LOCUS       RC226620                8754 bp    DNA     circular  SYN 
DEFINITION  SETDB1 (Myc-DDK-tagged)-Human SET domain, bifurcated 1 (SETDB1), transcript variant 1
ACCESSION   NM_001145415
VERSION     NM_001145415.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4911..5002 
                     /label="Myc-DDK"  
     misc_feature    5032..5617 
                     /label="PolyA signal"  
     misc_feature    5089..5108 
                     /label="Restriction Enzyme"  
     misc_feature    5893..6565 
                     /label="ColE1"  
     misc_feature    6995..7789 
                     /label="Kan/Neo"  
     misc_feature    7824..8126 
                     /label="SV40 ori"  
     misc_feature    8212..8689 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4893..4899 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4893 
                     /label="SETDB1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCTTCCCTT CCTGGGTGCA TTGGTTTGGA TGCAGCAACA GCTACAGTGG 
     1081 AGTCTGAAGA GATTGCAGAG CTGCAACAGG CAGTGGTTGA GGAACTGGGT ATCTCTATGG 
     1141 AGGAACTTCG GCATTTCATC GATGAGGAAC TGGAGAAGAT GGATTGTGTA CAGCAACGCA 
     1201 AGAAGCAGCT AGCAGAGTTA GAGACATGGG TAATACAGAA AGAATCTGAG GTGGCTCACG 
     1261 TTGACCAACT CTTTGATGAT GCATCCAGGG CAGTGACTAA TTGTGAGTCT TTGGTGAAGG 
     1321 ACTTCTACTC CAAGCTGGGA CTACAATACC GGGACAGTAG CTCTGAGGAC GAATCTTCCC 
     1381 GGCCTACAGA AATAATTGAG ATTCCTGATG AAGATGATGA TGTCCTCAGT ATTGATTCAG 
     1441 GTGATGCTGG GAGCAGAACT CCAAAAGACC AGAAGCTCCG TGAAGCTATG GCTGCCTTAA 
     1501 GAAAGTCAGC TCAAGATGTT CAGAAGTTCA TGGATGCTGT CAACAAGAAG AGCAGTTCCC 
     1561 AGGATCTGCA TAAAGGAACC TTGAGTCAGA TGTCTGGAGA ACTAAGCAAA GATGGTGACC 
     1621 TGATAGTCAG CATGCGAATT CTGGGCAAGA AGAGAACTAA GACTTGGCAC AAAGGCACCC 
     1681 TTATTGCCAT CCAGACAGTT GGGCCAGGGA AGAAATACAA GGTGAAATTT GACAACAAAG 
     1741 GAAAGAGTCT ACTGTCGGGG AACCATATTG CCTATGATTA CCACCCTCCT GCTGACAAGC 
     1801 TGTATGTGGG CAGTCGGGTG GTCGCCAAAT ACAAAGATGG GAATCAGGTC TGGCTCTATG 
     1861 CTGGCATTGT AGCTGAGACA CCAAACGTCA AAAACAAGCT CAGGTTTCTC ATTTTCTTTG 
     1921 ATGATGGCTA TGCTTCCTAT GTCACACAGT CGGAACTGTA TCCCATTTGC CGGCCACTGA 
     1981 AAAAGACTTG GGAGGACATA GAAGACATCT CCTGCCGTGA CTTCATAGAG GAGTATGTCA 
     2041 CTGCCTACCC CAACCGCCCC ATGGTACTGC TCAAGAGTGG CCAGCTTATC AAGACTGAGT 
     2101 GGGAAGGCAC GTGGTGGAAG TCCCGAGTTG AGGAGGTGGA TGGCAGCCTA GTCAGGATCC 
     2161 TCTTCCTGGA TGACAAAAGA TGTGAGTGGA TCTATCGAGG CTCTACACGG CTGGAGCCCA 
     2221 TGTTCAGCAT GAAAACATCC TCAGCCTCTG CACTGGAGAA GAAGCAAGGA CAGCTCAGGA 
     2281 CACGTCCAAA TATGGGTGCT GTGAGGAGCA AAGGCCCTGT TGTCCAGTAC ACACAGGATC 
     2341 TGACCGGTAC TGGAACCCAG TTCAAGCCAG TGGAACCCCC ACAGCCTACA GCTCCACCTG 
