LOCUS       RC227674                9429 bp    DNA     circular  SYN 
DEFINITION  RUSC2 (Myc-DDK-tagged)-Human RUN and SH3 domain containing 2 (RUSC2)
ACCESSION   NM_001135999
VERSION     NM_001135999.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5586..5677 
                     /label="Myc-DDK"  
     misc_feature    5707..6292 
                     /label="PolyA signal"  
     misc_feature    5764..5783 
                     /label="Restriction Enzyme"  
     misc_feature    6568..7240 
                     /label="ColE1"  
     misc_feature    7670..8464 
                     /label="Kan/Neo"  
     misc_feature    8499..8801 
                     /label="SV40 ori"  
     misc_feature    8887..9364 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5568..5574 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5568 
                     /label="RUSC2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGATAGTCCC CCAAAGCTGA CTGGAGAGAC CCTCATCGTT CATCACATCC 
     1081 CCCTGGTGCA CTGCCAAGTC CCAGACAGGC AGTGCTGTGG AGGGGCAGGT GGAGGTGGTG 
     1141 GGAGCACAAG ACCTAATCCC TTCTGCCCAC CTGAGCTGGG CATCACCCAG CCCGATCAAG 
     1201 ACCTAGGACA AGCTGACTCC CTGCTATTCA GCAGCCTGCA CTCTGCTCCA GGAGGAACTG 
     1261 CACGGTCTAT AGACAGCACC AAGAGTAGGA GTCGGGATGG AAGAGGCCCT GGAGCCCCCA 
     1321 AACGACACAA CCCCTTCTTG CTGCAGGAGG GTGTGGGTGA GCCAGGACTT GGTGACCTGT 
     1381 ATGATGACAG CATTGGTGAC AGTGCCACCC AGCAGTCCTT CCACCTGCAT GGCACTGGCC 
     1441 AGCCCAACTT TCATCTATCC TCTTTCCAGC TGCCACCATC TGGCCCCAGA GTGGGCAGGC 
     1501 CATGGGGGAC AACACGCAGT CGGGCTGGAG TGGTGGAAGG GCAGGAACAG GAGCCAGTGA 
     1561 TGACCTTGGA TACTCAGCAG TGCGGCACCA GCCACTGCTG CCGGCCAGAG CTGGAAGCAG 
     1621 AGACTATGGA GCTGGATGAG TGTGGGGGAC CTGGTGGGAG TGGCAGTGGG GGTGGAGCCA 
     1681 GCGATACCTC TGGCTTTTCC TTTGACCAGG AATGGAAGCT CAGTTCAGAT GAATCCCCAA 
     1741 GGAACCCTGG ATGCTCCGGC TCAGGGGACC AGCACTGCCG CTGCAGTAGC ACATCCAGTC 
     1801 AGTCCGAGGC AGCTGACCAG TCCATGGGCT ATGTGAGCGA CTCCTCCTGC AACAGTTCAG 
     1861 ATGGTGTGCT GGTCACCTTC AGCACCCTCT ACAACAAGAT GCATGGCACC CCCCGTGCCA 
     1921 ATCTCAACTC TGCCCCACAG TCCTGCAGCG ACTCTTCCTT CTGCAGCCAC TCAGACCCTG 
     1981 GCGCCTTCTA TCTGGATCTG CAGCCCTCCC CATTTGAGTC TAAGATGTCT TATGAGTCCC 
     2041 ATCACCCTGA AAGTGGAGGA AGGGAAGGGG GCTATGGTTG CCCTCATGCC TCTTCTCCTG 
     2101 AGCTTGATGC CAACTGCAAC TCCTACCGCC CACACTGTGA GCCGTGCCCA GCAGTGGCTG 
     2161 ACCTCACAGC CTGCTTCCAA AGCCAGGCCC GTCTTGTTGT GGCCACACAA AATTACTATA 
     2221 AACTTGTCAC CTGTGACCTA TCTTCCCAAT CATCCCCAAG CCCTGCTGGC TCTTCCATCA 
     2281 CTAGCTGCTC TGAGGAACAC ACCAAGATAA GTCCCCCACC AGGCCCTGGC CCAGACCCAG 
     2341 GCCCCAGCCA GCCCTCTGAG TATTACCTAT TCCAGAAGCC AGAAGTCCAG CCAGAGGAAC 
     2401 AAGAAGCAGT GAGTTCCTCC ACCCAAGCAG CAGCTGCTGT GGGCCCCACT GTGCTTGAGG 
     2461 GACAAGTATA CACGAATACT TCACCCCCCA ACCTCAGCAC TGGACGTCAG CGCTCCCGCA 
     2521 GCTATGATCG CAGCCTGCAG CGCAGCCCTC CTGTCCGCCT GGGCTCGCTG GAACGTATGT 
