LOCUS       RC226355                8454 bp    DNA     circular  SYN 
DEFINITION  PLEKHG4 (Myc-DDK-tagged)-Human pleckstrin homology domain containing, family G (with RhoGef domain) member 4 (PLEKHG4), transcript variant 4
ACCESSION   NM_001129729
VERSION     NM_001129729.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4611..4702 
                     /label="Myc-DDK"  
     misc_feature    4732..5317 
                     /label="PolyA signal"  
     misc_feature    4789..4808 
                     /label="Restriction Enzyme"  
     misc_feature    5593..6265 
                     /label="ColE1"  
     misc_feature    6695..7489 
                     /label="Kan/Neo"  
     misc_feature    7524..7826 
                     /label="SV40 ori"  
     misc_feature    7912..8389 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4593..4599 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4593 
                     /label="PLEKHG4"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAAAGGCCC CTGGAGAATG GGGATGAGTC CCCAGACTCT CAGGGCCATG 
     1081 CCACCGACTG GAGATTTGCT GTGTGCAGTT TCAGGGATGC CTGGGAAGAG GAGGAACCTG 
     1141 CTTCCCAGAT GCACGTTAAG GACCCAGGTC CTCCAAGACC ACCAGCCGGG GCCACCCAGG 
     1201 ATGAGGAGCT ACAGGGCAGC CCCTTGTCCA GGAAATTCCA GTTACCCCCA GCTGCAGATG 
     1261 AGTCGGGGGA TGCCCAGAGG GGCACAGTAG AAAGCTCCTC AGTCCTGTCA GAAGGGCCAG 
     1321 GCCCCTCTGG AGTGGAGAGT CTCCTATGCC CCATGTCCTC CCACCTCAGC TTGGCACAGG 
     1381 GTGAGAGTGA CACCCCAGGG GTAGGGTTGG TAGGGGACCC AGGTCCAAGC AGGGCGATGC 
     1441 CATCTGGCTT GAGCCCTGGG GCATTGGACA GCGACCCTGT GGGCCTTGGA GACCCTTTAT 
     1501 CAGAAATATC AAAGCTGCTG GAGGCAGCCC CCAGTGGATC CGGCCTCCCT AAGCCTGCTG 
     1561 ACTGCCTCCT GGCCCAAGAC CTCTGTTGGG AGCTGCTGGC CAGTGGTATG GCCACCTTGC 
     1621 CAGGGACTCG GGATGTCCAA GGCCGGGCAG TGCTGCTTCT GTGTGCCCAC AGCCCAGCCT 
     1681 GGCTTCAGTC TGAGTGCAGC AGCCAGGAAC TCATCCGCCT CCTGCTGTAC CTGCGAAGCA 
     1741 TCCCCAGGCC CGAAGTACAG GCACTGGGAC TGACAGTGCT AGTTGATGCC CGAATTTGTG 
     1801 CTCCAAGTTC TTCCCTCTTC TCTGGGCTCA GCCAACTACA AGAAGCAGCC CCAGGGGCCG 
     1861 TGTACCAGGT GCTGCTAGTG GGAAGCACGC TGCTGAAGGA AGTGCCTTCC GGGCTGCAGC 
     1921 TGGAGCAGTT GCCTTCTCAG AGCCTGCTGA CCCACATCCC AACGGCGGGG CTGCCCACTT 
     1981 CGCTAGGAGG AGGCCTGCCT TACTGCCACC AGGCCTGGCT GGATTTCCGA AGGCGGCTGG 
     2041 AAGCTCTACT ACAGAACTGC CAGGCAGCTT GTGCCCTGCT CCAGGGGGCC ATCGAAAGTG 
     2101 TGAAGGCTGT GCCCCAGCCC ATGGAGCCTG GGGAGGTCGG TCAGCTGCTA CAGCAGACAG 
     2161 AGGTCCTGAT GCAGCAGGTG CTAGACTCGC CATGGCTGGC ATGGCTACAA TGCCAGGGGG 
     2221 GCCGGGAGCT GACATGGCTG AAGCAAGAGG TCCCAGAGGT GACCCTGAGC CCAGACTACA 
     2281 GGACGGCAAT GGACAAGGCT GACGAGCTAT ATGACCGGGT GGATGGATTG CTGCACCAAC 
     2341 TGACCCTGCA GAGCAACCAG CGAATACAGG CCCTAGAGTT GGTCCAAACA CTGGAGGCCC 
     2401 GGGAAAGCGG ACTGCACCAG ATTGAAGTGT GGCTGCAGCA GGTGGGCTGG CCAGCACTGG 
     2461 AGGAGGCTGG GGAGCCCTCG CTGGACATGC TGCTCCAGGC CCAAGGCTCT TTTCAGGAGC 
     2521 TGTACCAGGT TGCCCAGGAG CAGGTCAGGC AAGGGGAGAA GTTTCTGCAG CCGCTGACTG 
     2581 GCTGGGAGGC GGCTGAACTG GACCCCCCTG GGGCACGCTT TCTGGCCCTG CGAGCCCAGC 
     2641 TGACTGAATT CTCTAGGGCT TTGGCCCAGC GGTGCCAGCG GCTGGCGGAT GCTGAGAGGC 
     2701 TGTTTCAGCT CTTCAGGGAG GCCTTGACGT GGGCTGAGGA GGGGCAGCGA GTGTTGGCAG 
     2761 AGCTGGAGCA GGAACGCCCG GGGGTTGTGT TGCAGCAGCT GCAGCTGCAC TGGACCAGGC 
     2821 ACCCTGACTT GCCTCCTGCC CACTTCCGAA AGATGTGGGC TCTGGCCACG GGGCTGGGCT 
     2881 CAGAGGCCAT CCGCCAGGAG TGCCGCTGGG CCTGGGCGCG GTGCCAGGAC ACCTGGCTGG 