     2401 CCCCACCTTT CCCACCTGCT CCACCTCTAT CCCCCCAAGC AGGTGACAGT GACTTGGAAA 
     2461 GCCAGCTTGC CCAGTCACGG AAGCAGGTAG CCAAAAAGAG CACGTCCTTT CGACCAGGAT 
     2521 CTGTGGGCTC TGGTCATTCC TCCCCTACAT CTCCTGCACT CAGTGAAAAT GTCTCTGGTG 
     2581 GGAAACCTGG GATCAACCAG ACATATAGAT CACCTTTAGG CTCCACAGCC TCTGCCCCAG 
     2641 CACCCTCAGC ACTCCCGGCC CCTCCAGCAC CCCCAGTCTT CCATGGCATG CTGGAGCGGG 
     2701 CCCCAGCAGA GCCCTCCTAC CGTGCTCCCA TGGAGAAGCT TTTCTACTTA CCTCATGTCT 
     2761 GCAGCTATAC CTGCCTGTCT CGAGTCAGAC CTATGAGGAA TGAGCAGTAC CGGGGCAAGA 
     2821 ACCCTCTGCT GGTCCCGTTA CTATATGACT TCCGGCGGAT GACAGCCCGG CGTCGAGTTA 
     2881 ACCGCAAGAT GGGCTTTCAT GTTATCTATA AGACACCTTG TGGTCTCTGC CTTCGGACAA 
     2941 TGCAGGAGAT AGAACGCTAC CTTTTCGAGA CTGGCTGTGA CTTCCTCTTC CTGGAGATGT 
     3001 TCTGTTTGGA TCCATATGTT CTTGTGGACC GAAAGTTTCA GCCCTATAAG CCTTTTTACT 
     3061 ATATTTTGGA CATCACTTAT GGGAAGGAAG ATGTTCCCCT ATCCTGTGTC AATGAGATTG 
     3121 ACACAACCCC TCCACCCCAG GTGGCCTACA GCAAGGAACG TATCCCGGGC AAGGGTGTTT 
     3181 TCATTAACAC AGGCCCTGAA TTTCTGGTTG GCTGTGACTG CAAGGATGGG TGTCGGGACA 
     3241 AGTCCAAGTG TGCCTGCCAT CAACTAACTA TCCAGGCTAC AGCCTGTACC CCAGGAGGCC 
     3301 AAATCAACCC TAACTCTGGC TACCAGTACA AGAGACTAGA AGAGTGTCTA CCCACAGGGG 
     3361 TATATGAGTG TAACAAACGC TGCAAATGTG ACCCAAACAT GTGCACAAAC CGGTTGGTGC 
     3421 AACATGGACT ACAAGTTCGG CTACAGCTAT TCAAGACACA GAACAAGGGC TGGGGTATCC 
     3481 GCTGCTTGGA TGACATTGCC AAAGGCTCTT TTGTTTGTAT TTATGCAGGC AAAATCCTGA 
     3541 CAGATGACTT TGCAGACAAG GAGGGTCTGG AAATGGGTGA TGAGTACTTT GCAAATCTGG 
     3601 ACCATATCGA GAGCGTGGAG AACTTCAAAG AAGGATATGA GAGTGATGCC CCCTGTTCCT 
     3661 CTGACAGCAG TGGTGTAGAC TTGAAGGACC AGGAAGATGG CAACAGCGGT ACAGAGGACC 
     3721 CTGAAGAGTC CAATGATGAT AGCTCAGATG ATAACTTCTG TAAGGATGAG GACTTCAGCA 
     3781 CCAGTTCAGT GTGGCGGAGC TATGCTACCC GGAGGCAGAC CCGGGGCCAG AAAGAGAACG 
     3841 GACTCTCTGA GACAACTTCC AAGGACTCCC ACCCCCCAGA TCTTGGACCC CCACATATTC 
     3901 CTGTTCCTCC CTCAATCCCT GTAGGTGGCT GCAATCCACC TTCCTCCGAA GAGACACCCA 
     3961 AGAACAAGGT GGCCTCATGG TTGAGCTGCA ATAGTGTCAG TGAAGGTGGT TTTGCTGACT 
     4021 CTGATAGCCA TTCATCCTTC AAGACTAATG AAGGTGGGGA GGGCCGGGCT GGGGGAAGCC 
     4081 GAATGGAGGC TGAGAAGGCC TCCACCTCAG GACTAGGCAT CAAGGATGAG GGAGACATCA 
     4141 AACAGGCCAA GAAAGAGGAC ACTGACGACC GAAACAAGAT GTCAGTAGTT ACTGAAAGCT 
     4201 CTCGAAATTA CGGTTACAAT CCTTCTCCTG TGAAGCCTGA AGGACTTCGC CGCCCACCTA 
     4261 GTAAGACTAG TATGCATCAA AGCCGAAGAC TCATGGCTTC TGCTCAGTCC AACCCTGATG 
     4321 ATGTCCTGAC ACTGTCCAGC AGCACAGAAA GTGAGGGGGA AAGTGGGACC AGCCGAAAGC 
     4381 CCACTGCTGG TCAGACTTCG GCTACAGCGG TTGACAGTGA TGATATCCAG ACCATATCCT 
