCAT CAGCACCTTG TCGCCTTGCG TATAATATTT GCCCATGGTG AAAACGGGGG 
     8761 CGAAGAAGTT GTCCATATTG GCCACGTTTA AATCAAAACT GGTGAAACTC ACCCAGGGAT 
     8821 TGGCTGAGAC GAAAAACATA TTCTCAATAA ACCCTTTAGG GAAATAGGCC AGGTTTTCAC 
     8881 CGTAACACGC CACATCTTGC GAATATATGT GTAGAAACTG CCGGAAATCG TCGTGGTATT 
     8941 CACTCCAGAG CGATGAAAAC GTTTCAGTTT GCTCATGGAA AACGGTGTAA CAAGGGTGAA 
     9001 CACTATCCCA TATCACCAGC TCACCGTCTT TCATTGCCAT ACGGAACTCC GGATGAGCAT 
     9061 TCATCAGGCG GGCAAGAATG TGAATAAAGG CCGGATAAAA CTTGTGCTTA TTTTTCTTTA 
     9121 CGGTCTTTAA AAAGGCCGTA ATATCCAGCT GAACGGTCTG GTTATAGGTA CATTGAGCAA 
     9181 CTGACTGAAA TGCCTCAAAA TGTTCTTTAC GATGCCATTG GGATATATCA ACGGTGGTAT 
     9241 ATCCAGTGAT TTTTTTCTCC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG 
     9301 GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG 
     9361 TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGAC
//