LOCUS       RC227339                8514 bp    DNA     circular  SYN 
DEFINITION  GRID2IP (Myc-DDK-tagged)-Human glutamate receptor, ionotropic, delta 2 (Grid2) interacting protein (GRID2IP)
ACCESSION   NM_001145118
VERSION     NM_001145118.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4671..4762 
                     /label="Myc-DDK"  
     misc_feature    4792..5377 
                     /label="PolyA signal"  
     misc_feature    4849..4868 
                     /label="Restriction Enzyme"  
     misc_feature    5653..6325 
                     /label="ColE1"  
     misc_feature    6755..7549 
                     /label="Kan/Neo"  
     misc_feature    7584..7886 
                     /label="SV40 ori"  
     misc_feature    7972..8449 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4653..4659 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4653 
                     /label="GRID2IP"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCCACCACT GCCACGCCGG CCACGAACCA GGGCTGGCCA GAGGACTTTG 
     1081 GCTTCCGGCT AGGTGGCTCT GGCCCCTGCT TCGTCCTGGA GGTGGCCAAG GGGAGCAGCG 
     1141 CGCATGCCGG AGGACTGCGG CCAGGAGACC AGATCCTGGA GGTGGAGGGG CTGGCGGTGG 
     1201 GCGGTCTGAG CCGCGAGCGC CTCGTGCGCC TGGCACGGCG CTGCCCACGT GTGCCGCCCA 
     1261 GTCTGGGCGT GCTCCCGGCT CCCGACGGTG GCCCCGGCCC AGGATCCGGC CCCGCGGCCC 
     1321 CGACCACAGT CTTGCGGGCC CCGCGGTGCG GCCGCGGCCT AGCTCTGGGC CGTGAGCTGC 
     1381 TTCGCCTGGC CGGCCGCAAG CGCCCGGACG CGGTGCACCG AGAGCGCAGG CGCAAGGCCC 
     1441 AAGAGTTCAG CCGCAAGGTG GATGAAATCT TGGGGGACCA GCCAACTGCC AAGGAGCAGG 
     1501 TGTTCGCTGC ACTGAAGCAG TTTGCAGCTG AGCAGCGGGT GGATGATCTG GTGTGGACTC 
     1561 TCACCCTGGC GCTGCCCCGA GAGGCCTGCG GGCCACTCCT GGACAACCTC AGGATCTTCA 
     1621 TCCCCAAGAA GCACCGGGCG CGCTTCGACG AGGTGGTGTC TCAGGGCCTC CTGGGCAAGC 
     1681 TGTGCCGCGC ACGCCGGGCC CAGGGCGCGC AGCGACTGCG CCGGAGCCGC AGCGAGGAGC 
     1741 GGCCGGAGCG CCTCCTGGTG TCCACGCGCG CCAGCGCCCC GCCGCGCCGC CCCGATGAGC 
     1801 CGCCCCCGCG CAGGGCCTCC TTGCTGGTGG GCGGCCTCGC CGGCCCCGGG GGCGCGCGCA 
     1861 GGACTGTCCG AGTCTACAAA GGCAACAAGA GCTTCGGCTT CACACTTCGC GGCCACGGGC 
     1921 CTGTCTGGAT CGAGTCTGTC CTGCCTGGGA GCCCAGCTGA CAATGCTGCC CTCAAGTCAG 
     1981 GTGACCGGAT CCTCTTCCTC AATGGACTGG ACATGAGGAA CTGCTCCCAC GACAAGGTGG 
     2041 TGTCCATGCT GCAGGGCAGT GGTGCCATGC CCACGCTGGT GGTGGAGGAA GGGCTCGTCC 
     2101 CATTTGCCAG TGACTCCGAT TCTCTGGACT CACCCAACCC GTCGTCGGCG CTCACCTCCC 
     2161 TGCAGTGGGT GGCGGAGATC CTGCCGTCCA GCATCCGGGT CCAAGGGAGG ACCTTCAGCC 
     2221 AGCAGCTGGA GCACCTACTC ACACCTCCTG AGCGCTATGG GGTCTGCCGG GCCCTCGAGA 
