LOCUS       RC226390                9063 bp    DNA     circular  SYN 
DEFINITION  ROBO2 (Myc-DDK-tagged)-Human roundabout, axon guidance receptor, homolog 2 (Drosophila) (ROBO2), transcript variant 1
ACCESSION   NM_001128929
VERSION     NM_001128929.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5220..5311 
                     /label="Myc-DDK"  
     misc_feature    5341..5926 
                     /label="PolyA signal"  
     misc_feature    5398..5417 
                     /label="Restriction Enzyme"  
     misc_feature    6202..6874 
                     /label="ColE1"  
     misc_feature    7304..8098 
                     /label="Kan/Neo"  
     misc_feature    8133..8435 
                     /label="SV40 ori"  
     misc_feature    8521..8998 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5202..5208 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5202 
                     /label="ROBO2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCCAGAAGA CATGAACGTG TCACTAGAAG GATGTGGACA TGGGCTCCGG 
     1081 GACTGTTGAT GATGACTGTG GTGTTTTGGG GTCATCAGGG GAATGGACAA GGCCAAGGAT 
     1141 CGCGTCTTCG CCAGGAGGAC TTTCCCCCGC GGATTGTGGA GCATCCTTCC GATGTCATCG 
     1201 TCTCTAAGGG CGAGCCCACG ACTCTGAACT GCAAGGCGGA GGGCCGGCCA ACGCCCACCA 
     1261 TTGAGTGGTA CAAAGATGGG GAGCGAGTGG AGACTGACAA GGACGATCCC CGGTCCCACA 
     1321 GGATGCTTCT GCCCAGCGGA TCCTTATTCT TCTTGCGCAT CGTGCACGGG CGCAGGAGTA 
     1381 AACCTGATGA AGGAAGCTAC GTTTGTGTTG CGAGGAACTA TCTTGGTGAA GCAGTGAGTC 
     1441 GAAATGCGTC TCTGGAAGTG GCATTGTTAC GAGATGACTT CCGACAAAAC CCCACAGATG 
     1501 TTGTAGTGGC AGCTGGAGAG CCTGCAATCC TGGAGTGCCA GCCTCCCCGG GGACACCCAG 
     1561 AACCCACCAT CTACTGGAAA AAAGACAAAG TTCGAATTGA TGACAAGGAA GAAAGAATAA 
     1621 GTATCCGTGG TGGAAAACTG ATGATCTCCA ATACCAGGAA AAGTGATGCA GGGATGTATA 
     1681 CTTGTGTTGG TACCAATATG GTGGGAGAAA GGGACAGTGA CCCAGCAGAG CTGACTGTCT 
     1741 TTGAACGACC CACATTTCTC AGGAGGCCAA TTAACCAGGT GGTACTGGAG GAAGAAGCTG 
     1801 TAGAATTTCG TTGTCAAGTC CAAGGAGATC CTCAACCAAC TGTGAGGTGG AAAAAGGATG 
     1861 ATGCAGACTT GCCAAGAGGA AGGTATGACA TCAAAGACGA TTACACACTA AGAATTAAAA 
     1921 AGACCATGAG TACAGATGAA GGCACCTATA TGTGTATTGC TGAGAATCGG GTTGGAAAAA 
     1981 TGGAAGCCTC TGCTACACTC ACCGTCCGAG CTCCCCCACA GTTTGTGGTT CGGCCAAGAG 
     2041 ATCAGATTGT TGCTCAAGGT CGAACAGTGA CATTTCCCTG TGAAACTAAA GGAAACCCAC 
     2101 AGCCAGCTGT TTTTTGGCAG AAAGAAGGCA GCCAGAACCT ACTTTTCCCA AACCAACCCC 
     2161 AGCAGCCCAA CAGTAGATGC TCAGTGTCAC CAACTGGAGA CCTCACAATC ACCAACATTC 
     2221 AACGTTCCGA CGCGGGTTAC TACATCTGCC AGGCTTTAAC TGTGGCAGGA AGCATTTTAG 
     2281 CAAAAGCTCA ACTGGAGGTT ACTGATGTTT TGACAGATAG ACCTCCACCT ATAATTCTAC 
     2341 AAGGCCCAGC CAACCAAACG CTGGCAGTGG ATGGTACAGC GTTACTGAAA TGTAAAGCCA 
     2401 CTGGTGATCC TCTTCCTGTA ATTAGCTGGT TAAAGGAGGG ATTTACTTTT CCGGGTAGAG 
