LOCUS       RC227442                6693 bp    DNA     circular  SYN 
DEFINITION  BEST1 (Myc-DDK-tagged)-Human bestrophin 1 (BEST1), transcript variant 2
ACCESSION   NM_001139443
VERSION     NM_001139443.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    2850..2941 
                     /label="Myc-DDK"  
     misc_feature    2971..3556 
                     /label="PolyA signal"  
     misc_feature    3028..3047 
                     /label="Restriction Enzyme"  
     misc_feature    3832..4504 
                     /label="ColE1"  
     misc_feature    4934..5728 
                     /label="Kan/Neo"  
     misc_feature    5763..6065 
                     /label="SV40 ori"  
     misc_feature    6151..6628 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    2832..2838 
                     /label="Restriction Enzyme"  
     misc_feature    1020..2832 
                     /label="BEST1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTTTGAGAAA CTGACTCTGT ATTGCGACAG CTACATCCAG CTCATCCCCA 
     1081 TTTCCTTCGT GCTGGGCTTC TACGTGACGC TGGTCGTGAC CCGCTGGTGG AACCAGTACG 
     1141 AGAACCTGCC GTGGCCCGAC CGCCTCATGA GCCTGGTGTC GGGCTTCGTC GAAGGCAAGG 
     1201 ACGAGCAAGG CCGGCTGCTG CGGCGCACGC TCATCCGCTA CGCCAACCTG GGCAACGTGC 
     1261 TCATCCTGCG CAGCGTCAGC ACCGCAGTCT ACAAGCGCTT CCCCAGCGCC CAGCACCTGG 
     1321 TGCAAGCAGG CTTTATGACT CCGGCAGAAC ACAAGCAGTT GGAGAAACTG AGCCTACCAC 
     1381 ACAACATGTT CTGGGTGCCC TGGGTGTGGT TTGCCAACCT GTCAATGAAG GCGTGGCTTG 
     1441 GAGGTCGAAT CCGGGACCCT ATCCTGCTCC AGAGCCTGCT GAACGAGATG AACACCTTGC 
     1501 GTACTCAGTG TGGACACCTG TATGCCTACG ACTGGATTAG TATCCCACTG GTGTATACAC 
     1561 AGGTGGTGAC TGTGGCGGTG TACAGCTTCT TCCTGACTTG TCTAGTTGGG CGGCAGTTTC 
     1621 TGAACCCAGC CAAGGCCTAC CCTGGCCATG AGCTGGACCT CGTTGTGCCC GTCTTCACGT 
     1681 TCCTGCAGTT CTTCTTCTAT GTTGGCTGGC TGAAGGTGGC AGAGCAGCTC ATCAACCCCT 
     1741 TTGGAGAGGA TGATGATGAT TTTGAGACCA ACTGGATTGT CGACAGGAAT TTGCAGGTGT 
     1801 CCCTGTTGGC TGTGGATGAG ATGCACCAGG ACCTGCCTCG GATGGAGCCG GACATGTACT 
     1861 GGAATAAGCC CGAGCCACAG CCCCCCTACA CAGCTGCTTC CGCCCAGTTC CGTCGAGTCT 
     1921 CCTTTATGGG CTCCACCTTC AACATCAGCC TGAACAAAGA GGAGATGGAG TTCCAGCCCA 
     1981 ATCAGGAGGA CGAGGAGGAT GCTCACGCTG GCATCATTGG CCGCTTCCTA GGCCTGCAGT 
     2041 CCCATGATCA CCATCCTCCC AGGGCAAACT CAAGGACCAA ACTACTGTGG CCCAAGAGGG 
     2101 AATCCCTTCT CCACGAGGGC CTGCCCAAAA ACCACAAGGC AGCCAAACAG AACGTTAGGG 
     2161 GCCAGGAAGA CAACAAGGCC TGGAAGCTTA AGGCTGTGGA CGCCTTCAAG TCTGCCCCAC 
     2221 TGTATCAGAG GCCAGGCTAC TACAGTGCCC CACAGACGCC CCTCAGCCCC ACTCCCATGT 
     2281 TCTTCCCCCT AGAACCATCA GCGCCGTCAA AGCTTCACAG TGTCACAGGC ATAGACACCA 
     2341 AAGACAAAAG CTTAAAGACT GTGAGTTCTG GGGCCAAAAA AAGTTTTGAA TTGCTCTCAG 
