LOCUS       RC226670                7668 bp    DNA     circular  SYN 
DEFINITION  ANO6 (Myc-DDK-tagged)-Human anoctamin 6 (ANO6), transcript variant 4
ACCESSION   NM_001142680
VERSION     NM_001142680.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3825..3916 
                     /label="Myc-DDK"  
     misc_feature    3946..4531 
                     /label="PolyA signal"  
     misc_feature    4003..4022 
                     /label="Restriction Enzyme"  
     misc_feature    4807..5479 
                     /label="ColE1"  
     misc_feature    5909..6703 
                     /label="Kan/Neo"  
     misc_feature    6738..7040 
                     /label="SV40 ori"  
     misc_feature    7126..7603 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3807..3813 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3807 
                     /label="ANO6"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAAAAAGATG AGCAGGAATG TTTTGCTACA AATGGAGGAG GAGGAGGACG 
     1081 ACGACGATGG GGATATCGTG TTGGAAAACC TTGGACAGAC AATTGTCCCC GATTTGGGAT 
     1141 CACTGGAAAG TCAGCATGAT TTTCGAACCC CGGAGTTTGA AGAATTTAAT GGAAAACCTG 
     1201 ACTCCCTCTT TTTTAATGAT GGCCAGCGAA GAATTGACTT TGTTCTAGTA TATGAGGATG 
     1261 AAAGCAGAAA AGAGACCAAT AAAAAGGGTA CAAATGAAAA ACAAAGGAGG AAAAGACAAG 
     1321 CATACGAATC TAACCTTATC TGTCATGGCC TGCAGTTAGA AGCAACAAGA TCAGTATTGG 
     1381 ATGACAAGCT TGTATTTGTA AAAGTACACG CACCATGGGA GGTGTTATGT ACGTATGCTG 
     1441 AGATAATGCA CATCAAATTG CCTCTGAAAC CCAATGATCT GAAAAACCGG TCCTCAGCCT 
     1501 TTGGTACACT CAACTGGTTT ACCAAAGTCC TCAGTGTAGA CGAAAGCATC ATCAAGCCAG 
     1561 AGCAAGAGTT TTTCACTGCC CCATTTGAGA AGAACCGGAT GAATGATTTT TACATAGTTG 
     1621 ATAGAGATGC TTTCTTCAAT CCAGCCACCA GAAGCCGCAT TGTTTACTTC ATCCTCTCTC 
     1681 GGGTCAAGTA TCAAGTGATA AACAATGTTA GCAAGTTTGG GATCAACAGA CTTGTAAACT 
     1741 CTGGGATCTA CAAGGCAGCT TTCCCACTCC ATGATTGCAA ATTCCGCCGT CAGTCAGAGG 
     1801 ATCCCAGCTG CCCTAATGAA CGGTACCTTC TGTACAGAGA ATGGGCTCAT CCTCGAAGCA 
     1861 TATACAAAAA GCAGCCCTTG GATCTTATCA GGAAATACTA TGGAGAGAAG ATTGGAATCT 
     1921 ACTTTGCTTG GCTGGGCTAT TACACTCAGA TGCTTCTCCT GGCCGCAGTT GTAGGAGTGG 
     1981 CTTGCTTTCT CTATGGATAT CTTAATCAAG ATAACTGTAC ATGGAGCAAA GAAGTTTGTC 
     2041 ATCCTGATAT TGGTGGCAAG ATCATAATGT GTCCTCAGTG TGATAGGCTT TGTCCATTCT 
     2101 GGAAACTCAA TATTACTTGC GAGTCCTCAA AGAAATTGTG CATCTTCGAC AGTTTTGGAA 
     2161 CCCTGGTCTT TGCAGTATTT ATGGGAGTAT GGGTTACCTT GTTTTTGGAG TTTTGGAAGC 
     2221 GACGCCAGGC AGAACTTGAG TATGAATGGG ATACTGTTGA GTTACAGCAG GAAGAACAAG 
     2281 CCCGACCAGA ATACGAAGCA CGATGTACTC ACGTAGTGAT AAATGAGATT ACTCAGGAAG 
     2341 AAGAACGCAT TCCCTTTACT GCCTGGGGAA AATGTATACG GATAACCCTC TGTGCCAGTG 
     2401 CTGTCTTTTT CTGGATCCTA TTGATCATCG CTTCAGTTAT TGGGATCATT GTCTATAGGC 
     2461 TCTCGGTGTT CATTGTATTT TCTGCAAAAC TTCCCAAGAA CATTAATGGA ACAGACCCAA 
     2521 TCCAGAAATA CCTGACTCCA CAGACAGCCA CGTCCATCAC GGCCTCCATC ATCAGCTTTA 
     2581 TAATTATCAT GATTCTGAAC ACCATATATG AAAAAGTGGC AATTATGATT ACTAACTTCG 
     2641 AACTCCCAAG GACCCAGACT GATTATGAGA ACAGCCTCAC CATGAAGATG TTCTTATTCC 
     2701 AGTTTGTCAA CTACTACTCT TCATGCTTCT ACATAGCATT CTTTAAGGGC AAATTTGTAG 