     2461 ATCCAAGAGC AACAATTCAA GAGCAAGGCA CACTGCAGAT TAAGAATTTA CGGATTTCTG 
     2521 ATACTGGCAC TTATACTTGT GTGGCTACAA GTTCAAGTGG AGAGACTTCC TGGAGTGCAG 
     2581 TGCTGGATGT GACAGAGTCT GGAGCAACAA TCAGTAAAAA CTATGATTTA AGTGACCTGC 
     2641 CAGGGCCACC ATCCAAACCG CAGGTCACTG ATGTTACTAA GAACAGTGTC ACCTTGTCCT 
     2701 GGCAGCCAGG TACCCCTGGA ACCCTTCCAG CAAGTGCATA TATCATTGAG GCTTTCAGCC 
     2761 AATCAGTGAG CAACAGCTGG CAGACCGTGG CAAACCATGT AAAGACCACC CTCTATACTG 
     2821 TAAGAGGACT GCGGCCCAAT ACAATCTACT TATTCATGGT CAGAGCGATC AACCCCCAAG 
     2881 GTCTCAGTGA CCCAAGTCCC ATGTCAGATC CTGTGCGCAC ACAAGATATC AGCCCACCAG 
     2941 CACAAGGAGT GGACCACAGG CAAGTGCAGA AAGAGCTAGG AGATGTCCTT GTCCGTCTTC 
     3001 ATAATCCAGT TGTGCTGACT CCCACCACGG TTCAGGTCAC ATGGACGGTT GATCGCCAAC 
     3061 CCCAGTTTAT CCAAGGCTAC CGAGTGATGT ATCGTCAGAC TTCAGGTCTG CAGGCGACAT 
     3121 CTTCGTGGCA GAATTTAGAT GCCAAAGTCC CGACTGAACG AAGTGCTGTC TTAGTCAACC 
     3181 TGAAAAAGGG GGTGACTTAT GAAATTAAAG TACGGCCATA TTTTAATGAG TTCCAAGGAA 
     3241 TGGATAGTGA ATCTAAAACG GTTCGTACTA CTGAAGAAGC CCCAAGTGCC CCACCACAGT 
     3301 CTGTCACTGT ACTGACAGTT GGAAGCTACA ATAGCACAAG TATTAGTGTT TCCTGGGATC 
     3361 CTCCTCCTCC AGATCACCAG AATGGAATTA TCCAAGAATA CAAGATCTGG TGTCTAGGAA 
     3421 ATGAAACGCG ATTCCATATC AACAAAACTG TGGATGCAGC CATTCGGTCC GTAATAATTG 
     3481 GTGGATTATT CCCAGGTATT CAATACCGGG TAGAGGTTGC AGCTAGTACC AGTGCAGGGG 
     3541 TTGGAGTAAA GAGTGAGCCA CAGCCAATAA TAATCGGGAG ACGCAATGAA GTTGTCATTA 
     3601 CTGAAAACAA TAACAGCATA ACTGAGCAAA TCACTGATGT GGTGAAGCAA CCAGCCTTTA 
     3661 TAGCTGGTAT TGGTGGTGCC TGCTGGGTAA TTCTGATGGG TTTTAGCATA TGGTTGTATT 
     3721 GGCGAAGAAA GAAGAGGAAG GGACTCAGTA ATTATGCTGT TACGTTTCAA AGAGGAGATG 
     3781 GAGGACTAAT GAGCAATGGA AGCCGTCCAG GTCTTCTCAA TGCTGGTGAT CCCAGCTATC 
     3841 CATGGCTTGC TGATTCTTGG CCAGCCACGA GCTTGCCAGT AAATAATAGC AACAGTGGCC 
     3901 CAAATGAGAT TGGAAATTTT GGCCGTGGAG ATGTGCTGCC ACCAGTTCCA GGCCAAGGGG 
     3961 ATAAAACAGC AACGATGCTC TCAGATGGAG CCATTTATAG TAGCATTGAC TTCACTACCA 
     4021 AAACCAGTTA CAACAGTTCC AGCCAAATAA CACAGGCTAC CCCATATGCC ACGACACAGA 
     4081 TCTTGCATTC CAACAGCATA CATGAATTGG CTGTCGATCT GCCTGATCCA CAATGGAAAA 
     4141 GCTCAATTCA GCAAAAAACA GATCTGATGG GATTTGGTTA TTCTCTACCT GATCAGAACA 
     4201 AAGGTAACAA TGGTGGGAAA GGTGGAAAAA AGAAGAAAAA TAAAAACTCT TCTAAACCAC 
     4261 AGAAAAACAA TGGATCCACT TGGGCCAATG TCCCTCTACC TCCCCCCCCA GTCCAGCCCC 
     4321 TTCCTGGCAC GGAGCTGGAA CACTATGCAG TGGAACAACA AGAAAATGGC TATGACAGTG 
     4381 ATAGCTGGTG CCCACCATTG CCAGTACAAA CTTACTTACA CCAAGGTCTG GAAGATGAAC 
     4441 TGGAAGAAGA TGATGATAGG GTCCCAACAC CTCCTGTTCG AGGCGTGGCT TCTTCTCCTG 
     4501 CTATCTCCTT TGGACAGCAG TCCACTGCAA CTCTTACTCC ATCCCCACGG GAAGAGATGC 
     4561 AACCCATGCT GCAGGCTCAC CTGGATGAGT TGACAAGAGC CTATCAGTTT GATATAGCAA 
     4621 AACAAACATG GCACATTCAA AGCAATAATC AACCTCCACA GCCTCCAGTT CCACCGTTAG 
