LOCUS       RC227074                7725 bp    DNA     circular  SYN 
DEFINITION  CCDC66 (Myc-DDK-tagged)-Human coiled-coil domain containing 66 (CCDC66), transcript variant 1
ACCESSION   NM_001141947
VERSION     NM_001141947.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3882..3973 
                     /label="Myc-DDK"  
     misc_feature    4003..4588 
                     /label="PolyA signal"  
     misc_feature    4060..4079 
                     /label="Restriction Enzyme"  
     misc_feature    4864..5536 
                     /label="ColE1"  
     misc_feature    5966..6760 
                     /label="Kan/Neo"  
     misc_feature    6795..7097 
                     /label="SV40 ori"  
     misc_feature    7183..7660 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3864..3870 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3864 
                     /label="CCDC66"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAACTTGGGA GATGGTTTAA AGCTTGAAAC TGAATTACTG GATGGAAAAA 
     1081 CCAAGCTAAT ATTGTCTCCA TATGAACATA AATCAAAAAT TTCTGTGAAG ATGGGAAATA 
     1141 AGGCCAAGAT TGCAAAATGT CCTTTAAGAA CAAAAACTGG GCACATTCTA AAATCAACAC 
     1201 AAGATACTTG TATTGGGAGT GAAAAACTTT TGCAAAAGAA GCCAGTTGGT TCAGAAACAT 
     1261 CACAGGCAAA AGGTGAAAAA AATGGAATGA CTTTTTCATC CACTAAGGAT TTATGTAAAC 
     1321 AATGTATAGA TAAAGACTGT CTTCATATCC AGAAAGAGAT TTCACCTGCA ACCCCTAATA 
     1381 TGCAGAAGAC TAGAAACACC GTAAATACAT CTCTAGTAGG TAAACAGAAG CCTCACAAAA 
     1441 AACACATCAC AGCTGAAAAC ATGAAGAGCA GTTTGGTGTG TCTAACACAA GACCAACTAC 
     1501 AACAGATTTT GATGACTGTA AACCAAGGAA ATAGATCTCT TTCCCTGACT GAGAATGGAA 
     1561 AGGAGGCAAA AAGTCAATAT AGTCTATATT TAAACAGTAT TTCTAATCAG CCAAAGGATG 
     1621 AGAACATTAT GGGATTATTC AAAAAAACTG AAATGGTTTC ATCTGTCCCA GCTGAAAATA 
     1681 AATCTGTCTT AAATGAACAT CAGGAGACAT CTAAACAGTG TGAGCAAAAA ATTGCCATAG 
     1741 AGAATGAATG GAAACCAGCT GATATATTCA GTACTCTGGG GGAAAGGGAA TGTGATAGAA 
     1801 GTTCGTTGGA AGCAAAAAAA GCCCAGTGGA GGAAAGAGCT AGATGAACAG GTTGCTTTAA 
     1861 AGAAGAAAGA AAAAGAAGTT TCTGAAAAAT GGAATGATCC TTGGAAAAAA TCTGAAAGTG 
     1921 ATAAAATAAT ATGGGAAAAA CATCAAATTC TTGACCAATC TAGGGAAACA GTACTGCTGG 
     1981 AGCACCCTTT CAGTGCTGTG AAACAAGAAC TGCAAAGAAA ATGGATTGAA GAGTTGAATA 
     2041 AGCAAATAGA AGATGACCGT CAAAGAAAAA TAGAGGAAAA AATTATATAT TCAAAGGGTG 
     2101 AGGAACATGA CAGATGGGCA ATGCACTTTG ATTCATTAAA GAGTTATCCT GGTTCTCAAT 
     2161 CTCAGCTGTT CTCTCAGTCA ACACACAAAC AACCTGAGTA CTTCTGTGTC TCTCCTGACA 
     2221 CTCAGGAGCT GGCTGATGTC AGCAGTGTTT GTACACCTAC AACCGGAAGC CAGGTTGAAC 
     2281 CTTCAGAGGA GGAGCATATA GCAAAACCTA TTAAGGATGT GGTTATGGCA AACAGTAAGA 
     2341 AAACAAACTT TCTCCGTTCT ATGACTGCTC TCTTGGACCC AGCTCAGATT GAGGAACGAG 
     2401 ACAGACGACG ACAAAAACAA TTAGAGCATC AGAAAGCCAT CACTGCCCAG GTAGAAGAGA 
     2461 AGCGCAGGAA GAAACAACTG GAGGAAGAGC AAAGAAAGAA GGAAGAACAA GAAGAGGAGC 
     2521 TTCGCTTAGC ACAGGAACGT GAAGAGATGC AGAAACAGTA TGAAGAAGAC ATACTTAAGC 
     2581 AAAAACAAAA GGAAGAAATC ATGACTCTCA AGACAAATGA GCTATTCCAG ACAATGCAGC 
     2641 GAGCACAGGA ACTGGCACAG AGACTAAAAC AAGAACAAAG AATCCGAGAA TTGGCGCAAA 
     2701 AGGGACATGA CACTTCTAGA CTGATTAAAA ATCTTGGTGT TGATACAATA CAAATGGAAT 