     2281 GCTTCTTCCA GCACAGGAAC ATCGACACCC TCATCGTTGA CGTCTACCCT GTGCTGGACA 
     2341 CCCCTGCCAA GCAGGTCCTG TGGCAGTTCA TCTACCAGCT GCTGACCTAT GAGGAGCAGG 
     2401 AGCTCTGTCA GGAGAAAATC GCATGCTTCC TGGGCTACAC GGCCATGACA GCAGAGCCGG 
     2461 AGCCTGAGCT GGACCTGGAG TCCGAGCCCA CGCCTGAGCC CCAGCCGCGG AGCTCCCTGC 
     2521 GGGCTTCCTC CATGTGCCGC CGCAGCCTCC GGTCCCAGGG CCTGGAGGCC GGCCTCAGCT 
     2581 GCGGTCCCAG CGAGTGCCCT GAGATGCCTC TTCCCCTGAT CCCAGGCGAG CGCCAGGCAG 
     2641 GCGACGGCAC GTCCCTCCCT GAGACCCCCA ACCCCAAGAT GATGTCAGCC GTCTATGCAG 
     2701 AGCTTGAGTC TCGACTGAAC AGCAGCTTCA AAGGGAAGAT GGGGACCGTG TCCAAATCCC 
     2761 GGGCTTCCCC TCCAGGCCCC AGCCCAGCAG TCACCACAGG GCCCAGGACC CTGTCCGGCG 
     2821 TCTCATGGCC CAGCGAGCGA CTCTTGCCCT CCCCCTGCTA CCACCCGCTG TGTTCGGGGG 
     2881 GTCTGGCCTC CCCCAGCAGC TCTGAGTCCC ACCCCTACGC CAGCCTGGAC AGCAGCAGGG 
     2941 CACCCTCCCC ACAGCCAGGC CCCGGGCCCA TCTGCCCCGA CAGCCCCCCA AGCCCGGACC 
     3001 CCACCCGCCC GCCCAGCCGC AGGAAGCTCT TCACCTTCTC CCACCCTGTG CGAAGCCGCG 
     3061 ATACTGACCG CTTCCTGGAC GTGCTGAGCG AGCAGCTGGG CCCCCGGGTC ACCATCGTGG 
     3121 ATGATTTCCT GACCCCGGAG AACGACTACG AGGAGATGAG CTTCCATGAT GACCAGGGCA 
     3181 GCTTCGTAAC CAATGAGCGG AGCAGCGCCA GCGACTGCAT CAGCAGCAGT GAAGAAGGCA 
     3241 GCTCCCTGAC CTACTCCTCC ATCTCTGACC ACATCCCCCC ACCCCCGCTC AGCCCCCCAC 
     3301 CACCGCCACC CCTGCCTTTC CATGACGCTA AGCCCAGCTC CCGCAGCTCT GATGGTTCCC 
     3361 GGGGCCCTGC TCAGGCGCTG GCCAAGCCCC TCACCCAACT CAGCCACCCA GTCCCTCCAC 
     3421 CACCCCCACC GCCCCTGCCC CCACCCGTGC CCTGTGCACC CCCCATGCTG TCCCGGGGCC 
     3481 TGGGCCACCG GCGCAGTGAG ACCAGCCACA TGAGCGTCAA GCGCTTGCGG TGGGAACAGG 
     3541 TGGAGAACTC AGAAGGCACC ATCTGGGGTC AGCTCGGGGA AGACTCTGAC TACGATAAGC 
     3601 TGAGTGACAT GGTGAAATAC CTCGACCTGG AGCTCCACTT CGGCACCCAG AAACCTGCTA 
     3661 AACCGGTGCC GGGGCCGGAG CCCTTCCGGA AGAAGGAGGT GGTGGAGATC CTGTCCCATA 
     3721 AGAAGGCCTA CAACACCTCC ATCCTCTTGG CACACCTGAA GCTGAGCCCC GCGGAGCTGC 
     3781 GCCAGGTGCT GATGAGCATG GAGCCCCGGC GCCTGGAGCC CGCACATCTC GCGCAGCTGC 
     3841 TGCTCTTCGC GCCCGACGCC GACGAGGAGC AGCGCTACCA GGCCTTCCGC GAGGCGCCCG 
     3901 GCCGCCTCAG CGAGCCGGAC CAGTTCGTCC TGCAGATGCT GTCAGTTCCC GAATACAAGA 
     3961 CACGCCTGCG CAGCCTCCAC TTCCAGGCCA CCCTCCAGGA GAAGACAGAG GAGATCCGAG 
     4021 GCAGCCTTGA ATGCTTGCGC CAGGCCTCCC TGGAGCTCAA AAACAGTCGG AAGCTCGCCA 
     4081 AGATCCTGGA GTTTGTGTTG GCCATGGGCA ACTATCTCAA CGATGGACAG CCCAAAACCA 
     4141 ACAAGACTAC GGGCTTCAAG ATCAACTTTC TGACAGAGCT GAACTCCACC AAGACAGTGG 
     4201 ATGGGAAGTC CACCTTCCTG CACATCCTTG CCAAATCGCT GAGCCAGCAC TTCCCTGAAC 
     4261 TCCTGGGCTT TGCTCAGGAC CTGCCCACCG TGCCCCTGGC TGCCAAAGTG AACCAACGGG 