     4681 GTTATGTGTC TGGAGCCTTG ATTTCTGATT TGGAAACGGA TGTTGCAGAT GATGATGCCG 
     4741 ACGACGAAGA GGAAGCTTTA GAAATCCCCA GGCCCCTGAG AGCACTGGAC CAGACTCCTG 
     4801 GATCCAGCAT GGACAATCTA GACAGCTCTG TGACAGGAAA AGCCTTTACC TCCTCTCAAA 
     4861 GACCTCGACC TACCAGCCCA TTTTCTACTG ACAGTAACAC CAGTGCAGCC CTGAGTCAAA 
     4921 GTCAGAGGCC TCGGCCCACT AAAAAACACA AGGGAGGGCG GATGGACCAA CAACCAGCAT 
     4981 TGCCTCATCG AAGGGAAGGA ATGACAGATG AGGAGGCCTT GGTGCCCTAT AGCAAGCCCA 
     5041 GTTTCCCATC TCCAGGTGGC CACAGCTCAT CAGGAACAGC TTCTTCTAAG GGATCCACTG 
     5101 GACCTAGGAA AACCGAGGTG TTGAGAGCAG GCCACCAGCG CAATGCCAGC GACCTTCTTG 
     5161 ACATAGGATA TATGGGCTCC AACAGTCAAG GACAGTTTAC AGGTGAATTA ACGCGTACGC 
     5221 GGCCGCTCGA GCAGAAACTC ATCTCAGAAG AGGATCTGGC AGCAAATGAT ATCCTGGATT 
     5281 ACAAGGATGA CGACGATAAG GTTTAAACGG CCGGCCGCGG TCATAGCTGT TTCCTGAACA 
     5341 GATCCCGGGT GGCATCCCTG TGACCCCTCC CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC 
     5401 ACTCCAGTGC CCACCAGCCT TGTCCTAATA AAATTAAGTT GCATCATTTT GTCTGACTAG 
     5461 GTGTCCTTCT ATAATATTAT GGGGTGGAGG GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG 
     5521 AAGACAACCT GTAGGGCCTG CGGGGTCTAT TGGGAACCAA GCTGGAGTGC AGTGGCACAA 
     5581 TCTTGGCTCA CTGCAATCTC CGCCTCCTGG GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC 
     5641 GAGTTGTTGG GATTCCAGGC ATGCATGACC AGGCTCAGCT AATTTTTGTT TTTTTGGTAG 
     5701 AGACGGGGTT TCACCATATT GGCCAGGCTG GTCTCCAACT CCTAATCTCA GGTGATCTAC 
     5761 CCACCTTGGC CTCCCAAATT GCTGGGATTA CAGGCGTGAA CCACTGCTCC CTTCCCTGTC 
     5821 CTTCTGATTT TAAAATAACT ATACCAGCAG GAGGACGTCC AGACACAGCA TAGGCTACCT 
     5881 GGCCATGCCC AACCGGTGGG ACATTTGAGT TGCTTGCTTG GCACTGTCCT CTCATGCGTT 
     5941 GGGTCCACTC AGTAGATGCC TGTTGAATTG GGTACGCGGC CAGCGGCGAG CGGTATCAGC 
     6001 TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT 
     6061 GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT 
     6121 CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG 
     6181 AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC 
     6241 TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT 
     6301 GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA 
     6361 GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA 
     6421 TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA 
     6481 CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA 
     6541 CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT 
     6601 CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT 
     6661 TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT 
     6721 CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT 
     6781 GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC 
     6841 AATCTAAAGT ATATATGAGT AACCTGAGGC TATGGCAGGG CCTGCCGCCC CGACGTTGGC 
     6901 TGCGAGCCCT GGGCCTTCAC CCGAACTTGG GGGGTGGGGT GGGGAAAAGG AAGAAACGCG 
