LOCUS       RC226360                8493 bp    DNA     circular  SYN 
DEFINITION  FNDC3B (Myc-DDK-tagged)-Human fibronectin type III domain containing 3B (FNDC3B), transcript variant 2
ACCESSION   NM_001135095
VERSION     NM_001135095.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4650..4741 
                     /label="Myc-DDK"  
     misc_feature    4771..5356 
                     /label="PolyA signal"  
     misc_feature    4828..4847 
                     /label="Restriction Enzyme"  
     misc_feature    5632..6304 
                     /label="ColE1"  
     misc_feature    6734..7528 
                     /label="Kan/Neo"  
     misc_feature    7563..7865 
                     /label="SV40 ori"  
     misc_feature    7951..8428 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4632..4638 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4632 
                     /label="FNDC3B"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTACGTCACA ATGATGATGA CCGACCAAAT CCCTCTGGAA CTGCCACCAT 
     1081 TGCTGAACGG AGAGGTAGCC ATGATGCCCC ACTTGGTGAA TGGAGATGCA GCTCAGCAGG 
     1141 TTATTCTCGT TCAAGTTAAT CCAGGTGAGA CTTTCACAAT AAGAGCAGAG GATGGAACAC 
     1201 TTCAGTGCAT TCAAGGACCT GCTGAAGTTC CCATGATGTC ACCCAATGGA TCCATTCCTC 
     1261 CCATTCATGT GCCTCCAGGT TATATCTCAC AGGTGATTGA AGATAGTACT GGAGTCCGCC 
     1321 GGGTGGTGGT CACACCCCAG TCTCCTGAGT GTTATCCCCC AAGCTACCCC TCAGCCATGT 
     1381 CTCCAACCCA TCATCTCCCT CCCTATCTGA CTCACCATCC ACATTTTATT CATAACTCAC 
     1441 ACACGGCTTA CTACCCACCT GTTACCGGAC CTGGAGATAT GCCGCCTCAG TTTTTTCCCC 
     1501 AGCATCATCT TCCCCACACA ATATATGGTG AGCAAGAAAT TATACCATTT TATGGAATGT 
     1561 CAAGCTACAT CACCCGAGAA GACCAGTACA GCAAGCCTCC GCACAAAAAA CTGAAAGACC 
     1621 GCCAGATCGA TCGCCAGAAC CGCCTCAACA GCCCTCCTTC TTCTATCTAC AAAAGCAGCT 
     1681 GCACAACAGT ATACAATGGC TATGGGAAGG GCCATAGTGG TGGAAGTGGC GGAGGCGGCA 
     1741 GCGGTAGTGG TCCCGGAATT AAGAAAACAG AGCGACGAGC AAGAAGCAGC CCAAAGTCGA 
     1801 ATGATTCAGA CTTGCAAGAA TATGAGTTGG AAGTAAAGAG GGTGCAAGAC ATTCTTTCGG 
     1861 GAATAGAGAA ACCACAGGTT TCTAATATTC AGGCAAGAGC AGTTGTGTTG TCCTGGGCTC 
     1921 CCCCTGTTGG ACTTTCCTGT GGACCCCACA GTGGTCTTTC CTTCCCCTAC AGTTACGAGG 
     1981 TGGCCTTATC AGACAAAGGA CGAGATGGAA AATACAAGAT AATTTACAGT GGAGAAGAAT 
     2041 TAGAATGTAA CCTGAAAGAT CTTAGACCAG CAACAGATTA TCATGTGAGG GTGTATGCCA 
     2101 TGTACAATTC CGTAAAGGGA TCCTGCTCCG AGCCTGTTAG CTTCACCACC CACAGCTGTG 
     2161 CACCCGAGTG TCCTTTCCCC CCTAAGCTGG CACATAGGAG CAAAAGTTCA CTAACCCTGC 
     2221 AGTGGAAGGC ACCAATTGAC AACGGTTCAA AAATCACCAA CTACCTTTTA GAGTGGGATG 
     2281 AGGGAAAAAG AAATAGTGGT TTCAGACAGT GCTTCTTCGG GAGCCAGAAG CACTGCAAGT 
