LOCUS       RC227016                8277 bp    DNA     circular  SYN 
DEFINITION  SCAF4 (Myc-DDK-tagged)-Human SR-related CTD-associated factor 4 (SCAF4), transcript variant 2
ACCESSION   NM_001145444
VERSION     NM_001145444.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4434..4525 
                     /label="Myc-DDK"  
     misc_feature    4555..5140 
                     /label="PolyA signal"  
     misc_feature    4612..4631 
                     /label="Restriction Enzyme"  
     misc_feature    5416..6088 
                     /label="ColE1"  
     misc_feature    6518..7312 
                     /label="Kan/Neo"  
     misc_feature    7347..7649 
                     /label="SV40 ori"  
     misc_feature    7735..8212 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4416..4422 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4416 
                     /label="SCAF4"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGACGCCGTC AACGCCTTCA ACCAGGAGCT CTTTTCGCTT ATGGATATGA 
     1081 AACCTCCCAT CTCTAGAGCC AAGATGATTC TCATCACTAA AGCTGCTATT AAAGCTATTA 
     1141 AGTGTAAACC AGAATACAAG GTTCCGGGAT TATATGTAAT TGACTCAATT GTGCGACAGT 
     1201 CTCGTCATCA GTTTGGAACT GATAAAGATG TTTTTGGGCC AAGATTCTCT AAAAACATAA 
     1261 CTGCCACATT CCAATATTTA TATCTTTGTC CATCTGAAGA TAAGAGTAAA ATAGTTCGTG 
     1321 TGCTGAACCT TTGGCAAAAA AATGGAGTGT TCAAAATTGA AATTATTCAA CCTCTTTTGG 
     1381 ACATGGCAGC GGGAACCAGT AATGCAGCCC CAGTAGCAGA AAATGTTACC AATAATGAAG 
     1441 GCTCACCTCC ACCTCCAGTA AAAGTTTCTT CTGAACCTCC CACACAAGCC ACTCCAAACT 
     1501 CCGTCCCAGC TGTACCACAG TTGCCCAGCT CTGATGCTTT TGCTGCTGTG GCTCAGCTGT 
     1561 TTCAGACAAC TCAAGGCCAA CAGCTTCAGC AGATCCTTCA GACTTTTCAA CAGCCTCCAA 
     1621 AACCACAGTC TCCTGCCCTT GACAATGCTG TGATGGCTCA GGTTCAGGCT ATCACAGCTC 
     1681 AGTTAAAGAC AACTCCTACA CAACCATCTG AACAAAAAGC TGCTTTCCCC CCACCTGAAC 
     1741 AGAAAACTGC ATTTGACAAG AAGTTGCTTG ATAGATTTGA CTATGATGAT GAGCCAGAAG 
     1801 CTGTGGAAGA ATCAAAGAAA GAGGATACCA CTGCCGTCAC CACGACAGCA CCTGCTGCCG 
     1861 CAGTACCCCC TGCACCCACC GCCACCGTGC CTGCTGCTGC TGCACCCGCT GCTGCCTCTC 
     1921 CTCCTCCTCC ACAGGCACCA TTTGGCTTTC CTGGAGATGG CATGCAGCAG CCAGCATACA 
     1981 CACAGCATCA AAATATGGAT CAGTTTCAGC CACGAATGAT GGGAATACAA CAGGATCCAA 
     2041 TGCACCATCA GGTTCCACTT CCTCCTAATG GACAAATGCC AGGATTTGGA CTTCTTCCTA 
     2101 CACCTCCATT TCCTCCCATG GCTCAGCCTG TGATTCCTCC AACTCCACCA GTGCAGCAGC 
     2161 CTTTCCAAGC TTCTTTTCAG GCACAAAATG AACCACTTAC ACAGAAGCCG CATCAGCAGG 
     2221 AAATGGAAGT AGAACAACCT TGTATTCAAG AGGTTAAGCG ACATATGTCT GATAACAGAA 
