LOCUS       RC226362                8511 bp    DNA     circular  SYN 
DEFINITION  PHKA1 (Myc-DDK-tagged)-Human phosphorylase kinase, alpha 1 (muscle) (PHKA1), transcript variant 2
ACCESSION   NM_001122670
VERSION     NM_001122670.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4668..4759 
                     /label="Myc-DDK"  
     misc_feature    4789..5374 
                     /label="PolyA signal"  
     misc_feature    4846..4865 
                     /label="Restriction Enzyme"  
     misc_feature    5650..6322 
                     /label="ColE1"  
     misc_feature    6752..7546 
                     /label="Kan/Neo"  
     misc_feature    7581..7883 
                     /label="SV40 ori"  
     misc_feature    7969..8446 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4650..4656 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4650 
                     /label="PHKA1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAGGAGCCGG AGTAACTCCG GGGTCCGGCT GGACGGCTAC GCTCGACTGG 
     1081 TGCAACAGAC CATCCTGTGC CATCAGAATC CAGTGACTGG CTTGCTTCCA GCCAGCTATG 
     1141 ATCAGAAAGA TGCTTGGGTC CGAGATAATG TGTACAGCAT CTTGGCTGTG TGGGGTTTGG 
     1201 GCCTGGCCTA TCGGAAGAAT GCAGACCGGG ATGAGGATAA GGCAAAGGCC TATGAATTGG 
     1261 AGCAGAGTGT AGTGAAGCTG ATGAGAGGAC TACTGCACTG CATGATCAGA CAGGTGGATA 
     1321 AAGTAGAATC CTTCAAATAT AGTCAGAGTA CTAAGGATAG CCTCCATGCA AAGTACAACA 
     1381 CCAAAACCTG TGCCACTGTA GTGGGTGATG ATCAATGGGG ACACCTGCAG TTGGATGCTA 
     1441 CCTCTGTGTA CCTGCTCTTC TTAGCCCAAA TGACTGCCTC AGGACTCCAT ATCATCCACA 
     1501 GCCTAGATGA AGTCAATTTC ATACAGAACC TTGTGTTTTA CATTGAAGCT GCATATAAAA 
     1561 CTGCTGACTT CGGGATATGG GAACGTGGAG ACAAGACCAA CCAAGGGATC TCAGAGTTGA 
     1621 ATGCCAGTTC AGTTGGAATG GCAAAGGCAG CCCTGGAAGC ATTAGATGAA CTGGATCTGT 
     1681 TTGGTGTGAA AGGTGGGCCT CAATCAGTTA TCCATGTCCT GGCTGATGAA GTACAGCACT 
     1741 GCCAGTCTAT CCTAAATTCA CTACTGCCCC GTGCTTCAAC ATCAAAAGAG GTTGATGCTA 
     1801 GTCTACTCTC AGTGGTTTCC TTCCCTGCCT TTGCAGTAGA GGATAGCCAG TTGGTGGAGC 
     1861 TCACAAAACA GGAAATCATC ACCAAGCTTC AGGGTCGTTA TGGTTGCTGT CGCTTTCTAC 
     1921 GAGATGGATA TAAAACTCCT AAAGAGGATC CCAATCGTCT GTACTATGAA CCAGCTGAGC 
     1981 TGAAGCTATT TGAAAACATT GAGTGTGAAT GGCCATTGTT CTGGACATAC TTTATTCTTG 
     2041 ATGGGGTCTT CAGTGGCAAT GCAGAACAGG TTCAAGAATA TAAAGAGGCT CTTGAAGCAG 
     2101 TCCTCATCAA GGGCAAAAAT GGAGTCCCAC TTCTGCCAGA GCTGTACAGT GTTCCTCCTG 
     2161 ACAGGGTCGA TGAAGAATAT CAGAATCCTC ACACTGTGGA CCGAGTCCCC ATGGGGAAAT 
     2221 TGCCTCACAT GTGGGGTCAG TCTCTATACA TTTTAGGAAG CTTGATGGCA GAGGGATTTT 
     2281 TAGCCCCTGG AGAAATTGAT CCCCTGAATC GCAGGTTTTC TACTGTACCG AAGCCCGATG 
