LOCUS       RC226398                9276 bp    DNA     circular  SYN 
DEFINITION  PTPRM (Myc-DDK-tagged)-Human protein tyrosine phosphatase, receptor type, M (PTPRM), transcript variant 1
ACCESSION   NM_001105244
VERSION     NM_001105244.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5433..5524 
                     /label="Myc-DDK"  
     misc_feature    5554..6139 
                     /label="PolyA signal"  
     misc_feature    5611..5630 
                     /label="Restriction Enzyme"  
     misc_feature    6415..7087 
                     /label="ColE1"  
     misc_feature    7517..8311 
                     /label="Kan/Neo"  
     misc_feature    8346..8648 
                     /label="SV40 ori"  
     misc_feature    8734..9211 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5415..5421 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5415 
                     /label="PTPRM"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAGGGGACTT GGGACTTGCC TGGCGACTTT GGCCGGACTT TTGCTAACTG 
     1081 CGGCGGGCGA GACGTTCTCA GGTGGCTGCC TCTTTGATGA GCCGTATAGC ACATGTGGAT 
     1141 ATAGTCAATC TGAAGGTGAT GACTTCAATT GGGAGCAAGT GAACACCTTG ACTAAACCGA 
     1201 CTTCTGATCC ATGGATGCCA TCAGGTTCTT TCATGCTGGT GAATGCCTCT GGGAGACCTG 
     1261 AGGGGCAGAG AGCCCACCTG CTCTTACCCC AACTTAAAGA AAATGACACC CACTGCATCG 
     1321 ATTTTCACTA TTTTGTGTCC AGCAAGAGTA ATTCTCCTCC GGGGTTACTC AATGTCTACG 
     1381 TGAAGGTCAA TAACGGGCCA CTGGGGAATC CTATCTGGAA TATATCTGGA GACCCAACAC 
     1441 GTACATGGAA CAGGGCAGAA CTGGCCATTA GTACTTTCTG GCCTAACTTT TATCAGGTGA 
     1501 TTTTTGAAGT GATAACTTCT GGACATCAAG GCTATCTCGC TATCGATGAG GTGAAGGTGT 
     1561 TAGGACATCC ATGTACCAGG ACTCCTCACT TCCTGCGGAT TCAGAATGTG GAAGTTAATG 
     1621 CTGGCCAGTT TGCTACCTTC CAGTGCAGTG CCATCGGCAG GACCGTGGCA GGAGACAGGC 
     1681 TCTGGTTACA GGGCATTGAT GTGCGAGATG CTCCTCTGAA GGAAATCAAG GTGACCAGCT 
     1741 CCCGACGCTT CATTGCTTCA TTTAATGTTG TGAATACCAC CAAACGAGAT GCTGGAAAGT 
     1801 ACCGCTGCAT GATTCGCACT GAAGGAGGTG TTGGAATATC AAACTATGCA GAGTTGGTAG 
     1861 TTAAAGAACC ACCCGTTCCT ATTGCCCCAC CTCAGCTCGC CTCTGTAGGA GCCACCTACC 
     1921 TGTGGATACA GCTCAACGCC AACTCCATCA ATGGGGATGG GCCCATTGTG GCCCGAGAGG 
     1981 TGGAGTACTG CACGGCCAGT GGGAGCTGGA ATGACCGGCA GCCAGTCGAT TCCACGAGCT 
     2041 ATAAAATTGG ACACCTTGAC CCAGATACAG AATATGAGAT TAGTGTGCTC CTGACCAGGC 
     2101 CAGGGGAGGG TGGCACTGGC TCTCCTGGTC CAGCTCTCAG GACAAGAACA AAGTGTGCTG 
     2161 ATCCCATGCG AGGCCCAAGA AAACTAGAAG TAGTGGAGGT CAAATCTCGG CAAATCACTA 
     2221 TCCGCTGGGA GCCATTTGGA TATAATGTAA CTCGTTGCCA CAGTTATAAT CTCACTGTCC 
     2281 ACTACTGTTA CCAAGTTGGA GGACAAGAAC AAGTGCGAGA AGAAGTAAGC TGGGATACAG 
     2341 AAAACTCACA CCCTCAACAC ACGATCACTA ACCTGTCACC ATACACCAAT GTCAGTGTGA 
     2401 AACTGATCCT CATGAACCCA GAGGGCCGGA AGGAAAGCCA AGAACTCATA GTGCAGACAG 
     2461 ATGAAGACCT CCCAGGTGCT GTTCCCACTG AATCCATACA AGGAAGTACC TTTGAAGAGA 