     2281 AGTCAAGATC TAGGTCAGCA TCCAGGTCAC CAAAAAGGAG GCGATCTAGA TCTGGTTCTA 
     2341 GATCTCGAAG GTCTCGGCAT CGACGTTCTC GATCTCGGTC CAGGGATAGA CGCCGACATT 
     2401 CTCCCCGATC TCGATCTCAA GAAAGACGGG ATCGAGAAAA AGAGAGAGAA CGTCGACAAA 
     2461 AAGGCCTCCC TCAAGTGAAA CCGGAAACTG CAAGTGTTTG CAGTACTACC CTCTGGGTGG 
     2521 GGCAGCTGGA CAAAAGAACT ACTCAGCAGG ATGTTGCCAG TCTCTTGGAA GAGTTTGGTC 
     2581 CAATTGAATC AATTAATATG ATTCCTCCCA GGGGTTGTGC CTATATTGTT ATGGTTCATA 
     2641 GGCAAGATGC CTATCGTGCC CTGCAGAAAC TGAGCCGAGG AAACTATAAA GTGAACCAGA 
     2701 AATCCATAAA GATTGCCTGG GCCTTAAACA AAGGAATAAA GGCAGATTAT AAGCAGTATT 
     2761 GGGATGTAGA ACTTGGTGTT ACTTATATTC CATGGGACAA AGTCAAGCCT GAGGAACTGG 
     2821 AGAGTTTTTG TGAAGGAGGA ATGTTGGACA GTGACACACT TAACCCAGAT TGGAAAGGAA 
     2881 TTCCTAAGAA GCCTGAAAAT GAAGTTGCTC AAAATGGAGG TGCTGAAACC TCACACACAG 
     2941 AACCAGTATC ACCCATACCT AAACCATTAC CTGTGCCTGT CCCTCCTATT CCTGTTCCTG 
     3001 CACCTATAAC AGTGCCACCT CCACAGGTCC CACCACATCA ACCGGGTCCA CCTGTAGTTG 
     3061 GTGCTCTCCA GCCGCCTGCT TTCACGCCTC CTCTGGGAAT ACCGCCTCCA GGCTTTGGTC 
     3121 CTGGTGTTCC TCCTCCCCCT CCTCCTCCAC CATTTTTGCG CCCAGGATTC AACCCAATGC 
     3181 ATTTACCACC AGGTTTTCTG CCTCCTGGAC CCCCACCTCC TATAACTCCA CCAGTATCCA 
     3241 TTCCTCCTCC TCACACTCCA CCAATAAGCA TCCCAAACTC TACTATCGCT GGTATAAATG 
     3301 AAGACACTAC AAAAGACTTA TCTATTGGAA ATCCCATTCC AACAGTGGTG TCTGGGGCTA 
     3361 GAGGAAACGC CGAGTCTGGT GACAGCGTGA AAATGTATGG CTCTGCCGTG CCACCTGCTG 
     3421 CACCCACGAA TCTGCCCACC CCTCCTGTAA CCCAGCCTGT TTCACTTCTT GGCACTCAAG 
     3481 GAGTTGCCCC TGGTCCTGTA ATTGGACTTC AGGCACCATC TACTGGTCTT CTTGGCGCCC 
     3541 GGCCCGGTCT CATCCCACTC CAGCGCCCTC CAGGAATGCC CCCACCTCAC TTACAGCGGT 
     3601 TCCCTTTGAT GCCGCCCCGT CCCATGCCAC CGCACATGAT GCACAGAGGC CCACCGCCAG 
     3661 GACCAGGGGG CTTTGCGATG CCTCCACCTC ATGGAATGAA AGGTCCCTTC CCACCGCATG 
     3721 GCCCCTTTGT TAGGCCTGGT GGAATGCCAG GGCTCGGGGG CCCAGGGCCA GGCCCAGGGG 
     3781 GTCCTGAAGA CAGAGACGGA AGGCAACAGC CGCCGCAGCA GCCACAGCAG CAGCCACAGC 
     3841 CGCAGGCGCC CCAGCAACCA CAGCAGCAGC AGCAGCAGCA GCCACCACCA TCACAACAGC 
     3901 CTCCACCAAC ACAGCAGCAG CCACAGCAGT TTAGAAATGA TAACAGGCAG CAGTTCAATT 
     3961 CAGGTAGAGA CCAAGAAAGG TTTGGAAGAA GATCTTTTGG AAATAGGGTG GAAAATGACC 
     4021 GGGAACGGTA TGGGAACCGT AATGATGATA GAGATAATAG TAACCGTGAC AGGAGAGAGT 
     4081 GGGGAAGGAG GAGCCCTGAC CGGGACAGGC ACAGAGACTT GGAAGAGAGA AATAGACGCT 
     4141 CTAGTGGGCA TCGAGACAGA GAGAGAGATT CTAGAGATAG AGAGTCTCGT AGAGAGAAGG 