     6961 GGCGTATTGG CCCCAATGGG GTCTCGGTGG GGTATCGACA GAGTGCCAGC CCTGGGACCG 
     7021 AACCCCGCGT TTATGAACAA ACGACCCAAC ACCGTGCGTT TTATTCTGTC TTTTTATTGC 
     7081 CGTCATAGCG CGGGTTCCTT CCGGTATTGT CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT 
     7141 AGGGTGGGCG AAGAACTCCA GCATGAGATC CCCGCGCTGG AGGATCATCC AGCCGGCGTC 
     7201 CCGGAAAACG ATTCCGAAGC CCAACCTTTC ATAGAAGGCG GCGGTGGAAT CGAAATCTCG 
     7261 TGATGGCAGG TTGGGCGTCG CTTGGTCGGT CATTTCGAAC CCCAGAGTCC CGCTCAGAAG 
     7321 AACTCGTCAA GAAGGCGATA GAAGGCGATG CGCTGCGAAT CGGGAGCGGC GATACCGTAA 
     7381 AGCACGAGGA AGCGGTCAGC CCATTCGCCG CCAAGCTCTT CAGCAATATC ACGGGTAGCC 
     7441 AACGCTATGT CCTGATAGCG ATCCGCCACA CCCAGCCGGC CACAGTCGAT GAATCCAGAA 
     7501 AAGCGGCCAT TTTCCACCAT GATATTCGGC AAGCAGGCAT CGCCATGGGT CACGACGAGA 
     7561 TCCTCGCCGT CGGGCATGCT CGCCTTGAGC CTGGCGAACA GTTCGGCTGG CGCGAGCCCC 
     7621 TGATGCTCTT CGTCCAGATC ATCCTGATCG ACAAGACCGG CTTCCATCCG AGTACGTGCT 
     7681 CGCTCGATGC GATGTTTCGC TTGGTGGTCG AATGGGCAGG TAGCCGGATC AAGCGTATGC 
     7741 AGCCGCCGCA TTGCATCAGC CATGATGGAT ACTTTCTCGG CAGGAGCAAG GTGAGATGAC 
     7801 AGGAGATCCT GCCCCGGCAC TTCGCCCAAT AGCAGCCAGT CCCTTCCCGC TTCAGTGACA 
     7861 ACGTCGAGCA CAGCTGCGCA AGGAACGCCC GTCGTGGCCA GCCACGATAG CCGCGCTGCC 
     7921 TCGTCTTGCA GTTCATTCAG GGCACCGGAC AGGTCGGTCT TGACAAAAAG AACCGGGCGC 
     7981 CCCTGCGCTG ACAGCCGGAA CACGGCGGCA TCAGAGCAGC CGATTGTCTG TTGTGCCCAG 
     8041 TCATAGCCGA ATAGCCTCTC CACCCAAGCG GCCGGAGAAC CTGCGTGCAA TCCATCTTGT 
     8101 TCAATCATGC GAAACGATCC TCATCCTGTC TCTTGATCGA TCTTTGCAAA AGCCTAGGCC 
     8161 TCCAAAAAAG CCTCCTCACT ACTTCTGGAA TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC 
     8221 TGCATAAATA AAAAAAATTA GTCAGCCATG GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT 
     8281 TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG GACTATGGTT GCTGACTAAT TGAGATGCAT 
     8341 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCTGG TTGCTGACTA 
     8401 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCC 
     8461 TAACTGACAC ACATTCCACA GCTGGTTCTT TCCGCCTCAG GACTCTTCCT TTTTCAATAT 
     8521 TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG 
     8581 AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGCGCCC 
     8641 TGTAGCGGCG CATTAAGCGC GGCGGGTGTG GTGGTTACGC GCAGCGTGAC CGCTACACTT 
     8701 GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC 
     8761 GGCTTTCCCC GTCAAGCTCT AAATCGGGGG CTCCCTTTAG GGTTCCGATT TAGTGCTTTA 
     8821 CGGCACCTCG ACCCCAAAAA ACTTGATTAG GGTGATGGTT CACGTAGTGG GCCATCGCCC 
     8881 TGATAGACGG TTTTTCGCCC TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG 
     8941 TTCCAAACTG GAACAACACT CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT 
     9001 TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT 
     9061 TTT
//