LOCUS       RC226389                8970 bp    DNA     circular  SYN 
DEFINITION  PRG4 (Myc-DDK-tagged)-Human proteoglycan 4 (PRG4), transcript variant B. Note: ORF is codon optimized
ACCESSION   NM_001127708
VERSION     NM_001127708.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5127..5218 
                     /label="Myc-DDK"  
     misc_feature    5248..5833 
                     /label="PolyA signal"  
     misc_feature    5305..5324 
                     /label="Restriction Enzyme"  
     misc_feature    6109..6781 
                     /label="ColE1"  
     misc_feature    7211..8005 
                     /label="Kan/Neo"  
     misc_feature    8040..8342 
                     /label="SV40 ori"  
     misc_feature    8428..8905 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5109..5115 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5109 
                     /label="PRG4"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCTTGGAAG ACCCTGCCAA TTTATCTGCT CCTTCTGCTG AGCGTCTTTG 
     1081 TGATACAGCA GGTGAGTAGT CAGGAGCTCT CATGCAAGGG CAGGTGTTTC GAGTCTTTCG 
     1141 AGCGCGGACG GGAGTGCGAT TGCGACGCTC AGTGCAAAAA GTATGACAAG TGCTGCCCTG 
     1201 ACTACGAAAG CTTTTGCGCG GAGGTTCACA ACCCCACATC TCCGCCCTCA AGCAAAAAGG 
     1261 CCCCACCGCC TTCCGGCGCC TCCCAGACCA TTAAGAGTAC AACCAAAAGG AGCCCTAAAC 
     1321 CACCAAACAA GAAAAAAACA AAAAAAGTTA TAGAGTCAGA GGAAATCACC GAAGAGCATA 
     1381 GTGTCTCCGA GAACCAGGAA AGTTCAAGCT CATCCAGCTC CTCCTCATCA TCAAGTACCA 
     1441 TCCGAAAAAT CAAGAGCTCC AAAAATTCCG CCGCAAATAG AGAGTTGCAG AAGAAGCTGA 
     1501 AAGTGAAGGA TAACAAAAAA AACAGAACAA AAAAGAAACC CACCCCTAAA CCACCGGTTG 
     1561 TGGATGAGGC CGGCTCTGGG CTGGACAATG GAGACTTTAA AGTCACCACT CCAGACACGT 
     1621 CAACCACACA ACACAACAAA GTAAGCACCT CACCAAAGAT AACCACAGCC AAGCCCATTA 
     1681 ATCCACGGCC ATCCTTGCCT CCTAACAGCG ACACATCAAA AGAAACTTCT TTGACCGTGA 
     1741 ACAAGGAAAC CACGGTCGAG ACCAAGGAAA CTACAACCAC TAATAAGCAG ACCTCCACCG 
     1801 ACGGGAAGGA GAAGACCACT TCAGCTAAAG AGACACAGTC CATAGAGAAA ACTTCTGCAA 
     1861 AAGATCTGGC ACCAACGTCT AAGGTGCTCG CTAAACCGAC TCCGAAAGCA GAAACCACCA 
     1921 CAAAGGGTCC CGCTCTCACT ACTCCAAAGG AGCCAACACC TACCACACCC AAAGAACCCG 
     1981 CTTCAACCAC ACCTAAGGAG CCCACGCCCA CCACAATTAA GTCCGCCCCG ACTACACCCA 
     2041 AGGAGCCTGC CCCGACCACA ACAAAATCTG CCCCTACTAC CCCGAAGGAG CCCGCTCCAA 
     2101 CAACCACTAA GGAGCCCGCA CCGACTACCC CTAAGGAACC CGCCCCTACG ACGACAAAGG 
     2161 AACCAGCTCC AACCACGACC AAGAGCGCTC CCACCACCCC TAAGGAGCCT GCCCCCACAA 
     2221 CTCCTAAGAA GCCGGCTCCT ACAACCCCGA AGGAACCAGC CCCAACCACG CCAAAAGAAC 
     2281 CCACTCCAAC AACCCCTAAA GAGCCAGCAC CTACAACTAA AGAGCCTGCT CCGACAACCC 
     2341 CTAAGGAGCC TGCTCCTACA GCCCCCAAAA AACCAGCGCC CACCACGCCC AAGGAGCCCG 
     2401 CTCCTACAAC CCCCAAAGAA CCAGCCCCAA CCACTACTAA GGAACCGTCA CCCACTACCC 