     4201 AAGAAGCCCG AGGAAAGGAA AAGCCTGAGG TGACAGACAG GGCAGGTGGT AACAAAACCG 
     4261 TTGAACCTCC CATTAGCCAA GTGGGAAATG TAGACACTGC TTCAGAACTT GAGAAGGGGG 
     4321 TGTCTGAGGC TGCAGTCCTA AAGCCTTCTG AAGAGTTACC TGCTGAGGCT ACCTCATCCG 
     4381 TTGAACCCGA AAAGGATTCT GGCTCAGCAG CAGAGGCTCC TCGTACGCGT ACGCGGCCGC 
     4441 TCGAGCAGAA ACTCATCTCA GAAGAGGATC TGGCAGCAAA TGATATCCTG GATTACAAGG 
     4501 ATGACGACGA TAAGGTTTAA ACGGCCGGCC GCGGTCATAG CTGTTTCCTG AACAGATCCC 
     4561 GGGTGGCATC CCTGTGACCC CTCCCCAGTG CCTCTCCTGG CCCTGGAAGT TGCCACTCCA 
     4621 GTGCCCACCA GCCTTGTCCT AATAAAATTA AGTTGCATCA TTTTGTCTGA CTAGGTGTCC 
     4681 TTCTATAATA TTATGGGGTG GAGGGGGGTG GTATGGAGCA AGGGGCAAGT TGGGAAGACA 
     4741 ACCTGTAGGG CCTGCGGGGT CTATTGGGAA CCAAGCTGGA GTGCAGTGGC ACAATCTTGG 
     4801 CTCACTGCAA TCTCCGCCTC CTGGGTTCAA GCGATTCTCC TGCCTCAGCC TCCCGAGTTG 
     4861 TTGGGATTCC AGGCATGCAT GACCAGGCTC AGCTAATTTT TGTTTTTTTG GTAGAGACGG 
     4921 GGTTTCACCA TATTGGCCAG GCTGGTCTCC AACTCCTAAT CTCAGGTGAT CTACCCACCT 
     4981 TGGCCTCCCA AATTGCTGGG ATTACAGGCG TGAACCACTG CTCCCTTCCC TGTCCTTCTG 
     5041 ATTTTAAAAT AACTATACCA GCAGGAGGAC GTCCAGACAC AGCATAGGCT ACCTGGCCAT 
     5101 GCCCAACCGG TGGGACATTT GAGTTGCTTG CTTGGCACTG TCCTCTCATG CGTTGGGTCC 
     5161 ACTCAGTAGA TGCCTGTTGA ATTGGGTACG CGGCCAGCGG CGAGCGGTAT CAGCTCACTC 
     5221 AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC 
     5281 AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG 
     5341 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC 
     5401 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT 
     5461 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT 
     5521 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG 
     5581 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT 
     5641 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT 
     5701 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG 
     5761 CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA 
     5821 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT 
     5881 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC 
     5941 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT 
     6001 ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA 
     6061 AAGTATATAT GAGTAACCTG AGGCTATGGC AGGGCCTGCC GCCCCGACGT TGGCTGCGAG 
     6121 CCCTGGGCCT TCACCCGAAC TTGGGGGGTG GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA 
     6181 TTGGCCCCAA TGGGGTCTCG GTGGGGTATC GACAGAGTGC CAGCCCTGGG ACCGAACCCC 
     6241 GCGTTTATGA ACAAACGACC CAACACCGTG CGTTTTATTC TGTCTTTTTA TTGCCGTCAT 
