LOCUS       RC226407                9525 bp    DNA     circular  SYN 
DEFINITION  NFAT5 (Myc-DDK-tagged)-Human nuclear factor of activated T-cells 5, tonicity-responsive (NFAT5), transcript variant 6
ACCESSION   NM_001113178
VERSION     NM_001113178.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5682..5773 
                     /label="Myc-DDK"  
     misc_feature    5803..6388 
                     /label="PolyA signal"  
     misc_feature    5860..5879 
                     /label="Restriction Enzyme"  
     misc_feature    6664..7336 
                     /label="ColE1"  
     misc_feature    7766..8560 
                     /label="Kan/Neo"  
     misc_feature    8595..8897 
                     /label="SV40 ori"  
     misc_feature    8983..9460 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5664..5670 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5664 
                     /label="NFAT5"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCCTCGGAC TTCATCTCAT TGCTCAGCGC GGACCTAGAC CTGGAATCGC 
     1081 CCAAGTCCCT CTACTCGCGA GATTCTCTGA AGTTACACCC ATCACAGAAT TTTCATAGAG 
     1141 CTGGACTATT GGAAGAATCT GTCTATGATC TTCTCCCAAA GGAGTTACAG TTACCTCCAT 
     1201 CTAGAGAAAC ATCTGTAGCA TCAATGAGTC AGACAAGCGG TGGTGAGGCA GGCTCGCCTC 
     1261 CTCCAGCTGT TGTTGCTGCT GATGCTTCTT CAGCTCCCTC CTCTTCCTCC ATGGGCGGTG 
     1321 CTTGCAGCTC CTTTACCACC TCTTCCAGCC CTACCATTTA TTCTACCTCA GTCACCGACA 
     1381 GCAAGGCTAT GCAAGTGGAG AGCTGCTCCT CAGCCGTGGG GGTAAGTAAC AGAGGGGTAA 
     1441 GTGAAAAGCA GTTAACCAGT AACACAGTTC AGCAGCATCC ATCAACACCG AAGAGGCACA 
     1501 CAGTCTTGTA CATCTCACCA CCACCTGAGG ACTTGCTGGA TAACAGTCGG ATGTCCTGCC 
     1561 AGGATGAGGG GTGTGGATTG GAATCTGAGC AGAGCTGCAG TATGTGGATG GAGGATTCCC 
     1621 CCTCCAACTT CAGTAACATG AGCACCAGTT CCTACAATGA TAACACTGAG GTACCTCGTA 
     1681 AATCACGAAA ACGAAATCCA AAGCAGAGGC CGGGGGTCAA ACGACGAGAT TGTGAAGAAT 
     1741 CTAATATGGA TATATTTGAT GCCGACAGTG CCAAAGCACC TCACTATGTG CTTTCTCAGC 
     1801 TTACCACGGA CAACAAAGGC AACTCAAAAG CGGGAAATGG AACATTGGAA AACCAAAAAG 
     1861 GAACTGGAGT AAAGAAGAGC CCTATGTTGT GTGGACAATA TCCTGTTAAA AGTGAGGGAA 
     1921 AGGAGCTGAA GATAGTTGTA CAACCTGAGA CACAGCACCG AGCTCGGTAC CTGACTGAGG 
     1981 GCAGCCGTGG CTCAGTGAAA GATAGAACAC AGCAAGGCTT TCCTACAGTA AAGCTGGAAG 
     2041 GCCATAATGA ACCTGTAGTG TTGCAAGTGT TTGTGGGCAA CGACTCTGGA CGAGTGAAAC 
     2101 CACATGGATT TTATCAGGCC TGCAGAGTAA CTGGACGAAA TACAACTCCT TGCAAAGAAG 
     2161 TGGACATTGA AGGCACTACT GTTATAGAAG TCGGCCTTGA TCCTAGCAAC AACATGACAC 
     2221 TGGCGGTGGA CTGCGTAGGG ATATTGAAAT TGAGGAATGC TGATGTCGAA GCCAGAATAG 
     2281 GAATTGCTGG TTCCAAGAAG AAAAGCACTC GTGCCAGATT GGTTTTTCGA GTTAATATCA 
     2341 TGAGGAAAGA TGGCTCCACT TTGACACTGC AAACACCCTC TTCTCCAATT TTGTGTACTC 
     2401 AGCCAGCAGG AGTGCCAGAA ATCTTAAAGA AAAGCTTGCA TAGCTGTTCA GTGAAAGGAG 
     2461 AAGAAGAAGT GTTTTTAATC GGCAAGAACT TTCTGAAAGG AACTAAAGTT ATTTTCCAAG 
     2521 AAAATGTTTC TGATGAAAAC TCTTGGAAGT CAGAAGCTGA AATTGATATG GAACTATTTC 