     2521 AGATATTTCT TCAGTGGAGA GAACCAACTC AAACATATGG TGTAATCACT TTATATGAGA 
     2581 TCACCTACAA AGCAGTCAGT TCCTTTGACC CAGAAATAGA TTTATCCAAT CAGAGTGGAA 
     2641 GAGTTTCAAA GCTGGGAAAT GAAACCCATT TTCTGTTTTT TGGACTGTAT CCGGGGACCA 
     2701 CATACTCCTT TACCATCCGA GCTAGCACAG CTAAGGGTTT TGGGCCTCCA GCAACAAACC 
     2761 AGTTCACCAC CAAAATATCA GCACCCTCTA TGCCAGCTTA TGAACTTGAG ACACCTTTGA 
     2821 ATCAAACTGA CAATACCGTG ACAGTCATGC TGAAACCTGC CCACAGCAGA GGAGCACCTG 
     2881 TCAGTGTCTA TCAAATAGTT GTTGAGGAAG AACGTCCTCG AAGAACTAAA AAGACGACAG 
     2941 AAATCTTAAA GTGCTACCCA GTGCCAATTC ACTTCCAGAA TGCTTCTCTG CTGAACTCAC 
     3001 AGTACTACTT TGCTGCAGAA TTTCCTGCAG ACAGCCTCCA AGCTGCGCAG CCTTTTACAA 
     3061 TTGGTGATAA TAAGACATAT AATGGATACT GGAACACTCC CCTTCTCCCC TATAAAAGCT 
     3121 ACAGAATTTA TTTCCAAGCT GCTAGTAGAG CCAATGGGGA AACCAAAATA GACTGTGTCC 
     3181 AAGTGGCCAC AAAAGGAGCT GCCACTCCGA AACCAGTCCC AGAACCCGAG AAACAGACAG 
     3241 ACCATACAGT TAAAATTGCT GGAGTCATCG CGGGCATCTT GCTGTTCGTG ATTATATTTC 
     3301 TTGGAGTTGT GTTGGTAATG AAGAAAAGGA AACTGGCCAA GAAGCGGAAA GAGACCATGA 
     3361 GCAGCACCCG ACAGGAGATG ACTGTGATGG TGAACTCAAT GGACAAGAGC TATGCTGAGC 
     3421 AGGGCACAAA CTGCGACGAG GCTTTCTCAT TCATGGACAC GCACAATCTG AATGGGAGAT 
     3481 CTGTGTCTTC ACCATCGTCC TTCACAATGA AAACAAATAC ACTGAGCACA TCGGTGCCTA 
     3541 ATTCCTATTA CCCAGACCCA TTTGTGCCAA CTGCAATTTT AGTGCCAATA AATGATGAAA 
     3601 CCCACACAAT GGCCAGCGAT ACCAGCAGCC TGGTGCAGTC CCATACTTAC AAGAAGCGAG 
     3661 AGCCGGCCGA CGTGCCCTAT CAGACTGGGC AGCTCCACCC CGCCATCCGG GTGGCAGACC 
     3721 TCCTTCAGCA CATCACACAG ATGAAGTGTG CGGAGGGCTA CGGCTTCAAG GAGGAATACG 
     3781 AGAGCTTCTT TGAAGGGCAG TCTGCACCAT GGGACTCGGC TAAGAAAGAT GAGAACAGAA 
     3841 TGAAGAACAG ATACGGGAAT ATCATTGCAT ACGATCATTC CCGAGTGAGG CTGCAGACAA 
     3901 TAGAAGGAGA CACAAACTCA GACTATATCA ATGGCAATTA TATCGATGGT TATCATCGAC 
     3961 CCAATCATTA CATTGCTACC CAAGGGCCAA TGCAGGAAAC CATCTATGAC TTCTGGAGGA 
     4021 TGGTGTGGCA CGAAAACACT GCAAGTATCA TCATGGTGAC CAATCTTGTG GAAGTGGGAA 
     4081 GGGTCAAATG CTGCAAATAC TGGCCAGATG ACACAGAGAT ATATAAAGAC ATTAAAGTTA 
     4141 CCCTAATAGA AACAGAACTA CTGGCAGAAT ATGTGATAAG AACATTTGCT GTTGAAAAGA 
     4201 GAGGTGTGCA TGAAATCCGA GAGATCAGAC AGTTTCACTT CACTGGCTGG CCGGATCATG 
     4261 GGGTCCCCTA CCATGCCACC GGCCTGCTGG GATTCGTGCG GCAAGTCAAG TCCAAGAGCC 
     4321 CGCCCAGTGC AGGCCCACTG GTGGTGCACT GCAGTGCTGG TGCAGGGAGG ACTGGCTGTT 
     4381 TCATCGTCAT TGATATCATG TTGGACATGG CCGAAAGGGA AGGGGTCGTA GACATCTACA 
     4441 ACTGCGTCAG GGAGCTGCGG TCACGGAGGG TGAACATGGT GCAAACAGAG GAGCAGTATG 
     4501 TGTTTATCCA CGATGCGATC CTGGAAGCCT GTCTTTGTGG GGACACCTCT GTGCCTGCTT 
     4561 CCCAAGTTAG GTCTCTGTAT TATGACATGA ACAAACTGGA TCCACAGACA AACTCAAGCC 
     4621 AGATTAAAGA GGAATTCCGG ACGCTAAACA TGGTGACACC AACGCTGCGA GTAGAGGACT 
     4681 GCAGCATCGC ACTGTTGCCC CGGAACCATG AGAAAAACCG GTGCATGGAC ATCCTGCCCC 
