LOCUS       RC226873                8109 bp    DNA     circular  SYN 
DEFINITION  RGSL1 (Myc-DDK-tagged)-Human regulator of G-protein signaling like 1 (RGSL1)
ACCESSION   NM_001137669
VERSION     NM_001137669.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4266..4357 
                     /label="Myc-DDK"  
     misc_feature    4387..4972 
                     /label="PolyA signal"  
     misc_feature    4444..4463 
                     /label="Restriction Enzyme"  
     misc_feature    5248..5920 
                     /label="ColE1"  
     misc_feature    6350..7144 
                     /label="Kan/Neo"  
     misc_feature    7179..7481 
                     /label="SV40 ori"  
     misc_feature    7567..8044 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4248..4254 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4248 
                     /label="RGSL1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAGCAGTGCT GAGATAATTG GTTCTACAAA TCTTATAATT CTGCTAGAGG 
     1081 ATGAAGTCTT TGCCGATTTT TTCAACACAT TTCTTTCCCT CCCGGTTTTT GGTCAGACAC 
     1141 CATTTTATAC TGTTGAAAAT TCACAGTGGA GCTTGTGGCC AGAAATACCT TGTAACTTGA 
     1201 TTGCCAAATA CAAAGGGTTA TTGACCTGGT TGGAAAAATG CCGATTACCT TTCTTCTGTA 
     1261 AAACAAACTT GTGTTTCCAT TACATTCTCT GTCAGGAGTT CATCAGTTTC ATTAAGTCCC 
     1321 CAGAAGGAGG TGAAGAATTG GTGGATTTCT GGATCCTTGC TGAGAACATC CTGAGCATAG 
     1381 ATGAGATGGA CCTGGAAGTG AGAGACTACT ACCTGTCCCT CCTCCTCATG CTGAGGGCCA 
     1441 CTCATCTGCA GGAGGGCTCC AGGGTGGTAA CCCTCTGTAA CATGAACATC AAGTCCCTCC 
     1501 TGAACCTCTC CATCTGGCAT CCCAACCAAT CAACCACTAG GAGGGAGATC CTGAGCCACA 
     1561 TGCAGAAAGT GGCTCTGTTC AAACTCCAGA GCTATTGGCT TCCCAACTTT TACACCCACA 
     1621 CCAAGATGAC CATGGCCAAG GAGGAAGCAT GCCATGGTCT GATGCAAGAG TACGAGACTC 
     1681 GCTTATACAG CGTTTGCTAC ACCCACATAG GAGGGCTCCC TCTGAACATG AGCATCAAGA 
     1741 AGTGCCACCA CTTTCAGAAA CGGTACTCAA GCAGGAAAGC CAAGAGGAAG ATGTGGCAAT 
     1801 TGGTAGATCC TGACTCTTGG TCTCTGGAAA TGGATCTCAA GCCAGATGCT ATTGGTATGC 
     1861 CCCTACAGGA GACATGTCCT CAAGAGAAGG TGGTTATACA AATGCCTTCC CTGAAAATGG 
     1921 CTTCTTCAAA GGAAACAAGA ATCAGTTCCC TGGAAAAGGA TATGCATTAT GCAAAAATAT 
     1981 CCAGCATGGA GAATAAAGCC AAGAGCCACC TCCACATGGA AGCCCCCTTT GAGACAAAGG 
     2041 TCTCTACCCA CCTGAGGACT GTCATCCCCA TTGTCAATCA CTCCTCCAAG ATGACAATTC 
     2101 AGAAGGCCAT CAAGCAAAGC TTCTCCTTAG GATACATCCA CTTGGCCTTG TGTGCTGATG 
     2161 CCTGTGCAGG GAACCCTTTC CGGGACCACC TGAAGAAGCT GAATTTGAAA GTGGAGATCC 
     2221 AACTTCTTGA CCTCTGGCAG GACTTGCAGC ATTTCCTCAG TGTCCTTCTG AATAACAAAA 
     2281 AGAATGGGAA TGCAATCTTT CGTCACTTGC TGGGTGACAG AATCTGCGAG CTCTACCTGA 
     2341 ATGAGCAGAT TGGTCCGTGC TTACCACTCA AATCCCAAAC CATTCAGGGC CTGAAGGAAC 
     2401 TATTGCCCTC TGGGGATGTG ATCCCCTGGA TTCCCAAAGC CCAGAAGGAG ATTTGCAAGA 
     2461 TGCTCAGTCC CTGGTATGAT GAGTTTCTAG ATGAAGAGGA CTACTGGTTT CTCCTTTTTA 
     2521 CGACACAGAA CAGGTTCATC AGCTCCAGAC AGCATAAAAG AGAATTTATA GGCAAAGAAG 
     2581 AGAACATTCT TCTTTATAAG AGGATTCAGC AGTCTCTAGA GTTAAGCCAG GCTTTGGCTG 
     2641 ACATGAAGGA AATGGACTAT AGGCAGTGGC GAAAGATAGC TACTGAGGAC CTGAAGCAAG 
     2701 GAGGCTCTCT CCAGGTAGAG CTGACATCTC CAGTGTTTCT AACAGACATA ACTAAAATGT 
     2761 CCTTTGAGGA GCTTTGCTAC AAGAACCCAA AGATGGCCAT ACAGAAGATC AGTGATGACT 
     2821 ACAAAATATA CTGTGAGAAA GCACCTAAAA TAGATTTTAA AATGGAAATT ATCAAGGAAA 