     4441 CTGGCTCTGA AGGGGATGAC TTTGAGGACA AGAAGAACAT GACTGGTCCA ATGAAGCGTC 
     4501 AAGTGGCAGT AAAATCAACC CGAGGCTTTG CTCTTAAATC AACCCATGGG ATTGCAATTA 
     4561 AATCAACCAA CATGGCCTCT GTGGACAAGG GGGAGAGCGC ACCTGTTCGT AAGAACACAC 
     4621 GCCAATTCTA TGATGGCGAG GAGTCTTGCT ACATCATTGA TGCCAAGCTT GAAGGCAACC 
     4681 TGGGCCGCTA CCTCAACCAC AGTTGCAGCC CCAACCTGTT TGTCCAGAAT GTCTTCGTGG 
     4741 ATACCCATGA TCTTCGCTTC CCCTGGGTGG CCTTCTTTGC CAGCAAAAGA ATCCGGGCTG 
     4801 GGACAGAACT TACTTGGGAC TACAACTACG AGGTGGGCAG TGTGGAAGGC AAGGAGCTAC 
     4861 TCTGTTGCTG TGGGGCCATT GAATGCAGAG GACGTCTTCT TACGCGTACG CGGCCGCTCG 
     4921 AGCAGAAACT CATCTCAGAA GAGGATCTGG CAGCAAATGA TATCCTGGAT TACAAGGATG 
     4981 ACGACGATAA GGTTTAAACG GCCGGCCGCG GTCATAGCTG TTTCCTGAAC AGATCCCGGG 
     5041 TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG 
     5101 CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC 
     5161 TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC 
     5221 TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC 
     5281 ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG 
     5341 GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT 
     5401 TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG 
     5461 CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT 
     5521 TTAAAATAAC TATACCAGCA GGAGGACGTC CAGACACAGC ATAGGCTACC TGGCCATGCC 
     5581 CAACCGGTGG GACATTTGAG TTGCTTGCTT GGCACTGTCC TCTCATGCGT TGGGTCCACT 
     5641 CAGTAGATGC CTGTTGAATT GGGTACGCGG CCAGCGGCGA GCGGTATCAG CTCACTCAAA 
     5701 GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA 
     5761 AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT 
     5821 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 
     5881 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 
     5941 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 
     6001 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 
     6061 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 
     6121 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 
     6181 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 
     6241 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 
     6301 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 
     6361 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC 
     6421 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC 
     6481 AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG 
     6541 TATATATGAG TAACCTGAGG CTATGGCAGG GCCTGCCGCC CCGACGTTGG CTGCGAGCCC 
     6601 TGGGCCTTCA CCCGAACTTG GGGGGTGGGG TGGGGAAAAG GAAGAAACGC GGGCGTATTG 
     6661 GCCCCAATGG GGTCTCGGTG GGGTATCGAC AGAGTGCCAG CCCTGGGACC GAACCCCGCG 