     2341 TTGTGGTTCA AGTCTCCATT CTAGCTGAAA CAGAAGAAAT CAAGACCATT TTGAAGGACA 
     2401 AGGGAATTTA CGTGGAGACC ATTGCTGAGG TATACCCCAT CAGAGTACAA CCAGCTCGTA 
     2461 TTCTCAGCCA CATTTATTCC AGCCTAGGAT GCAACAATAG AATGAAACTC AGTGGACGAC 
     2521 CCTACAGACA CATGGGAGTG CTTGGAACTT CAAAACTCTA TGACATTCGG AAAACTATCT 
     2581 TTACTTTCAC TCCACAGTTT ATAGACCAGC AACAGTTCTA CCTGGCTCTG GACAACAAGA 
     2641 TGATAGTGGA AATGCTTAGA ACAGACCTCT CCTACCTCTG TAGCCGCTGG CGGATGACAG 
     2701 GCCAGCCCAC CATCACCTTC CCCATCTCAC ACAGCATGCT TGATGAAGAT GGAACAAGCT 
     2761 TGAATTCAAG TATCCTGGCA GCACTCCGAA AAATGCAAGA TGGGTATTTT GGTGGGGCAA 
     2821 GGGTTCAAAC AGGTAAATTG TCAGAGTTTT TGACAACATC TTGTTGCACA CACTTGAGCT 
     2881 TCATGGACCC TGGACCTGAG GGTAAGCTGT ACAGTGAAGA TTATGATGAC AACTATGATT 
     2941 ACCTGGAATC TGGCAACTGG ATGAATGATT ATGATTCAAC CAGTCATGCT CGCTGTGGTG 
     3001 ATGAAGTTGC TCGTTATTTA GATCACCTTT TGGCGCACAC TGCTCCCCAT CCTAAACTAG 
     3061 CCCCTACCTC ACAGAAGGGA GGGCTAGATC GGTTCCAAGC TGCTGTGCAA ACAACCTGCG 
     3121 ACTTAATGTC CTTGGTGACC AAGGCCAAGG AACTGCATGT ACAGAATGTT CACATGTATC 
     3181 TTCCTACGAA GTTATTTCAG GCTTCCCGGC CTTCATTCAA CTTACTTGAT TCACCTCATC 
     3241 CCCGACAGGA GAACCAGGTT CCCTCTGTTC GTGTAGAAAT ACATCTTCCT AGAGACCAGT 
     3301 CTGGGGAGGT GGACTTTAAA GCACTGGTTT TACAGTTGAA GGAGACCTCA AGCTTACAGG 
     3361 AACAAGCTGA TATCCTCTAT ATGCTGTATA CTATGAAAGG ACCTGACTGG AACACTGAAT 
     3421 TGTATAATGA ACGGAGTGCT ACAGTGAGAG AGCTTCTTAC CGAGCTGTAT GGCAAAGTGG 
     3481 GAGAAATTCG TCACTGGGGC CTGATCCGAT ACATTTCTGG GATCTTAAGG AAGAAAGTGG 
     3541 AAGCACTTGA TGAGGCCTGC ACAGACCTTC TCTCCCACCA GAAACATTTG ACAGTAGGAC 
     3601 TTCCTCCAGA ACCTCGAGAA AAGACTATCT CTGCACCTCT GCCCTATGAG GCGCTCACTC 
     3661 AGCTGATAGA TGAAGCCAGT GAAGGGGATA TGAGCATTTC AATCCTTACA CAGGAAATAA 
     3721 TGGTATATCT AGCCATGTAT ATGCGAACCC AGCCTGGCCT CTTTGCTGAA ATGTTTCGAC 
     3781 TTCGAATTGG TCTGATCATA CAAGTTATGG CAACAGAACT GGCCCACTCC CTTCGATGCT 
     3841 CAGCTGAGGA AGCCACAGAG GGCCTGATGA ATCTCAGTCC TTCGGCCATG AAGAATCTCC 
     3901 TGCATCACAT TCTCAGCGGC AAGGAGTTTG GAGTGGAACG AAGCGTTCGT CCCACTGATT 
     3961 CAAATGTCAG TCCTGCTATT TCTATCCACG AGATTGGTGC TGTTGGAGCA ACCAAAACAG 
     4021 AACGAACTGG GATCATGCAG TTAAAAAGTG AGATAAAGCA GTCACCTGGA ACCTCTATGA 
     4081 CTCCAAGTAG TGGGTCCTTT CCTAGTGCAT ATGATCAGCA GTCATCTAAA GATAGTCGTC 
     4141 AAGGTCAATG GCAACGCCGA AGAAGGCTGG ATGGGGCACT GAATAGAGTT CCAGTTGGAT 
     4201 TTTATCAGAA AGTATGGAAA GTTTTGCAGA AGTGTCACGG ACTTTCTGTT GAAGGGTTTG 
     4261 TCCTTCCTTC CTCTACCACT AGAGAGATGA CTCCAGGTGA GATTAAATTC TCTGTTCATG 
     4321 TGGAGTCTGT CCTGAATCGT GTACCTCAGC CAGAGTACCG TCAGCTGCTG GTTGAAGCCA 
     4381 TCCTTGTCCT CACCATGCTG GCAGATATTG AAATTCATAG CATCGGAAGC ATCATTGCTG 