     2761 GCTATCCAGG AGACCCAGTT TATTGGTTGG GAAAATACAG AAATGAAGAG TGTGACCCAG 
     2821 GTGGCTGTCT TCTTGAACTG ACAACTCAGC TGACAATAAT CATGGGAGGA AAAGCAATCT 
     2881 GGAATAACAT ACAAGAAGTA TTATTGCCCT GGATCATGAA TCTAATTGGG CGATTTCACA 
     2941 GAGTTTCTGG ATCAGAAAAG ATAACCCCAC GATGGGAACA GGACTACCAT CTGCAGCCTA 
     3001 TGGGCAAACT GGGATTATTT TATGAATATC TTGAAATGAT TATTCAGTTT GGGTTCGTCA 
     3061 CCTTATTTGT GGCCTCTTTT CCACTGGCCC CTCTGTTGGC TCTCGTGAAC AATATATTGG 
     3121 AAATAAGAGT GGACGCATGG AAACTGACCA CCCAGTTTAG ACGCCTGGTA CCAGAGAAAG 
     3181 CCCAAGACAT TGGAGCATGG CAGCCCATCA TGCAAGGAAT AGCAATTCTG GCTGTGGTGA 
     3241 CCAATGCCAT GATCATAGCT TTCACGTCGG ACATGATCCC CCGCCTAGTG TACTACTGGT 
     3301 CCTTCTCCGT CCCTCCCTAC GGGGACCACA CTTCCTACAC CATGGAAGGG TACATCAACA 
     3361 ACACTCTCTC CATCTTCAAA GTCGCAGACT TCAAAAACAA AAGCAAGGGA AACCCGTACT 
     3421 CTGACCTGGG TAACCATACC ACATGCAGGT ATCGTGATTT CCGATACCCA CCTGGACACC 
     3481 CCCAGGAGTA TAAACACAAC ATCTACTATT GGCATGTGAT TGCAGCCAAG CTGGCTTTTA 
     3541 TCATTGTCAT GGAGTATCTC GCTTTGTTGC CCAGGCTGGG ACACAGTGGC ATGATCTTGG 
     3601 CTCACTGCAA CCTCCGCCTC CCAGTTGACT GCTGTATGTG TTATAGGTTT GTCGATGAAA 
     3661 TTAGACTTTT GGAACAACTA ACTTCTGACT TTATTGACTC CCTCTATTAT ATATTTTCTA 
     3721 TTTCAATAAT ATCTATTTTC TTTTCTGTTA CTTTCTTTTT CCTTCTACTT TCTTTGGGGC 
     3781 CAACTCCGTG TTTTTCTGTA TCGAATTTCT TAAGTACGCG TACGCGGCCG CTCGAGCAGA 
     3841 AACTCATCTC AGAAGAGGAT CTGGCAGCAA ATGATATCCT GGATTACAAG GATGACGACG 
     3901 ATAAGGTTTA AACGGCCGGC CGCGGTCATA GCTGTTTCCT GAACAGATCC CGGGTGGCAT 
     3961 CCCTGTGACC CCTCCCCAGT GCCTCTCCTG GCCCTGGAAG TTGCCACTCC AGTGCCCACC 
     4021 AGCCTTGTCC TAATAAAATT AAGTTGCATC ATTTTGTCTG ACTAGGTGTC CTTCTATAAT 
     4081 ATTATGGGGT GGAGGGGGGT GGTATGGAGC AAGGGGCAAG TTGGGAAGAC AACCTGTAGG 
     4141 GCCTGCGGGG TCTATTGGGA ACCAAGCTGG AGTGCAGTGG CACAATCTTG GCTCACTGCA 
     4201 ATCTCCGCCT CCTGGGTTCA AGCGATTCTC CTGCCTCAGC CTCCCGAGTT GTTGGGATTC 
     4261 CAGGCATGCA TGACCAGGCT CAGCTAATTT TTGTTTTTTT GGTAGAGACG GGGTTTCACC 
     4321 ATATTGGCCA GGCTGGTCTC CAACTCCTAA TCTCAGGTGA TCTACCCACC TTGGCCTCCC 
     4381 AAATTGCTGG GATTACAGGC GTGAACCACT GCTCCCTTCC CTGTCCTTCT GATTTTAAAA 
     4441 TAACTATACC AGCAGGAGGA CGTCCAGACA CAGCATAGGC TACCTGGCCA TGCCCAACCG 
     4501 GTGGGACATT TGAGTTGCTT GCTTGGCACT GTCCTCTCAT GCGTTGGGTC CACTCAGTAG 
     4561 ATGCCTGTTG AATTGGGTAC GCGGCCAGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT 
     4621 AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA 
     4681 GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC 
     4741 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT 
     4801 ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT 
     4861 GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG 
     4921 CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA 
     4981 CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA 
     5041 CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC 
     5101 GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG 
     5161 AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG 
     5221 TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA 