     2341 TGACAAAGCT TTGTCCGGCA ATGGGGTACA CATTCAGGCT GGCCGCTCGA AACGACATTG 
     2401 GCACCAGTGG TTATAGCCAA GAGGTGGTGT GCTACACATT AGGAAATATC CCTCAGATGC 
     2461 CTTCTGCACC AAGGCTGGTT CGAGCTGGCA TCACATGGGT CACGTTGCAG TGGAGTAAGC 
     2521 CAGAAGGCTG TTCACCCGAG GAAGTGATCA CCTACACCTT GGAAATTCAG GAGGATGAAA 
     2581 ATGATAACCT TTTCCACCCA AAATACACTG GAGAGGATTT AACCTGTACT GTGAAAAATC 
     2641 TCAAAAGAAG CACACAGTAT AAATTCAGGC TGACTGCTTC TAATACGGAA GGAAAAAGCT 
     2701 GTCCAAGCGA AGTTCTTGTT TGTACGACGA GTCCTGACAG GCCTGGACCT CCTACCAGAC 
     2761 CGCTTGTCAA AGGCCCAGTT ACATCTCATG GCTTTAGTGT CAAATGGGAT CCCCCTAAGG 
     2821 ACAATGGTGG TTCAGAAATC CTCAAGTACT TGCTAGAGAT TACTGATGGA AATTCTGAAG 
     2881 CGAATCAGTG GGAAGTGGCC TACAGTGGGT CGGCTACCGA ATACACCTTC ACCCACTTGA 
     2941 AACCAGGCAC TTTGTACAAA CTCCGAGCAT GCTGCATCAG TACCGGCGGA CACAGCCAGT 
     3001 GTTCTGAAAG TCTCCCTGTT CGCACACTAA GCATTGCACC AGGTCAATGT CGACCACCGA 
     3061 GGGTTTTGGG TAGACCAAAG CACAAAGAAG TCCACTTAGA GTGGGATGTT CCTGCATCGG 
     3121 AAAGTGGCTG TGAGGTCTCA GAGTACAGCG TGGAGATGAC GGAGCCCGAA GACGTAGCCT 
     3181 CGGAAGTGTA CCATGGCCCA GAGCTGGAGT GCACCGTCGG CAACCTGCTT CCTGGAACCG 
     3241 TGTATCGCTT CCGGGTGAGG GCTCTGAATG ATGGAGGGTA TGGTCCCTAT TCTGATGTCT 
     3301 CAGAAATTAC CACTGCTGCA GGGCCTCCTG GACAATGCAA AGCACCTTGT ATTTCTTGTA 
     3361 CACCTGATGG ATGTGTCTTA GTGGGTTGGG AGAGTCCTGA TAGTTCTGGT GCTGACATCT 
     3421 CAGAGTACAG GTTGGAATGG GGAGAAGATG AAGAATCCTT AGAACTCATT TATCATGGGA 
     3481 CAGACACCCG TTTTGAAATA AGAGACCTGT TGCCTGCTGC ACAGTATTGC TGTAGACTAC 
     3541 AGGCCTTCAA TCAAGCAGGG GCAGGGCCGT ACAGTGAACT TGTCCTTTGG CAGACGCCAG 
     3601 CGTCTGCCCC TGACCCCGTC TCCACTCTCT GTGTCCTGGA GGAGGAGCCC CTTGATGCCT 
     3661 ACCCTGATTC ACCTTCTGCG TGCCTTGTAC TGAACTGGGA AGAGCCGTGC AATAACGGAT 
     3721 CTGAAATCCT TGCTTACACC ATTGATCTAG GAGACACTAG CATTACCGTG GGCAACACCA 
     3781 CCATGCATGT TATGAAAGAT CTCCTTCCAG AAACCACCTA CCGGATCAGA ATTCAGGCTA 
     3841 TAAATGAAAT TGGAGCTGGA CCATTTAGTC AGTTCATTAA AGCAAAAACT CGGCCATTAC 
     3901 CACCCTTGCC TCCTAGGCTA GAATGTGCTG CTGCTGGTCC TCAGAGCCTG AAGCTAAAAT 
     3961 GGGGAGACAG TAACTCCAAG ACACATGCGG CTGAGGACAT TGTGTACACA CTACAGCTGG 
     4021 AGGACAGAAA CAAGAGGTTT ATTTCAATCT ACAGAGGACC CAGCCACACC TACAAGGTCC 
     4081 AGAGACTGAC GGAATTCACA TGCTACTCCT TCAGGATCCA GGCAGCAAGC GAGGCTGGAG 
     4141 AAGGGCCCTT CTCAGAAACC TATACCTTCA GCACAACCAA AAGTGTCCCC CCCACCATCA 
     4201 AAGCACCTCG AGTAACACAG TTAGAAGGAA ATTCATGTGA AATTTTATGG GAGACGGTAC 
     4261 CATCAATGAA AGGTGACCCT GTTAACTACA TTCTGCAGGT ATTGGTTGGA AGAGAATCTG 