     2581 ATCAGAATCA TCTTATTGTG AAGGTTCCTC CCTATCATGA CCAACATATA ACTTTGCCTG 
     2641 TGTCAGTGGG AATATATGTA GTGACAAATG CTGGAAGATC TCATGATGTT CAACCATTCA 
     2701 CTTACACTCC AGACCCAGCA GCTGGTGCTT TGAATGTAAA TGTGAAGAAG GAAATATCTA 
     2761 GTCCAGCAAG ACCTTGCTCT TTTGAAGAGG CCATGAAAGC AATGAAAACT ACTGGATGTA 
     2821 ATTTAGATAA GGTAAATATT ATCCCTAATG CCCTGATGAC TCCACTCATA CCAAGCAGTA 
     2881 TGATTAAGAG TGAAGATGTT ACTCCAATGG AAGTAACAGC AGAAAAAAGA TCTTCCACTA 
     2941 TTTTTAAGAC TACAAAGTCT GTTGGATCAA CTCAGCAAAC ATTAGAAAAC ATCTCAAACA 
     3001 TAGCAGGAAA TGGCTCTTTT TCATCACCAT CATCTTCCCA CCTACCTTCT GAAAATGAAA 
     3061 AACAGCAGCA GATTCAGCCC AAGGCATACA ACCCAGAGAC CCTGACAACT ATTCAAACCC 
     3121 AGGACATCTC ACAGCCTGGT ACTTTTCCAG CAGTTTCTGC TTCTAGTCAG CTGCCCAACA 
     3181 GCGATGCACT ATTGCAGCAG GCTACACAGT TTCAGACAAG AGAAACTCAG TCTAGAGAGA 
     3241 TATTACAGTC AGATGGTACA GTGGTTAATT TGTCACAACT GACTGAGGCA TCACAACAAC 
     3301 AGCAGCAGTC ACCACTACAA GAACAAGCAC AGACTTTACA GCAGCAGATT TCATCAAATA 
     3361 TTTTTCCATC ACCAAATAGT GTGAGTCAGC TTCAGAATAC TATTCAGCAG CTGCAAGCAG 
     3421 GGAGTTTCAC AGGCAGTACT GCTAGTGGCA GCAGTGGAAG TGTTGACTTG GTCCAACAAG 
     3481 TTTTAGAGGC ACAGCAGCAG TTATCTTCAG TTTTATTTTC TGCTCCAGAT GGTAATGAGA 
     3541 ATGTTCAAGA GCAGCTTAGT GCAGATATTT TTCAACAAGT CAGTCAAATT CAGAGTGGTG 
     3601 TAAGCCCTGG AATGTTTTCC TCAACAGAGC CAACAGTCCA TACCAGACCA GATAATTTAT 
     3661 TACCTGGAAG AGCTGAAAGT GTTCATCCAC AGTCTGAAAA CACGTTATCT AATCAACAGC 
     3721 AGCAGCAGCA GCAGCAACAG CAAGTGATGG AATCTTCAGC CGCAATGGTG ATGGAGATGC 
     3781 AACAGAGTAT CTGCCAGGCA GCTGCCCAGA TTCAGTCAGA GTTATTCCCT TCAACTGCTT 
     3841 CAGCAAATGG AAACCTTCAG CAATCGCCAG TTTACCAGCA GACTTCTCAC ATGATGAGTG 
     3901 CATTGTCTAC CAATGAGGAT ATGCAAATGC AGTGTGAATT GTTTTCTTCT CCTCCTGCAG 
     3961 TTTCTGGAAA TGAAACTTCT ACAACTACCA CACAGCAGGT TGCAACCCCT GGCACTACCA 
     4021 TGTTTCAGAC ATCAAGTTCA GGAGATGGAG AAGAAACTGG AACACAAGCA AAACAGATTC 
     4081 AGAACAGTGT CTTTCAGACC ATGGTCCAAA TGCAACATAG TGGGGACAAT CAACCTCAAG 
     4141 TTAACCTTTT TTCATCCACA AAAAGTATGA TGAGTGTTCA GAATAGTGGT ACCCAACAAC 
     4201 AAGGTAATGG TTTATTCCAG CAAGGGAATG AGATGATGTC ACTTCAATCT GGAAATTTTT 
     4261 TGCAGCAGTC TTCTCATTCA CAGGCCCAAC TTTTTCATCC TCAAAATCCT ATTGCCGATG 
     4321 CTCAGAACCT TTCCCAGGAA ACTCAAGGTT CTCTCTTTCA TAGTCCAAAT CCTATTGTCC 
     4381 ACAGTCAGAC TTCTACAACC TCCTCTGAAC AAATGCAGCC TCCAATGTTT CACTCTCAAA 
     4441 GTACCATTGC TGTGTTACAG GGCTCTTCAG TTCCTCAAGA CCAGCAGTCA ACCAACATAT 
     4501 TTCTTTCCCA GAGTCCCATG AATAATCTTC AGACTAACAC AGTAGCCCAA GAAGCATTTT 
     4561 TTGCAGCACC GAACTCAATT TCTCCACTTC AGTCAACATC AAACAGTGAA CAACAAGCTG 
     4621 CTTTCCAACA GCAAGCTCCA ATATCACACA TCCAGACTCC TATGCTTTCC CAAGAACAGG 
     4681 CACAACCCCC GCAGCAGGGT TTATTTCAGC CTCAGGTGGC CCTGGGCTCC CTTCCACCTA 
     4741 ATCCAATGCC TCAAAGCCAA CAAGGAACCA TGTTCCAGTC ACAGCACTCA ATAGTTGCCA 
     4801 TGCAGAGTAA CTCTCCATCC CAGGAACAGC AGCAGCAGCA GCAACAGCAG CAGCAACAGC 
     4861 AGCAGCAACA ACAACAGAGC ATTTTATTCA GTAATCAGAA TACCATGGCT ACAATGGCGT 
