LOCUS       RC227443                7479 bp    DNA     circular  SYN 
DEFINITION  NELL2 (Myc-DDK-tagged)-Human NEL-like 2 (chicken) (NELL2), transcript variant 1
ACCESSION   NM_001145107
VERSION     NM_001145107.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3636..3727 
                     /label="Myc-DDK"  
     misc_feature    3757..4342 
                     /label="PolyA signal"  
     misc_feature    3814..3833 
                     /label="Restriction Enzyme"  
     misc_feature    4618..5290 
                     /label="ColE1"  
     misc_feature    5720..6514 
                     /label="Kan/Neo"  
     misc_feature    6549..6851 
                     /label="SV40 ori"  
     misc_feature    6937..7414 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3618..3624 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3618 
                     /label="NELL2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGCTTCCCT CCTTTGCTTA AAGGGCAGGC GAGCGCTACT CGCTCCAGCC 
     1081 TTGCCTCCTG CAGCTGGGTG GTCTTTTTTC TCTCCTGTCT TTCAAGACAC GCGCCCGAAA 
     1141 TCGAGGGAGG GAGACGATGG ACTGAGCTGA TCCGCACCAT GGAGTCTCGG GTCTTACTGA 
     1201 GAACATTCTG TTTGATCTTC GGTCTCGGAG CAGTTTGGGG GCTTGGTGTG GACCCTTCCC 
     1261 TACAGATTGA CGTCTTAACA GAGTTAGAAC TTGGGGAGTC CACGACCGGA GTGCGTCAGG 
     1321 TCCCGGGGCT GCATAATGGG ACGAAAGCCT TTCTCTTTCA AGATACTCCC AGAAGCATAA 
     1381 AAGCATCCAC TGCTACAGCT GAACAGTTTT TTCAGAAGCT GAGAAATAAA CATGAATTTA 
     1441 CTATTTTGGT GACCCTAAAA CAGACCCACT TAAATTCAGG AGTTATTCTC TCAATTCACC 
     1501 ACTTGGATCA CAGGTACCTG GAACTGGAAA GTAGTGGCCA TCGGAATGAA GTCAGACTGC 
     1561 ATTACCGCTC AGGCAGTCAC CGCCCTCACA CAGAAGTGTT TCCTTACATT TTGGCTGATG 
     1621 ACAAGTGGCA CAAGCTCTCC TTAGCCATCA GTGCTTCCCA TTTGATTTTA CACATTGACT 
     1681 GCAATAAAAT TTATGAAAGG GTAGTAGAAA AGCCCTCCAC AGACTTGCCT CTAGGCACAA 
     1741 CATTTTGGCT AGGACAGAGA AATAATGCGC ATGGATATTT TAAGGGTATA ATGCAAGATG 
     1801 TCCAATTACT TGTCATGCCC CAGGGATTTA TTGCTCAGTG CCCAGATCTT AATCGCACCT 
     1861 GTCCAACTTG CAATGACTTC CATGGACTTG TGCAGAAAAT CATGGAGCTA CAGGATATTT 
     1921 TAGCCAAAAC ATCAGCCAAG CTGTCTCGAG CTGAACAGCG AATGAATAGA TTGGATCAGT 
     1981 GCTATTGTGA AAGGACTTGC ACCATGAAGG GAACCACCTA CCGAGAATTT GAGTCCTGGA 
     2041 TAGACGGCTG TAAGAACTGC ACATGCCTGA ATGGAACCAT CCAGTGTGAA ACTCTAATCT 
     2101 GCCCAAATCC TGACTGCCCA CTTAAGTCGG CTCTTGCGTA TGTGGATGGC AAATGCTGTA 
     2161 AGGAATGCAA ATCGATATGC CAATTTCAAG GACGAACCTA CTTTGAAGGA GAAAGAAATA 
     2221 CAGTCTATTC CTCTTCTGGA GTATGTGTTC TCTATGAGTG CAAGGACCAG ACCATGAAAC 
     2281 TTGTTGAGAG TTCAGGCTGT CCAGCTTTGG ATTGTCCAGA GTCTCATCAG ATAACCTTGT 
     2341 CTCACAGCTG TTGCAAAGTT TGTAAAGGTT ATGACTTTTG TTCTGAAAGG CATAACTGCA 
     2401 TGGAGAATTC CATCTGCAGA AATCTGAATG ACAGGGCTGT TTGTAGCTGT CGAGATGGTT 
     2461 TTAGGGCTCT TCGAGAGGAT AATGCCTACT GTGAAGACAT CGATGAGTGT GCTGAAGGGC 
     2521 GCCATTACTG TCGTGAAAAT ACAATGTGTG TCAACACCCC GGGTTCTTTT ATGTGCATCT 
     2581 GCAAAACTGG ATACATCAGA ATTGATGATT ATTCATGTAC AGAACATGAT GAGTGTATCA 
     2641 CAAATCAGCA CAACTGTGAT GAAAATGCTT TATGCTTCAA CACTGTTGGA GGACACAACT 