     4321 AGTACAAACA GGTGTACAAG GGAGAAGAAG CCACATTCCA AATCTCAGGC CTCCAGACCA 
     4381 ACACAGACTA CAGGTTCCGC GTATGTGCGT GTCGTCGCTG TTTAGACACC TCTCAGGAGC 
     4441 TAAGCGGAGC CTTCAGCCCC TCTGCGGCTT TTGTATTACA ACGAAGTGAG GTCATGCTTA 
     4501 CAGGGGACAT GGGGAGCTTA GATGATCCCA AAATGAAGAG CATGATGCCT ACTGATGAAC 
     4561 AGTTTGCAGC CATCATTGTG CTTGGCTTTG CAACTTTGTC CATTTTATTT GCCTTTATAT 
     4621 TACAGTACTT CTTAATGAAG ACGCGTACGC GGCCGCTCGA GCAGAAACTC ATCTCAGAAG 
     4681 AGGATCTGGC AGCAAATGAT ATCCTGGATT ACAAGGATGA CGACGATAAG GTTTAAACGG 
     4741 CCGGCCGCGG TCATAGCTGT TTCCTGAACA GATCCCGGGT GGCATCCCTG TGACCCCTCC 
     4801 CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA 
     4861 AAATTAAGTT GCATCATTTT GTCTGACTAG GTGTCCTTCT ATAATATTAT GGGGTGGAGG 
     4921 GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG AAGACAACCT GTAGGGCCTG CGGGGTCTAT 
     4981 TGGGAACCAA GCTGGAGTGC AGTGGCACAA TCTTGGCTCA CTGCAATCTC CGCCTCCTGG 
     5041 GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC GAGTTGTTGG GATTCCAGGC ATGCATGACC 
     5101 AGGCTCAGCT AATTTTTGTT TTTTTGGTAG AGACGGGGTT TCACCATATT GGCCAGGCTG 
     5161 GTCTCCAACT CCTAATCTCA GGTGATCTAC CCACCTTGGC CTCCCAAATT GCTGGGATTA 
     5221 CAGGCGTGAA CCACTGCTCC CTTCCCTGTC CTTCTGATTT TAAAATAACT ATACCAGCAG 
     5281 GAGGACGTCC AGACACAGCA TAGGCTACCT GGCCATGCCC AACCGGTGGG ACATTTGAGT 
     5341 TGCTTGCTTG GCACTGTCCT CTCATGCGTT GGGTCCACTC AGTAGATGCC TGTTGAATTG 
     5401 GGTACGCGGC CAGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC 
     5461 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA 
     5521 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 
     5581 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 
     5641 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA 
     5701 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA 
     5761 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA 
     5821 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 
     5881 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 
     5941 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG 
     6001 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 
     6061 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG 
     6121 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA 
     6181 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT 
     6241 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AACCTGAGGC 
     6301 TATGGCAGGG CCTGCCGCCC CGACGTTGGC TGCGAGCCCT GGGCCTTCAC CCGAACTTGG 
     6361 GGGGTGGGGT GGGGAAAAGG AAGAAACGCG GGCGTATTGG CCCCAATGGG GTCTCGGTGG 
     6421 GGTATCGACA GAGTGCCAGC CCTGGGACCG AACCCCGCGT TTATGAACAA ACGACCCAAC 