     2881 CAAAGACAGT TTCACGCTCA AATAGGAAAA TGTCCTTGCT CAAAAGAACT CTTGTAAGGA 
     2941 AGCCATCAAT GAGACCCAGA AACTTGACAG AAGTCCTCTT AAATACACAG CACTTGGAGT 
     3001 TCTTCAGGGA GTTCCTCAAG GAACGGAAGG CTAAAATCCC ATTGCAATTT CTCACAGCTG 
     3061 TACAGAAGAT CAGTATAGAG ACCAATGAAA AGATTTGCAA GTCTCTCATA GAAAATGTAA 
     3121 TCAAGACTTT CTTCCAAGGC CAACTCTCTC CTGAAGAAAT GCTGCAGTGT GATGCCCCTA 
     3181 TTATCAAAGA AATCGCTTCC ATGCGTCATG TCACCACAAG CACACTGTTA ACGCTCCAGG 
     3241 GACATGTTAT GAAATCTATA GAAGAAAAGT GGTTTAAAGA TTATCAAGAC CTGTTCCCAC 
     3301 CTCACCATCA GGAGGTGGAA GTGCAAAGTG AAGTACAAAT TTCGTCTAGG AAGCCCTCAA 
     3361 AGATAGTGTC AACTTACCTA CAGGAATCCC AGAAGAAAGG CTGGATGAGA ATGATCAGCT 
     3421 TTATCAGGAG TTTTTGCAAG TACCGCAGAT TTATGTTGAA TCCTAGTAAG CGCCAGGAAT 
     3481 TTGAAGACTA TCTTCACCAG GAAATGCAAA ATAGCAAGGA AAATTTCACC ACAGCACACA 
     3541 ACACATCGGG ACGTTCAGCT CCACCCTCCA CAAATGTCCG GAGTGCAGAC CAAGAGAATG 
     3601 GAGAAATAAC CCTTGTAAAG CGTCGTATAT TTGGCCACAG GATTATCACT GTCAACTTTG 
     3661 CGATCAATGA TCTATATTTC TTTTCTGAAA TGGAGAAATT TAATGATCTG GTCAGTTCAG 
     3721 CCCACATGCT GCAGGTCAAC CGGGCATATA ATGAGAATGA TGTGATCCTA ATGCGGTCCA 
     3781 AAATGAACAT TATCCAAAAA CTCTTCCTGA ATTCTGACAT CCCTCCAAAG CTGAGGGTGA 
     3841 ATGTCCCTGA GTTCCAGAAG GATGCCATCC TTGCTGCCAT CACAGAGGGC TACCTAGATC 
     3901 GGAGCGTCTT CCATGGGGCT ATCATGTCTG TCTTCCCCGT TGTTATGTAC TTCTGGAAAA 
     3961 GGTTTTGTTT CTGGAAGGCA ACCCGCTCTT ACTTACAGTA TAGGGGGAAG AAGTTCAAGG 
     4021 ACAGAAAAAG CCCTCCTAAA TCTACGGACA AGTATCCTTT CTCGAGTGGA GGAGACAATG 
     4081 CCATCTTAAG GTTCACCTTG CTCAGAGGTA TTGAGTGGTT GCAGCCTCAA CGGGAAGCAA 
     4141 TAAGTTCAGT TCAAAATTCT TCATCAAGCA AACTTACTCA GCCAAGACTC GTGGTATCTG 
     4201 CCATGCAGCT GCATCCCGTC CAGGGACAAA AATTATCCTA CATCAAAAAA GAGAAGACGC 
     4261 GTACGCGGCC GCTCGAGCAG AAACTCATCT CAGAAGAGGA TCTGGCAGCA AATGATATCC 
     4321 TGGATTACAA GGATGACGAC GATAAGGTTT AAACGGCCGG CCGCGGTCAT AGCTGTTTCC 
     4381 TGAACAGATC CCGGGTGGCA TCCCTGTGAC CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA 
     4441 GTTGCCACTC CAGTGCCCAC CAGCCTTGTC CTAATAAAAT TAAGTTGCAT CATTTTGTCT 
     4501 GACTAGGTGT CCTTCTATAA TATTATGGGG TGGAGGGGGG TGGTATGGAG CAAGGGGCAA 
     4561 GTTGGGAAGA CAACCTGTAG GGCCTGCGGG GTCTATTGGG AACCAAGCTG GAGTGCAGTG 
     4621 GCACAATCTT GGCTCACTGC AATCTCCGCC TCCTGGGTTC AAGCGATTCT CCTGCCTCAG 
     4681 CCTCCCGAGT TGTTGGGATT CCAGGCATGC ATGACCAGGC TCAGCTAATT TTTGTTTTTT 
     4741 TGGTAGAGAC GGGGTTTCAC CATATTGGCC AGGCTGGTCT CCAACTCCTA ATCTCAGGTG 
     4801 ATCTACCCAC CTTGGCCTCC CAAATTGCTG GGATTACAGG CGTGAACCAC TGCTCCCTTC 
     4861 CCTGTCCTTC TGATTTTAAA ATAACTATAC CAGCAGGAGG ACGTCCAGAC ACAGCATAGG 
     4921 CTACCTGGCC ATGCCCAACC GGTGGGACAT TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA 
     4981 TGCGTTGGGT CCACTCAGTA GATGCCTGTT GAATTGGGTA CGCGGCCAGC GGCGAGCGGT 
     5041 ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA 
     5101 GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC 
     5161 GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG 
     5221 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT 
     5281 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG 
     5341 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG 
     5401 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG 
