LOCUS       RC226364                8562 bp    DNA     circular  SYN 
DEFINITION  RBM20 (Myc-DDK-tagged)-Human RNA binding motif protein 20 (RBM20)
ACCESSION   NM_001134363
VERSION     NM_001134363.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4719..4810 
                     /label="Myc-DDK"  
     misc_feature    4840..5425 
                     /label="PolyA signal"  
     misc_feature    4897..4916 
                     /label="Restriction Enzyme"  
     misc_feature    5701..6373 
                     /label="ColE1"  
     misc_feature    6803..7597 
                     /label="Kan/Neo"  
     misc_feature    7632..7934 
                     /label="SV40 ori"  
     misc_feature    8020..8497 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4701..4707 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4701 
                     /label="RBM20"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGTGCTGGCA GCAGCCATGA GCCAGGACGC GGACCCCAGC GGTCCGGAGC 
     1081 AGCCGGACAG AGTTGCCTGC AGTGTGCCTG GTGCCCGGGC GTCCCCGGCA CCCTCCGGCC 
     1141 CGCGAGGGAT GCAGCAGCCG CCGCCGCCGC CCCAGCCACC GCCCCCGCCC CAAGCCGGCC 
     1201 TACCCCAGAT CATCCAAAAT GCCGCCAAGC TCCTGGACAA GAACCCATTC TCGGTCAGTA 
     1261 ACCCGAACCC TCTGCTTCCT TCACCTGCCA GTCTCCAGCT GGCTCAACTG CAGGCCCAGC 
     1321 TCACCCTCCA CCGGCTGAAG CTGGCACAGA CAGCTGTCAC CAACAACACT GCAGCCGCCA 
     1381 CAGTCCTGAA CCAAGTCCTC TCCAAAGTGG CCATGTCCCA GCCTCTCTTC AATCAACTGA 
     1441 GGCATCCGTC TGTGATCACT GGCCCCCACG GCCATGCTGG GGTTCCCCAA CATGCTGCAG 
     1501 CCATACCCAG TACCCGGTTT CCCTCTAATG CAATTGCCTT TTCACCCCCC AGCCAGACAC 
     1561 GAGGCCCCGG ACCCTCCATG AACCTTCCCA ACCAGCCACC CAGTGCCATG GTGATGCATC 
     1621 CTTTCACTGG GGTAATGCCT CAGACCCCTG GCCAGCCAGC AGTCATCTTG GGCATTGGCA 
     1681 AGACTGGGCC TGCTCCAGCT ACAGCAGGAT TCTATGAGTA TGGCAAAGCC AGCTCTGGCC 
     1741 AGACATATGG CCCTGAAACA GATGGTCAGC CTGGCTTCCT GCCATCCTCG GCCTCAACCT 
     1801 CGGGCAGTGT GACCTATGAA GGGCACTACA GCCACACAGG GCAGGATGGT CAAGCTGCCT 
     1861 TTTCCAAAGA TTTTTACGGA CCCAACTCCC AAGGTTCACA TGTGGCCAGC GGATTTCCAG 
     1921 CTGAGCAGGC TGGGGGCCTG AAAAGTGAGG TCGGGCCACT GCTGCAGGGC ACAAACAGCC 
     1981 AATGGGAGAG CCCCCATGGA TTCTCGGGCC AAAGCAAGCC TGATCTCACA GCAGGTCCCA 
     2041 TGTGGCCTCC ACCCCACAAC CAGCCCTATG AGCTGTACGA CCCCGAGGAA CCAACCTCAG 
     2101 ACAGGACACC TCCTTCCTTC GGGGGTCGGC TTAACAACAG CAAACAGGGT TTTATCGGTG 
     2161 CTGGGCGGAG GGCCAAGGAG GACCAGGCGT TGCTATCTGT GCGGCCCCTG CAGGCTCATG 
     2221 AGCTGAACGA CTTTCACGGT GTGGCCCCCC TCCACTTGCC GCATATCTGT AGCATCTGTG 
     2281 ACAAGAAGGT GTTTGATTTG AAGGACTGGG AGCTGCATGT GAAAGGGAAG CTGCACGCTC 