     6301 AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG 
     6361 GGCGAAGAAC TCCAGCATGA GATCCCCGCG CTGGAGGATC ATCCAGCCGG CGTCCCGGAA 
     6421 AACGATTCCG AAGCCCAACC TTTCATAGAA GGCGGCGGTG GAATCGAAAT CTCGTGATGG 
     6481 CAGGTTGGGC GTCGCTTGGT CGGTCATTTC GAACCCCAGA GTCCCGCTCA GAAGAACTCG 
     6541 TCAAGAAGGC GATAGAAGGC GATGCGCTGC GAATCGGGAG CGGCGATACC GTAAAGCACG 
     6601 AGGAAGCGGT CAGCCCATTC GCCGCCAAGC TCTTCAGCAA TATCACGGGT AGCCAACGCT 
     6661 ATGTCCTGAT AGCGATCCGC CACACCCAGC CGGCCACAGT CGATGAATCC AGAAAAGCGG 
     6721 CCATTTTCCA CCATGATATT CGGCAAGCAG GCATCGCCAT GGGTCACGAC GAGATCCTCG 
     6781 CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG AACAGTTCGG CTGGCGCGAG CCCCTGATGC 
     6841 TCTTCGTCCA GATCATCCTG ATCGACAAGA CCGGCTTCCA TCCGAGTACG TGCTCGCTCG 
     6901 ATGCGATGTT TCGCTTGGTG GTCGAATGGG CAGGTAGCCG GATCAAGCGT ATGCAGCCGC 
     6961 CGCATTGCAT CAGCCATGAT GGATACTTTC TCGGCAGGAG CAAGGTGAGA TGACAGGAGA 
     7021 TCCTGCCCCG GCACTTCGCC CAATAGCAGC CAGTCCCTTC CCGCTTCAGT GACAACGTCG 
     7081 AGCACAGCTG CGCAAGGAAC GCCCGTCGTG GCCAGCCACG ATAGCCGCGC TGCCTCGTCT 
     7141 TGCAGTTCAT TCAGGGCACC GGACAGGTCG GTCTTGACAA AAAGAACCGG GCGCCCCTGC 
     7201 GCTGACAGCC GGAACACGGC GGCATCAGAG CAGCCGATTG TCTGTTGTGC CCAGTCATAG 
     7261 CCGAATAGCC TCTCCACCCA AGCGGCCGGA GAACCTGCGT GCAATCCATC TTGTTCAATC 
     7321 ATGCGAAACG ATCCTCATCC TGTCTCTTGA TCGATCTTTG CAAAAGCCTA GGCCTCCAAA 
     7381 AAAGCCTCCT CACTACTTCT GGAATAGCTC AGAGGCCGAG GCGGCCTCGG CCTCTGCATA 
     7441 AATAAAAAAA ATTAGTCAGC CATGGGGCGG AGAATGGGCG GAACTGGGCG GAGTTAGGGG 
     7501 CGGGATGGGC GGAGTTAGGG GCGGGACTAT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     7561 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CTGGTTGCTG ACTAATTGAG 
     7621 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCCTAACTG 
     7681 ACACACATTC CACAGCTGGT TCTTTCCGCC TCAGGACTCT TCCTTTTTCA ATATTATTGA 
     7741 AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT 
     7801 AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGC GCCCTGTAGC 
     7861 GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC 
     7921 GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT 
     7981 CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC 
     8041 CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG 
     8101 ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA 
     8161 ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG 
     8221 ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTT
//