     2461 CAAAAGAACC GGCACCCACT ACCACAAAAT CAGCCCCCAC TACCACAAAA GAGCCAGCGC 
     2521 CAACCACTAC CAAAAGTGCG CCTACTACAC CGAAAGAGCC TAGCCCAACC ACTACAAAAG 
     2581 AACCGGCGCC CACAACTCCC AAAGAACCCG CTCCGACCAC CCCTAAGAAA CCAGCACCTA 
     2641 CTACACCCAA GGAACCCGCC CCTACCACAC CAAAAGAGCC AGCCCCCACC ACAACGAAAA 
     2701 AACCCGCGCC TACCACACCC AAAGAACCCG CCCCCACTAC TCCAAAGGAG ACCGCTCCTA 
     2761 CCACGCCAAA GAAACTGACC CCCACTACGC CGGAGAAACT GGCACCAACC ACCCCAGAGA 
     2821 AACCCGCCCC CACTACTCCC GAGGAACTCG CTCCGACGAC ACCCGAAGAA CCCACGCCTA 
     2881 CAACGCCTGA GGAGCCTGCC CCTACAACCC CAAAAGCCGC AGCGCCAAAT ACCCCCAAGG 
     2941 AACCTGCGCC GACTACTCCT AAGGAGCCCG CACCCACAAC ACCAAAGGAG CCAGCGCCGA 
     3001 CAACTCCTAA AGAGACCGCT CCAACTACGC CTAAAGGGAC TGCACCTACG ACATTGAAAG 
     3061 AACCTGCGCC TACGACCCCT AAGAAACCTG CTCCCAAGGA ACTCGCTCCC ACTACCACAA 
     3121 AAGAGCCAAC GTCCACCACA TGCGATAAAC CAGCCCCGAC CACTCCCAAG GGTACGGCTC 
     3181 CAACCACACC CAAGGAACCT GCCCCAACTA CACCGAAGGA GCCCGCCCCA ACGACCCCAA 
     3241 AGGGGACTGC TCCTACTACG TTGAAAGAAC CAGCCCCCAC AACACCAAAA AAACCCGCCC 
     3301 CCAAAGAGCT CGCCCCTACA ACTACAAAGG GCCCTACTAG TACCACCAGT GACAAACCCG 
     3361 CACCAACAAC CCCGAAAGAG ACTGCCCCTA CCACACCTAA GGAGCCGGCG CCTACCACCC 
     3421 CCAAAAAACC TGCCCCGACA ACCCCTGAAA CCCCTCCCCC AACAACAAGC GAGGTGTCAA 
     3481 CCCCCACGAC TACCAAGGAG CCCACTACCA TTCACAAATC TCCTGATGAG AGCACCCCAG 
     3541 AGTTGAGTGC GGAGCCCACT CCGAAAGCTC TGGAAAATAG TCCAAAAGAG CCAGGTGTCC 
     3601 CCACTACAAA GACTCCTGCT GCTACCAAGC CCGAAATGAC AACTACCGCC AAGGATAAAA 
     3661 CCACCGAGCG AGATCTGCGG ACAACACCCG AGACTACTAC AGCCGCACCA AAAATGACGA 
     3721 AAGAAACAGC CACAACCACC GAAAAAACCA CAGAGTCCAA GATAACCGCC ACCACCACGC 
     3781 AAGTGACATC TACCACAACA CAGGATACTA CGCCCTTCAA AATCACCACA CTTAAAACAA 
     3841 CTACACTGGC ACCAAAGGTG ACAACCACTA AGAAGACAAT AACAACGACT GAGATAATGA 
     3901 ATAAGCCCGA AGAGACAGCG AAACCAAAGG ACAGAGCTAC CAATTCCAAA GCCACCACCC 
     3961 CAAAGCCACA AAAACCAACA AAGGCACCCA AAAAGCCTAC CAGTACCAAA AAACCAAAGA 
     4021 CCATGCCTAG AGTACGGAAG CCCAAGACAA CACCCACACC GCGCAAAATG ACGAGCACTA 
     4081 TGCCAGAACT TAATCCCACA TCCCGGATCG CAGAAGCTAT GTTGCAGACC ACTACCCGCC 
     4141 CCAATCAGAC ACCGAACAGT AAACTCGTCG AAGTCAACCC CAAATCAGAG GATGCCGGAG 
     4201 GCGCCGAAGG GGAGACTCCA CATATGCTCC TGCGACCGCA TGTTTTTATG CCTGAAGTGA 
     4261 CCCCTGACAT GGATTACCTT CCTAGGGTCC CTAATCAGGG TATCATCATC AATCCTATGC 
     4321 TCAGTGATGA GACCAATATT TGTAACGGGA AACCAGTGGA TGGACTGACA ACACTGCGCA 
     4381 ACGGCACACT GGTTGCTTTT CGCGGTCATT ATTTTTGGAT GCTGTCCCCA TTCTCACCAC 
     4441 CATCCCCAGC TCGAAGGATC ACAGAGGTGT GGGGCATTCC TTCTCCAATT GACACCGTTT 
     4501 TCACCCGCTG CAATTGCGAA GGAAAAACCT TCTTTTTCAA AGACAGTCAG TATTGGCGAT 
     4561 TTACAAATGA TATCAAAGAC GCAGGGTATC CCAAGCCGAT CTTCAAAGGC TTCGGAGGCC 