     5461 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC 
     5521 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG 
     5581 GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT 
     5641 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG 
     5701 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC 
     5761 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT 
     5821 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT 
     5881 TAAATCAATC TAAAGTATAT ATGAGTAACC TGAGGCTATG GCAGGGCCTG CCGCCCCGAC 
     5941 GTTGGCTGCG AGCCCTGGGC CTTCACCCGA ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA 
     6001 AACGCGGGCG TATTGGCCCC AATGGGGTCT CGGTGGGGTA TCGACAGAGT GCCAGCCCTG 
     6061 GGACCGAACC CCGCGTTTAT GAACAAACGA CCCAACACCG TGCGTTTTAT TCTGTCTTTT 
     6121 TATTGCCGTC ATAGCGCGGG TTCCTTCCGG TATTGTCTCC TTCCGTGTTT CAGTTAGCCT 
     6181 CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT GAGATCCCCG CGCTGGAGGA TCATCCAGCC 
     6241 GGCGTCCCGG AAAACGATTC CGAAGCCCAA CCTTTCATAG AAGGCGGCGG TGGAATCGAA 
     6301 ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG GTCGGTCATT TCGAACCCCA GAGTCCCGCT 
     6361 CAGAAGAACT CGTCAAGAAG GCGATAGAAG GCGATGCGCT GCGAATCGGG AGCGGCGATA 
     6421 CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT TCGCCGCCAA GCTCTTCAGC AATATCACGG 
     6481 GTAGCCAACG CTATGTCCTG ATAGCGATCC GCCACACCCA GCCGGCCACA GTCGATGAAT 
     6541 CCAGAAAAGC GGCCATTTTC CACCATGATA TTCGGCAAGC AGGCATCGCC ATGGGTCACG 
     6601 ACGAGATCCT CGCCGTCGGG CATGCTCGCC TTGAGCCTGG CGAACAGTTC GGCTGGCGCG 
     6661 AGCCCCTGAT GCTCTTCGTC CAGATCATCC TGATCGACAA GACCGGCTTC CATCCGAGTA 
     6721 CGTGCTCGCT CGATGCGATG TTTCGCTTGG TGGTCGAATG GGCAGGTAGC CGGATCAAGC 
     6781 GTATGCAGCC GCCGCATTGC ATCAGCCATG ATGGATACTT TCTCGGCAGG AGCAAGGTGA 
     6841 GATGACAGGA GATCCTGCCC CGGCACTTCG CCCAATAGCA GCCAGTCCCT TCCCGCTTCA 
     6901 GTGACAACGT CGAGCACAGC TGCGCAAGGA ACGCCCGTCG TGGCCAGCCA CGATAGCCGC 
     6961 GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA CCGGACAGGT CGGTCTTGAC AAAAAGAACC 
     7021 GGGCGCCCCT GCGCTGACAG CCGGAACACG GCGGCATCAG AGCAGCCGAT TGTCTGTTGT 
     7081 GCCCAGTCAT AGCCGAATAG CCTCTCCACC CAAGCGGCCG GAGAACCTGC GTGCAATCCA 
     7141 TCTTGTTCAA TCATGCGAAA CGATCCTCAT CCTGTCTCTT GATCGATCTT TGCAAAAGCC 
     7201 TAGGCCTCCA AAAAAGCCTC CTCACTACTT CTGGAATAGC TCAGAGGCCG AGGCGGCCTC 
     7261 GGCCTCTGCA TAAATAAAAA AAATTAGTCA GCCATGGGGC GGAGAATGGG CGGAACTGGG 
     7321 CGGAGTTAGG GGCGGGATGG GCGGAGTTAG GGGCGGGACT ATGGTTGCTG ACTAATTGAG 
     7381 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCTGGTTGC 
     7441 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     7501 ACACCCTAAC TGACACACAT TCCACAGCTG GTTCTTTCCG CCTCAGGACT CTTCCTTTTT 
     7561 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT 
     7621 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC 
     7681 GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT 
     7741 ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG 
     7801 TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT 
     7861 GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA 
     7921 TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA 
     7981 CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA 
     8041 GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC 
     8101 GCGAATTTT
//