     2701 GTGTTTGCAA GCCGGGCTAT ACAGGGAATG GAACGACATG CAAAGCATTT TGCAAAGATG 
     2761 GCTGTAGGAA TGGAGGAGCC TGTATTGCCG CTAATGTGTG TGCCTGCCCA CAAGGCTTCA 
     2821 CTGGACCCAG CTGTGAAACG GACATTGATG AATGCTCTGA TGGTTTTGTT CAATGTGACA 
     2881 GTCGTGCTAA TTGCATTAAC CTGCCTGGAT GGTACCACTG TGAGTGCAGA GATGGCTACC 
     2941 ATGACAATGG GATGTTTTCA CCAAGTGGAG AATCGTGTGA AGATATTGAT GAGTGTGGGA 
     3001 CCGGGAGGCA CAGCTGTGCC AATGATACCA TTTGCTTCAA TTTGGATGGC GGATATGATT 
     3061 GTCGATGTCC TCATGGAAAG AATTGCACAG GGGACTGCAT CCATGATGGA AAAGTTAAGC 
     3121 ACAATGGTCA GATTTGGGTG TTGGAAAATG ACAGGTGCTC TGTGTGCTCA TGTCAGAATG 
     3181 GATTCGTTAT GTGTCGACGG ATGGTCTGTG ACTGTGAGAA TCCCACAGTT GATCTTTTTT 
     3241 GCTGCCCTGA ATGTGACCCA AGGCTTAGTA GTCAGTGCCT CCATCAAAAT GGGGAAACTT 
     3301 TGTATAACAG TGGTGACACC TGGGTCCAGA ATTGTCAACA GTGCCGCTGC TTGCAAGGGG 
     3361 AAGTTGATTG TTGGCCCCTG CCTTGCCCAG ATGTGGAGTG TGAATTCAGC ATTCTCCCAG 
     3421 AGAATGAGTG CTGCCCGCGC TGTGTCACAG ACCCTTGCCA GGCTGACACC ATCCGCAATG 
     3481 ACATCACCAA GACTTGCCTG GACGAAATGA ATGTGGTTCG CTTCACCGGG TCCTCTTGGA 
     3541 TCAAACATGG CACTGAGTGT ACTCTCTGCC AGTGCAAGAA TGGCCACATC TGTTGCTCAG 
     3601 TGGATCCACA GTGCCTTCAG GAACTGACGC GTACGCGGCC GCTCGAGCAG AAACTCATCT 
     3661 CAGAAGAGGA TCTGGCAGCA AATGATATCC TGGATTACAA GGATGACGAC GATAAGGTTT 
     3721 AAACGGCCGG CCGCGGTCAT AGCTGTTTCC TGAACAGATC CCGGGTGGCA TCCCTGTGAC 
     3781 CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA GTTGCCACTC CAGTGCCCAC CAGCCTTGTC 
     3841 CTAATAAAAT TAAGTTGCAT CATTTTGTCT GACTAGGTGT CCTTCTATAA TATTATGGGG 
     3901 TGGAGGGGGG TGGTATGGAG CAAGGGGCAA GTTGGGAAGA CAACCTGTAG GGCCTGCGGG 
     3961 GTCTATTGGG AACCAAGCTG GAGTGCAGTG GCACAATCTT GGCTCACTGC AATCTCCGCC 
     4021 TCCTGGGTTC AAGCGATTCT CCTGCCTCAG CCTCCCGAGT TGTTGGGATT CCAGGCATGC 
     4081 ATGACCAGGC TCAGCTAATT TTTGTTTTTT TGGTAGAGAC GGGGTTTCAC CATATTGGCC 
     4141 AGGCTGGTCT CCAACTCCTA ATCTCAGGTG ATCTACCCAC CTTGGCCTCC CAAATTGCTG 
     4201 GGATTACAGG CGTGAACCAC TGCTCCCTTC CCTGTCCTTC TGATTTTAAA ATAACTATAC 
     4261 CAGCAGGAGG ACGTCCAGAC ACAGCATAGG CTACCTGGCC ATGCCCAACC GGTGGGACAT 
     4321 TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA TGCGTTGGGT CCACTCAGTA GATGCCTGTT 
     4381 GAATTGGGTA CGCGGCCAGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT 
     4441 ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC 
     4501 CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA 
     4561 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA 
     4621 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC 
     4681 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG 
     4741 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC 
     4801 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG 
     4861 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT 
     4921 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT 
     4981 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG 
     5041 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC 
     5101 GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA 