     4621 TGACAGGCCA GATTGTTGCC GCTCTCTCCA CCGCTAAGTA CAAGAACTGG CCTGAATCCG 
     4681 TGTATTTCTT TAAGAGAGGG GGAAGCATTC AACAATATAT CTATAAGCAG GAGCCCGTGC 
     4741 AGAAATGCCC AGGCAGACGG CCCGCTCTGA ACTACCCAGT TTACGGCGAG ACTACCCAGG 
     4801 TAAGGAGGAG GCGCTTCGAG AGAGCTATTG GGCCCTCCCA AACCCACACA ATACGGATAC 
     4861 AGTACTCTCC GGCAAGACTG GCATACCAGG ACAAGGGAGT GCTCCACAAC GAGGTAAAGG 
     4921 TTAGCATATT GTGGCGGGGC TTGCCAAACG TTGTAACATC TGCCATAAGC CTGCCCAACA 
     4981 TTCGCAAGCC TGACGGATAC GACTACTATG CATTTAGCAA AGATCAGTAT TACAATATAG 
     5041 ACGTGCCTTC CAGAACTGCC CGCGCAATTA CCACCAGGTC CGGCCAGACA CTCTCCAAGG 
     5101 TGTGGTATAA TTGCCCTACG CGTACGCGGC CGCTCGAGCA GAAACTCATC TCAGAAGAGG 
     5161 ATCTGGCAGC AAATGATATC CTGGATTACA AGGATGACGA CGATAAGGTT TAAACGGCCG 
     5221 GCCGCGGTCA TAGCTGTTTC CTGAACAGAT CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA 
     5281 GTGCCTCTCC TGGCCCTGGA AGTTGCCACT CCAGTGCCCA CCAGCCTTGT CCTAATAAAA 
     5341 TTAAGTTGCA TCATTTTGTC TGACTAGGTG TCCTTCTATA ATATTATGGG GTGGAGGGGG 
     5401 GTGGTATGGA GCAAGGGGCA AGTTGGGAAG ACAACCTGTA GGGCCTGCGG GGTCTATTGG 
     5461 GAACCAAGCT GGAGTGCAGT GGCACAATCT TGGCTCACTG CAATCTCCGC CTCCTGGGTT 
     5521 CAAGCGATTC TCCTGCCTCA GCCTCCCGAG TTGTTGGGAT TCCAGGCATG CATGACCAGG 
     5581 CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA CGGGGTTTCA CCATATTGGC CAGGCTGGTC 
     5641 TCCAACTCCT AATCTCAGGT GATCTACCCA CCTTGGCCTC CCAAATTGCT GGGATTACAG 
     5701 GCGTGAACCA CTGCTCCCTT CCCTGTCCTT CTGATTTTAA AATAACTATA CCAGCAGGAG 
     5761 GACGTCCAGA CACAGCATAG GCTACCTGGC CATGCCCAAC CGGTGGGACA TTTGAGTTGC 
     5821 TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG TCCACTCAGT AGATGCCTGT TGAATTGGGT 
     5881 ACGCGGCCAG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA 
     5941 ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG 
     6001 TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA 
     6061 AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT 
     6121 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT 
     6181 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT 
     6241 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC 
     6301 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT 
     6361 ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC 
     6421 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT 
     6481 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA 
     6541 ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA 
     6601 AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA 
     6661 AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT 
     6721 TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAC CTGAGGCTAT 
     6781 GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC GAGCCCTGGG CCTTCACCCG AACTTGGGGG 
