LOCUS       RC226377                8724 bp    DNA     circular  SYN 
DEFINITION  GRIN2A (Myc-DDK-tagged)-Human glutamate receptor, ionotropic, N-methyl D-aspartate 2A (GRIN2A), transcript variant 3
ACCESSION   NM_001134408
VERSION     NM_001134408.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4881..4972 
                     /label="Myc-DDK"  
     misc_feature    5002..5587 
                     /label="PolyA signal"  
     misc_feature    5059..5078 
                     /label="Restriction Enzyme"  
     misc_feature    5863..6535 
                     /label="ColE1"  
     misc_feature    6965..7759 
                     /label="Kan/Neo"  
     misc_feature    7794..8096 
                     /label="SV40 ori"  
     misc_feature    8182..8659 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4863..4869 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4863 
                     /label="GRIN2A"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGCAGAGTG GGCTATTGGA CCCTGCTGGT GCTGCCGGCC CTTCTGGTCT 
     1081 GGCGCGGTCC GGCGCCGAGC GCGGCGGCGG AGAAGGGTCC CCCCGCGCTA AATATTGCGG 
     1141 TGATGCTGGG TCACAGCCAC GACGTGACAG AGCGCGAACT TCGAACACTG TGGGGCCCCG 
     1201 AGCAGGCGGC GGGGCTGCCC CTGGACGTGA ACGTGGTAGC TCTGCTGATG AACCGCACCG 
     1261 ACCCCAAGAG CCTCATCACG CACGTGTGCG ACCTCATGTC CGGGGCACGC ATCCACGGCC 
     1321 TCGTGTTTGG GGACGACACG GACCAGGAGG CCGTAGCCCA GATGCTGGAT TTTATCTCCT 
     1381 CCCACACCTT CGTCCCCATC TTGGGCATTC ATGGGGGCGC ATCTATGATC ATGGCTGACA 
     1441 AGGATCCGAC GTCTACCTTC TTCCAGTTTG GAGCGTCCAT CCAGCAGCAA GCCACGGTCA 
     1501 TGCTGAAGAT CATGCAGGAT TATGACTGGC ATGTCTTCTC CCTGGTGACC ACTATCTTCC 
     1561 CTGGCTACAG GGAATTCATC AGCTTCGTCA AGACCACAGT GGACAACAGC TTTGTGGGCT 
     1621 GGGACATGCA GAATGTGATC ACACTGGACA CTTCCTTTGA GGATGCAAAG ACACAAGTCC 
     1681 AGCTGAAGAA GATCCACTCT TCTGTCATCT TGCTCTACTG TTCCAAAGAC GAGGCTGTTC 
     1741 TCATTCTGAG TGAGGCCCGC TCCCTTGGCC TCACCGGGTA TGATTTCTTC TGGATTGTCC 
     1801 CCAGCTTGGT CTCTGGGAAC ACGGAGCTCA TCCCAAAAGA GTTTCCATCG GGACTCATTT 
     1861 CTGTCTCCTA CGATGACTGG GACTACAGCC TGGAGGCGAG AGTGAGGGAC GGCATTGGCA 
     1921 TCCTAACCAC CGCTGCATCT TCTATGCTGG AGAAGTTCTC CTACATCCCC GAGGCCAAGG 
     1981 CCAGCTGCTA CGGGCAGATG GAGAGGCCAG AGGTCCCGAT GCACACCTTG CACCCATTTA 
     2041 TGGTCAATGT TACATGGGAT GGCAAAGACT TATCCTTCAC TGAGGAAGGC TACCAGGTGC 
     2101 ACCCCAGGCT GGTGGTGATT GTGCTGAACA AAGACCGGGA ATGGGAAAAG GTGGGCAAGT 
     2161 GGGAGAACCA TACGCTGAGC CTGAGGCACG CCGTGTGGCC CAGGTACAAG TCCTTCTCCG 
     2221 ACTGTGAGCC GGATGACAAC CATCTCAGCA TCGTCACCCT GGAGGAGGCC CCATTCGTCA 
     2281 TCGTGGAAGA CATAGACCCC CTGACCGAGA CGTGTGTGAG GAACACCGTG CCATGTCGGA 