     5161 GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC 
     5221 CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAACC 
     5281 TGAGGCTATG GCAGGGCCTG CCGCCCCGAC GTTGGCTGCG AGCCCTGGGC CTTCACCCGA 
     5341 ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA AACGCGGGCG TATTGGCCCC AATGGGGTCT 
     5401 CGGTGGGGTA TCGACAGAGT GCCAGCCCTG GGACCGAACC CCGCGTTTAT GAACAAACGA 
     5461 CCCAACACCG TGCGTTTTAT TCTGTCTTTT TATTGCCGTC ATAGCGCGGG TTCCTTCCGG 
     5521 TATTGTCTCC TTCCGTGTTT CAGTTAGCCT CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT 
     5581 GAGATCCCCG CGCTGGAGGA TCATCCAGCC GGCGTCCCGG AAAACGATTC CGAAGCCCAA 
     5641 CCTTTCATAG AAGGCGGCGG TGGAATCGAA ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG 
     5701 GTCGGTCATT TCGAACCCCA GAGTCCCGCT CAGAAGAACT CGTCAAGAAG GCGATAGAAG 
     5761 GCGATGCGCT GCGAATCGGG AGCGGCGATA CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT 
     5821 TCGCCGCCAA GCTCTTCAGC AATATCACGG GTAGCCAACG CTATGTCCTG ATAGCGATCC 
     5881 GCCACACCCA GCCGGCCACA GTCGATGAAT CCAGAAAAGC GGCCATTTTC CACCATGATA 
     5941 TTCGGCAAGC AGGCATCGCC ATGGGTCACG ACGAGATCCT CGCCGTCGGG CATGCTCGCC 
     6001 TTGAGCCTGG CGAACAGTTC GGCTGGCGCG AGCCCCTGAT GCTCTTCGTC CAGATCATCC 
     6061 TGATCGACAA GACCGGCTTC CATCCGAGTA CGTGCTCGCT CGATGCGATG TTTCGCTTGG 
     6121 TGGTCGAATG GGCAGGTAGC CGGATCAAGC GTATGCAGCC GCCGCATTGC ATCAGCCATG 
     6181 ATGGATACTT TCTCGGCAGG AGCAAGGTGA GATGACAGGA GATCCTGCCC CGGCACTTCG 
     6241 CCCAATAGCA GCCAGTCCCT TCCCGCTTCA GTGACAACGT CGAGCACAGC TGCGCAAGGA 
     6301 ACGCCCGTCG TGGCCAGCCA CGATAGCCGC GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA 
     6361 CCGGACAGGT CGGTCTTGAC AAAAAGAACC GGGCGCCCCT GCGCTGACAG CCGGAACACG 
     6421 GCGGCATCAG AGCAGCCGAT TGTCTGTTGT GCCCAGTCAT AGCCGAATAG CCTCTCCACC 
     6481 CAAGCGGCCG GAGAACCTGC GTGCAATCCA TCTTGTTCAA TCATGCGAAA CGATCCTCAT 
     6541 CCTGTCTCTT GATCGATCTT TGCAAAAGCC TAGGCCTCCA AAAAAGCCTC CTCACTACTT 
     6601 CTGGAATAGC TCAGAGGCCG AGGCGGCCTC GGCCTCTGCA TAAATAAAAA AAATTAGTCA 
     6661 GCCATGGGGC GGAGAATGGG CGGAACTGGG CGGAGTTAGG GGCGGGATGG GCGGAGTTAG 
     6721 GGGCGGGACT ATGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     6781 GGAGCCTGGG GACTTTCCAC ACCTGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     6841 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCCTAAC TGACACACAT TCCACAGCTG 
     6901 GTTCTTTCCG CCTCAGGACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT 
     6961 TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG 
     7021 CGCACATTTC CCCGAAAAGT GCCACCTGAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG 
     7081 GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT 
     7141 TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT 
     7201 CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT 
     7261 GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG 
     7321 ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC 
     7381 CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA 
     7441 AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTTT
//