     6481 ACCGTGCGTT TTATTCTGTC TTTTTATTGC CGTCATAGCG CGGGTTCCTT CCGGTATTGT 
     6541 CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT AGGGTGGGCG AAGAACTCCA GCATGAGATC 
     6601 CCCGCGCTGG AGGATCATCC AGCCGGCGTC CCGGAAAACG ATTCCGAAGC CCAACCTTTC 
     6661 ATAGAAGGCG GCGGTGGAAT CGAAATCTCG TGATGGCAGG TTGGGCGTCG CTTGGTCGGT 
     6721 CATTTCGAAC CCCAGAGTCC CGCTCAGAAG AACTCGTCAA GAAGGCGATA GAAGGCGATG 
     6781 CGCTGCGAAT CGGGAGCGGC GATACCGTAA AGCACGAGGA AGCGGTCAGC CCATTCGCCG 
     6841 CCAAGCTCTT CAGCAATATC ACGGGTAGCC AACGCTATGT CCTGATAGCG ATCCGCCACA 
     6901 CCCAGCCGGC CACAGTCGAT GAATCCAGAA AAGCGGCCAT TTTCCACCAT GATATTCGGC 
     6961 AAGCAGGCAT CGCCATGGGT CACGACGAGA TCCTCGCCGT CGGGCATGCT CGCCTTGAGC 
     7021 CTGGCGAACA GTTCGGCTGG CGCGAGCCCC TGATGCTCTT CGTCCAGATC ATCCTGATCG 
     7081 ACAAGACCGG CTTCCATCCG AGTACGTGCT CGCTCGATGC GATGTTTCGC TTGGTGGTCG 
     7141 AATGGGCAGG TAGCCGGATC AAGCGTATGC AGCCGCCGCA TTGCATCAGC CATGATGGAT 
     7201 ACTTTCTCGG CAGGAGCAAG GTGAGATGAC AGGAGATCCT GCCCCGGCAC TTCGCCCAAT 
     7261 AGCAGCCAGT CCCTTCCCGC TTCAGTGACA ACGTCGAGCA CAGCTGCGCA AGGAACGCCC 
     7321 GTCGTGGCCA GCCACGATAG CCGCGCTGCC TCGTCTTGCA GTTCATTCAG GGCACCGGAC 
     7381 AGGTCGGTCT TGACAAAAAG AACCGGGCGC CCCTGCGCTG ACAGCCGGAA CACGGCGGCA 
     7441 TCAGAGCAGC CGATTGTCTG TTGTGCCCAG TCATAGCCGA ATAGCCTCTC CACCCAAGCG 
     7501 GCCGGAGAAC CTGCGTGCAA TCCATCTTGT TCAATCATGC GAAACGATCC TCATCCTGTC 
     7561 TCTTGATCGA TCTTTGCAAA AGCCTAGGCC TCCAAAAAAG CCTCCTCACT ACTTCTGGAA 
     7621 TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC TGCATAAATA AAAAAAATTA GTCAGCCATG 
     7681 GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG 
     7741 GACTATGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     7801 TGGGGACTTT CCACACCTGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     7861 TGCTGGGGAG CCTGGGGACT TTCCACACCC TAACTGACAC ACATTCCACA GCTGGTTCTT 
     7921 TCCGCCTCAG GACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC 
     7981 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA 
     8041 TTTCCCCGAA AAGTGCCACC TGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG 
     8101 GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT 
     8161 TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG 
     8221 CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG 
     8281 GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG 
     8341 GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC 
     8401 TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT 
     8461 GAGCTGATTT AACAAAAATT TAACGCGAAT TTT
//