     2341 AGTTCGTCAA AATCAACAAT TCAACCAATG AGGGGATGAA TGTGAAGAAA TGCTGCAAGG 
     2401 GGTTCTGCAT TGATATTCTG AAGAAGCTTT CCAGAACTGT GAAGTTTACT TACGACCTCT 
     2461 ATCTGGTGAC CAATGGGAAG CATGGCAAGA AAGTTAACAA TGTGTGGAAT GGAATGATCG 
     2521 GTGAAGTGGT CTATCAACGG GCAGTCATGG CAGTTGGCTC GCTCACCATC AATGAGGAAC 
     2581 GTTCTGAAGT GGTGGACTTC TCTGTGCCCT TTGTGGAAAC GGGAATCAGT GTCATGGTTT 
     2641 CAAGAAGTAA TGGCACCGTC TCACCTTCTG CTTTTCTAGA ACCATTCAGC GCCTCTGTCT 
     2701 GGGTGATGAT GTTTGTGATG CTGCTCATTG TTTCTGCCAT AGCTGTTTTT GTCTTTGAAT 
     2761 ACTTCAGCCC TGTTGGATAC AACAGAAACT TAGCCAAAGG GAAAGCACCC CATGGGCCTT 
     2821 CTTTTACAAT TGGAAAAGCT ATATGGCTTC TTTGGGGCCT GGTGTTCAAT AACTCCGTGC 
     2881 CTGTCCAGAA TCCTAAAGGG ACCACCAGCA AGATCATGGT ATCTGTATGG GCCTTCTTCG 
     2941 CTGTCATATT CCTGGCTAGC TACACAGCCA ATCTGGCTGC CTTCATGATC CAAGAGGAAT 
     3001 TTGTGGACCA AGTGACCGGC CTCAGTGACA AAAAGTTTCA GAGACCTCAT GACTATTCCC 
     3061 CACCTTTTCG ATTTGGGACA GTGCCTAATG GAAGCACGGA GAGAAACATT CGGAATAACT 
     3121 ATCCCTACAT GCATCAGTAC ATGACCAAAT TTAATCAGAA AGGAGTAGAG GACGCCTTGG 
     3181 TCAGCCTGAA AACGGGGAAG CTGGACGCTT TCATCTACGA TGCCGCAGTC TTGAATTACA 
     3241 AGGCTGGGAG GGATGAAGGC TGCAAGCTGG TGACCATCGG GAGTGGGTAC ATCTTTGCCA 
     3301 CCACCGGTTA TGGAATTGCC CTTCAGAAAG GCTCTCCTTG GAAGAGGCAG ATCGACCTGG 
     3361 CCTTGCTTCA GTTTGTGGGT GATGGTGAGA TGGAGGAGCT GGAGACCCTG TGGCTCACTG 
     3421 GGATCTGCCA CAACGAGAAG AACGAGGTGA TGAGCAGCCA GCTGGACATT GACAACATGG 
     3481 CGGGCGTATT CTACATGCTG GCTGCCGCCA TGGCCCTTAG CCTCATCACC TTCATCTGGG 
     3541 AGCACCTCTT CTACTGGAAG CTGCGCTTCT GTTTCACGGG CGTGTGCTCC GACCGGCCTG 
     3601 GGTTGCTCTT CTCCATCAGC AGGGGCATCT ACAGCTGCAT TCATGGAGTG CACATTGAAG 
     3661 AAAAGAAGAA GTCTCCAGAC TTCAATCTGA CGGGATCCCA GAGCAACATG TTAAAACTCC 
     3721 TCCGGTCAGC CAAAAACATT TCCAGCATGT CCAACATGAA CTCCTCAAGA ATGGACTCAC 
     3781 CCAAAAGAGC TGCTGACTTC ATCCAAAGAG GTTCCCTCAT CATGGACATG GTTTCAGATA 
     3841 AGGGGAATTT GATGTACTCA GACAACAGGT CCTTTCAGGG GAAAGAGAGC ATTTTTGGAG 
     3901 ACAACATGAA CGAACTCCAA ACATTTGTGG CCAACCGGCA GAAGGATAAC CTCAATAACT 
     3961 ATGTATTCCA GGGACAACAT CCTCTTACTC TCAATGAGTC CAACCCTAAC ACGGTGGAGG 
     4021 TGGCCGTGAG CACAGAATCC AAAGCGAACT CTAGACCCCG GCAGCTGTGG AAGAAATCCG 
     4081 TGGATTCCAT ACGCCAGGAT TCACTATCCC AGAATCCAGT CTCCCAGAGG GATGAGGCAA 
     4141 CAGCAGAGAA TAGGACCCAC TCCCTAAAGA GCCCTAGGTA TCTTCCAGAA GAGATGGCCC 
     4201 ACTCTGACAT TTCAGAAACG TCAAATCGGG CCACGTGCCA CAGGGAACCT GACAACAGTA 
     4261 AGAACCACAA AACCAAGGAC AACTTTAAAA GGTCAGTGGC CTCCAAATAC CCCAAGGACT 
     4321 GTAGTGAGGT CGAGCGCACC TACCTGAAAA CCAAATCAAG CTCCCCTAGA GACAAGATCT 
     4381 ACACTATAGA TGGTGAGAAG GAGCCTGGTT TCCACTTAGA TCCACCCCAG TTTGTTGAAA 