     2581 TGAGTTGCCC AGTGCGCTTG AGTGAGGGCC CTGCAGCCAT GGCCGGGCCT GGCTCCCCAC 
     2641 CCAGGAGGGT CACCTCCTTT GCCGAGCTGG CCAAGGGCCG GAAGAAAACT GGAGGCTCTG 
     2701 GCTCGCCCCC ACTTCGTGTG AGTGTTGGGG ACTCCTCCCA GGAGTTCTCA CCCATCCAAG 
     2761 AAGCCCAGCA AGATCGGGGG GCCCCACTGG ATGAGGGCAC TTGCTGTAGC CATAGCCTGC 
     2821 CACCCATGCC TTTGGGGCCA GGCATGGACC TACTTGGCCC AGACCCAAGT CCACCCTGGT 
     2881 CCACCCAGGT CTGTCAGGGA CCCCACTCCA GTGAGATGCC TCCTGCTGGC CTCAGAGCTA 
     2941 CTGGGCAAGG CCCCCTGGCT CAGCTGATGG ATCCAGGGCC TGCTCTCCCA GGGAGCCCAG 
     3001 CCAACAGCCA TACCCAGAGG GATGCAAGAG CTAGAGCTGA CGGGGGTGGC ACCGAGAGCC 
     3061 GACCAGTCCT TCGCTACAGC AAGGAACAGA GGCCAACCAC ACTGCCCATC CAGCCCTTCG 
     3121 TGTTCCAGCA CCACTTCCCC AAGCAGCTGG CCAAGGCCCG GGCCCTCCAC AGCCTTTCCC 
     3181 AGCTCTACAG CCTCTCAGGC TGCAGCCGTA CACAGCAGCC TGCCCCACTG GCTGCCCCTG 
     3241 CTGCTCAAGT CTCAGTCCCA GCTCCCTCAG GGGAACCGCA GGCATCCACT CCCCGAGCCA 
     3301 CTGGCAGAGG TGCCAGGAAA GCTGGGTCTG AGCCAGAGAC CTCTCGGCCA TCGCCCCTGG 
     3361 GCAGCTACTC CCCCATCCGG AGTGTTGGCC CCTTTGGGCC CAGCACTGAC TCTTCTGCCT 
     3421 CCACTTCGTG CTCCCCTCCC CCAGAGCAGC CCACAGCCAC AGAAAGCCTG CCCCCATGGA 
     3481 GCCACTCCTG TCCTTCTGCT GTCCGGCCTG CCACCTCCCA GCAGCCGCAG AAGGAGGATC 
     3541 AGAAGATACT GACCTTGACT GAGTACCGGC TCCATGGAAC AGGAAGCTTG CCGCCTCTGG 
     3601 GCTCCTGGCG ATCTGGCCTC AGCCGAGCAG AGAGCCTGGC CCGGGGAGGT GGTGAGGGCA 
     3661 GCATGGCCAC CAGGCCCAGT AATGCCAACC ACCTATCCCC TCAAGCCCTC AAGTGGCGGG 
     3721 AATACAGGAG GAAGAACCCA CTAGGGCCAC CTGGTTTGTC AGGGAGCCTA GACCGAAGAT 
     3781 CACAAGAAGC TCGGCTGGCC CGAAGAAACC CTATCTTTGA GTTCCCTGGC TCCCTCAGTG 
     3841 CTGCCAGCCA TCTGAACTGC CGGCTGAATG GCCAAGCAGT GAAGCCGTTA CCACTGACCT 
     3901 GCCCTGACTT CCAGGACCCC TTTTCCTTGA CGGAGAAGCC TCCAGCTGAG TTTTGTCTGT 
     3961 CCCCAGATGG CAGCTCAGAG GCCATTTCCA TTGACCTGCT TCAGAAAAAA GGGCTGGTAA 
     4021 AAGCTGTTAA CATCGCTGTG GACCTCATTG TGGCTCATTT TGGCACAAGC CGGGATCCCG 
     4081 GGGTGAAGGC AAAGCTGGGA AACAGTTCTG TGAGCCCCAA TGTGGGCCAC CTGGTTCTGA 
     4141 AGTACTTGTG CCCTGCCGTC CGCGCCGTGC TGGAGGATGG GCTCAAGGCC TTTGTACTGG 
     4201 ACGTCATCAT CGGGCAGCGT AAGAACATGC CATGGAGTGT GGTTGAGGCT TCCACACAGC 
     4261 TAGGCCCATC CACCAAGGTC CTGCATGGCC TCTACAACAA AGTCAGCCAA TTCCCAGAGC 
     4321 TCACCAGTCA TACCATGCGC TTCAACGCCT TCATCCTCGG CCTGCTCAAC ATCCGGTCCC 
     4381 TGGAGTTCTG GTTTAATCAC CTCTATAACC ACGAAGACAT CATCCAGACC CACTACCAGC 
     4441 CCTGGGGCTT CCTGAGTGCA GCTCATACCG TGTGTCCCGG CCTCTTTGAA GAGCTGCTGC 
     4501 TGCTGCTACA GCCCCTGGCC CTGCTGCCCT TCAGCCTCGA CTTGCTGTTC CAGCACCGGC 
     4561 TGCTGCAAAG TGGGCAGCAG CAGCGGCAGC ACAAGGAACT GCTGCGGGTG TCCCAGGACC 
     4621 TGCTGCTGTC TGCCCACTCC ACGCTGCAGC TGGCCCGGGC CCGGGGCCAG GAGGGCCCTG 
     4681 GAGACGTGGA CAGGGCAGCC CAAGGGGAGC GGGTGAAGGG TGTGGGTGCC TCAGAAGGTG 
     4741 GAGAAGAGGA AGAGGAAGAA GAGGAGACAG AAGAGGTGGC AGAGGCAGCC GGGGGCTCAG 
     4801 GGCGTGCCAG GTGGGCCCGA GGTGGGCAGG CCGGCTGGTG GTACCAGCTC ATGCAGAGCT 