     4441 TGGAAAAAAT AGTGCATATT GCCAATGACT TGTTCCTTCA AGAACAGAAA ACCCTTGGCG 
     4501 CAGATGATAC CATGTTGGCA AAGGATCCCG CATCTGGCAT CTGTACTCTT CTGTATGACA 
     4561 GTGCACCCAG TGGCAGGTTT GGCACCATGA CCTACCTCTC CAAGGCAGCC GCCACCTACG 
     4621 TGCAGGAGTT CCTGCCCCAC AGCATCTGTG CCATGCAAAC GCGTACGCGG CCGCTCGAGC 
     4681 AGAAACTCAT CTCAGAAGAG GATCTGGCAG CAAATGATAT CCTGGATTAC AAGGATGACG 
     4741 ACGATAAGGT TTAAACGGCC GGCCGCGGTC ATAGCTGTTT CCTGAACAGA TCCCGGGTGG 
     4801 CATCCCTGTG ACCCCTCCCC AGTGCCTCTC CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC 
     4861 ACCAGCCTTG TCCTAATAAA ATTAAGTTGC ATCATTTTGT CTGACTAGGT GTCCTTCTAT 
     4921 AATATTATGG GGTGGAGGGG GGTGGTATGG AGCAAGGGGC AAGTTGGGAA GACAACCTGT 
     4981 AGGGCCTGCG GGGTCTATTG GGAACCAAGC TGGAGTGCAG TGGCACAATC TTGGCTCACT 
     5041 GCAATCTCCG CCTCCTGGGT TCAAGCGATT CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA 
     5101 TTCCAGGCAT GCATGACCAG GCTCAGCTAA TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC 
     5161 ACCATATTGG CCAGGCTGGT CTCCAACTCC TAATCTCAGG TGATCTACCC ACCTTGGCCT 
     5221 CCCAAATTGC TGGGATTACA GGCGTGAACC ACTGCTCCCT TCCCTGTCCT TCTGATTTTA 
     5281 AAATAACTAT ACCAGCAGGA GGACGTCCAG ACACAGCATA GGCTACCTGG CCATGCCCAA 
     5341 CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC ACTGTCCTCT CATGCGTTGG GTCCACTCAG 
     5401 TAGATGCCTG TTGAATTGGG TACGCGGCCA GCGGCGAGCG GTATCAGCTC ACTCAAAGGC 
     5461 GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG 
     5521 CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG 
     5581 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG 
     5641 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC 
     5701 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA 
     5761 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT 
     5821 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC 
     5881 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG 
     5941 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC 
     6001 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT 
     6061 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA 
     6121 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG 
     6181 GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA 
     6241 AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT 
     6301 ATATGAGTAA CCTGAGGCTA TGGCAGGGCC TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG 
     6361 GCCTTCACCC GAACTTGGGG GGTGGGGTGG GGAAAAGGAA GAAACGCGGG CGTATTGGCC 
     6421 CCAATGGGGT CTCGGTGGGG TATCGACAGA GTGCCAGCCC TGGGACCGAA CCCCGCGTTT 
     6481 ATGAACAAAC GACCCAACAC CGTGCGTTTT ATTCTGTCTT TTTATTGCCG TCATAGCGCG 