     2401 AGAGCGATGG GGCCTTGATG GAGCACCCAG AAGTATCTCA AGTGAGGAGG AAAACTGTGG 
     2461 AGTTTAACCT GACGGATATG CCAGAGATCC CCGAAAATCA CCTCAAAGAA CCTTTGGAAC 
     2521 AATCACCAAC CAACATACAC ACTACACTCA AAGATCACAT GGATCCTTAT TGGGCCTTGG 
     2581 AAAACAGGTC TGTCCTCCAC CTGAACCAGG GGCACTGCAT TGCCCTGTGC CCCACCCCAG 
     2641 CTTCCCTTGC TCTGAGCCTA CCCTTCCTCC ACAATTTCCT AGGGTTCCAT CACTGCCAGA 
     2701 GCACACTGGA CCTACGCCCA GCACTGGCTT GGGGTATATA CTTGGCCACC TTCACAGGGA 
     2761 TCCTAGGGAA GTGTTCGGGA CCTTTTCTCA CTTCACCCTG GTATCACCCG GAAGACTTCT 
     2821 TGGGACCAGG TGAAGGAAGA ACGCGTACGC GGCCGCTCGA GCAGAAACTC ATCTCAGAAG 
     2881 AGGATCTGGC AGCAAATGAT ATCCTGGATT ACAAGGATGA CGACGATAAG GTTTAAACGG 
     2941 CCGGCCGCGG TCATAGCTGT TTCCTGAACA GATCCCGGGT GGCATCCCTG TGACCCCTCC 
     3001 CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA 
     3061 AAATTAAGTT GCATCATTTT GTCTGACTAG GTGTCCTTCT ATAATATTAT GGGGTGGAGG 
     3121 GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG AAGACAACCT GTAGGGCCTG CGGGGTCTAT 
     3181 TGGGAACCAA GCTGGAGTGC AGTGGCACAA TCTTGGCTCA CTGCAATCTC CGCCTCCTGG 
     3241 GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC GAGTTGTTGG GATTCCAGGC ATGCATGACC 
     3301 AGGCTCAGCT AATTTTTGTT TTTTTGGTAG AGACGGGGTT TCACCATATT GGCCAGGCTG 
     3361 GTCTCCAACT CCTAATCTCA GGTGATCTAC CCACCTTGGC CTCCCAAATT GCTGGGATTA 
     3421 CAGGCGTGAA CCACTGCTCC CTTCCCTGTC CTTCTGATTT TAAAATAACT ATACCAGCAG 
     3481 GAGGACGTCC AGACACAGCA TAGGCTACCT GGCCATGCCC AACCGGTGGG ACATTTGAGT 
     3541 TGCTTGCTTG GCACTGTCCT CTCATGCGTT GGGTCCACTC AGTAGATGCC TGTTGAATTG 
     3601 GGTACGCGGC CAGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC 
     3661 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA 
     3721 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 
     3781 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 
     3841 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA 
     3901 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA 
     3961 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA 
     4021 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 
     4081 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 
     4141 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG 
     4201 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 
     4261 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG 
     4321 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA 
     4381 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT 
     4441 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AACCTGAGGC 
     4501 TATGGCAGGG CCTGCCGCCC CGACGTTGGC TGCGAGCCCT GGGCCTTCAC CCGAACTTGG 