     4861 CCCAGGTCTA CATCGATGGC TCCATTGAGG GTTCCAGGTT CCCTCGTGGT AGCAGCAACA 
     4921 GCAGCAGCGA GAAAAAGAAA GGGGCAGGAG GTGGGGGACC TCCCCAGGCT CCACCACCCC 
     4981 GAGAGGGAGT AGTGGAGGGG GCTGAGGCCT GCCCTGCCTC TGAGGAGGCC CTGGGCCGGG 
     5041 AAAGGGGCTG GCCCTTCTGG ATGGGGAGCC CCCCTGACTC TGTGCTGGCC GAGCTGAGGC 
     5101 GCAGTCGGGA GAGGGAAGGG CCCGCTGCCT CGCCAGCAGA AAATGAGGAA GGGGCCTCAG 
     5161 AGCCTTCACC TGGAGGCATC AAGTGGGGAC ACCTCTTTGG CTCCCGAAAA GCCCAGCGGG 
     5221 AGGCCCGGCC CACAAATAGG CTCCCCTCGG ACTGGCTGAG CCTGGACAAG TCCATGTTCC 
     5281 AACTAGTGGC GCAGACAGTG GGTTCCCGCC GGGAGCCAGA GCCCAAGGAG AGCCTGCAGG 
     5341 AGCCACACTC CCCAGCCCTG CCCTCCAGTC CTCCGTGTGA GGTGCAGGCA CTGTGCCACC 
     5401 ACCTGGCCAC CGGCCCTGGA CAGCTGAGCT TCCACAAAGG AGACATCCTA CGAGTGCTGG 
     5461 GGCGAGCTGG AGGAGACTGG CTGCGCTGCA GCCGTGGCCC CGACTCTGGC CTGGTGCCCC 
     5521 TGGCCTACGT GACATTGACC CCAACTCCAA GTCCAACCCC TGGAAGCAGC CAAAACACGC 
     5581 GTACGCGGCC GCTCGAGCAG AAACTCATCT CAGAAGAGGA TCTGGCAGCA AATGATATCC 
     5641 TGGATTACAA GGATGACGAC GATAAGGTTT AAACGGCCGG CCGCGGTCAT AGCTGTTTCC 
     5701 TGAACAGATC CCGGGTGGCA TCCCTGTGAC CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA 
     5761 GTTGCCACTC CAGTGCCCAC CAGCCTTGTC CTAATAAAAT TAAGTTGCAT CATTTTGTCT 
     5821 GACTAGGTGT CCTTCTATAA TATTATGGGG TGGAGGGGGG TGGTATGGAG CAAGGGGCAA 
     5881 GTTGGGAAGA CAACCTGTAG GGCCTGCGGG GTCTATTGGG AACCAAGCTG GAGTGCAGTG 
     5941 GCACAATCTT GGCTCACTGC AATCTCCGCC TCCTGGGTTC AAGCGATTCT CCTGCCTCAG 
     6001 CCTCCCGAGT TGTTGGGATT CCAGGCATGC ATGACCAGGC TCAGCTAATT TTTGTTTTTT 
     6061 TGGTAGAGAC GGGGTTTCAC CATATTGGCC AGGCTGGTCT CCAACTCCTA ATCTCAGGTG 
     6121 ATCTACCCAC CTTGGCCTCC CAAATTGCTG GGATTACAGG CGTGAACCAC TGCTCCCTTC 
     6181 CCTGTCCTTC TGATTTTAAA ATAACTATAC CAGCAGGAGG ACGTCCAGAC ACAGCATAGG 
     6241 CTACCTGGCC ATGCCCAACC GGTGGGACAT TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA 
     6301 TGCGTTGGGT CCACTCAGTA GATGCCTGTT GAATTGGGTA CGCGGCCAGC GGCGAGCGGT 
     6361 ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA 
     6421 GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC 
     6481 GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG 
     6541 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT 
     6601 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG 
     6661 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG 
     6721 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG 
     6781 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC 
     6841 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG 
     6901 GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT 
     6961 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG 
     7021 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC 
     7081 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT 
     7141 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT 