     6541 GGTTCCTTCC GGTATTGTCT CCTTCCGTGT TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA 
     6601 GAACTCCAGC ATGAGATCCC CGCGCTGGAG GATCATCCAG CCGGCGTCCC GGAAAACGAT 
     6661 TCCGAAGCCC AACCTTTCAT AGAAGGCGGC GGTGGAATCG AAATCTCGTG ATGGCAGGTT 
     6721 GGGCGTCGCT TGGTCGGTCA TTTCGAACCC CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA 
     6781 AGGCGATAGA AGGCGATGCG CTGCGAATCG GGAGCGGCGA TACCGTAAAG CACGAGGAAG 
     6841 CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA GCAATATCAC GGGTAGCCAA CGCTATGTCC 
     6901 TGATAGCGAT CCGCCACACC CAGCCGGCCA CAGTCGATGA ATCCAGAAAA GCGGCCATTT 
     6961 TCCACCATGA TATTCGGCAA GCAGGCATCG CCATGGGTCA CGACGAGATC CTCGCCGTCG 
     7021 GGCATGCTCG CCTTGAGCCT GGCGAACAGT TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG 
     7081 TCCAGATCAT CCTGATCGAC AAGACCGGCT TCCATCCGAG TACGTGCTCG CTCGATGCGA 
     7141 TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA GCCGGATCAA GCGTATGCAG CCGCCGCATT 
     7201 GCATCAGCCA TGATGGATAC TTTCTCGGCA GGAGCAAGGT GAGATGACAG GAGATCCTGC 
     7261 CCCGGCACTT CGCCCAATAG CAGCCAGTCC CTTCCCGCTT CAGTGACAAC GTCGAGCACA 
     7321 GCTGCGCAAG GAACGCCCGT CGTGGCCAGC CACGATAGCC GCGCTGCCTC GTCTTGCAGT 
     7381 TCATTCAGGG CACCGGACAG GTCGGTCTTG ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC 
     7441 AGCCGGAACA CGGCGGCATC AGAGCAGCCG ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT 
     7501 AGCCTCTCCA CCCAAGCGGC CGGAGAACCT GCGTGCAATC CATCTTGTTC AATCATGCGA 
     7561 AACGATCCTC ATCCTGTCTC TTGATCGATC TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC 
     7621 TCCTCACTAC TTCTGGAATA GCTCAGAGGC CGAGGCGGCC TCGGCCTCTG CATAAATAAA 
     7681 AAAAATTAGT CAGCCATGGG GCGGAGAATG GGCGGAACTG GGCGGAGTTA GGGGCGGGAT 
     7741 GGGCGGAGTT AGGGGCGGGA CTATGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     7801 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCTGGTT GCTGACTAAT TGAGATGCAT 
     7861 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCCTA ACTGACACAC 
     7921 ATTCCACAGC TGGTTCTTTC CGCCTCAGGA CTCTTCCTTT TTCAATATTA TTGAAGCATT 
     7981 TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA 
     8041 ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGCGCCCTG TAGCGGCGCA 
     8101 TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA 
     8161 GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT 
     8221 CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC 
     8281 CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT 
     8341 TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA 
     8401 ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG 
     8461 GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT T
//