     4741 CAGACCGCTG CCTGCCCTTC CTCATCACCA TCGATGGGGA GAGCAGCAAC TACATCAATG 
     4801 CTGCCCTCAT GGACAGCTAT AAACAGCCTT CAGCTTTTAT AGTCACCCAG CATCCTTTGC 
     4861 CAAACACAGT GAAAGACTTT TGGAGACTGG TCCTGGATTA TCACTGCACA TCCGTAGTTA 
     4921 TGCTAAATGA TGTGGATCCT GCCCAGTTGT GTCCACAGTA CTGGCCAGAA AACGGAGTAC 
     4981 ACAGACACGG CCCCATCCAG GTGGAATTTG TCTCTGCTGA CCTGGAAGAG GACATCATCA 
     5041 GCAGGATATT CCGCATTTAC AATGCCGCCA GACCCCAAGA TGGATATCGG ATGGTGCAGC 
     5101 AATTCCAGTT CCTGGGCTGG CCGATGTACA GGGACACACC AGTGTCTAAG CGCTCCTTCT 
     5161 TGAAGCTCAT TCGCCAGGTG GACAAGTGGC AAGAGGAGTA CAATGGCGGG GAAGGCCGCA 
     5221 CGGTTGTGCA CTGCTTGAAC GGGGGAGGCC GCAGTGGGAC GTTCTGCGCC ATCAGCATCG 
     5281 TATGTGAGAT GCTCCGGCAC CAGAGAACCG TGGATGTCTT TCACGCTGTG AAGACACTGA 
     5341 GGAACAACAA GCCCAACATG GTCGACCTCC TGGATCAGTA CAAGTTCTGC TACGAGGTGG 
     5401 CCCTGGAATA CTTGAATTCT GGCACGCGTA CGCGGCCGCT CGAGCAGAAA CTCATCTCAG 
     5461 AAGAGGATCT GGCAGCAAAT GATATCCTGG ATTACAAGGA TGACGACGAT AAGGTTTAAA 
     5521 CGGCCGGCCG CGGTCATAGC TGTTTCCTGA ACAGATCCCG GGTGGCATCC CTGTGACCCC 
     5581 TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT GCCACTCCAG TGCCCACCAG CCTTGTCCTA 
     5641 ATAAAATTAA GTTGCATCAT TTTGTCTGAC TAGGTGTCCT TCTATAATAT TATGGGGTGG 
     5701 AGGGGGGTGG TATGGAGCAA GGGGCAAGTT GGGAAGACAA CCTGTAGGGC CTGCGGGGTC 
     5761 TATTGGGAAC CAAGCTGGAG TGCAGTGGCA CAATCTTGGC TCACTGCAAT CTCCGCCTCC 
     5821 TGGGTTCAAG CGATTCTCCT GCCTCAGCCT CCCGAGTTGT TGGGATTCCA GGCATGCATG 
     5881 ACCAGGCTCA GCTAATTTTT GTTTTTTTGG TAGAGACGGG GTTTCACCAT ATTGGCCAGG 
     5941 CTGGTCTCCA ACTCCTAATC TCAGGTGATC TACCCACCTT GGCCTCCCAA ATTGCTGGGA 
     6001 TTACAGGCGT GAACCACTGC TCCCTTCCCT GTCCTTCTGA TTTTAAAATA ACTATACCAG 
     6061 CAGGAGGACG TCCAGACACA GCATAGGCTA CCTGGCCATG CCCAACCGGT GGGACATTTG 
     6121 AGTTGCTTGC TTGGCACTGT CCTCTCATGC GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA 
     6181 TTGGGTACGC GGCCAGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC 
     6241 CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG 
     6301 GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA 
     6361 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA 
     6421 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG 
     6481 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG 
     6541 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT 
     6601 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA 
     6661 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG 
     6721 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT 
     6781 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC 
     6841 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG 
     6901 CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG 
     6961 GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA 
     7021 GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAACCTGA 