     2761 ATAATGCATC TAACATTTCA AATTCAAGAC ATGATTCTGA TGAAATCAGT GGTAAAATGA 
     2821 ATACATATAT GAATTCTACG ACTTCTAAGA AGGATACTGG TGTGCAAACA GATGACTTAA 
     2881 ATATAGGAAT ATTCACCAAT GCAGAATCAC ATTGTGGATC ATTAATGGAG AGGGACATCA 
     2941 CAAATTGTTC ATCTCCTGAG ATTTCGGCAG AACTTATTGG ACAGTTTAGC ACCAAGAAAA 
     3001 ACAAGCAAGA ACTAACTCAG GATAAAGGAG CCAGCTTAGA AAAAGAAAAC AATCGGTGTA 
     3061 ATGACCAGTG TAATCAGTTC ACAAGAATAG AGAAACAAAC AAAACACATG AAGAAATATC 
     3121 CTAAAAGGCC TGATTGGAAT ATAAATAAGC CACCTAAAAG GTATATTCCA GCATCAGAAA 
     3181 AGTACCCTAA ACAGCTTCAA AAGCAGAGAG AAGAAAAAAA AGTAAGGAGG CAGATGGAAT 
     3241 TGCTTCATTT GGTAGAAAAA AATAATCCTG GGCACCTCTC TCAAAACAGA GGCATTTCAC 
     3301 CAGAAATTTT TCATTCATCT CATCAAGAAA CGGAGTCAAA GTTGAGGTGG CATCTAGTCA 
     3361 AAAAGGAAGA AGAGCCTCTG AATATTCATT CATTCAGCAA GGAAAGGTCT CCATCATCAC 
     3421 CAGTTCCAGT AGTGAAAAAC AGAACCCAAC AAACTCAAAA TACATTACAT TTACCACTAA 
     3481 AAAACAGTAG CTATGAGAGA GAGAATTTGA TCTCAGGAAG TAATCAAACA GAATTATCAT 
     3541 CTGGGATTTC TGAATCATCC CATTTTATTC CGTATGTTCG AACAAATGAG ATCTATTACC 
     3601 TTGATCCCGA TGCACCATTG TCTGGGCCTT CAACCCAGGA CCCTCAGTAC CAAAATTCAC 
     3661 AAGACTGTGG CCAAAAACGA CAGCTATTTG ATTCTGACTG TGTCAGGGAT CCACTTCTTA 
     3721 ATCCTAACAT GGTGAAAAAT AGGGATCGAC AGCAAGCAAT CCTTAAGGGA CTTTCAGAAC 
     3781 TGAGACAGGG CCTTCTCCAG AAGCAAAAGG AGTTGGAAAG TAGTCTCCTG CCTTTAGCTG 
     3841 AAAATCAAGA AGAGAGTTTT GGTTCTTCAT TTACGCGTAC GCGGCCGCTC GAGCAGAAAC 
     3901 TCATCTCAGA AGAGGATCTG GCAGCAAATG ATATCCTGGA TTACAAGGAT GACGACGATA 
     3961 AGGTTTAAAC GGCCGGCCGC GGTCATAGCT GTTTCCTGAA CAGATCCCGG GTGGCATCCC 
     4021 TGTGACCCCT CCCCAGTGCC TCTCCTGGCC CTGGAAGTTG CCACTCCAGT GCCCACCAGC 
     4081 CTTGTCCTAA TAAAATTAAG TTGCATCATT TTGTCTGACT AGGTGTCCTT CTATAATATT 
     4141 ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG GGGCAAGTTG GGAAGACAAC CTGTAGGGCC 
     4201 TGCGGGGTCT ATTGGGAACC AAGCTGGAGT GCAGTGGCAC AATCTTGGCT CACTGCAATC 
     4261 TCCGCCTCCT GGGTTCAAGC GATTCTCCTG CCTCAGCCTC CCGAGTTGTT GGGATTCCAG 
     4321 GCATGCATGA CCAGGCTCAG CTAATTTTTG TTTTTTTGGT AGAGACGGGG TTTCACCATA 
     4381 TTGGCCAGGC TGGTCTCCAA CTCCTAATCT CAGGTGATCT ACCCACCTTG GCCTCCCAAA 
     4441 TTGCTGGGAT TACAGGCGTG AACCACTGCT CCCTTCCCTG TCCTTCTGAT TTTAAAATAA 
     4501 CTATACCAGC AGGAGGACGT CCAGACACAG CATAGGCTAC CTGGCCATGC CCAACCGGTG 
     4561 GGACATTTGA GTTGCTTGCT TGGCACTGTC CTCTCATGCG TTGGGTCCAC TCAGTAGATG 
     4621 CCTGTTGAAT TGGGTACGCG GCCAGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT 
     4681 ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA 
     4741 AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC 
     4801 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA 
     4861 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC 
     4921 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC 
     4981 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA 
     5041 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC 
     5101 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG 
     5161 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG 
     5221 AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG 