     6721 TTTATGAACA AACGACCCAA CACCGTGCGT TTTATTCTGT CTTTTTATTG CCGTCATAGC 
     6781 GCGGGTTCCT TCCGGTATTG TCTCCTTCCG TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC 
     6841 GAAGAACTCC AGCATGAGAT CCCCGCGCTG GAGGATCATC CAGCCGGCGT CCCGGAAAAC 
     6901 GATTCCGAAG CCCAACCTTT CATAGAAGGC GGCGGTGGAA TCGAAATCTC GTGATGGCAG 
     6961 GTTGGGCGTC GCTTGGTCGG TCATTTCGAA CCCCAGAGTC CCGCTCAGAA GAACTCGTCA 
     7021 AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA TCGGGAGCGG CGATACCGTA AAGCACGAGG 
     7081 AAGCGGTCAG CCCATTCGCC GCCAAGCTCT TCAGCAATAT CACGGGTAGC CAACGCTATG 
     7141 TCCTGATAGC GATCCGCCAC ACCCAGCCGG CCACAGTCGA TGAATCCAGA AAAGCGGCCA 
     7201 TTTTCCACCA TGATATTCGG CAAGCAGGCA TCGCCATGGG TCACGACGAG ATCCTCGCCG 
     7261 TCGGGCATGC TCGCCTTGAG CCTGGCGAAC AGTTCGGCTG GCGCGAGCCC CTGATGCTCT 
     7321 TCGTCCAGAT CATCCTGATC GACAAGACCG GCTTCCATCC GAGTACGTGC TCGCTCGATG 
     7381 CGATGTTTCG CTTGGTGGTC GAATGGGCAG GTAGCCGGAT CAAGCGTATG CAGCCGCCGC 
     7441 ATTGCATCAG CCATGATGGA TACTTTCTCG GCAGGAGCAA GGTGAGATGA CAGGAGATCC 
     7501 TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG TCCCTTCCCG CTTCAGTGAC AACGTCGAGC 
     7561 ACAGCTGCGC AAGGAACGCC CGTCGTGGCC AGCCACGATA GCCGCGCTGC CTCGTCTTGC 
     7621 AGTTCATTCA GGGCACCGGA CAGGTCGGTC TTGACAAAAA GAACCGGGCG CCCCTGCGCT 
     7681 GACAGCCGGA ACACGGCGGC ATCAGAGCAG CCGATTGTCT GTTGTGCCCA GTCATAGCCG 
     7741 AATAGCCTCT CCACCCAAGC GGCCGGAGAA CCTGCGTGCA ATCCATCTTG TTCAATCATG 
     7801 CGAAACGATC CTCATCCTGT CTCTTGATCG ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA 
     7861 GCCTCCTCAC TACTTCTGGA ATAGCTCAGA GGCCGAGGCG GCCTCGGCCT CTGCATAAAT 
     7921 AAAAAAAATT AGTCAGCCAT GGGGCGGAGA ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG 
     7981 GATGGGCGGA GTTAGGGGCG GGACTATGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     8041 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCTG GTTGCTGACT AATTGAGATG 
     8101 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC CTAACTGACA 
     8161 CACATTCCAC AGCTGGTTCT TTCCGCCTCA GGACTCTTCC TTTTTCAATA TTATTGAAGC 
     8221 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA 
     8281 CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC 
     8341 GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC 
     8401 CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC 
     8461 CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC 
     8521 GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG 
     8581 GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT 
     8641 GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT 
     8701 TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
//