     4321 CCCTGACCAG TGACCTGGCT GACCTCCATG GCACTATCAG CGAGATACAG GATGCCTGCC 
     4381 AGAGCATTTC CCCCTCTAGC GAGGACAAGT TTGCAATGGT CATGTCGTCC TTCCTGGAGA 
     4441 CGGCCCAGCC AGCACTTCGG GCGCTCGACG GGCTGCAGCG CGAGGCCATG GAGGAGCTGG 
     4501 GCAAGGCGCT GGCCTTCTTT GGGGAGGATT CCAAGGCCAC CACCTCTGAG GCTTTCTTCG 
     4561 GCATCTTTGC AGAGTTCATG AGCAAATTCG AGCGAGCGCT GAGTGACCTG CAGGCCGGGG 
     4621 AGGGCCTGCG CAGCTCCGGG ATGGTTTCAC CCCTGGCCTG GACGCGTACG CGGCCGCTCG 
     4681 AGCAGAAACT CATCTCAGAA GAGGATCTGG CAGCAAATGA TATCCTGGAT TACAAGGATG 
     4741 ACGACGATAA GGTTTAAACG GCCGGCCGCG GTCATAGCTG TTTCCTGAAC AGATCCCGGG 
     4801 TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG 
     4861 CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC 
     4921 TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC 
     4981 TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC 
     5041 ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG 
     5101 GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT 
     5161 TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG 
     5221 CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT 
     5281 TTAAAATAAC TATACCAGCA GGAGGACGTC CAGACACAGC ATAGGCTACC TGGCCATGCC 
     5341 CAACCGGTGG GACATTTGAG TTGCTTGCTT GGCACTGTCC TCTCATGCGT TGGGTCCACT 
     5401 CAGTAGATGC CTGTTGAATT GGGTACGCGG CCAGCGGCGA GCGGTATCAG CTCACTCAAA 
     5461 GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA 
     5521 AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT 
     5581 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 
     5641 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 
     5701 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 
     5761 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 
     5821 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 
     5881 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 
     5941 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 
     6001 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 
     6061 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 
     6121 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC 
     6181 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC 
     6241 AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG 
     6301 TATATATGAG TAACCTGAGG CTATGGCAGG GCCTGCCGCC CCGACGTTGG CTGCGAGCCC 
     6361 TGGGCCTTCA CCCGAACTTG GGGGGTGGGG TGGGGAAAAG GAAGAAACGC GGGCGTATTG 
     6421 GCCCCAATGG GGTCTCGGTG GGGTATCGAC AGAGTGCCAG CCCTGGGACC GAACCCCGCG 
     6481 TTTATGAACA AACGACCCAA CACCGTGCGT TTTATTCTGT CTTTTTATTG CCGTCATAGC 