     6841 GTGGGGTGGG GAAAAGGAAG AAACGCGGGC GTATTGGCCC CAATGGGGTC TCGGTGGGGT 
     6901 ATCGACAGAG TGCCAGCCCT GGGACCGAAC CCCGCGTTTA TGAACAAACG ACCCAACACC 
     6961 GTGCGTTTTA TTCTGTCTTT TTATTGCCGT CATAGCGCGG GTTCCTTCCG GTATTGTCTC 
     7021 CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG GTGGGCGAAG AACTCCAGCA TGAGATCCCC 
     7081 GCGCTGGAGG ATCATCCAGC CGGCGTCCCG GAAAACGATT CCGAAGCCCA ACCTTTCATA 
     7141 GAAGGCGGCG GTGGAATCGA AATCTCGTGA TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT 
     7201 TTCGAACCCC AGAGTCCCGC TCAGAAGAAC TCGTCAAGAA GGCGATAGAA GGCGATGCGC 
     7261 TGCGAATCGG GAGCGGCGAT ACCGTAAAGC ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA 
     7321 AGCTCTTCAG CAATATCACG GGTAGCCAAC GCTATGTCCT GATAGCGATC CGCCACACCC 
     7381 AGCCGGCCAC AGTCGATGAA TCCAGAAAAG CGGCCATTTT CCACCATGAT ATTCGGCAAG 
     7441 CAGGCATCGC CATGGGTCAC GACGAGATCC TCGCCGTCGG GCATGCTCGC CTTGAGCCTG 
     7501 GCGAACAGTT CGGCTGGCGC GAGCCCCTGA TGCTCTTCGT CCAGATCATC CTGATCGACA 
     7561 AGACCGGCTT CCATCCGAGT ACGTGCTCGC TCGATGCGAT GTTTCGCTTG GTGGTCGAAT 
     7621 GGGCAGGTAG CCGGATCAAG CGTATGCAGC CGCCGCATTG CATCAGCCAT GATGGATACT 
     7681 TTCTCGGCAG GAGCAAGGTG AGATGACAGG AGATCCTGCC CCGGCACTTC GCCCAATAGC 
     7741 AGCCAGTCCC TTCCCGCTTC AGTGACAACG TCGAGCACAG CTGCGCAAGG AACGCCCGTC 
     7801 GTGGCCAGCC ACGATAGCCG CGCTGCCTCG TCTTGCAGTT CATTCAGGGC ACCGGACAGG 
     7861 TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC TGCGCTGACA GCCGGAACAC GGCGGCATCA 
     7921 GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA TAGCCGAATA GCCTCTCCAC CCAAGCGGCC 
     7981 GGAGAACCTG CGTGCAATCC ATCTTGTTCA ATCATGCGAA ACGATCCTCA TCCTGTCTCT 
     8041 TGATCGATCT TTGCAAAAGC CTAGGCCTCC AAAAAAGCCT CCTCACTACT TCTGGAATAG 
     8101 CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC ATAAATAAAA AAAATTAGTC AGCCATGGGG 
     8161 CGGAGAATGG GCGGAACTGG GCGGAGTTAG GGGCGGGATG GGCGGAGTTA GGGGCGGGAC 
     8221 TATGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     8281 GGACTTTCCA CACCTGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     8341 TGGGGAGCCT GGGGACTTTC CACACCCTAA CTGACACACA TTCCACAGCT GGTTCTTTCC 
     8401 GCCTCAGGAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG 
     8461 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT 
     8521 CCCCGAAAAG TGCCACCTGA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG 
     8581 GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC 
     8641 TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC 
     8701 CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT 
     8761 GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG 
     8821 TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG 
     8881 GTCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG 
     8941 CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//