     7201 TAAATCAATC TAAAGTATAT ATGAGTAACC TGAGGCTATG GCAGGGCCTG CCGCCCCGAC 
     7261 GTTGGCTGCG AGCCCTGGGC CTTCACCCGA ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA 
     7321 AACGCGGGCG TATTGGCCCC AATGGGGTCT CGGTGGGGTA TCGACAGAGT GCCAGCCCTG 
     7381 GGACCGAACC CCGCGTTTAT GAACAAACGA CCCAACACCG TGCGTTTTAT TCTGTCTTTT 
     7441 TATTGCCGTC ATAGCGCGGG TTCCTTCCGG TATTGTCTCC TTCCGTGTTT CAGTTAGCCT 
     7501 CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT GAGATCCCCG CGCTGGAGGA TCATCCAGCC 
     7561 GGCGTCCCGG AAAACGATTC CGAAGCCCAA CCTTTCATAG AAGGCGGCGG TGGAATCGAA 
     7621 ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG GTCGGTCATT TCGAACCCCA GAGTCCCGCT 
     7681 CAGAAGAACT CGTCAAGAAG GCGATAGAAG GCGATGCGCT GCGAATCGGG AGCGGCGATA 
     7741 CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT TCGCCGCCAA GCTCTTCAGC AATATCACGG 
     7801 GTAGCCAACG CTATGTCCTG ATAGCGATCC GCCACACCCA GCCGGCCACA GTCGATGAAT 
     7861 CCAGAAAAGC GGCCATTTTC CACCATGATA TTCGGCAAGC AGGCATCGCC ATGGGTCACG 
     7921 ACGAGATCCT CGCCGTCGGG CATGCTCGCC TTGAGCCTGG CGAACAGTTC GGCTGGCGCG 
     7981 AGCCCCTGAT GCTCTTCGTC CAGATCATCC TGATCGACAA GACCGGCTTC CATCCGAGTA 
     8041 CGTGCTCGCT CGATGCGATG TTTCGCTTGG TGGTCGAATG GGCAGGTAGC CGGATCAAGC 
     8101 GTATGCAGCC GCCGCATTGC ATCAGCCATG ATGGATACTT TCTCGGCAGG AGCAAGGTGA 
     8161 GATGACAGGA GATCCTGCCC CGGCACTTCG CCCAATAGCA GCCAGTCCCT TCCCGCTTCA 
     8221 GTGACAACGT CGAGCACAGC TGCGCAAGGA ACGCCCGTCG TGGCCAGCCA CGATAGCCGC 
     8281 GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA CCGGACAGGT CGGTCTTGAC AAAAAGAACC 
     8341 GGGCGCCCCT GCGCTGACAG CCGGAACACG GCGGCATCAG AGCAGCCGAT TGTCTGTTGT 
     8401 GCCCAGTCAT AGCCGAATAG CCTCTCCACC CAAGCGGCCG GAGAACCTGC GTGCAATCCA 
     8461 TCTTGTTCAA TCATGCGAAA CGATCCTCAT CCTGTCTCTT GATCGATCTT TGCAAAAGCC 
     8521 TAGGCCTCCA AAAAAGCCTC CTCACTACTT CTGGAATAGC TCAGAGGCCG AGGCGGCCTC 
     8581 GGCCTCTGCA TAAATAAAAA AAATTAGTCA GCCATGGGGC GGAGAATGGG CGGAACTGGG 
     8641 CGGAGTTAGG GGCGGGATGG GCGGAGTTAG GGGCGGGACT ATGGTTGCTG ACTAATTGAG 
     8701 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCTGGTTGC 
     8761 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     8821 ACACCCTAAC TGACACACAT TCCACAGCTG GTTCTTTCCG CCTCAGGACT CTTCCTTTTT 
     8881 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT 
     8941 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC 
     9001 GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT 
     9061 ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG 
     9121 TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT 
     9181 GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA 
     9241 TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA 
     9301 CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA 
     9361 GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC 
     9421 GCGAATTTT
//