     4921 CTCCAAAGCA ACCACCACCA AACATGATAT TCAACCCAAA TCAAAATCCA ATGGCTAATC 
     4981 AGGAGCAACA GAACCAGTCA ATTTTTCACC AACAAAGTAA CATGGCCCCA ATGAATCAAG 
     5041 AGCAACAGCC CATGCAATTT CAGAGTCAGT CCACAGTTTC CTCACTTCAG AACCCAGGTC 
     5101 CTACCCAGTC GGAATCATCA CAGACCCCCT TGTTCCATAG CTCTCCTCAG ATTCAGTTGG 
     5161 TACAAGGGTC ACCTAGTTCT CAAGAGCAGC AAGTAACTCT CTTCTTATCT CCAGCATCCA 
     5221 TGTCTGCCTT GCAGACCAGT ATAAATCAAC AAGATATGCA ACAGTCTCCT CTTTATTCCC 
     5281 CTCAGAACAA CATGCCTGGA ATTCAAGGAG CCACATCTTC GCCTCAACCA CAGGCTACTT 
     5341 TATTTCACAA CACAGCAGGA GGCACAATGA ACCAACTGCA GAATTCTCCT GGCTCATCTC 
     5401 AGCAGACATC AGGAATGTTC TTATTTGGCA TTCAAAATAA CTGTAGTCAG CTTTTAACCT 
     5461 CTGGACCAGC TACATTGCCT GATCAGTTGA TGGCCATAAG TCAGCCAGGC CAACCACAAA 
     5521 ACGAGGGCCA GCCACCTGTG ACAACACTTC TTTCTCAGCA AATGCCAGAG AATTCTCCAC 
     5581 TGGCATCCTC TATAAACACC AACCAGAACA TCGAAAAGAT TGATTTGCTT GTTTCATTGC 
     5641 AAAACCAAGG GAACAACTTG ACTGGCTCCT TTACGCGTAC GCGGCCGCTC GAGCAGAAAC 
     5701 TCATCTCAGA AGAGGATCTG GCAGCAAATG ATATCCTGGA TTACAAGGAT GACGACGATA 
     5761 AGGTTTAAAC GGCCGGCCGC GGTCATAGCT GTTTCCTGAA CAGATCCCGG GTGGCATCCC 
     5821 TGTGACCCCT CCCCAGTGCC TCTCCTGGCC CTGGAAGTTG CCACTCCAGT GCCCACCAGC 
     5881 CTTGTCCTAA TAAAATTAAG TTGCATCATT TTGTCTGACT AGGTGTCCTT CTATAATATT 
     5941 ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG GGGCAAGTTG GGAAGACAAC CTGTAGGGCC 
     6001 TGCGGGGTCT ATTGGGAACC AAGCTGGAGT GCAGTGGCAC AATCTTGGCT CACTGCAATC 
     6061 TCCGCCTCCT GGGTTCAAGC GATTCTCCTG CCTCAGCCTC CCGAGTTGTT GGGATTCCAG 
     6121 GCATGCATGA CCAGGCTCAG CTAATTTTTG TTTTTTTGGT AGAGACGGGG TTTCACCATA 
     6181 TTGGCCAGGC TGGTCTCCAA CTCCTAATCT CAGGTGATCT ACCCACCTTG GCCTCCCAAA 
     6241 TTGCTGGGAT TACAGGCGTG AACCACTGCT CCCTTCCCTG TCCTTCTGAT TTTAAAATAA 
     6301 CTATACCAGC AGGAGGACGT CCAGACACAG CATAGGCTAC CTGGCCATGC CCAACCGGTG 
     6361 GGACATTTGA GTTGCTTGCT TGGCACTGTC CTCTCATGCG TTGGGTCCAC TCAGTAGATG 
     6421 CCTGTTGAAT TGGGTACGCG GCCAGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT 
     6481 ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA 
     6541 AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC 
     6601 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA 
     6661 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC 
     6721 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC 
     6781 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA 
     6841 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC 
     6901 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG 
     6961 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG 
     7021 AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG 
     7081 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA 
     7141 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA 
     7201 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT 