     5281 GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC 
     5341 TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG 
     5401 GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA 
     5461 TGAGTAACCT GAGGCTATGG CAGGGCCTGC CGCCCCGACG TTGGCTGCGA GCCCTGGGCC 
     5521 TTCACCCGAA CTTGGGGGGT GGGGTGGGGA AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA 
     5581 ATGGGGTCTC GGTGGGGTAT CGACAGAGTG CCAGCCCTGG GACCGAACCC CGCGTTTATG 
     5641 AACAAACGAC CCAACACCGT GCGTTTTATT CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT 
     5701 TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA 
     5761 CTCCAGCATG AGATCCCCGC GCTGGAGGAT CATCCAGCCG GCGTCCCGGA AAACGATTCC 
     5821 GAAGCCCAAC CTTTCATAGA AGGCGGCGGT GGAATCGAAA TCTCGTGATG GCAGGTTGGG 
     5881 CGTCGCTTGG TCGGTCATTT CGAACCCCAG AGTCCCGCTC AGAAGAACTC GTCAAGAAGG 
     5941 CGATAGAAGG CGATGCGCTG CGAATCGGGA GCGGCGATAC CGTAAAGCAC GAGGAAGCGG 
     6001 TCAGCCCATT CGCCGCCAAG CTCTTCAGCA ATATCACGGG TAGCCAACGC TATGTCCTGA 
     6061 TAGCGATCCG CCACACCCAG CCGGCCACAG TCGATGAATC CAGAAAAGCG GCCATTTTCC 
     6121 ACCATGATAT TCGGCAAGCA GGCATCGCCA TGGGTCACGA CGAGATCCTC GCCGTCGGGC 
     6181 ATGCTCGCCT TGAGCCTGGC GAACAGTTCG GCTGGCGCGA GCCCCTGATG CTCTTCGTCC 
     6241 AGATCATCCT GATCGACAAG ACCGGCTTCC ATCCGAGTAC GTGCTCGCTC GATGCGATGT 
     6301 TTCGCTTGGT GGTCGAATGG GCAGGTAGCC GGATCAAGCG TATGCAGCCG CCGCATTGCA 
     6361 TCAGCCATGA TGGATACTTT CTCGGCAGGA GCAAGGTGAG ATGACAGGAG ATCCTGCCCC 
     6421 GGCACTTCGC CCAATAGCAG CCAGTCCCTT CCCGCTTCAG TGACAACGTC GAGCACAGCT 
     6481 GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC GATAGCCGCG CTGCCTCGTC TTGCAGTTCA 
     6541 TTCAGGGCAC CGGACAGGTC GGTCTTGACA AAAAGAACCG GGCGCCCCTG CGCTGACAGC 
     6601 CGGAACACGG CGGCATCAGA GCAGCCGATT GTCTGTTGTG CCCAGTCATA GCCGAATAGC 
     6661 CTCTCCACCC AAGCGGCCGG AGAACCTGCG TGCAATCCAT CTTGTTCAAT CATGCGAAAC 
     6721 GATCCTCATC CTGTCTCTTG ATCGATCTTT GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC 
     6781 TCACTACTTC TGGAATAGCT CAGAGGCCGA GGCGGCCTCG GCCTCTGCAT AAATAAAAAA 
     6841 AATTAGTCAG CCATGGGGCG GAGAATGGGC GGAACTGGGC GGAGTTAGGG GCGGGATGGG 
     6901 CGGAGTTAGG GGCGGGACTA TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     6961 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCTGGTTGCT GACTAATTGA GATGCATGCT 
     7021 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCCTAACT GACACACATT 
     7081 CCACAGCTGG TTCTTTCCGC CTCAGGACTC TTCCTTTTTC AATATTATTG AAGCATTTAT 
     7141 CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA 
     7201 GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG CGCCCTGTAG CGGCGCATTA 
     7261 AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG 
     7321 CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA 
     7381 GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC 
     7441 AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT 
     7501 CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA 
     7561 ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC 
     7621 TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTT
//