LOCUS       RC226960                8118 bp    DNA     circular  SYN 
DEFINITION  GPR113 (Myc-DDK-tagged)-Human G protein-coupled receptor 113 (GPR113), transcript variant 1
ACCESSION   NM_001145168
VERSION     NM_001145168.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4275..4366 
                     /label="Myc-DDK"  
     misc_feature    4396..4981 
                     /label="PolyA signal"  
     misc_feature    4453..4472 
                     /label="Restriction Enzyme"  
     misc_feature    5257..5929 
                     /label="ColE1"  
     misc_feature    6359..7153 
                     /label="Kan/Neo"  
     misc_feature    7188..7490 
                     /label="SV40 ori"  
     misc_feature    7576..8053 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4257..4263 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4257 
                     /label="ADGRF3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGTCTGTTCG GCTGCCCCAC TGCTGCTCCT GGCCACAACT CTTCCCCTGC 
     1081 TGGGGTCACC AGTTGCCCAA GCATCCCAAC CTGTAAGTGA GACTGGGGTG AGACCCAGGG 
     1141 AAGGTCTGCA GAGGCGACAA TGGGGACCCC TGATTGGGAG AGACAAAGCA TGGAATGAAA 
     1201 GGATAGACAG ACCCTTCCCT GCCTGCCCCA TCCCCCTATC TTCTAGCTTT GGCCGATGGC 
     1261 CCAAGGGCCA GACAATGTGG GCCCAGACCT CCACCCTCAC CCTGACAGAG GAGGAGTTGG 
     1321 GACAGAGTCA GGCTGGAGGG GAATCTGGAT CTGGGCAGCT CCTGGACCAA GAGAATGGAG 
     1381 CAGGGGAATC AGCGCTGGTC TCCGTCTATG TACATCTGGA CTTTCCAGAT AAGACCTGGC 
     1441 CCCCTGAACT CTCCAGGACA CTGACTCTCC CTGCTGCCTC AGCTTCCTCT TCCCCAAGGC 
     1501 CTCTTCTCAC TGGCCTCAGA CTCACAACAG AGTGTAATGT CAACCACAAG GGGAATTTCT 
     1561 ATTGTGCTTG CCTCTCTGGC TACCAGTGGA ACACCAGCAT CTGCCTCCAT TACCCTCCTT 
     1621 GTCAAAGCCT CCACAACCAC CAGCCTTGTG GCTGCCTTGT CTTCAGCCAT CCCGAACCCG 
     1681 GGTACTGCCA GTTGCTGCCA CCTGGGTCCC CTGTCACCTG CCTCCCTGCA GTCCCCGGGA 
     1741 TCCTCAACCT GAACTCCCAG CTGCAGATGC CTGGTGACAC GCTGAGCCTG ACTCTCCATC 
     1801 TGAGCCAGGA GGCCACCAAC CTGAGCTGGT TCCTGAGGCA CCCAGGGAGC CCCAGTCCCA 
     1861 TCCTCCTGCA GCCAGGGACA CAGGTGTCTG TGACTTCCAG CCACGGCCAG GCTGCCCTCA 
     1921 GCGTCTCCAA CATGTCCCAT CACTGGGCAG GTGAGTACAT GAGCTGCTTC GAGGCCCAGG 
     1981 GCTTCAAGTG GAACCTGTAT GAGGTGGTGA GGGTGCCCTT GAAGGCGACA GATGTGGCTC 
     2041 GACTTCCATA CCAGCTGTCC ATCTCCTGTG CCACCTCCCC TGGCTTCCAG CTGAGCTGCT 
     2101 GCATCCCCAG CACAAACCTG GCCTACACCG CGGCCTGGAG CCCTGGAGAG GGCAGCAAAG 
     2161 CTTCCTCCTT CAACGAGTCA GGCTCTCAGT GCTTTGTGCT GGCTGTTCAG CGCTGCCCGA 
     2221 TGGCTGACAC CACGTACGCT TGTGACCTGC AGAGCCTGGG CCTGGCTCCA CTCAGGGTCC 
     2281 CCATCTCCAT CACCATCATC CAGGATGGAG ACATCACCTG CCCTGAGGAC GCCTCGGTGC 
     2341 TCACCTGGAA TGTCACCAAG GCTGGCCACG TGGCACAGGC CCCATGTCCT GAGAGCAAGA 
     2401 GGGGCATAGT GAGGAGGCTC TGTGGGGCTG ACGGAGTCTG GGGGCCGGTC CACAGCAGCT 
     2461 GCACAGATGC GAGGCTCCTG GCCTTGTTCA CTAGAACCAA GCTGCTGCAG GCAGGCCAGG 
     2521 GCAGTCCTGC TGAGGAGGTG CCACAGATCC TGGCACAGCT GCCAGGGCAG GCGGCAGAGG 
     2581 CAAGTTCACC CTCCGACTTA CTGACCCTGC TGAGCACCAT GAAATACGTG GCCAAGGTGG 
     2641 TGGCAGAGGC CAGAATACAG CTTGACCGCA GAGCCCTGAA GAATCTCCTG ATTGCCACAG 
     2701 ACAAGGTCCT AGATATGGAC ACCAGGTCTC TGTGGACCCT GGCCCAAGCC CGGAAGCCCT 
     2761 GGGCAGGCTC GACTCTCCTG CTGGCTGTGG AGACCCTGGC ATGCAGCCTG TGCCCACAGG 
     2821 ACCACCCCTT CGCCTTCAGC TTACCCAATG TGCTGCTGCA GAGCCAGCTG TTTGGACCCA 
