LOCUS       RC227703                6951 bp    DNA     circular  SYN 
DEFINITION  RASGRP3 (Myc-DDK-tagged)-Human RAS guanyl releasing protein 3 (calcium and DAG-regulated) (RASGRP3), transcript variant 1
ACCESSION   NM_001139488
VERSION     NM_001139488.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3108..3199 
                     /label="Myc-DDK"  
     misc_feature    3229..3814 
                     /label="PolyA signal"  
     misc_feature    3286..3305 
                     /label="Restriction Enzyme"  
     misc_feature    4090..4762 
                     /label="ColE1"  
     misc_feature    5192..5986 
                     /label="Kan/Neo"  
     misc_feature    6021..6323 
                     /label="SV40 ori"  
     misc_feature    6409..6886 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3090..3096 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3090 
                     /label="RASGRP3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGATCAAGT GGCCTTGGGA AAGCAGCAAC ATTAGATGAA CTGCTGTGCA 
     1081 CTTGCATTGA GATGTTTGAT GACAATGGAG AGCTGGATAA TAGTTATTTG CCAAGAATAG 
     1141 TTCTACTGAT GCACCGATGG TATTTATCTT CCACTGAATT GGCAGAAAAA CTTCTCTGCA 
     1201 TGTATCGAAA TGCCACTGGA GAAAGCTGCA ATGAATTTCG ATTAAAGATC TGCTACTTCA 
     1261 TGAGGTACTG GATTCTGAAG TTTCCTGCAG AGTTTAATTT GGATCTTGGT TTGATTCGTA 
     1321 TGACTGAGGA ATTTCGGGAA GTAGCTAGTC AACTAGGATA TGAAAAACAC GTCAGCCTCA 
     1381 TCGACATATC CAGCATTCCT TCCTATGACT GGATGAGAAG AGTCACACAG AGGAAAAAAG 
     1441 TATCCAAGAA GGGAAAAGCC TGTCTGCTGT TTGACCATCT GGAGCCCATT GAATTGGCTG 
     1501 AGCACCTCAC TTTTCTGGAG CATAAATCTT TTAGAAGGAT CTCATTCACT GATTACCAAA 
     1561 GCTATGTCAT CCATGGCTGC CTGGAGAATA ATCCAACCTT GGAAAGATCG ATTGCTTTAT 
     1621 TTAATGGAAT CTCTAAGTGG GTCCAGTTGA TGGTTCTTAG CAAACCAACC CCCCAGCAAA 
     1681 GGGCAGAAGT CATCACAAAG TTTATCAATG TTGCAAAGAA GCTCCTTCAG CTCAAAAATT 
     1741 TTAACACCCT GATGGCAGTG GTGGGAGGCC TCAGTCATAG TTCCATTTCA CGCCTCAAAG 
     1801 AGACCCATTC TCATCTTTCT TCAGAAGTTA CAAAGAACTG GAATGAAATG ACAGAGTTGG 
     1861 TCTCCTCCAA CGGCAATTAC TGCAATTACC GCAAGGCCTT TGCCGACTGC GATGGCTTCA 
     1921 AAATCCCCAT CCTTGGAGTA CACTTGAAAG ACTTGATAGC TGTCCATGTC ATTTTCCCAG 
     1981 ACTGGACAGA GGAGAACAAA GTGAACATTG TGAAAATGCA CCAGCTCTCC GTTACCCTGA 
     2041 GTGAACTAGT CTCCCTGCAG AATGCCTCTC ACCACTTAGA ACCCAACATG GATTTGATCA 
     2101 ACCTGCTCAC GCTTTCCCTG GACCTCTATC ACACTGAAGA TGATATTTAC AAACTGTCAC 
     2161 TGGTGCTGGA GCCTAGAAAT TCTAAATCGC AGCCTACCTC CCCTACGACG CCCAACAAGC 
     2221 CTGTGGTACC CCTGGAGTGG GCATTAGGGG TGATGCCAAA GCCAGACCCC ACGGTCATCA 
     2281 ACAAGCACAT AAGGAAATTA GTGGAGTCTG TATTTAGAAA CTATGATCAC GACCATGATG 
     2341 GGTACATTTC CCAAGAGGAC TTTGAAAGTA TAGCTGCCAA TTTTCCCTTC TTGGATTCCT 
     2401 TCTGTGTTCT GGACAAAGAT CAGGATGGCC TAATTAGTAA AGATGAAATG ATGGCTTACT 
     2461 TCCTGAGAGC TAAATCCCAA CTACACTGTA AAATGGGACC AGGATTTATC CATAATTTTC 