     6541 GCGGGTTCCT TCCGGTATTG TCTCCTTCCG TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC 
     6601 GAAGAACTCC AGCATGAGAT CCCCGCGCTG GAGGATCATC CAGCCGGCGT CCCGGAAAAC 
     6661 GATTCCGAAG CCCAACCTTT CATAGAAGGC GGCGGTGGAA TCGAAATCTC GTGATGGCAG 
     6721 GTTGGGCGTC GCTTGGTCGG TCATTTCGAA CCCCAGAGTC CCGCTCAGAA GAACTCGTCA 
     6781 AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA TCGGGAGCGG CGATACCGTA AAGCACGAGG 
     6841 AAGCGGTCAG CCCATTCGCC GCCAAGCTCT TCAGCAATAT CACGGGTAGC CAACGCTATG 
     6901 TCCTGATAGC GATCCGCCAC ACCCAGCCGG CCACAGTCGA TGAATCCAGA AAAGCGGCCA 
     6961 TTTTCCACCA TGATATTCGG CAAGCAGGCA TCGCCATGGG TCACGACGAG ATCCTCGCCG 
     7021 TCGGGCATGC TCGCCTTGAG CCTGGCGAAC AGTTCGGCTG GCGCGAGCCC CTGATGCTCT 
     7081 TCGTCCAGAT CATCCTGATC GACAAGACCG GCTTCCATCC GAGTACGTGC TCGCTCGATG 
     7141 CGATGTTTCG CTTGGTGGTC GAATGGGCAG GTAGCCGGAT CAAGCGTATG CAGCCGCCGC 
     7201 ATTGCATCAG CCATGATGGA TACTTTCTCG GCAGGAGCAA GGTGAGATGA CAGGAGATCC 
     7261 TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG TCCCTTCCCG CTTCAGTGAC AACGTCGAGC 
     7321 ACAGCTGCGC AAGGAACGCC CGTCGTGGCC AGCCACGATA GCCGCGCTGC CTCGTCTTGC 
     7381 AGTTCATTCA GGGCACCGGA CAGGTCGGTC TTGACAAAAA GAACCGGGCG CCCCTGCGCT 
     7441 GACAGCCGGA ACACGGCGGC ATCAGAGCAG CCGATTGTCT GTTGTGCCCA GTCATAGCCG 
     7501 AATAGCCTCT CCACCCAAGC GGCCGGAGAA CCTGCGTGCA ATCCATCTTG TTCAATCATG 
     7561 CGAAACGATC CTCATCCTGT CTCTTGATCG ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA 
     7621 GCCTCCTCAC TACTTCTGGA ATAGCTCAGA GGCCGAGGCG GCCTCGGCCT CTGCATAAAT 
     7681 AAAAAAAATT AGTCAGCCAT GGGGCGGAGA ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG 
     7741 GATGGGCGGA GTTAGGGGCG GGACTATGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     7801 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCTG GTTGCTGACT AATTGAGATG 
     7861 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC CTAACTGACA 
     7921 CACATTCCAC AGCTGGTTCT TTCCGCCTCA GGACTCTTCC TTTTTCAATA TTATTGAAGC 
     7981 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA 
     8041 CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC 
     8101 GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC 
     8161 CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC 
     8221 CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC 
     8281 GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG 
     8341 GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT 
     8401 GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT 
     8461 TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
//