     7081 GGCTATGGCA GGGCCTGCCG CCCCGACGTT GGCTGCGAGC CCTGGGCCTT CACCCGAACT 
     7141 TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG 
     7201 TGGGGTATCG ACAGAGTGCC AGCCCTGGGA CCGAACCCCG CGTTTATGAA CAAACGACCC 
     7261 AACACCGTGC GTTTTATTCT GTCTTTTTAT TGCCGTCATA GCGCGGGTTC CTTCCGGTAT 
     7321 TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC CCTAGGGTGG GCGAAGAACT CCAGCATGAG 
     7381 ATCCCCGCGC TGGAGGATCA TCCAGCCGGC GTCCCGGAAA ACGATTCCGA AGCCCAACCT 
     7441 TTCATAGAAG GCGGCGGTGG AATCGAAATC TCGTGATGGC AGGTTGGGCG TCGCTTGGTC 
     7501 GGTCATTTCG AACCCCAGAG TCCCGCTCAG AAGAACTCGT CAAGAAGGCG ATAGAAGGCG 
     7561 ATGCGCTGCG AATCGGGAGC GGCGATACCG TAAAGCACGA GGAAGCGGTC AGCCCATTCG 
     7621 CCGCCAAGCT CTTCAGCAAT ATCACGGGTA GCCAACGCTA TGTCCTGATA GCGATCCGCC 
     7681 ACACCCAGCC GGCCACAGTC GATGAATCCA GAAAAGCGGC CATTTTCCAC CATGATATTC 
     7741 GGCAAGCAGG CATCGCCATG GGTCACGACG AGATCCTCGC CGTCGGGCAT GCTCGCCTTG 
     7801 AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC CCCTGATGCT CTTCGTCCAG ATCATCCTGA 
     7861 TCGACAAGAC CGGCTTCCAT CCGAGTACGT GCTCGCTCGA TGCGATGTTT CGCTTGGTGG 
     7921 TCGAATGGGC AGGTAGCCGG ATCAAGCGTA TGCAGCCGCC GCATTGCATC AGCCATGATG 
     7981 GATACTTTCT CGGCAGGAGC AAGGTGAGAT GACAGGAGAT CCTGCCCCGG CACTTCGCCC 
     8041 AATAGCAGCC AGTCCCTTCC CGCTTCAGTG ACAACGTCGA GCACAGCTGC GCAAGGAACG 
     8101 CCCGTCGTGG CCAGCCACGA TAGCCGCGCT GCCTCGTCTT GCAGTTCATT CAGGGCACCG 
     8161 GACAGGTCGG TCTTGACAAA AAGAACCGGG CGCCCCTGCG CTGACAGCCG GAACACGGCG 
     8221 GCATCAGAGC AGCCGATTGT CTGTTGTGCC CAGTCATAGC CGAATAGCCT CTCCACCCAA 
     8281 GCGGCCGGAG AACCTGCGTG CAATCCATCT TGTTCAATCA TGCGAAACGA TCCTCATCCT 
     8341 GTCTCTTGAT CGATCTTTGC AAAAGCCTAG GCCTCCAAAA AAGCCTCCTC ACTACTTCTG 
     8401 GAATAGCTCA GAGGCCGAGG CGGCCTCGGC CTCTGCATAA ATAAAAAAAA TTAGTCAGCC 
     8461 ATGGGGCGGA GAATGGGCGG AACTGGGCGG AGTTAGGGGC GGGATGGGCG GAGTTAGGGG 
     8521 CGGGACTATG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     8581 GCCTGGGGAC TTTCCACACC TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     8641 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCCTAACTGA CACACATTCC ACAGCTGGTT 
     8701 CTTTCCGCCT CAGGACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT 
     8761 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC 
     8821 ACATTTCCCC GAAAAGTGCC ACCTGACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT 
     8881 GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC 
     8941 GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG 
     9001 GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT 
     9061 TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG 
     9121 TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT 
     9181 ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA 
     9241 AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTT
//