     2521 AGGAGATGAC CTATCTCAAG CCAACCTTCT GCGAACACTG TGCGGGATTT CTCTGGGGCA 
     2581 TAATCAAGCA AGGATACAAA TGCAAAGACT GTGGAGCCAA TTGTCACAAA CAGTGCAAAG 
     2641 ACCTCCTGGT TCTGGCCTGC AGGAGATTTG CCCGGGCGCC CTCCTTGAGC AGTGGTCATG 
     2701 GGTCACTGCC TGGAAGCCCC TCGCTGCCCC CAGCGCAGGA TGAGGTGTTT GAGTTCCCTG 
     2761 GAGTCACTGC TGGACACAGG GATTTAGACA GCAGAGCCAT CACACTGGTT ACAGGCTCTT 
     2821 CTCGCAAGAT CTCTGTGAGG CTACAGAGGG CCACCACCAG CCAGGCCACC CAGACTGAAC 
     2881 CTGTCTGGTC AGAGGCTGGC TGGGGGGACT CGGGGTCCCA CACCTTCCCT AAAATGAAAT 
     2941 CCAAGTTCCA TGACAAAGCA GCAAAGGACA AAGGCTTTGC CAAATGGGAA AATGAGAAGC 
     3001 CCAGGGTGCA TGCTGGTGTG GATGTTGTAG ACCGGGGCAC GGAGTTTGAA CTTGACCAGG 
     3061 ATGAAGGAGA AGAGACCAGA CAGGATGGTG AGGATGGCAC GCGTACGCGG CCGCTCGAGC 
     3121 AGAAACTCAT CTCAGAAGAG GATCTGGCAG CAAATGATAT CCTGGATTAC AAGGATGACG 
     3181 ACGATAAGGT TTAAACGGCC GGCCGCGGTC ATAGCTGTTT CCTGAACAGA TCCCGGGTGG 
     3241 CATCCCTGTG ACCCCTCCCC AGTGCCTCTC CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC 
     3301 ACCAGCCTTG TCCTAATAAA ATTAAGTTGC ATCATTTTGT CTGACTAGGT GTCCTTCTAT 
     3361 AATATTATGG GGTGGAGGGG GGTGGTATGG AGCAAGGGGC AAGTTGGGAA GACAACCTGT 
     3421 AGGGCCTGCG GGGTCTATTG GGAACCAAGC TGGAGTGCAG TGGCACAATC TTGGCTCACT 
     3481 GCAATCTCCG CCTCCTGGGT TCAAGCGATT CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA 
     3541 TTCCAGGCAT GCATGACCAG GCTCAGCTAA TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC 
     3601 ACCATATTGG CCAGGCTGGT CTCCAACTCC TAATCTCAGG TGATCTACCC ACCTTGGCCT 
     3661 CCCAAATTGC TGGGATTACA GGCGTGAACC ACTGCTCCCT TCCCTGTCCT TCTGATTTTA 
     3721 AAATAACTAT ACCAGCAGGA GGACGTCCAG ACACAGCATA GGCTACCTGG CCATGCCCAA 
     3781 CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC ACTGTCCTCT CATGCGTTGG GTCCACTCAG 
     3841 TAGATGCCTG TTGAATTGGG TACGCGGCCA GCGGCGAGCG GTATCAGCTC ACTCAAAGGC 
     3901 GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG 
     3961 CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG 
     4021 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG 
     4081 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC 
     4141 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA 
     4201 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT 
     4261 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC 
     4321 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG 
     4381 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC 
     4441 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT 
     4501 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA 
     4561 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG 
     4621 GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA 
     4681 AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT 
     4741 ATATGAGTAA CCTGAGGCTA TGGCAGGGCC TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG 
     4801 GCCTTCACCC GAACTTGGGG GGTGGGGTGG GGAAAAGGAA GAAACGCGGG CGTATTGGCC 