     2941 CCCTGGACCA AAAGCTTGAG GCTTCACTGA AGCTACCACC GGTGGGCAGC ACAGCTAGCC 
     3001 TGTGTGTCAG CCAGGTCCCC GCTGCACCTG CCCACCCTCC CCTGAGGAAG GCCTACAGCT 
     3061 TCGATCGGAA TCTGGGGCAG AGTCTCAGTG AACCTGCCTG CCACTGCCAC CATGCGGCCA 
     3121 CTATTGCTGC CTGCCGCAGA CCAGAGGCTG GAGGAGGTGC CCTGCCCCAG GCATCCCCTA 
     3181 CTGTGCCTCC ACCAGGCAGC TCTGACCCCA GGAGCCTCAA CAGGCTACAG CTGGTGCTGG 
     3241 CAGAGATGGT GGCCACGGAG CGGGAGTATG TCCGGGCTCT AGAGTACACT ATGGAGAACT 
     3301 ATTTCCCCGA GCTGGATCGC CCCGATGTGC CCCAGGGCCT CCGCGGTCAG CGTGCCCACC 
     3361 TCTTTGGCAA CCTGGAGAAG CTGCGGGACT TCCACTGCCA CTTCTTCCTG CGTGAGCTGG 
     3421 AGGCTTGCAC CCGGCACCCA CCACGAGTGG CCTATGCCTT CCTGCGCCAT AGGGTGCAGT 
     3481 TTGGGATGTA CGCGCTCTAC AGCAAGAATA AGCCTCGCTC CGATGCCCTG ATGTCAAGCT 
     3541 ATGGGCACAC CTTCTTCAAG GACAAGCAGC AAGCACTGGG GGACCACCTG GACCTGGCCT 
     3601 CCTACCTGCT AAAGCCCATC CAGCGCATGG GCAAGTACGC ACTGCTGCTG CAGGAGCTGG 
     3661 CACGGGCCTG CGGGGGCCCC ACGCAGGAGC TCAGTGCGCT GCGGGAGGCC CAGAGCCTTG 
     3721 TGCACTTCCA GCTGCGGCAC GGAAACGACC TGCTGGCCAT GGACGCCATC CAGGGCTGTG 
     3781 ATGTTAACCT CAAGGAACAG GGGCAGCTGG TGCGACAGGA TGAGTTTGTG GTGCGCACTG 
     3841 GGCGCCACAA GTCCGTGCGC CGCATCTTCC TTTTTGAGGA GCTGCTGCTC TTCAGCAAGC 
     3901 CTCGCCATGG GCCCACAGGG GTTGACACAT TTGCCTACAA GCGCTCCTTC AAGATGGCAG 
     3961 ACCTTGGTCT CACTGAGTGC TGTGGGAACA GCAACCTGCG CTTCGAGATC TGGTTCCGCC 
     4021 GCCGCAAGGC CAGGGACACC TTTGTGCTGC AGGCCTCCAG CCTGGCTATC AAGCAGGCCT 
     4081 GGACAGCTGA CATCTCCCAC CTGCTTTGGA GGCAGGCCGT CCACAACAAG GAGGTGCGCA 
     4141 TGGCTGAGAT GGTGTCCATG GGTGTGGGGA ACAAGGCCTT CCGAGACATT GCTCCCAGCG 
     4201 AGGAAGCCAT CAACGACCGC ACCGTCAACT ATGTCCTGAA GTGCCGAGAA GTTCGCTCTC 
     4261 GGGCGTCCAT TGCCGTAGCC CCGTTTGACC ATGACAGCCT CTACCTGGGG GCCTCGAACT 
     4321 CCCTTCCTGG AGACCCTGCC TCTTGCTCTG TTCTGGGGTC CCTCAACCTG CACCTGTACA 
     4381 GAGACCCAGC TCTTCTGGGT CTCCGCTGTC CCCTGTATCC CAGCTTCCCA GAGGAAGCAG 
     4441 CACTGGAGGC TGAGGCAGAG CTGGGCGGCC AGCCCTCTTT GACTGCTGAG GACTCAGAGA 
     4501 TCTCGTCCCA ATGCCCATCA GCCAGTGGCT CCAGTGGCTC TGACAGCAGC TGTGTGTCAG 
     4561 GGCAGGCCCT GGGTAGGGGC CTGGAGGACT TACCCTGTGT CACGCGTACG CGGCCGCTCG 
     4621 AGCAGAAACT CATCTCAGAA GAGGATCTGG CAGCAAATGA TATCCTGGAT TACAAGGATG 
     4681 ACGACGATAA GGTTTAAACG GCCGGCCGCG GTCATAGCTG TTTCCTGAAC AGATCCCGGG 
     4741 TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG 
     4801 CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC 
     4861 TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC 
     4921 TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC 
     4981 ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG 
     5041 GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT 
     5101 TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG 
     5161 CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT 
     5221 TTAAAATAAC TATACCAGCA GGAGGACGTC CAGACACAGC ATAGGCTACC TGGCCATGCC 
     5281 CAACCGGTGG GACATTTGAG TTGCTTGCTT GGCACTGTCC TCTCATGCGT TGGGTCCACT 
     5341 CAGTAGATGC CTGTTGAATT GGGTACGCGG CCAGCGGCGA GCGGTATCAG CTCACTCAAA 
     5401 GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA 
     5461 AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT 
     5521 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 
     5581 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 
     5641 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 
     5701 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 