     2341 AGAAATGCCT GGTCTTCTCT GAAAATGCTG GCATCCGGTG TATACTTGGT TCGGCAGAGG 
     2401 GAACATTGTG TGCTTCTCCC AACAGCACAG CTGTTTATAA CCCTGCTGGG AATGAAGATT 
     2461 ATGCCTCAAA TCTTGGAACA TCATACGTGC CCATTCCAGC AAGGTCATTC ACTCAGTCAA 
     2521 GCCCCACATT TCCTTTGGCT TCTGTGGGGA CAACTTTTGC ACAGCGGAAA GGGGCTGGCC 
     2581 GTGTGGTGCA CATCTGCAAT CTCCCTGAAG GAAGCTGCAC TGAGAATGAC GTCATTAACC 
     2641 TGGGGCTGCC CTTTGGAAAG GTCACTAATT ACATCCTCAT GAAGTCGACT AATCAGGCCT 
     2701 TTTTAGAGAT GGCTTACACA GAAGCTGCAC AGGCCATGGT CCAGTATTAT CAAGAAAAAT 
     2761 CTGCTGTGAT CAATGGTGAG AAGTTGCTCA TTCGGATGTC CAAGAGATAC AAGGAATTGC 
     2821 AGCTCAAGAA ACCCGGGAAG GCCGTGGCTG CCATCATCCA GGACATCCAT TCCCAGAGGG 
     2881 AGAGGGACAT GTTCCGGGAA GCAGACAGAT ATGGCCCAGA AAGGCCGCGG TCTCGTAGTC 
     2941 CGGTGAGCCG GTCACTCTCC CCGAGGTCCC ACACTCCCAG CTTCACCTCC TGCAGCTCTT 
     3001 CCCACAGCCC TCCGGGCCCC TCCCGGGCTG ACTGGGGCAA TGGCCGGGAC TCCTGGGAGC 
     3061 ACTCTCCCTA TGCCAGGAGG GAGGAAGAGC GAGACCCGGC TCCCTGGAGG GACAACGGAG 
     3121 ATGACAAGAG GGACAGGATG GACCCCTGGG CACATGATCG CAAACACCAC CCCCGGCAAC 
     3181 TGGACAAGGC TGAGTTGGAC GAGCGACCAG AAGGAGGGAG GCCCCACCGG GAGAAGTACC 
     3241 CGAGATCTGG GTCTCCCAAC CTGCCCCACT CTGTGTCCAG CTACAAAAGC CGTGAAGACG 
     3301 GCTACTACCG GAAAGAGCCC AAAGCCAAGT GGGACAAGTA TCTGAAGCAG CAGCAGGATG 
     3361 CCCCCGGGAG GTCCAGGAGG AAAGACGAGG CCAGGCTGCG GGAAAGCAGA CACCCCCATC 
     3421 CGGATGACTC AGGCAAGGAA GATGGGCTGG GGCCAAAGGT CACTAGGGCC CCTGAGGGCG 
     3481 CCAAGGCCAA GCAGAATGAG AAAAATAAAA CCAAGAGAAC TGATAGAGAC CAAGAAGGAG 
     3541 CTGATGATAG AAAAGAAAAC ACAATGGCAG AGAATGAGGC TGGAAAAGAG GAACAGGAGG 
     3601 GCATGGAAGA AAGCCCTCAA TCAGTGGGCA GACAGGAGAA AGAAGCAGAG TTCTCTGATC 
     3661 CGGAAAACAC AAGGACAAAG AAGGAACAAG ATTGGGAGAG TGAAAGTGAG GCAGAGGGGG 
     3721 AGAGCTGGTA TCCCACTAAC ATGGAGGAGC TGGTGACAGT GGACGAGGTT GGGGAAGAAG 
     3781 AAGATTTTAT CGTGGAACCA GACATCCCAG AGCTGGAAGA AATTGTGCCC ATTGACCAGA 
     3841 AAGACAAAAT TTGCCCAGAA ACATGTCTGT GTGTGACAAC CACCTTAGAC TTAGACCTGG 
     3901 CCCAGGATTT CCCCAAGGAA GGAGTCAAGG CCGTAGGGAA TGGGGCTGCA GAAATCAGCC 
     3961 TCAAGTCACC CAGAGAACTG CCCTCTGCTT CCACAAGCTG TCCCAGTGAC ATGGACGTGG 
     4021 AAATGCCTGG CCTAAATCTG GATGCTGAGC GGAAGCCAGC TGAAAGTGAG ACAGGCCTCT 
     4081 CCCTGGAGGA TTCAGATTGC TACGAGAAGG AGGCAAAGGG AGTGGAGAGC TCAGATGTTC 
     4141 ATCCAGCCCC TACAGTCCAG CAAATGTCTT CCCCTAAGCC AGCAGAGGAG AGGGCCCGGC 
     4201 AGCCAAGCCC ATTTGTGGAT GATTGCAAGA CCAGGGGGAC CCCCGAAGAT GGGGCTTGTG 
     4261 AAGGCAGCCC CCTGGAGGAG AAAGCCAGCC CCCCCATCGA AACTGACCTC CAAAACCAAG 
     4321 CTTGCCAAGA AGTGTTGACC CCGGAAAACT CCAGGTACGT GGAAATGAAA TCTCTGGAGG 
     4381 TGAGGTCACC AGAGTACACT GAAGTGGAAC TGAAACAGCC CCTTTCTTTG CCCTCTTGGG 