     4441 ATGTGACCCT GCCCGAGAAC GTGGACTTCC CGGACCCCTA CCAGGATCCC AGTGAAAACT 
     4501 TCCGCAAGGG GGACTCCACG CTGCCAATGA ACCGGAACCC CTTGCATAAT GAAGAGGGGC 
     4561 TTTCCAACAA CGACCAGTAT AAACTCTACT CCAAGCACTT CACCTTGAAA GACAAGGGTT 
     4621 CCCCGCACAG TGAGACCAGC GAGCGATACC GGCAGAACTC CACGCACTGC AGAAGCTGCC 
     4681 TTTCCAACAT GCCCACCTAT TCAGGCCACT TCACCATGAG GTCCCCCTTC AAGTGCGATG 
     4741 CCTGCCTGCG GATGGGGAAC CTCTATGACA TCGATGAAGA CCAGATGCTT CAGGAGACAG 
     4801 GGATGACCAA CGCTTGGTTA TTGGGAGATG CCCCTCGGAC CCTTACAAAC ACTCGTTGCC 
     4861 ATCCCAGGCG GACGCGTACG CGGCCGCTCG AGCAGAAACT CATCTCAGAA GAGGATCTGG 
     4921 CAGCAAATGA TATCCTGGAT TACAAGGATG ACGACGATAA GGTTTAAACG GCCGGCCGCG 
     4981 GTCATAGCTG TTTCCTGAAC AGATCCCGGG TGGCATCCCT GTGACCCCTC CCCAGTGCCT 
     5041 CTCCTGGCCC TGGAAGTTGC CACTCCAGTG CCCACCAGCC TTGTCCTAAT AAAATTAAGT 
     5101 TGCATCATTT TGTCTGACTA GGTGTCCTTC TATAATATTA TGGGGTGGAG GGGGGTGGTA 
     5161 TGGAGCAAGG GGCAAGTTGG GAAGACAACC TGTAGGGCCT GCGGGGTCTA TTGGGAACCA 
     5221 AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT CCGCCTCCTG GGTTCAAGCG 
     5281 ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG CATGCATGAC CAGGCTCAGC 
     5341 TAATTTTTGT TTTTTTGGTA GAGACGGGGT TTCACCATAT TGGCCAGGCT GGTCTCCAAC 
     5401 TCCTAATCTC AGGTGATCTA CCCACCTTGG CCTCCCAAAT TGCTGGGATT ACAGGCGTGA 
     5461 ACCACTGCTC CCTTCCCTGT CCTTCTGATT TTAAAATAAC TATACCAGCA GGAGGACGTC 
     5521 CAGACACAGC ATAGGCTACC TGGCCATGCC CAACCGGTGG GACATTTGAG TTGCTTGCTT 
     5581 GGCACTGTCC TCTCATGCGT TGGGTCCACT CAGTAGATGC CTGTTGAATT GGGTACGCGG 
     5641 CCAGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG 
     5701 GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA 
     5761 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG 
     5821 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC 
     5881 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC 
     5941 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC 
     6001 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG 
     6061 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC 
     6121 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA 
     6181 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC 
     6241 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC 
     6301 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG 
     6361 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC 
     6421 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA 
     6481 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAACCTGAGG CTATGGCAGG 
     6541 GCCTGCCGCC CCGACGTTGG CTGCGAGCCC TGGGCCTTCA CCCGAACTTG GGGGGTGGGG 
     6601 TGGGGAAAAG GAAGAAACGC GGGCGTATTG GCCCCAATGG GGTCTCGGTG GGGTATCGAC 