     5761 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 
     5821 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 
     5881 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 
     5941 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 
     6001 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 
     6061 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC 
     6121 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC 
     6181 AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG 
     6241 TATATATGAG TAACCTGAGG CTATGGCAGG GCCTGCCGCC CCGACGTTGG CTGCGAGCCC 
     6301 TGGGCCTTCA CCCGAACTTG GGGGGTGGGG TGGGGAAAAG GAAGAAACGC GGGCGTATTG 
     6361 GCCCCAATGG GGTCTCGGTG GGGTATCGAC AGAGTGCCAG CCCTGGGACC GAACCCCGCG 
     6421 TTTATGAACA AACGACCCAA CACCGTGCGT TTTATTCTGT CTTTTTATTG CCGTCATAGC 
     6481 GCGGGTTCCT TCCGGTATTG TCTCCTTCCG TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC 
     6541 GAAGAACTCC AGCATGAGAT CCCCGCGCTG GAGGATCATC CAGCCGGCGT CCCGGAAAAC 
     6601 GATTCCGAAG CCCAACCTTT CATAGAAGGC GGCGGTGGAA TCGAAATCTC GTGATGGCAG 
     6661 GTTGGGCGTC GCTTGGTCGG TCATTTCGAA CCCCAGAGTC CCGCTCAGAA GAACTCGTCA 
     6721 AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA TCGGGAGCGG CGATACCGTA AAGCACGAGG 
     6781 AAGCGGTCAG CCCATTCGCC GCCAAGCTCT TCAGCAATAT CACGGGTAGC CAACGCTATG 
     6841 TCCTGATAGC GATCCGCCAC ACCCAGCCGG CCACAGTCGA TGAATCCAGA AAAGCGGCCA 
     6901 TTTTCCACCA TGATATTCGG CAAGCAGGCA TCGCCATGGG TCACGACGAG ATCCTCGCCG 
     6961 TCGGGCATGC TCGCCTTGAG CCTGGCGAAC AGTTCGGCTG GCGCGAGCCC CTGATGCTCT 
     7021 TCGTCCAGAT CATCCTGATC GACAAGACCG GCTTCCATCC GAGTACGTGC TCGCTCGATG 
     7081 CGATGTTTCG CTTGGTGGTC GAATGGGCAG GTAGCCGGAT CAAGCGTATG CAGCCGCCGC 
     7141 ATTGCATCAG CCATGATGGA TACTTTCTCG GCAGGAGCAA GGTGAGATGA CAGGAGATCC 
     7201 TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG TCCCTTCCCG CTTCAGTGAC AACGTCGAGC 
     7261 ACAGCTGCGC AAGGAACGCC CGTCGTGGCC AGCCACGATA GCCGCGCTGC CTCGTCTTGC 
     7321 AGTTCATTCA GGGCACCGGA CAGGTCGGTC TTGACAAAAA GAACCGGGCG CCCCTGCGCT 
     7381 GACAGCCGGA ACACGGCGGC ATCAGAGCAG CCGATTGTCT GTTGTGCCCA GTCATAGCCG 
     7441 AATAGCCTCT CCACCCAAGC GGCCGGAGAA CCTGCGTGCA ATCCATCTTG TTCAATCATG 
     7501 CGAAACGATC CTCATCCTGT CTCTTGATCG ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA 
     7561 GCCTCCTCAC TACTTCTGGA ATAGCTCAGA GGCCGAGGCG GCCTCGGCCT CTGCATAAAT 
     7621 AAAAAAAATT AGTCAGCCAT GGGGCGGAGA ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG 
     7681 GATGGGCGGA GTTAGGGGCG GGACTATGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     7741 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCTG GTTGCTGACT AATTGAGATG 
     7801 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC CTAACTGACA 
     7861 CACATTCCAC AGCTGGTTCT TTCCGCCTCA GGACTCTTCC TTTTTCAATA TTATTGAAGC 
     7921 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA 
     7981 CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC 
     8041 GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC 
     8101 CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC 
     8161 CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC 
     8221 GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG 
     8281 GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT 
     8341 GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT 
     8401 TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
//