     4441 AACCAGAGGA TGTGTTCAGT GAACTTAGCA TTCCTCTAGG GGTGGAGTTC GTGGTTCCCA 
     4501 GGACTGGCTT TTATTGCAAG CTGTGTGGGC TGTTCTACAC GAGCGAGGAG ACAGCAAAGA 
     4561 TGAGCCACTG CCGCAGCGCT GTCCACTACA GGAACTTACA GAAATATTTG TCCCAGCTGG 
     4621 CCGAGGAGGG CCTCAAGGAG ACCGAGGGGG CAGATAGCCC GAGGCCAGAG GACAGCGGAA 
     4681 TCGTGCCACG CTTCGAAAGG AAAAAGCTCA CGCGTACGCG GCCGCTCGAG CAGAAACTCA 
     4741 TCTCAGAAGA GGATCTGGCA GCAAATGATA TCCTGGATTA CAAGGATGAC GACGATAAGG 
     4801 TTTAAACGGC CGGCCGCGGT CATAGCTGTT TCCTGAACAG ATCCCGGGTG GCATCCCTGT 
     4861 GACCCCTCCC CAGTGCCTCT CCTGGCCCTG GAAGTTGCCA CTCCAGTGCC CACCAGCCTT 
     4921 GTCCTAATAA AATTAAGTTG CATCATTTTG TCTGACTAGG TGTCCTTCTA TAATATTATG 
     4981 GGGTGGAGGG GGGTGGTATG GAGCAAGGGG CAAGTTGGGA AGACAACCTG TAGGGCCTGC 
     5041 GGGGTCTATT GGGAACCAAG CTGGAGTGCA GTGGCACAAT CTTGGCTCAC TGCAATCTCC 
     5101 GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA 
     5161 TGCATGACCA GGCTCAGCTA ATTTTTGTTT TTTTGGTAGA GACGGGGTTT CACCATATTG 
     5221 GCCAGGCTGG TCTCCAACTC CTAATCTCAG GTGATCTACC CACCTTGGCC TCCCAAATTG 
     5281 CTGGGATTAC AGGCGTGAAC CACTGCTCCC TTCCCTGTCC TTCTGATTTT AAAATAACTA 
     5341 TACCAGCAGG AGGACGTCCA GACACAGCAT AGGCTACCTG GCCATGCCCA ACCGGTGGGA 
     5401 CATTTGAGTT GCTTGCTTGG CACTGTCCTC TCATGCGTTG GGTCCACTCA GTAGATGCCT 
     5461 GTTGAATTGG GTACGCGGCC AGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG 
     5521 GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA 
     5581 GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA 
     5641 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG 
     5701 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT 
     5761 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG 
     5821 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC 
     5881 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT 
     5941 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA 
     6001 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC 
     6061 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC 
     6121 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT 
     6181 TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC 
     6241 TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT 
     6301 CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA 
     6361 ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC GACGTTGGCT GCGAGCCCTG GGCCTTCACC 
     6421 CGAACTTGGG GGGTGGGGTG GGGAAAAGGA AGAAACGCGG GCGTATTGGC CCCAATGGGG 
     6481 TCTCGGTGGG GTATCGACAG AGTGCCAGCC CTGGGACCGA ACCCCGCGTT TATGAACAAA 