     4561 GGGGTGGGGT GGGGAAAAGG AAGAAACGCG GGCGTATTGG CCCCAATGGG GTCTCGGTGG 
     4621 GGTATCGACA GAGTGCCAGC CCTGGGACCG AACCCCGCGT TTATGAACAA ACGACCCAAC 
     4681 ACCGTGCGTT TTATTCTGTC TTTTTATTGC CGTCATAGCG CGGGTTCCTT CCGGTATTGT 
     4741 CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT AGGGTGGGCG AAGAACTCCA GCATGAGATC 
     4801 CCCGCGCTGG AGGATCATCC AGCCGGCGTC CCGGAAAACG ATTCCGAAGC CCAACCTTTC 
     4861 ATAGAAGGCG GCGGTGGAAT CGAAATCTCG TGATGGCAGG TTGGGCGTCG CTTGGTCGGT 
     4921 CATTTCGAAC CCCAGAGTCC CGCTCAGAAG AACTCGTCAA GAAGGCGATA GAAGGCGATG 
     4981 CGCTGCGAAT CGGGAGCGGC GATACCGTAA AGCACGAGGA AGCGGTCAGC CCATTCGCCG 
     5041 CCAAGCTCTT CAGCAATATC ACGGGTAGCC AACGCTATGT CCTGATAGCG ATCCGCCACA 
     5101 CCCAGCCGGC CACAGTCGAT GAATCCAGAA AAGCGGCCAT TTTCCACCAT GATATTCGGC 
     5161 AAGCAGGCAT CGCCATGGGT CACGACGAGA TCCTCGCCGT CGGGCATGCT CGCCTTGAGC 
     5221 CTGGCGAACA GTTCGGCTGG CGCGAGCCCC TGATGCTCTT CGTCCAGATC ATCCTGATCG 
     5281 ACAAGACCGG CTTCCATCCG AGTACGTGCT CGCTCGATGC GATGTTTCGC TTGGTGGTCG 
     5341 AATGGGCAGG TAGCCGGATC AAGCGTATGC AGCCGCCGCA TTGCATCAGC CATGATGGAT 
     5401 ACTTTCTCGG CAGGAGCAAG GTGAGATGAC AGGAGATCCT GCCCCGGCAC TTCGCCCAAT 
     5461 AGCAGCCAGT CCCTTCCCGC TTCAGTGACA ACGTCGAGCA CAGCTGCGCA AGGAACGCCC 
     5521 GTCGTGGCCA GCCACGATAG CCGCGCTGCC TCGTCTTGCA GTTCATTCAG GGCACCGGAC 
     5581 AGGTCGGTCT TGACAAAAAG AACCGGGCGC CCCTGCGCTG ACAGCCGGAA CACGGCGGCA 
     5641 TCAGAGCAGC CGATTGTCTG TTGTGCCCAG TCATAGCCGA ATAGCCTCTC CACCCAAGCG 
     5701 GCCGGAGAAC CTGCGTGCAA TCCATCTTGT TCAATCATGC GAAACGATCC TCATCCTGTC 
     5761 TCTTGATCGA TCTTTGCAAA AGCCTAGGCC TCCAAAAAAG CCTCCTCACT ACTTCTGGAA 
     5821 TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC TGCATAAATA AAAAAAATTA GTCAGCCATG 
     5881 GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG 
     5941 GACTATGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     6001 TGGGGACTTT CCACACCTGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     6061 TGCTGGGGAG CCTGGGGACT TTCCACACCC TAACTGACAC ACATTCCACA GCTGGTTCTT 
     6121 TCCGCCTCAG GACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC 
     6181 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA 
     6241 TTTCCCCGAA AAGTGCCACC TGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG 
     6301 GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT 
     6361 TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG 
     6421 CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG 
     6481 GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG 
     6541 GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC 
     6601 TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT 
     6661 GAGCTGATTT AACAAAAATT TAACGCGAAT TTT
//