     5281 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA 
     5341 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA 
     5401 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT 
     5461 CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA 
     5521 GTAACCTGAG GCTATGGCAG GGCCTGCCGC CCCGACGTTG GCTGCGAGCC CTGGGCCTTC 
     5581 ACCCGAACTT GGGGGGTGGG GTGGGGAAAA GGAAGAAACG CGGGCGTATT GGCCCCAATG 
     5641 GGGTCTCGGT GGGGTATCGA CAGAGTGCCA GCCCTGGGAC CGAACCCCGC GTTTATGAAC 
     5701 AAACGACCCA ACACCGTGCG TTTTATTCTG TCTTTTTATT GCCGTCATAG CGCGGGTTCC 
     5761 TTCCGGTATT GTCTCCTTCC GTGTTTCAGT TAGCCTCCCC CTAGGGTGGG CGAAGAACTC 
     5821 CAGCATGAGA TCCCCGCGCT GGAGGATCAT CCAGCCGGCG TCCCGGAAAA CGATTCCGAA 
     5881 GCCCAACCTT TCATAGAAGG CGGCGGTGGA ATCGAAATCT CGTGATGGCA GGTTGGGCGT 
     5941 CGCTTGGTCG GTCATTTCGA ACCCCAGAGT CCCGCTCAGA AGAACTCGTC AAGAAGGCGA 
     6001 TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG GCGATACCGT AAAGCACGAG GAAGCGGTCA 
     6061 GCCCATTCGC CGCCAAGCTC TTCAGCAATA TCACGGGTAG CCAACGCTAT GTCCTGATAG 
     6121 CGATCCGCCA CACCCAGCCG GCCACAGTCG ATGAATCCAG AAAAGCGGCC ATTTTCCACC 
     6181 ATGATATTCG GCAAGCAGGC ATCGCCATGG GTCACGACGA GATCCTCGCC GTCGGGCATG 
     6241 CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT GGCGCGAGCC CCTGATGCTC TTCGTCCAGA 
     6301 TCATCCTGAT CGACAAGACC GGCTTCCATC CGAGTACGTG CTCGCTCGAT GCGATGTTTC 
     6361 GCTTGGTGGT CGAATGGGCA GGTAGCCGGA TCAAGCGTAT GCAGCCGCCG CATTGCATCA 
     6421 GCCATGATGG ATACTTTCTC GGCAGGAGCA AGGTGAGATG ACAGGAGATC CTGCCCCGGC 
     6481 ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC GCTTCAGTGA CAACGTCGAG CACAGCTGCG 
     6541 CAAGGAACGC CCGTCGTGGC CAGCCACGAT AGCCGCGCTG CCTCGTCTTG CAGTTCATTC 
     6601 AGGGCACCGG ACAGGTCGGT CTTGACAAAA AGAACCGGGC GCCCCTGCGC TGACAGCCGG 
     6661 AACACGGCGG CATCAGAGCA GCCGATTGTC TGTTGTGCCC AGTCATAGCC GAATAGCCTC 
     6721 TCCACCCAAG CGGCCGGAGA ACCTGCGTGC AATCCATCTT GTTCAATCAT GCGAAACGAT 
     6781 CCTCATCCTG TCTCTTGATC GATCTTTGCA AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA 
     6841 CTACTTCTGG AATAGCTCAG AGGCCGAGGC GGCCTCGGCC TCTGCATAAA TAAAAAAAAT 
     6901 TAGTCAGCCA TGGGGCGGAG AATGGGCGGA ACTGGGCGGA GTTAGGGGCG GGATGGGCGG 
     6961 AGTTAGGGGC GGGACTATGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     7021 TGCTGGGGAG CCTGGGGACT TTCCACACCT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     7081 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CCTAACTGAC ACACATTCCA 
     7141 CAGCTGGTTC TTTCCGCCTC AGGACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG 
     7201 GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG 
     7261 GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGCGC CCTGTAGCGG CGCATTAAGC 
     7321 GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC 
     7381 GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT 
     7441 CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA 
     7501 AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC 
     7561 CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA 
     7621 CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT 
     7681 TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTT
//