     2881 CGTTTCCTGC TGACTACAGC ATCTCCTTCC CTACTCGGCC CCCACTGCAG GCTCAGATTC 
     2941 CCAGGCACTC ACTGGCCCCA TTGGTCCGTA ATGGAACTGA AATAAGTATT ACTAGCCTGG 
     3001 TGCTGCGAAA ACTGGACCAC CTTCTGCCCT CAAACTATGG ACAAGGGCTG GGGGATTCCC 
     3061 TCTATGCCAC TCCTGGCCTG GTCCTTGTCA TTTCCATCAT GGCAGGTGAC CGGGCCTTCA 
     3121 GCCAGGGAGA GGTCATCATG GACTTTGGGA ACACAGATGG TTCCCCTCAC TGTGTCTTCT 
     3181 GGGATCACAG TCTCTTCCAG GGCAGGGGGG GTTGGTCCAA AGAAGGGTGC CAGGCACAGG 
     3241 TGGCCAGTGC CAGCCCCACT GCTCAGTGCC TCTGCCAGCA CCTCACTGCC TTCTCCGTCC 
     3301 TCATGTCCCC ACACACTGTT CCGGAAGAAC CCGCTCTGGC GCTGCTGACT CAAGTGGGCT 
     3361 TGGGAGCTTC CATACTGGCG CTGCTTGTGT GCCTGGGTGT GTACTGGCTG GTGTGGAGAG 
     3421 TCGTGGTGCG GAACAAGATC TCCTATTTCC GCCACGCCGC CCTGCTCAAC ATGGTGTTCT 
     3481 GCTTGCTGGC CGCAGACACT TGCTTCCTGG GCGCCCCATT CCTCTCTCCA GGGCCCCGAA 
     3541 GCCCGCTCTG CCTTGCTGCC GCCTTCCTCT GTCATTTCCT CTACCTGGCC ACCTTTTTCT 
     3601 GGATGCTGGC GCAGGCCCTG GTGTTGGCCC ACCAGCTGCT CTTTGTCTTT CACCAGCTGG 
     3661 CAAAGCACCG AGTTCTCCCC CTCATGGTGC TCCTGGGCTA CCTGTGCCCA CTGGGGTTGG 
     3721 CAGGTGTCAC CCTGGGGCTC TACCTACCTC AAGGGCAATA CCTGAGGGAG GGGGAATGCT 
     3781 GGTTGGATGG GAAGGGAGGG GCGTTATACA CCTTCGTGGG GCCAGTGCTG GCCATCATAG 
     3841 GCGTGAATGG GCTGGTACTA GCCATGGCCA TGCTGAAGTT GCTGAGACCT TCGCTGTCAG 
     3901 AGGGACCCCC AGCAGAGAAG CGCCAAGCTC TGCTGGGGGT GATCAAAGCC CTGCTCATTC 
     3961 TTACACCCAT CTTTGGCCTC ACCTGGGGGC TGGGCCTGGC CACTCTGTTA GAGGAAGTCT 
     4021 CCACGGTCCC TCATTACATC TTCACCATTC TCAACACCCT CCAGGGCGTC TTCATCCTAT 
     4081 TGTTTGGTTG CCTCATGGAC AGGAAGATAC AAGAAGCTTT GCGCAAACGC TTCTGCCGCG 
     4141 CCCAAGCCCC CAGCTCCACC ATCTCCCTGG TGAGTTGCTG CCTTCAGATC CTCAGCTGTG 
     4201 CATCCAAGAG CATGTCAGAA GGCATTCCAT GGCCCTCCTC AGAGGACATG GGCACAGCCA 
     4261 GAAGCACGCG TACGCGGCCG CTCGAGCAGA AACTCATCTC AGAAGAGGAT CTGGCAGCAA 
     4321 ATGATATCCT GGATTACAAG GATGACGACG ATAAGGTTTA AACGGCCGGC CGCGGTCATA 
     4381 GCTGTTTCCT GAACAGATCC CGGGTGGCAT CCCTGTGACC CCTCCCCAGT GCCTCTCCTG 
     4441 GCCCTGGAAG TTGCCACTCC AGTGCCCACC AGCCTTGTCC TAATAAAATT AAGTTGCATC 
     4501 ATTTTGTCTG ACTAGGTGTC CTTCTATAAT ATTATGGGGT GGAGGGGGGT GGTATGGAGC 
     4561 AAGGGGCAAG TTGGGAAGAC AACCTGTAGG GCCTGCGGGG TCTATTGGGA ACCAAGCTGG 
     4621 AGTGCAGTGG CACAATCTTG GCTCACTGCA ATCTCCGCCT CCTGGGTTCA AGCGATTCTC 
     4681 CTGCCTCAGC CTCCCGAGTT GTTGGGATTC CAGGCATGCA TGACCAGGCT CAGCTAATTT 
     4741 TTGTTTTTTT GGTAGAGACG GGGTTTCACC ATATTGGCCA GGCTGGTCTC CAACTCCTAA 
     4801 TCTCAGGTGA TCTACCCACC TTGGCCTCCC AAATTGCTGG GATTACAGGC GTGAACCACT 
     4861 GCTCCCTTCC CTGTCCTTCT GATTTTAAAA TAACTATACC AGCAGGAGGA CGTCCAGACA 
     4921 CAGCATAGGC TACCTGGCCA TGCCCAACCG GTGGGACATT TGAGTTGCTT GCTTGGCACT 
     4981 GTCCTCTCAT GCGTTGGGTC CACTCAGTAG ATGCCTGTTG AATTGGGTAC GCGGCCAGCG 
     5041 GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA 
     5101 CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC 
     5161 GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC 
     5221 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG 
     5281 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT 
     5341 CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA 
     5401 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC 
     5461 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC 