     4861 CCAATGGGGT CTCGGTGGGG TATCGACAGA GTGCCAGCCC TGGGACCGAA CCCCGCGTTT 
     4921 ATGAACAAAC GACCCAACAC CGTGCGTTTT ATTCTGTCTT TTTATTGCCG TCATAGCGCG 
     4981 GGTTCCTTCC GGTATTGTCT CCTTCCGTGT TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA 
     5041 GAACTCCAGC ATGAGATCCC CGCGCTGGAG GATCATCCAG CCGGCGTCCC GGAAAACGAT 
     5101 TCCGAAGCCC AACCTTTCAT AGAAGGCGGC GGTGGAATCG AAATCTCGTG ATGGCAGGTT 
     5161 GGGCGTCGCT TGGTCGGTCA TTTCGAACCC CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA 
     5221 AGGCGATAGA AGGCGATGCG CTGCGAATCG GGAGCGGCGA TACCGTAAAG CACGAGGAAG 
     5281 CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA GCAATATCAC GGGTAGCCAA CGCTATGTCC 
     5341 TGATAGCGAT CCGCCACACC CAGCCGGCCA CAGTCGATGA ATCCAGAAAA GCGGCCATTT 
     5401 TCCACCATGA TATTCGGCAA GCAGGCATCG CCATGGGTCA CGACGAGATC CTCGCCGTCG 
     5461 GGCATGCTCG CCTTGAGCCT GGCGAACAGT TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG 
     5521 TCCAGATCAT CCTGATCGAC AAGACCGGCT TCCATCCGAG TACGTGCTCG CTCGATGCGA 
     5581 TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA GCCGGATCAA GCGTATGCAG CCGCCGCATT 
     5641 GCATCAGCCA TGATGGATAC TTTCTCGGCA GGAGCAAGGT GAGATGACAG GAGATCCTGC 
     5701 CCCGGCACTT CGCCCAATAG CAGCCAGTCC CTTCCCGCTT CAGTGACAAC GTCGAGCACA 
     5761 GCTGCGCAAG GAACGCCCGT CGTGGCCAGC CACGATAGCC GCGCTGCCTC GTCTTGCAGT 
     5821 TCATTCAGGG CACCGGACAG GTCGGTCTTG ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC 
     5881 AGCCGGAACA CGGCGGCATC AGAGCAGCCG ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT 
     5941 AGCCTCTCCA CCCAAGCGGC CGGAGAACCT GCGTGCAATC CATCTTGTTC AATCATGCGA 
     6001 AACGATCCTC ATCCTGTCTC TTGATCGATC TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC 
     6061 TCCTCACTAC TTCTGGAATA GCTCAGAGGC CGAGGCGGCC TCGGCCTCTG CATAAATAAA 
     6121 AAAAATTAGT CAGCCATGGG GCGGAGAATG GGCGGAACTG GGCGGAGTTA GGGGCGGGAT 
     6181 GGGCGGAGTT AGGGGCGGGA CTATGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     6241 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCTGGTT GCTGACTAAT TGAGATGCAT 
     6301 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCCTA ACTGACACAC 
     6361 ATTCCACAGC TGGTTCTTTC CGCCTCAGGA CTCTTCCTTT TTCAATATTA TTGAAGCATT 
     6421 TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA 
     6481 ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGCGCCCTG TAGCGGCGCA 
     6541 TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA 
     6601 GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT 
     6661 CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC 
     6721 CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT 
     6781 TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA 
     6841 ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG 
     6901 GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT T
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