     7261 CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA 
     7321 GTAACCTGAG GCTATGGCAG GGCCTGCCGC CCCGACGTTG GCTGCGAGCC CTGGGCCTTC 
     7381 ACCCGAACTT GGGGGGTGGG GTGGGGAAAA GGAAGAAACG CGGGCGTATT GGCCCCAATG 
     7441 GGGTCTCGGT GGGGTATCGA CAGAGTGCCA GCCCTGGGAC CGAACCCCGC GTTTATGAAC 
     7501 AAACGACCCA ACACCGTGCG TTTTATTCTG TCTTTTTATT GCCGTCATAG CGCGGGTTCC 
     7561 TTCCGGTATT GTCTCCTTCC GTGTTTCAGT TAGCCTCCCC CTAGGGTGGG CGAAGAACTC 
     7621 CAGCATGAGA TCCCCGCGCT GGAGGATCAT CCAGCCGGCG TCCCGGAAAA CGATTCCGAA 
     7681 GCCCAACCTT TCATAGAAGG CGGCGGTGGA ATCGAAATCT CGTGATGGCA GGTTGGGCGT 
     7741 CGCTTGGTCG GTCATTTCGA ACCCCAGAGT CCCGCTCAGA AGAACTCGTC AAGAAGGCGA 
     7801 TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG GCGATACCGT AAAGCACGAG GAAGCGGTCA 
     7861 GCCCATTCGC CGCCAAGCTC TTCAGCAATA TCACGGGTAG CCAACGCTAT GTCCTGATAG 
     7921 CGATCCGCCA CACCCAGCCG GCCACAGTCG ATGAATCCAG AAAAGCGGCC ATTTTCCACC 
     7981 ATGATATTCG GCAAGCAGGC ATCGCCATGG GTCACGACGA GATCCTCGCC GTCGGGCATG 
     8041 CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT GGCGCGAGCC CCTGATGCTC TTCGTCCAGA 
     8101 TCATCCTGAT CGACAAGACC GGCTTCCATC CGAGTACGTG CTCGCTCGAT GCGATGTTTC 
     8161 GCTTGGTGGT CGAATGGGCA GGTAGCCGGA TCAAGCGTAT GCAGCCGCCG CATTGCATCA 
     8221 GCCATGATGG ATACTTTCTC GGCAGGAGCA AGGTGAGATG ACAGGAGATC CTGCCCCGGC 
     8281 ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC GCTTCAGTGA CAACGTCGAG CACAGCTGCG 
     8341 CAAGGAACGC CCGTCGTGGC CAGCCACGAT AGCCGCGCTG CCTCGTCTTG CAGTTCATTC 
     8401 AGGGCACCGG ACAGGTCGGT CTTGACAAAA AGAACCGGGC GCCCCTGCGC TGACAGCCGG 
     8461 AACACGGCGG CATCAGAGCA GCCGATTGTC TGTTGTGCCC AGTCATAGCC GAATAGCCTC 
     8521 TCCACCCAAG CGGCCGGAGA ACCTGCGTGC AATCCATCTT GTTCAATCAT GCGAAACGAT 
     8581 CCTCATCCTG TCTCTTGATC GATCTTTGCA AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA 
     8641 CTACTTCTGG AATAGCTCAG AGGCCGAGGC GGCCTCGGCC TCTGCATAAA TAAAAAAAAT 
     8701 TAGTCAGCCA TGGGGCGGAG AATGGGCGGA ACTGGGCGGA GTTAGGGGCG GGATGGGCGG 
     8761 AGTTAGGGGC GGGACTATGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     8821 TGCTGGGGAG CCTGGGGACT TTCCACACCT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     8881 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CCTAACTGAC ACACATTCCA 
     8941 CAGCTGGTTC TTTCCGCCTC AGGACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG 
     9001 GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG 
     9061 GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGCGC CCTGTAGCGG CGCATTAAGC 
     9121 GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC 
     9181 GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT 
     9241 CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA 
     9301 AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC 
     9361 CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA 
     9421 CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT 
     9481 TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTT
//