     5521 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT 
     5581 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT 
     5641 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC 
     5701 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA 
     5761 AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 
     5821 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 
     5881 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAACCT GAGGCTATGG CAGGGCCTGC 
     5941 CGCCCCGACG TTGGCTGCGA GCCCTGGGCC TTCACCCGAA CTTGGGGGGT GGGGTGGGGA 
     6001 AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA ATGGGGTCTC GGTGGGGTAT CGACAGAGTG 
     6061 CCAGCCCTGG GACCGAACCC CGCGTTTATG AACAAACGAC CCAACACCGT GCGTTTTATT 
     6121 CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC 
     6181 AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA CTCCAGCATG AGATCCCCGC GCTGGAGGAT 
     6241 CATCCAGCCG GCGTCCCGGA AAACGATTCC GAAGCCCAAC CTTTCATAGA AGGCGGCGGT 
     6301 GGAATCGAAA TCTCGTGATG GCAGGTTGGG CGTCGCTTGG TCGGTCATTT CGAACCCCAG 
     6361 AGTCCCGCTC AGAAGAACTC GTCAAGAAGG CGATAGAAGG CGATGCGCTG CGAATCGGGA 
     6421 GCGGCGATAC CGTAAAGCAC GAGGAAGCGG TCAGCCCATT CGCCGCCAAG CTCTTCAGCA 
     6481 ATATCACGGG TAGCCAACGC TATGTCCTGA TAGCGATCCG CCACACCCAG CCGGCCACAG 
     6541 TCGATGAATC CAGAAAAGCG GCCATTTTCC ACCATGATAT TCGGCAAGCA GGCATCGCCA 
     6601 TGGGTCACGA CGAGATCCTC GCCGTCGGGC ATGCTCGCCT TGAGCCTGGC GAACAGTTCG 
     6661 GCTGGCGCGA GCCCCTGATG CTCTTCGTCC AGATCATCCT GATCGACAAG ACCGGCTTCC 
     6721 ATCCGAGTAC GTGCTCGCTC GATGCGATGT TTCGCTTGGT GGTCGAATGG GCAGGTAGCC 
     6781 GGATCAAGCG TATGCAGCCG CCGCATTGCA TCAGCCATGA TGGATACTTT CTCGGCAGGA 
     6841 GCAAGGTGAG ATGACAGGAG ATCCTGCCCC GGCACTTCGC CCAATAGCAG CCAGTCCCTT 
     6901 CCCGCTTCAG TGACAACGTC GAGCACAGCT GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC 
     6961 GATAGCCGCG CTGCCTCGTC TTGCAGTTCA TTCAGGGCAC CGGACAGGTC GGTCTTGACA 
     7021 AAAAGAACCG GGCGCCCCTG CGCTGACAGC CGGAACACGG CGGCATCAGA GCAGCCGATT 
     7081 GTCTGTTGTG CCCAGTCATA GCCGAATAGC CTCTCCACCC AAGCGGCCGG AGAACCTGCG 
     7141 TGCAATCCAT CTTGTTCAAT CATGCGAAAC GATCCTCATC CTGTCTCTTG ATCGATCTTT 
     7201 GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC TCACTACTTC TGGAATAGCT CAGAGGCCGA 
     7261 GGCGGCCTCG GCCTCTGCAT AAATAAAAAA AATTAGTCAG CCATGGGGCG GAGAATGGGC 
     7321 GGAACTGGGC GGAGTTAGGG GCGGGATGGG CGGAGTTAGG GGCGGGACTA TGGTTGCTGA 
     7381 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     7441 CCTGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     7501 GGACTTTCCA CACCCTAACT GACACACATT CCACAGCTGG TTCTTTCCGC CTCAGGACTC 
     7561 TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA 
     7621 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG 
     7681 CCACCTGACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC 
     7741 GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT 
     7801 CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC 
     7861 CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT 
     7921 AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT 
     7981 AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT 
     8041 GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA 
     8101 AAATTTAACG CGAATTTT
//