     6661 AGAGTGCCAG CCCTGGGACC GAACCCCGCG TTTATGAACA AACGACCCAA CACCGTGCGT 
     6721 TTTATTCTGT CTTTTTATTG CCGTCATAGC GCGGGTTCCT TCCGGTATTG TCTCCTTCCG 
     6781 TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC GAAGAACTCC AGCATGAGAT CCCCGCGCTG 
     6841 GAGGATCATC CAGCCGGCGT CCCGGAAAAC GATTCCGAAG CCCAACCTTT CATAGAAGGC 
     6901 GGCGGTGGAA TCGAAATCTC GTGATGGCAG GTTGGGCGTC GCTTGGTCGG TCATTTCGAA 
     6961 CCCCAGAGTC CCGCTCAGAA GAACTCGTCA AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA 
     7021 TCGGGAGCGG CGATACCGTA AAGCACGAGG AAGCGGTCAG CCCATTCGCC GCCAAGCTCT 
     7081 TCAGCAATAT CACGGGTAGC CAACGCTATG TCCTGATAGC GATCCGCCAC ACCCAGCCGG 
     7141 CCACAGTCGA TGAATCCAGA AAAGCGGCCA TTTTCCACCA TGATATTCGG CAAGCAGGCA 
     7201 TCGCCATGGG TCACGACGAG ATCCTCGCCG TCGGGCATGC TCGCCTTGAG CCTGGCGAAC 
     7261 AGTTCGGCTG GCGCGAGCCC CTGATGCTCT TCGTCCAGAT CATCCTGATC GACAAGACCG 
     7321 GCTTCCATCC GAGTACGTGC TCGCTCGATG CGATGTTTCG CTTGGTGGTC GAATGGGCAG 
     7381 GTAGCCGGAT CAAGCGTATG CAGCCGCCGC ATTGCATCAG CCATGATGGA TACTTTCTCG 
     7441 GCAGGAGCAA GGTGAGATGA CAGGAGATCC TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG 
     7501 TCCCTTCCCG CTTCAGTGAC AACGTCGAGC ACAGCTGCGC AAGGAACGCC CGTCGTGGCC 
     7561 AGCCACGATA GCCGCGCTGC CTCGTCTTGC AGTTCATTCA GGGCACCGGA CAGGTCGGTC 
     7621 TTGACAAAAA GAACCGGGCG CCCCTGCGCT GACAGCCGGA ACACGGCGGC ATCAGAGCAG 
     7681 CCGATTGTCT GTTGTGCCCA GTCATAGCCG AATAGCCTCT CCACCCAAGC GGCCGGAGAA 
     7741 CCTGCGTGCA ATCCATCTTG TTCAATCATG CGAAACGATC CTCATCCTGT CTCTTGATCG 
     7801 ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA GCCTCCTCAC TACTTCTGGA ATAGCTCAGA 
     7861 GGCCGAGGCG GCCTCGGCCT CTGCATAAAT AAAAAAAATT AGTCAGCCAT GGGGCGGAGA 
     7921 ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG GATGGGCGGA GTTAGGGGCG GGACTATGGT 
     7981 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     8041 TCCACACCTG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     8101 GCCTGGGGAC TTTCCACACC CTAACTGACA CACATTCCAC AGCTGGTTCT TTCCGCCTCA 
     8161 GGACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA 
     8221 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA 
     8281 AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG 
     8341 CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT 
     8401 TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA 
     8461 GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT 
     8521 TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG 
     8581 TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT 
     8641 TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT 
     8701 TAACAAAAAT TTAACGCGAA TTTT
//