     6541 CGACCCAACA CCGTGCGTTT TATTCTGTCT TTTTATTGCC GTCATAGCGC GGGTTCCTTC 
     6601 CGGTATTGTC TCCTTCCGTG TTTCAGTTAG CCTCCCCCTA GGGTGGGCGA AGAACTCCAG 
     6661 CATGAGATCC CCGCGCTGGA GGATCATCCA GCCGGCGTCC CGGAAAACGA TTCCGAAGCC 
     6721 CAACCTTTCA TAGAAGGCGG CGGTGGAATC GAAATCTCGT GATGGCAGGT TGGGCGTCGC 
     6781 TTGGTCGGTC ATTTCGAACC CCAGAGTCCC GCTCAGAAGA ACTCGTCAAG AAGGCGATAG 
     6841 AAGGCGATGC GCTGCGAATC GGGAGCGGCG ATACCGTAAA GCACGAGGAA GCGGTCAGCC 
     6901 CATTCGCCGC CAAGCTCTTC AGCAATATCA CGGGTAGCCA ACGCTATGTC CTGATAGCGA 
     6961 TCCGCCACAC CCAGCCGGCC ACAGTCGATG AATCCAGAAA AGCGGCCATT TTCCACCATG 
     7021 ATATTCGGCA AGCAGGCATC GCCATGGGTC ACGACGAGAT CCTCGCCGTC GGGCATGCTC 
     7081 GCCTTGAGCC TGGCGAACAG TTCGGCTGGC GCGAGCCCCT GATGCTCTTC GTCCAGATCA 
     7141 TCCTGATCGA CAAGACCGGC TTCCATCCGA GTACGTGCTC GCTCGATGCG ATGTTTCGCT 
     7201 TGGTGGTCGA ATGGGCAGGT AGCCGGATCA AGCGTATGCA GCCGCCGCAT TGCATCAGCC 
     7261 ATGATGGATA CTTTCTCGGC AGGAGCAAGG TGAGATGACA GGAGATCCTG CCCCGGCACT 
     7321 TCGCCCAATA GCAGCCAGTC CCTTCCCGCT TCAGTGACAA CGTCGAGCAC AGCTGCGCAA 
     7381 GGAACGCCCG TCGTGGCCAG CCACGATAGC CGCGCTGCCT CGTCTTGCAG TTCATTCAGG 
     7441 GCACCGGACA GGTCGGTCTT GACAAAAAGA ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC 
     7501 ACGGCGGCAT CAGAGCAGCC GATTGTCTGT TGTGCCCAGT CATAGCCGAA TAGCCTCTCC 
     7561 ACCCAAGCGG CCGGAGAACC TGCGTGCAAT CCATCTTGTT CAATCATGCG AAACGATCCT 
     7621 CATCCTGTCT CTTGATCGAT CTTTGCAAAA GCCTAGGCCT CCAAAAAAGC CTCCTCACTA 
     7681 CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC CTCGGCCTCT GCATAAATAA AAAAAATTAG 
     7741 TCAGCCATGG GGCGGAGAAT GGGCGGAACT GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT 
     7801 TAGGGGCGGG ACTATGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     7861 TGGGGAGCCT GGGGACTTTC CACACCTGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     7921 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCCT AACTGACACA CATTCCACAG 
     7981 CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT 
     8041 TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT 
     8101 CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGCGCCCT GTAGCGGCGC ATTAAGCGCG 
     8161 GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT 
     8221 CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA 
     8281 AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA 
     8341 CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT 
     8401 TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC 
     8461 AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG 
     8521 TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TT
//